scholarly journals Isolation of Ethyl p-Methoxycinnamate from Azadirachta indica Root Bark as Hong Kong Caterpillar (Tenebrio molitor) Antifeedant

2021 ◽  
Vol 21 (6) ◽  
pp. 1348
Author(s):  
Rosnani Nasution ◽  
Cut Nyak Candein ◽  
Nurdin Saidi ◽  
Muhammad Bahi ◽  
Marianne Marianne
Keyword(s):  
Hong Kong ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Azadirachta Indica ◽  
Ethyl Acetate Extract ◽  
Tenebrio Molitor ◽  
Root Bark ◽  
Antifeedant Activity ◽  
Synthetic Pesticides ◽  
Disk Method

This study aims to identify the antifeedant activity of Azadirachta indica A. Juss root bark against Hong Kong caterpillar (Tenebrio molitor). A. indica A. root was macerated in n-hexane, ethyl acetate, and methanol. The investigation of antifeedant activity was conducted by using the no choice leaf disk method. The antifeedant activity (%AI) tests showed that the extracts of n-hexane, ethyl acetate, and methanol with each concentration of 0.5% were 6.71, 71.78, and 40.14%, respectively. The ethyl acetate extract demonstrated the most active antifeedant activity. Ethyl acetate extract was fractionated to obtain five subfractions (A-E). Each subfraction with each concentration of 0.5% showed the %AI of 70.55, 85.29; 67.40, 82.70 and 82.22%, respectively. Furthermore, GC-MS results demonstrated that ethyl acetate extract contained 19 compounds. The main compounds are methyl hexadecanoate and methyl 8-octadecenoate. Further purification of fraction A, which has the highest amount, was then carried out. The obtained isolate, i.e., A32, was determined as ethyl p-methoxycinnamate confirmed by 13C- and 1H-NMR, FTIR, and MS spectra. The presence of ethyl p-methoxycinnamate makes A. indica A. root is potential as T. molitor antifeedant. The implication of these findings is to reference herbal antifeedants and reduce the use of synthetic pesticides.

scholarly journals ISOLATION AND IDENTIFICATION OF ANANIXANTHONE FROM ETHYL ACETATE EXTRACT OF ROOT BARK OF SLATRI (Calophyllum soulattri) (CLUSIACEAE))

2016 ◽  
Vol 10 (2) ◽  
pp. 130
Author(s):  
Dindha Ramah Mulia ◽  
Nestri Wulandari ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Ethyl Acetate Extract ◽  
Root Bark ◽  
Isolation And Identification

<p><em></em>A  xanthone,  named  ananixanthone  (1)  has  been  isolated  and  identified  from  the  ethyl acetate  extract of the root barks of  Calophyllum soulattri. Structure of the compound was determined based on spectroscopic data, including UV, IR, NMR 1D, NMR 2D and by comparison with references.</p>

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES

2019 ◽  
pp. 151-154
Author(s):  
GAURAV SHARMA ◽  
ANKITA THAKUR ◽  
SOHAN LAL ◽  
ROHIT KUMAR NADDA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Comparative Evaluation ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed. Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous. Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.

scholarly journals Triterpenoids from the Stem Bark of Crataeva nurvala

1970 ◽  
Vol 7 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Md Enamul Haque ◽  
Md Nahidul Islam ◽  
Dipankar Das Gupta ◽  
Mahbub Hossain ◽  
Hossain Uddin Shekhar ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Silica Gel ◽  
1H Nmr ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
First Report ◽  
Spectroscopic Analyses ◽  
Phytochemical Investigation

Two triterpenoids, phragmalin triacetate (1) and lupeol (2) were isolated from an ethyl acetate extract of the stem bark of Crataeva nurvala (Capparidaceae) by repeated chromatography over silica gel. The structures of these compounds were determined by spectroscopic analyses (UV, IR, 1H NMR, 13C NMR and EIMS). This is the first report of the systematic phytochemical investigation and the presence of these compounds 1 and 2 from this plant. Key words: Crataeva, Capparidaceae, Phragmalin triacetate and Lupeol.  DOI = 10.3329/dujps.v7i1.1221 Dhaka Univ. J. Pharm. Sci. 7(1): 71-74, 2008 (June)

scholarly journals Antiproliferative Activity of Triterpenoid and Steroid Compounds from Ethyl Acetate Extract of Calotropis gigantea Root Bark against P388 Murine Leukemia Cell Lines

2021 ◽  
Vol 89 (2) ◽  
pp. 21
Author(s):  
Kartini Hasballah ◽  
Murniana Sarong ◽  
Renzavaldy Rusly ◽  
Herdina Fitria ◽  
Dewi Rara Maida ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Ethyl Acetate Extract ◽  
Leukemia Cell ◽  
Root Bark ◽  
Leukemia Cell Lines ◽  
Murine Leukemia Cell ◽  
Murine Leukemia ◽  
Antiproliferative Activities

