scholarly journals Skrining Fitokimia dan Uji Aktivitas Antibakteri Fraksi Etil Asetat dan n-Heksana dari Daun Mangga Kasturi (Mangifera casturi Kosterm.)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 227-233
Author(s):  
Dwi Lestari ◽  
Desy Fitriani ◽  
Serli Anngraeni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Study Results ◽  
Mango Leaves ◽  
Weak Antibacterial Activity

Kasturi mango (Mangifera casturi Kosterm.) is a typical mango of South Kalimantan. Kasturi mango leaves are reported to have antioxidant activity and are potential for treating various diseases, including diseases related to antibacterial. This study examines the antibacterial activity of ethyl acetate fraction and the n-hexane fraction of mango musk leaves on bacteria that cause acne. Experimental research started with plant determination, making extracts and fractions, phytochemical screening, and antibacterial testing against Staphylococcus aureus and Propionibacterium acnes using the disk diffusion method. The study results found that the ethyl acetate and n-hexane fractions had weak antibacterial activity against S. aureus and P. acnes bacteria, which cause acne

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

scholarly journals ANTIBACTERIAL ACTIVITY OF FRUIT BANANA STONE AND MECHANISM

2018 ◽  
Vol 11 (13) ◽  
pp. 167
Author(s):  
Panal Sitorus ◽  
Dwi Suryanto, Hepni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Clear Zone ◽  
Activity Test ◽  
Cellular Metabolite ◽  
Cell Leakage

Objective: The purpose of this study was to determine the strength of antibacterial activity of the fraction of banana stone and its mechanism.Methods: The antibacterial activity test was performed using the diffusion method by measuring the diameter of the clear zone around the disc paper.Results: The results of antibacterial activity test showed that ethyl acetate fraction was more effective against bacterium Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes compared to n-hexane fraction.Conclusion: The resulting cellular metabolite leak showed that cell leakage in the three bacteria due to ethyl acetate fraction had leaked more protein than nucleic acid, while the leakage of more dissolved K+ ion than Ca2 + ions.

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

scholarly journals PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF HEXANE AND ETHYL ACETATE EXTRACT OF KNOXIA SUMATRENSIS (RETZ.) DC.

2021 ◽  
pp. 35-39
Author(s):  
LOGANATHAN S ◽  
SELVAM K
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Standard Procedure ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Near Future ◽  
Ethyl Acetate Extracts

Objective: The study was carried out the phytochemical and antibacterial activity of Knoxia sumatrensis (Retz.) Dc. using hexane and ethyl acetate extracts. Methods: The phytochemical screening was extracted from hexane and ethyl acetate solvent and its screening was analyzed by standard procedure. GC-MS technique was analyzed in ethyl acetate extract to identify the components present in the extract. The hexane and ethyl acetate extract of the plant was tested for antibacterial activity against human pathogenic bacteria using disk diffusion method. Results: The phytochemical screening was revealed the presence of phenols, saponins, flavonoids, cardiac glycosides, steroids, and tannins. The GC-MS results showed that the presence of seven bioactive compounds in ethyl acetate extract. The major compounds were identified such as N-Hexadecanoic Acid (9.336), 2-Piperidinone, N-[4-Bromo-N-Butyl] - (37. 883). The ethyl acetate extract showed good antibacterial activity. The maximum zone of inhibition was noticed in S. aureus (8.25 mm) using ethyl acetate extracts at 50 μg. Conclusion: Thus, this study the information regarding the phytochemical constituents present in the both extract. Hence, it can be used for further therapeutic applications in the near future study.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus

