scholarly journals ANTIBACTERIAL ACTIVITY OF FRUIT BANANA STONE AND MECHANISM

2018 ◽  
Vol 11 (13) ◽  
pp. 167
Author(s):  
Panal Sitorus ◽  
Dwi Suryanto, Hepni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Clear Zone ◽  
Activity Test ◽  
Cellular Metabolite ◽  
Cell Leakage

Objective: The purpose of this study was to determine the strength of antibacterial activity of the fraction of banana stone and its mechanism.Methods: The antibacterial activity test was performed using the diffusion method by measuring the diameter of the clear zone around the disc paper.Results: The results of antibacterial activity test showed that ethyl acetate fraction was more effective against bacterium Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes compared to n-hexane fraction.Conclusion: The resulting cellular metabolite leak showed that cell leakage in the three bacteria due to ethyl acetate fraction had leaked more protein than nucleic acid, while the leakage of more dissolved K+ ion than Ca2 + ions.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antibakteri Fraksi Etil Asetat dan n-Heksana dari Daun Mangga Kasturi (Mangifera casturi Kosterm.)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 227-233
Author(s):  
Dwi Lestari ◽  
Desy Fitriani ◽  
Serli Anngraeni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Study Results ◽  
Mango Leaves ◽  
Weak Antibacterial Activity

Kasturi mango (Mangifera casturi Kosterm.) is a typical mango of South Kalimantan. Kasturi mango leaves are reported to have antioxidant activity and are potential for treating various diseases, including diseases related to antibacterial. This study examines the antibacterial activity of ethyl acetate fraction and the n-hexane fraction of mango musk leaves on bacteria that cause acne. Experimental research started with plant determination, making extracts and fractions, phytochemical screening, and antibacterial testing against Staphylococcus aureus and Propionibacterium acnes using the disk diffusion method. The study results found that the ethyl acetate and n-hexane fractions had weak antibacterial activity against S. aureus and P. acnes bacteria, which cause acne

scholarly journals Antibacterial Activity of Extract and Fraction From Shitake Mushroom (Lentinula edodes) Against Acne Bacteria

2018 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Ika Kurnia Sukmawati ◽  
Ari Yuniarto ◽  
Widhya Alighita ◽  
Ade Zam-zam J
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Lentinus Edodes ◽  
Shiitake Mushrooms ◽  
And Staphylococcus Aureus

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM

scholarly journals ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 5 (1) ◽  
pp. 31
Author(s):  
Sekar Wulandari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Diffusion Method ◽  
Resistance Response ◽  
Activity Test ◽  
Significant Difference

Background: Staphylococcus aureus is a gram-positive bacteria that can cause infection. One of the plants that has antibacterial activity is jatropha leaves which contain flavonoids, saponins, tannins, alkaloids, steroids and polyphenols. Purpose: To determine the antibacterial activity of ethanol extract and ethyl acetate fraction with concentrations of 30%, 60% and 100% against Staphylococcus aureus bacteria. Method: The method for extracting jatropha leaves is maceration with 96% ethanol solvent and the fractionation method, namely liquid-liquid fractionation with ethyl acetate solvent. Antibacterial activity test was carried out in vitro with the disc diffusion method and compared the mean zone of inhibition of each treatment with a positive control (gentamicin 10 μg). Result: The results showed that the ethanol extract and ethyl acetate fraction of jatropha leaves had a strong resistance response, while the positive control gave a very strong inhibitory response to the growth of S. aureus bacteria. Based on the one way ANOVA test, ethanol extract and ethyl acetate fraction showed a significant difference from each treatment with a significant value (P=<0.05). Conclusion: The ethanol extract of Jatropha leaves can inhibit the growth of S. aureus bacteria at a concentration of 100% (18.28 ± 0.50 mm), 100% concentration of ethyl acetate fraction (15.10 ± 0.12 mm). The ethanol extract provided the best inhibition power, namely 18.28 ± 0.50 mm and a positive control 21.82 ± 0.092 mm.