Calotropis gigantea has been known to produce bioactive secondary metabolites with antiproliferative activities against cancer cells. Herein, we extracted the secondary metabolites using ethyl acetate from its root bark and further tested its antiproliferative activities against P388 murine leukemia cell lines. The subfractions from the ethyl acetate extract was obtained from Vacuum Liquid Column Chromatography (VLCC), and followed by Gravity Column Chromatography (GCC). The subfraction C2 and D1 were identified to contain triterpenoids and steroids with the most potent cytotoxicity against Brine Shrimp Lethality Test (BSLT). A 3-(4,5-dimethylthiazol-2-yl) -2-5 diphenyl tetrazolium bromide (MTT) assay suggested that ethyl acetate extract has the highest antiproliferative activities against P388 murine leukemia cell lines (IC50 = 21.79 μg/mL), as opposed to subfraction C2 (IC50 = 50.64 µg/mL) and subfraction D1 (IC50 = 49.33 µg/mL). The compound identified in subfraction C2 and D1 are taraxerol acetate and calotropone, respectively. Though taraxerol acetate and calotropone were active in inhibiting the leukemic cell lines, their IC50s were lower than the ethyl acetate extract, which is probably due to the synergism of the secondary metabolites.

scholarly journals ISOLASI KUMARIN DARI KULIT BUAH LIMAU SUNDAI (Citrus nobilis Lour)

2017 ◽  
Vol 18 (02) ◽  
pp. 137-145
Author(s):  
Melindra Mulia
Keyword(s):  
Melting Point ◽  
Ir Spectra ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
White Needle ◽  
Pure White

Coumarin has been isolated and characteritated from rind of  Citrus nobilis Lour by maseration methode with methanol. After fractionation by n-hexane and ethyl acetate, collected the phase ethyl acetate which positive of coumarin. From ethyl acetate extract coumarin have been isolated by column chromatography. The isolation results was obtain 2,159 g of pure white needle-shape crystalline with the melting point of 126,2-127,60C. Structure of the isolated coumarine was elucidated by spectroscopic methodes, UV-Vis,13C-NMR, 1H-NMR, 2D-NMR (DEPT/HSQC, COSY, NOESY, HMBC) and IR spectra. Based on the spectra data the isolated coumarine is marmin.

scholarly journals ISOLATION AND IDENTIFICATION OF β-SITOSTEROL AND STIGMASTEROL MIXTURE FROM ROOT BARK OF SLATRI (Calophyllum soulattri Burm. f)

2018 ◽  
Vol 10 (1) ◽  
pp. 87
Author(s):  
Doni Eko Saputra ◽  
Nestri Handayani ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flash Chromatography ◽  
Root Bark ◽  
Isolation And Identification ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Yellowish White ◽  
C Nmr

<p>Chemical isolation and identificationof root bark of <em>Calophyllumsoulattri</em>Burm. f. have been conducted. Isolation was conducted by maceration with ethyl acetate as solvent. Ethyl acetate extract was separated and purified by vacuum liquid chromatography and flash chromatography which was guided by thin layer chromatography to obtain yellowish white solid. Isolated compounds were determined by spectroscopy methods such as UV, FTIR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, DEPT, HSQC, HMBC and were compared to the references. Based on the analysis result it’s known that the compounds are the mixture of?-sitosterol and stigmasterol.</p>

scholarly journals ISOLASI SIKLOKOMUNOL DARI DAUN SUKUN Artocarpus altilis (PARKINSON) FOSBERG SERTA AKTIVITASNYA SEBAGAI ANTIKANKER

2014 ◽  
Vol 16 (2) ◽  
pp. 82-86 ◽  
Author(s):  
Chandra Risdian ◽  
Tjandrawati Mozef ◽  
Puspa D.N Lotulung
Keyword(s):  
Liver Cirrhosis ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
1H Nmr ◽  
13C Nmr ◽  
Anticancer Agent ◽  
Ethyl Acetate Extract ◽  
Gradient Elution ◽  
Artocarpus Altilis ◽  
Mcf 7

