scholarly journals STUDY OF ANTIMICROBIAL ACTIVITY OF SILVER-DOPED HYDROXYAPATITE

2019 ◽  
pp. 243-245
Author(s):  
HIBU WAHID ◽  
ANJUVAN SINGH
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Xrd Analysis ◽  
Chemical Precipitation ◽  
Silver Concentration ◽  
Precipitation Method ◽  
Zone Of Inhibition

Objective: Hydroxyapatite (Hap) is an important material used for implants because of its structural similarity with calcium phosphate of the teeth and bones. Hap is mostly used in the situations where load-bearing implants are needed, such as in dental and orthopedic applications. Dental plaque is caused due to the bacteria which induces secondary caries through microleakage between the restoration and the tooth structure. Methods: An attempt has been made to provide materials with antibacterial activity by incorporating an antibacterial agent into the chemical mixture (Hap). This study delineates the process of synthesizing silver-doped Hap (Ca10Agx(PO4)6(OH)2) by wet chemical precipitation method and by varying the silver concentration (0.3%–0.5%). The synthesized silver-doped Hap was further characterized using Fourier transform infrared spectroscopy and X-ray diffraction (XRD) analysis. The antimicrobial activities were tested against Gram-negative bacteria Escherichia coli. Results: The maximum zone of inhibition against Escherichia coli was observed when equal concentration of Hydroxyapatite and Silver were mixed together i.e XAg=0.5% Zone of inhibition helps to determine the antimicrobial activity of silver-doped Hap. An increase in the zone of inhibition indicates the least growth of microorganisms which was observed at a silver concentration XAg=0.5%. Conclusions: Silver-doped Hap was synthesized successfully using the chemical precipitation method and was characterized using Fourier transform infrared spectroscopy and XRD analysis. It can be concluded that silver-doped Hap can be an excellent substitute material used as dental implant device.

Detecting antimicrobial resistance in Escherichia coli using benchtop attenuated total reflectance-Fourier transform infrared spectroscopy and machine learning

The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6211-6219
Author(s):  
Hewa G. S. Wijesinghe ◽  
Dominic J. Hare ◽  
Ahmed Mohamed ◽  
Alok K. Shah ◽  
Patrick N. A. Harris ◽  
...  
Keyword(s):  
Machine Learning ◽  
Escherichia Coli ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Antimicrobial Resistance ◽  
Fourier Transform Infrared Spectroscopy ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
E Coli ◽  
Machine Learning Model

ATR–FTIR with a machine learning model predicts ESBL genotype of unknown E. coli strains with 86.5% AUC.

Identification of microorganisms by Fourier-transform infrared spectroscopy

2018 ◽  
pp. 50-57 ◽  
Author(s):  
A. Yu. Suntsova ◽  
R. R. Guliev ◽  
D. A. Popov ◽  
T. Yu. Vostrikova ◽  
D. V. Dubodelov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Klebsiella Pneumoniae ◽  
Fourier Transform Infrared Spectroscopy ◽  
Infrared Spectra ◽  
Fourier Transform Infrared ◽  
Clinical Isolates

The need for novel techniques of rapid identification of pathogenic microorganisms arises from the massive spread of drug-resistant nosocomial strains and the emergence of centers for biohazard control. Fourier-transform infrared spectroscopy is a promising alternative to mass spectrometry as it is cost-effective, fast and suitable for field use. The aim of this work was to propose an algorithm for the identification of microorganisms in pure cultures based on the analysis of their Fourier transform infrared spectra. The algorithm is based on the automated principal component analysis of infrared spectra. Unlike its analogues described in the literature, the algorithm is capable of identifying bacteria regardless of the culture medium or growth phase. The training sample included the most prevalent causative agents of infections and sepsis in humans: Staphylococcus aureus (n = 67), Enterococcus faecalis (n = 10), Enterococcus faecium (n = 10), Klebsiella pneumoniae (n = 10), Escherichia coli (n = 10), Serratia marcescens (n = 10), Enterobacter cloacae (n = 10), Acinetobacter baumannii (n = 10), Pseudomonas aeruginosa (n = 10), and Candida albicans (n = 10). The model we built successfully passed a series of blind tests involving clinical isolates of 10 methicillin-resistant (MRSA) and 10 methicillin-sensitive (MSSA) Staphylococcus aureus strains as well as pair mixes of these cultures with clinical isolates of Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae.

scholarly journals A Comprehensive Study on the Effect of ZnO and ZnAl2O4 Nanoparticles on the Mechanical Properties of Epoxy Coating: Tensile and Hardness

