scholarly journals Biological synthesis of AgNPs BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING THE TUBEROUS ROOT EXTRACT OF IPOMOEA BATATAS AND THEIR CHARACTERIZATIONS AND ANTIBACTERIAL ACTIVITY

2019 ◽  
pp. 300-303 ◽  
Author(s):  
NAVEEN CHANDRA JOSHI ◽  
JUHI CHHABRA
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Ipomoea Batatas ◽  
Tuberous Root ◽  
Biological Synthesis ◽  
Root Extract ◽  
X Ray ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
Uv Visible

Objectives: Tuberous root extract based synthesis of silver nanoparticles (AgNPs), characterizations using Fourier-transform infrared spectroscopy (FT-IR), UV-visible, powder X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) techniques, and antibacterial activity of AgNPs against Staphylococcus aureus, Staphylococcus mutans, Proteus vulgaris, and Escherichia coli. Methods: Root extract of Ipomoea batatas (sweet potato) was prepared by boiling of small cut pieces of root with double distilled water. Added root extract with silver nitrate solution and centrifuged and collect the pellets. After several washing and drying, AgNPs have been preserved for characterizations and antibacterial activity. Results: The synthesized AgNPs were well characterized by FT-IR, UV-visible, XRD, FESEM, and EDX methods, and significant zones of inhibition observed around the loaded AgNPs on the agar plates. The zones of inhibition have been achieved 36, 40, 46, and 32 mm for E. coli, P. vulgaris, S. mutans, and S. aureus. Conclusion: The characterisation methods such as UV-Visible, FTIR, Powder XRD, FESEM and EDX indicate an efficient formation of AgNPs using root extract of I. batatas. The biologically synthesized AgNPs are found good antibacterial agents.

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

scholarly journals Phyto-Extract-Mediated Synthesis of Silver Nanoparticles Using Aqueous Extract of Sanvitalia procumbens, and Characterization, Optimization and Photocatalytic Degradation of Azo Dyes Orange G and Direct Blue-15

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6144
Author(s):  
Madeeha Aslam ◽  
Fozia Fozia ◽  
Anadil Gul ◽  
Ijaz Ahmad ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Carboxylic Acid ◽  
Plant Extract ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Direct Blue ◽  
Orange G ◽  
Ft Ir ◽  
Uv Visible ◽  
Direct Blue 15

Green synthesis of silver nanoparticles (AgNPs) employing an aqueous plant extract has emerged as a viable eco-friendly method. The aim of the study was to synthesize AgNPs by using plant extract of Sanvitalia procumbens (creeping zinnia) in which the phytochemicals present in plant extract act as a stabilizing and reducing agent. For the stability of the synthesized AgNPs, different parameters like AgNO3 concentration, volume ratios of AgNO3, temperature, pH, and contact time were studied. Further, AgNPs were characterized by UV–visible spectroscopy, FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray Diffraction), SEM (Scanning Electron Microscopy), and EDX (Energy Dispersive X-ray Spectrometer) analysis. FT-IR analysis showed that the plant extract contained essential functional groups like O–H stretching of carboxylic acid, N–H stretching of secondary amides, and C–N stretching of aromatic amines, and C–O indicates the vibration of alcohol, ester, and carboxylic acid that facilitated in the green synthesis of AgNPs. The crystalline nature of synthesized AgNPs was confirmed by XRD, while the elemental composition of AgNPs was detected by energy dispersive X-ray analysis (EDX). SEM studies showed the mean particle diameter of silver nanoparticles. The synthesized AgNPs were used for photocatalytic degradation of Orange G and Direct blue-15 (OG and DB-15), which were analyzed by UV-visible spectroscopy. Maximum degradation percentage of OG and DB-15 azo dyes was observed, without any significant silver leaching, thereby signifying notable photocatalytic properties of AgNPs.

scholarly journals GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 9 (5) ◽  
pp. 192 ◽  
Author(s):  
Subbiah Murugesan ◽  
Sundaresan Bhuvaneswari ◽  
Vajiravelu Sivamurugan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Red Alga ◽  
Diffusion Method ◽  
Present System ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

scholarly journals Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Prakash Bhuyar ◽  
Mohd Hasbi Ab. Rahim ◽  
Sathyavathi Sundararaju ◽  
Rameshprabu Ramaraj ◽  
Gaanty Pragas Maniam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Marine Algae ◽  
Marine Alga ◽  
Ag Nps ◽  
X Ray ◽  
Uv Visible ◽  
Scanning Electron

Abstract Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its characterization were fulfilled by using UV-visible spectrophotometer, Fourier transform infrared spectroscopy, scanning electron microscopy and field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Results UV-visible absorption spectrum revealed that the formation of Ag nanoparticles was increased by the addition of marine algae and the spectral peak observed between a wavelength of ~ 420 nm and 445 nm. In addition, SEM and FESEM images examined the surface morphology and the size of the synthesized NPs was relatively uniform in size ~ 25–60 nm. Energy-dispersive X-ray spectroscopy analysis confirmed the purity of Ag NPs with atomic percentage of 48.34% Ag. The synthesized Ag NPs showed highly potent antibacterial activity. The Staphylococcus aureus and Pseudomonas aeruginosa were found to be more susceptible to silver nanoparticles by forming 15.17 ± 0.58 mm and 13.33 ± 0.76 mm of diameter of the inhibition zone, respectively. Conclusions The study suggested that marine alga Padina sp. could be an alternative source for the production of Ag nanoparticles and are efficient antimicrobial compounds against both gram-negative and gram-positive bacteria which can be a promising material against infectious bacteria.

