scholarly journals Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant)

2020 ◽  
Vol 25 (2) ◽  
pp. 94
Author(s):  
Sesilia Rante Pakadang ◽  
Santi Sinala ◽  
Alfrida Monica Salasa ◽  
St Ratnah ◽  
Sisilia Teresia Rosmala Dewi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Effective Dose ◽  
Leaf Extract ◽  
Sputum Sample ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Enterobacter Agglomerans ◽  
Dpph Method

Research has been conducted on the treatment of phlegm cough with miana leaf extract in vitro (effective dose of miana leaf extract as an expectorant and antibacterial agent causing cough with phlegm). The study aims to compare the antioxidant activity of miana leaves from 3 locations where it grows, determine the types of contaminant bacteria in the sputum of cough sufferers, determine the minimum value of inhibitor concentration (MIC) and MKC of miana leaves against the test bacteria causing cough with phlegm, determine the effective dose of miana leaves that can used as a reference for coughing up phlegm and proving the potential of miana leaves as a sputum thinner. Miana leaf extraction is done by the juicer method. Antioxidant activity testing uses the DPPH method. Determination of test bacteria is done by isolating and identifying contaminant bacteria in the sputum sample of cough with phlegm. Testing the effectiveness of miana leaves against test bacteria is determined by the liquid dilution method. Expectorant activity testing was determined by measuring the viscosity of mucus viscosity of cow intestine treated with miana leaf extract. The results found that antioxidant activity was influenced by the location where miana leaves grew with an antioxidant potential of IC72 0.072 mg/ml - 0.76 mg/ml. Contaminant bacteria from sputum samples of cough patients are Streptococcus pneumonia, Klebsiella pneumonia, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter agglomerans. MIC values for contaminant bacteria are 0.1% - 0.75% and MKC values are found between 0.25% - 1.75%. miana leaf extract has the potential as a sputum thinner at a concentration of 0.01% - 0.1%. The recommended dose of miana leaf extract as a cough with phlegm is 1.75% w/v.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

scholarly journals IN VITRO ANTIBACTERIAL EFFICACY OF PLECTRANTHUS AMBOINICUS MEDIATED SILVER NANOPARTICLES AGAINST URINARY TRACT PATHOGENS

2019 ◽  
pp. 153-159
Author(s):  
SREELAKSHMY S ◽  
THANGAPANDIYAN S
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Plate Count ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Plectranthus Amboinicus

Objective: The objective of the present study is to synthesize green mediated silver nanoparticles (AgNPs) using Plectranthus amboinicus leaf extract and to evaluate its antibacterial activity against selected urinary tract infection (UTI) causing bacteria. Methods: Phytoconstituents present in leaf extract of P. amboinicus (Lour.) Spreng were analyzed by standard qualitative tests. The synthesized AgNPs were characterized by visual inspection, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy. The antibacterial activity of synthesized AgNPs was examined against the UTI pathogens such as Pseudomonas aeruginosa, Enterobacter sp., Klebsiella pneumonia, and Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were carried out by broth dilution method and standard plate count method, respectively. Results: Synthesized AgNPs exhibited a spherical shape with an average size of 17.3 nm. The antibacterial activity of AgNPs against tested bacterial pathogens showed a maximum inhibition zone of 22.00±1.00 mm against P. aeruginosa. The bacterial growth inhibition was confirmed by MIC and MBC. Conclusion: P. amboinicus leaf extract mediated AgNPs could be used as an effective antibacterial agent for the management of UTIs. Surface modifications of urinary catheters with AgNPs may prevent the risk of contamination and the associated infections.

scholarly journals Evaluation of the Antioxidant Activity of Stalk and Fruit of Solanum aethiopicum L. (Solanaceae)

2020 ◽  
pp. 6-12
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Ethanol Extract ◽  
New Drugs ◽  
Solanum Aethiopicum ◽  
Dpph Method ◽  
The Many

Background: The use of plants for healing dates back to very remote times. Nowadays with the accession of new diseases plants are increasingly used for the formulation of new drugs able to overcome the many diseases (cancer, atherosclerosis) often caused by the disorder of the system prooxidant/antioxidant. Aim/Objective: On the strength of this observation, the research of an antioxidant plant is essential, hence the aim of this study, which is to determine the antioxidant activity of the stalk and the fruit of Solanum aethiopicum L. Methods: The fruits and stalk were washed, cut into fine slats, then dried in the incubator for three days and finally crushed into powder. An extraction by decoction with ethanol (stalks and fruits) and water (fruit) was subsequently carried out to obtain three extracts (ethanol and water). Antioxidant activity was evaluated through the FRAP method, and the trapping of radical DPPH. Results: For the FRAP method, at the highest concentration (1 mg/ml) the aqueous extract of the fruit (74.84±2.97%) has a higher reducing power compared to those of the ethanolic extracts of the fruit (70.15 ± 5.72%) and the stalk (49.85 ± 2.11%). These reducing powers, although significant, remain lower than those of tannic acid (89.95±0.007%). And finally, for the DPPH method, the aqueous extract of the fruit is more effective in reducing free radical DPPH with a IC50= 162±33 µg/ml, follow up by ethanol extract from the stalk (IC50= 360± 90 µg/ml) and finally ethanol extract from the fruit (IC50= 362.5± 23.5 µg/ml). These results confirm the in vitro antioxidant activity of the studied parts of Solanum aethiopicum. Conclusion: Prospective studies could focus on acute and subacute toxicities and the determination of the molecules responsible for the activity.

scholarly journals Antioxidant activity, xanthine oxidase inhibitory activity, and compounds determination of Cipadessa baccifera leaf extract

2021 ◽  
Author(s):  
Syarifah Ramadhani Lubis ◽  
Subandi Subandi ◽  
Muntholib Muntholib ◽  
Jamilah Abbas ◽  
Tjandrawati Mozef
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Leaf Extract

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2739
Author(s):  
Liza Devita ◽  
Hanifah Nuryani Lioe ◽  
Mala Nurilmala ◽  
Maggy T. Suhartono
Keyword(s):  
Antioxidant Activity ◽  
In Silico ◽  
Antioxidant Activities ◽  
In Silico Analysis ◽  
Weight Fraction ◽  
Amino Acid Sequences ◽  
Skin Collagen ◽  
Dpph Method ◽  
Peptide Fractions

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

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