Daun sukun (Artocarpus altilis) sudah lama dijadikan sebagai obat tradisional untuk mengatasi berbagai penyakit seperti liver cirrhosis, hipertensi, dan diabetes. Ekstrak etil asetat dari daun sukun juga berpotensi menghambat pertumbuhan sel kanker. Beberapa senyawa yang telah diketahui sebagai agen antikanker di dalam ekstrak etil asetat daun sukun adalah golongan geranil flavonoid. Akan tetapi, senyawa pyranoflavoid belum dilaporkan dapat ditemukan pada daun sukun. Untuk itu, penelitian ini bertujuan untuk mengisolasi senyawa pyranoflavonoid dari ekstrak etil asetat daun sukun dan menguji aktivitasnya sebagai antikanker. Daun sukun diekstraksi dengan etanol 70% kemudian dilakukan partisi dengan petroleum eter dan etil asetat. Ekstrak etil asetat yang diperoleh difraksinasi dengan kromatografi kolom silica gel dengan gradient elusi heksan-etil asetat sehingga diperoleh senyawa kristal berwarna kuning, AA3. Senyawa AA3 kemudian diidentifikasi dengan spektrofotometer UV-Vis, LC-MS, 1H-NMR dan 13C-NMR dan diperoleh hasil bahwa senyawa AA3 tersebut adalah siklokomunol yang merupakan golongan pyranoflavonoid. Uji antikanker siklokomunol dengan MCF-7 dan T47D menunjukkan adanya aktivitas antikanker dengan nilai IC50 masing-masing adalah 75.46 µM dan 36.20 µM selama 48 jam.Kata kunci:Artocarpus altilis, siklokomunol, antikanker, MCF-7, T47D. Leaves of breadfruit Artocarpus altilis (Parkinson) Fosberg has long been used as traditional medicine to overcome a variety of diseases such as liver cirrhosis, hypertension, and diabetes. Ethyl acetate extract of the leaves of breadfruit also potentially inhibit the growth of cancer cells. Several compounds have been known as an anticancer agent in the ethyl acetate extract of leaves of breadfruit is belong to geranyl flavonoid group. However, the compound belong to pyranoflavoid group has not been reported can be found on the leaves of breadfruit. Therefore, this study aims to isolate the pyranoflavonoid compound from ethyl acetate extract of leaves of breadfruit and tested as an anticancer agent. Breadfruit leaves were extracted with 70% ethanol and then made a partition with petroleum ether and ethyl acetate. Ethyl acetate extract obtained was fractionated by silica gel column chromatography with gradient elution of hexane-ethyl acetate to obtain a yellow crystalline compound, AA3. AA3 compound was identified by UV-Vis spectrophotometer, LC-MS, 1H-NMR and 13C-NMR and obtained results that AA3 compound is siklokomunol which is belong to pyranoflavonoid group. Anticancer test of siklokomunol with MCF-7 and T47D showed anticancer activity with IC50 values of each are 75.46 µM and 36.20 µM, respectively, for 48 hours.Keywords: Artocarpus altilis, siklokomunol, anticancer, MCF-7, T47D.

Isolation and Structure Determination of Antioxidants Active Compounds from Ethyl Acetate Extract of Heartwood Namnam (Cynometra cauliflora L.)

2017 ◽  
Vol 19 (1) ◽  
pp. 11-17
Author(s):  
Dede Sukandar ◽  
Siti Nurbayti ◽  
Tarso Rudiana ◽  
Tsalats Wahyul Husna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aromatic Ring ◽  
1H Nmr ◽  
Ethyl Acetate Extract ◽  
Active Compounds ◽  
Chromophore Group ◽  
Ic50 Value ◽  
High Antioxidant Activity

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring at band I (λmax 330.22 nm), chromophore group of alkene (C=C) at band II (λmax 268.67 nm) and functional groups such as O−H (3343.91 cm-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1), C−O (1269.89) and C−H ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at δH 6.86 ppm (1H, s), C-6 at δH 5.95 ppm (1H, d, J=1.95 Hz), C-8 at δH 6.25 ppm (1H, d, J= 1.95 Hz), C-2’ and C-6’ at δH 7.03 ppm (2H, d, J=7.87 Hz), C-3’ and C-5’ at δH 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC50 value of 5.18 ± 0.014 ppm.

scholarly journals Stigmasterol, rengyolone, 2-phenylethyl β-D-glucopyranoside and n-tetradecyl-β-D-glucopyranoside from the flowers of Nyctanthes arbor-tristis Linn

2019 ◽  
Vol 54 (3) ◽  
pp. 275-282 ◽  
Author(s):  
MM Haque ◽  
N Sultana ◽  
SMT Abedin ◽  
SE Kabir
Keyword(s):  
Ethyl Acetate ◽  
1H Nmr ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Natural Sources ◽  
Chromatographic Techniques ◽  
Phytochemical Investigation ◽  
First Time

A phytochemical investigation was conducted on the flowers of Nyctanthesarbor-tristis Linn. For isolation of compounds, the dried flower’s powder was successively extracted with n-hexane, dichloromethane, ethyl acetate and methanol. The extracts were fractionated using different chromatographic techniques and four compounds were isolated. Stigmasterol (1) from n-hexane, rengyolone (2) from dichloromethane and two other compounds namely, 2-phenylethyl β-D-glucopyranoside (3) and n-tetradecyl-β-D-glucopyranoside (4) from ethyl acetate extract, were isolated. These compounds (1-4) were characterized on the basis of IR, 1H NMR, 13C NMR, DEPT-135 NMR. Compounds 1 and 3 were isolated for the first time from this plant while compound 4 has been isolated and completely characterized from this plant as well as from the natural sources. Bangladesh J. Sci. Ind. Res.54(3), 275-282, 2019

Sign in / Sign up

Export Citation Format

Share Document