Antibacterial Activity of Silver Nanoparticles Using Jasminum auriculatum Stem Extract

2019 ◽  
Vol 18 (01) ◽  
pp. 1850011 ◽  
Author(s):  
S. Balasubramanian ◽  
U. Jeyapaul ◽  
S. Mary Jelastin Kala
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Disc Diffusion Method ◽  
Stem Extract ◽  
Ft Ir ◽  
Uv Visible ◽  
Size And Morphology ◽  
Green Synthesized Silver Nanoparticles

In the present study, bio-meditated silver nanoparticles have been synthesized using Jasminum auriculatum stem extracts. The silver nanoparticles were confirmed using UV-visible and FT-IR spectra. The size and morphology of silver nanoparticles were confirmed using XRD, SEM and EDAX techniques. The antibacterial activity of green synthesized silver nanoparticles was assessed by disc diffusion method against human pathogens. The antibacterial studies indicate that green synthesized silver nanoparticles have several pharmaceutical applications for the management of deadly human pathogens.

scholarly journals In Vitro Antibacterial Activity and Mechanism of Silver Nanoparticles against Foodborne Pathogens

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
S. Rajeshkumar ◽  
C. Malarkodi
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Food Safety ◽  
Pathogenic Bacteria ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
X Ray ◽  
Food Borne ◽  
Uv Visible

Biosynthesis of silver nanoparticles usingPlanomicrobiumsp. and to explore the antibacterial activity against food borne pathogenic bacteriaBacillus subtilis, (3053)Klebsiella planticola(2727)Klebsiella pneumoniae(MAA)Serratia nematodiphila(CAA) andEscherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL ofPlanomicrobiumsp. culture supernatant. The bioreduction of pure AgNO3was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

scholarly journals Phytochemical mediated synthesis of silver nanoparticles and their antibacterial activity

2021 ◽  
Vol 3 (6) ◽  
Author(s):  
Lakshman Kumar Dogiparthi ◽  
Siva Sankar Sana ◽  
Sumaya Zikreen Shaik ◽  
Maneesha Reddy Kalvapalli ◽  
Gayathri Kurupati ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Visible Spectrum ◽  
Plant Metabolites ◽  
X Ray ◽  
Aqueous Leaf Extract ◽  
Ft Ir ◽  
Uv Visible ◽  
Face Centered

AbstractIn this present work, we described a bio-reduction method for the generation of silver nanoparticles (AgNPs) using aqueous leaf extract of Micrargeria wightii (M. wightii), which is a gifted alternative to other physicochemical routes. The prepared AgNPs were characterized by UV–visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (X-RD), Transmission Electron Microscopy (TEM) with EDX and Dynamic light scattering (DLS). UV–visible spectrum showed a characteristic absorption peak at 440 nm of synthesized AgNPs. FT-IR analysis confirmed the existence of plant metabolites, which are responsible for the reduction of Ag (I) ions into Ag (0) NPs. X-RD pattern studies confirm the presence of the pure face-centered cubiccrystalline nature of Ag. Energy-dispersive X-ray (E-DX) spectrum showed the elemental composition of synthesized nanoparticles. Furthermore, TEM images confirm the formation of spherical shaped nano-silver particles with sizes ranging from 30 to 70 nm and supported by particle size analyzer, Dynamic Light Scattering (DLS). Thus, the present investigation provides an easy, eco-friendly and straightforward route for the synthesis of the antibacterial agent against Bacillus subtilis subtilis and Pseudomonas aeruginosa, with 15 and 13 mm zone of inhibition (ZOI) respectively.

Green synthesis of silver nanoparticles of olea ferruginea root extract: Characterizations, biological assays and their catalytic application in dye degradation

2021 ◽  
pp. 1-13
Author(s):  
Iqbal Wadan ◽  
Haroon Khan ◽  
Kamran Tahir ◽  
Muhammad Khalid Khan ◽  
Barkat Ali Khan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Dye Degradation ◽  
Root Extract ◽  
X Ray Diffraction ◽  
Visible Absorption ◽  
Biological Assays ◽  
X Ray ◽  
Transmission Electron ◽  
Uv Visible

In current work, silver nanoparticles (AgNPs) were prepared by a rapid biogenic technique “Green method” utilizing root extract of Olea ferruginea. The synthesized nanoparticles were characterized for UV visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning electron microscopy (SEM), High Resolution Transmission Electron Microscopy Studies (HRTEM) and biological assays. UV visible absorption spectroscopy confirms the formation of AgNPs by giving Surface Plasmon Resonance (SPR) peak at 430 nm. FTIR study showed occurrence of various functional groups present in root extract of Olea ferruginea. X-ray diffraction analysis determined the crystalline nature of bio-fabricated silver nanoparticles. The elemental composition of green made silver nanoparticles was studied through Energy Dispersive Spectroscopy (EDS) analysis. SEM & HRTEM study revealed the size, shape, surface morphology & dispersion level of molecules. The biologically synthesized AgNPs showed high antimicrobial, antifungal and antioxidant activity. The AgNPs are observed to be an excellent catalyst on reduction of hazardous dyes, which is confirmed by a decrease in absorbance of maximum values.

scholarly journals Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Md. Abdullah Al Masud ◽  
Hamid Shaikh ◽  
Md. Shamsul Alam ◽  
M. Minnatul Karim ◽  
M. Abdul Momin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bombyx Mori ◽  
Multidrug Resistant ◽  
Visible Spectroscopy ◽  
Silk Sericin ◽  
X Ray ◽  
Synthesis Strategy ◽  
Uv Visible ◽  
Multidrug Resistant Pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

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