scholarly journals Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes

2021 ◽  
Vol 6 (1) ◽  
pp. 58528
Author(s):  
Vilya Syafriana ◽  
Renita Noviani Purba ◽  
Yayah Siti Djuhariah
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Etlingera Elatior

This study aimed to determine the antibacterial activity from the ethanol extract of Kecombrang flower (Etlingera elatior (Jack) R.M. Smith) against Staphylococcus epidermidis and Propionibacterium acnes. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the disk diffusion method with a concentration of 10%, 20%, 40%, and80%. Meanwhile, theMinimum InhibitoryConcentration(MIC)was done atconcentrations of 10%, 8%, 6%, 4%, and 2%. The results showed that the Kecombrang flower (Etlingera elatior (Jack) R.M.Smith) extract hadantibacterialactivityagainstS.epidermidisand P. acnes. The MIC for S. epidermidis is at a concentration of 4%, while in P.acnes cannot determine yet.

scholarly journals STRUCTURE CHARACTERIZATION AND EVALUATION POTENTIAL OF ANTIMICROBIAL EXTRACTS FROM PHELLINUS LINTEUS AGAINST SKIN INFECTIOUS PATHOGENS, STAPHYLOCOCCUS EPIDERMIDIS ATCC12228 AND PROPIONIBACTERIUM ACNES DMST14916

2017 ◽  
Vol 9 (12) ◽  
pp. 78
Author(s):  
Surachai Techaoei ◽  
Pattaranut Eakwaropas ◽  
Khemjira Jarmkom ◽  
Warachate Khobjai
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Structure Characterization ◽  
Diffusion Method ◽  
Phellinus Linteus ◽  
Chemical Profile

Objective: The objective of this study was to investigate the antimicrobial activity of Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis ATCC12228 and Propionibacterium acnes DMST 14916.Methods: Fungal fruiting bodies were extracted with 95% ethanol and ethyl acetate, and then, vaporized. The antimicrobial activities were determined by the disc diffusion method against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 skin infectious pathogens. A minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) for those crude extracts were determined. Finally, the chemical profile of crude extract was determined by using thin layer chromatography and GC-MS.Results: The result demonstrated that the ethanolic extraction had more active fractions with an MIC of 0.5 mg/ml against the growth of Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 and also showed a minimum inhibitory concentration (MBC) at a concentration of 1.0 mg/ml, while ethyl acetate-based solvents failed to develop on TLC according to Retention factor (Rf) values of 0.71-0.76. The GC-MS was applied to investigate the chemical profile of crude extract of Phellinus linteus, revealing a component of hexadecanoic acid and 9, 12-octadecadienoic acid.Conclusion: Phellinus linteus fruiting body extracts have great potential as antimicrobial compounds against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228. Thus, they can be used in the treatment of infectious diseases caused by bacterial pathogens. 

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

scholarly journals Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

2017 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Naniek Widyaningrum ◽  
Badie'ah Badie'ah ◽  
Sri Lestari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Staphylococcus Epidermidis ◽  
Economic Value ◽  
Diffusion Method ◽  
Tea Leaves ◽  
Green Tea Leaves

The dregs of green tea leaves have not been used until today, it is still considered as a waste that is not useful. It is known that the dregs of the green tea leaves contain Epigallocatechin-3-gallate (EGCG), which is quite high. EGCG has benefits as an antibacterial. The long-term goal of this research is the application of cosmetic preparation products from the dregs of green tea leaves that have effectiveness, stability and high economic value. The specific target of this research is to get the dose and concentration of the dregs of green tea leaves in a stable and effective way as anti-acne against Staphylococcus epidermidis (S. epidermidis). The search of the dregs of green tea leaves is by techniques of infundation of liquid fractions with ethyl acetate, identification of EGCG compound by using HPLC, antibiotics test against S. epidermidis was conducted by diffusion method. The results showed that the content of EGCG of the dregs of green tea leaves was 21.325% w/w, and the concentration of 1% of the dregs of green tea leaves can inhibit the growth of S. epidermidis well.

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