2021 ◽  
Vol 9 (1) ◽  
pp. 48
Author(s):  
Afif Rifqie Maulana ◽  
Bawon Triatmoko ◽  
Mochammad Amrun Hidayat
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacterial Resistance ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
World Health ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Infection is one of the main causes of world health problems, especially in developing countries like Indonesia, one of which is an acute respiratory infection caused by bacteria such as Staphylococcus aureus. Therapeutics are used for the treatment of infections today with antibiotics. However, there are many cases of bacterial resistance to antibiotics that need to develop alternatives derived from plants. One of the plants that have antibacterial activity is the leaves of Waru Gunung (Hibiscus macrophyllus). The purpose of this study was to determine whether the ethanolic extract of H. macrophyllus leaves and their fractions have antibacterial activity against S. aureus. The method used in this antibacterial activity test is the disk diffusion method with a test concentration of 5%, 10%, 15%, 20%, and 25%. The results of this study indicate that all test samples have antibacterial activity except in the water fraction. The highest antibacterial activity was obtained in the ethyl acetate fraction which attracted semipolar compounds in the ethanolic extract of H. macrophyllus leaves. The antibacterial activity of the test samples in sequence from high to low includes ethyl acetate fraction, ethanolic extract, n-hexane fraction, and water fraction.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

2020 ◽  
Vol 8 (3) ◽  
pp. 177
Author(s):  
Achmad Syarifudin Noor ◽  
Bawon Triatmoko ◽  
Nuri Nuri
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction ◽  
Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

scholarly journals Phytochemical Investigation and Antimicrobial Activity of Hexane, Ethyl Acetate and Methanol Fractions from Stem Bark of Icacina Trichantha Oliv. (Icacinaceae)

2020 ◽  
Vol 7 (1) ◽  
pp. 7-12
Author(s):  
Ojah Emmanuel Onah ◽  
Kachi Jolly Babangida
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Economic Losses ◽  
Phytochemical Screening ◽  
E Coli ◽  
Micro Organisms

Background: Micro-organisms are responsible for the transmission of a large number of diseases. It is hard to comprehend the amount of diseases, deaths and economic losses caused by micro-organisms alone. Plants are good sources of eco-friendly and readily available antimicrobial agents. Purpose: The aim of this study was to evaluate the chemical constituents and antimicrobial characteristics of three fractions from ethnomedicinal Icacina trichantha. Oliv. (Icacinaceae).Methods: Methanol extract from Icacina trichantha. Oliv was obtained by maceration and fractionated successively using hexane, and ethyl acetate. The antimicrobial properties of Icacina trichantha. Oliv was assessed using agar cup diffusion method on MRSA, P. aeruginosa, S. typhi, C. krusei, S. dysenteriae, S. pyrogenes, E. coli, K. pneumoniae, C. albicans, and C. tropicalis. Phytochemical screening on fractions was also evaluated using standard methods.Results: Phytochemical screening on fractions revealed the presence of saponins, alkaloids, steroids, tannins, and glycosides. Agar diffusion assay on fractions showed growth inhibitory effect on all the organisms except P. aeruginosa, S. typhi, and C. tropicalis. The MIC revealed that n-hexane fraction was active against MRSA, S. pyrogenes, E. coli, K. pneumonia, C. albicans and C. krusei at 10 mg/mL while S. dysenteriae was active at 5 mg/mL. The ethyl acetate fraction was active against all the organisms at a concentration of 5 mg/mL except P.aeruginosa, S.typhi and C.tropicalis. Methanol fraction showed activity of 5 mg/mL against MRSA, S. pyrogenes, E. coli, S. dysenteriae, C. albicans and C. krusei except for K. pneumoniae with activity at 10 mg/mL. Minimum bactericidal concentration/fungicidal concentration MBC/MFC evaluated on the n-hexane fraction revealed that MRSA, S. pyrogenes, E. coli, K. pneumoniae, S. dysenteriae, C. albicans, and C. krusei were activeat 20 mg/mL, while the ethyl acetate fraction had MBC/MFC of 10 mg/mL against all the organisms except P. aeruginosa, S. typhi, C. tropicalis. Methanol extract had MBC/MFC of 10 mg/mL against MSRA, E.coli and S. dysenteriae whereas S. pyrogenes, K. pneumoniae, C. albicans and C. krusei had MBC/MFC at 20 mg/mL.Conclusion: Icacina trichantha. Oliv. contain constituents with concentration dependent antimicrobial properties based on type of organism. The plant could be useful in the prevention and treatment of multi-resistant disease causing microorganisms.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

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