2021 ◽  
Vol 15 (5) ◽  
pp. 45
Author(s):  
Abeer Shmait ◽  
Nour El Ghouch ◽  
J. Al Boukhari ◽  
A. M. Abdel-Gaber ◽  
R. Awad
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Tensile Tests ◽  
Precipitation Method ◽  
Zno Nps ◽  
Co Precipitation

ZnO and ZnAl2O4 nanoparticles (NPs) were successfully prepared by the co-precipitation method and characterized by x-ray powder diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The prepared NPs were incorporated into epoxy (EP) coating with mass ratios 200  800 mg/kg of ZnO NPs/EP and ZnAl2O4 NPs /EP. The prepared coatings were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy, and their mechanical properties were investigated, at room temperature, after 5, 10, 15, and 20 days of preparation. Tensile tests showed an improvement in the tensile properties, with the best improvement in ultimate tensile strength (93.2%) for 800 mg/kg ZnAl2O4 NPs/EP coating after 15 days of preparation. The ZnO NPs/EP and ZnAl2O4 NPs/EP coatings exhibited noticeable sensitivity to the stretching rate. Vickers microhardness (Hv) investigations showed normal indentation size effect behavior for all the samples. The best improvement in Hv was attained after 5 days of preparation, for all coatings, with the best improvement (9.15%) for 700 mg/kg ZnO NPs/EP.

INVESTIGATIONS ON CHITOSAN/α-Fe O NANOCOMPOSITE FOR 2 3 EFFICIENT ANTIBACTERIAL ACTIVITY

2021 ◽  
pp. 67-69
Author(s):  
M. Senthilkumar ◽  
R. Pandimurugan ◽  
M. Muthuvinayagam
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Xrd Analysis ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Visible Spectroscopy ◽  
Visible Absorption ◽  
Xrd Pattern ◽  
Simple Chemical ◽  
Uv Visible

Chitosan capped with α-Fe O nanocomposite was prepared by simple chemical precipitation method. The synthesized 2 3 Chitosan/α-Fe O nanocomposite was characterised by FTIR, UV–visible spectroscopy and XRD analysis. These results 2 3 showed that α-Fe O nanoparticles were successfully grown on the surface of chitosan. The FTIR vibrational peaks conrm the blending between 2 3 Chitosan and α-Fe O nanoparticles. Chitosan/α-Fe O nanocomposite. 2 3 2 3 Ultraviolet-Visible absorption spectra explain the optical property of The sharp peaks in XRD pattern indicate the increase in crystallinity nature of Chitosan/α-Fe O nanocomposite. Finally, the prepared Chitosan/α- 2 3 Fe O nanocomposite was tested for antibacterial activity against Staphylococcus aureus and Escherichia coli.

Detection of Escherichia coli O157:H7 and Salmonella Typhimurium Using Filtration followed by Fourier-Transform Infrared Spectroscopy†

2006 ◽  
Vol 69 (8) ◽  
pp. 1777-1784 ◽  
Author(s):  
Y. BURGULA ◽  
D. KHALI ◽  
S. KIM ◽  
S. S. KRISHNAN ◽  
M. A. COUSIN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Salmonella Typhimurium ◽  
Fourier Transform Infrared Spectroscopy ◽  
Large Scale ◽  
Fourier Transform Infrared ◽  
Nondestructive Method ◽  
Escherichia Coli O157 ◽  
Infrared Microscope

Fourier-transform infrared spectroscopy has been successfully used as a nondestructive method for identifying, distinguishing, and classifying pathogens. In this study, a less time-consuming Fourier-transform infrared procedure was developed to identify Escherichia coli O157:H7 and Salmonella Typhimurium. Samples containing 109 CFU/ml were prepared in tryptic soy broth and then serially diluted (up to eight times) to obtain bacterial solutions of 109 to 10 CFU/ml. These dilutions were incubated at 37°C for 6 h, samples were filtered through a Metricel filter hourly (for 0 to 6 h), and spectra were obtained using a ZnSe contact attenuated total reflectance accessory on a Continuμm infrared microscope. Midinfrared spectra (4,000 to 700 cm−1)of Salmonella Typhimurium and E. coli O157:H7 were generated, and peak areas in the region of 1,589 to 1,493 cm−1 were used to detect the pathogens. Initially, detection limits were between 106 and 107 CFU/ml without preenrichment, and samples starting with 500 CFU/ml were detectable following incubation for 6 h, when counts reached at least 106 CFU/ml. Compared with results of previously published studies in which Fourier-transform infrared spectroscopy was used to identify select pathogens, this method is more rapid and less expensive for practical large-scale sample analysis.

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