ANTI-MELANOMA BIO-EFFICACY OF THE PLANT MADHUCA LONGIFOLIA AND ITS ENHANCEMENT USING BIOACTIVE PRINCIPLE LOADED GOLD NANOPARTICLE

Objective: Unexplored in-vivo anti-melanoma bio-efficacy of the plant Madhuca longifolia (bark) has been carried out against C57BL/6 mice. Methods: Optimized experimental conditions of phytofabrication of gold nanoparticles were as follows: flavonoid content (1 ml, 0.5 mg/ml), sodium tetrachloroaurate dihydrate solution (2 ml, 1 mM), and sonication (15 min, 20 KHz) at pH 4. The optical properties; ultraviolet-visible spectrophotometer (UV-Vis), particles size and zeta potential (Zetasizer), miller indices; X-ray diffraction (XRD), morphology; field emission-scanning electron microscope (FE-SEM), particle size; high resolution-transmission electron microscopy (HR-TEM), surface roughness; atomic force microscopy (AFM) and elemental composition; and energy dispersive X-rays (EDX) of flavonoid loaded gold nanoparticles. In-vivo anti-melanoma bio-efficacy has been carried out against C57BL mice. Radioisotopic, hematological, and histopathological studies were carried out using standard procedures. Results: Redox potential of the total flavonoid extracted from the bark of the plant (Madhuca longifolia) has been used for the fabrication of flavonoid loaded gold nanoparticles (F@AuNp) and confirmed for the first time their significant anti-melanoma bio-efficacy. The finding is supported by hematological and histopathological studies carried out in the organs (liver, kidney, and intestine) of C57BL mice. The significant enhancement in phytofabricated F@AuNp compared to native bark extract of the plant has been assigned to enhanced stay period and nanosizing, biocompatibility, nontoxic nature, and enhanced beneficial payload to the cancerous cells. Conclusion: Such phytofabricated gold nanoparticles possess an admirable prospect for the expansion of herbal nanomedicine for anti-melanoma bio-efficacy.

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Radiosensitization of Prostate Cancers In Vitro and In Vivo to Erbium-filtered Orthovoltage X-rays Using Actively Targeted Gold Nanoparticles

Scientific Reports ◽

10.1038/s41598-017-18304-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 12

Author(s):

Allison M. Khoo ◽

Sang Hyun Cho ◽

Francisco J. Reynoso ◽

Maureen Aliru ◽

Kathryn Aziz ◽

...

Keyword(s):

Gold Nanoparticles ◽

X Rays ◽

Prostate Cancers

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5-Phenyl-10,15,20-Tris(4-sulfonatophenyl)porphyrin: Synthesis, Catalysis, and Structural Studies

Molecules ◽

10.3390/molecules23123363 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3363 ◽

Cited By ~ 3

Author(s):

Aitor Arlegui ◽

Zoubir El-Hachemi ◽

Joaquim Crusats ◽

Albert Moyano

Keyword(s):

Alder Reaction ◽

Water Soluble ◽

Experimental Conditions ◽

One Pot ◽

Force Microscopy ◽

Diels Alder Reaction ◽

Transmission Electron ◽

Porphyrin Synthesis ◽

The One ◽

Water Soluble Porphyrin

A convenient protocol for the preparation of 5-phenyl-10,15,20-tris(4-sulfonatophenyl)porphyrin, a water-soluble porphyrin with unique aggregation properties, is described. The procedure relies on the one-pot reductive deamination of 5-(4-aminophenyl)-10,15,20-tris(4-sulfonatophenyl)porphyrin, that can be in turn easily obtained from 5,10,15,20-tetraphenylporphyrin by a known three-step sequence involving mononitration, nitro to amine reduction and sulfonation of the phenyl groups. This method provides the title porphyrin in gram scale, and compares very favorably with the up to now only described procedure based on the partial sulfonation of TPP, that involves a long and tedious chromatographic enrichment of the final compound. This has allowed us to study for the first time both the use of its zwitterionic aggregate as a supramolecular catalyst of the aqueous Diels–Alder reaction, and the morphology of the aggregates obtained under optimized experimental conditions by atomic force microscopy and also by transmission electron cryomicroscopy.

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Green Nanoparticles for Mosquito Control

The Scientific World JOURNAL ◽

10.1155/2014/496362 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 27

Author(s):

Namita Soni ◽

Soam Prakash

Keyword(s):

Gold Nanoparticles ◽

Mosquito Control ◽

Spherical Shape ◽

Probit Analysis ◽

Bark Extract ◽

Green Method ◽

Silver And Gold Nanoparticles ◽

Transmission Electron ◽

Uv Visible ◽

Filariasis Vector

Here, we have used the green method for synthesis of silver and gold nanoparticles. In the present study the silver (Ag) and gold (Au) nanoparticles (NPs) were synthesized by using the aqueous bark extract of Indian spice dalchini (Cinnamomum zeylanicum) (C. zyelanicumorC. verumJ. Presl). Additionally, we have used these synthesized nanoparticles for mosquito control. The larvicidal activity has been tested against the malaria vectorAnopheles stephensiand filariasis vectorCulex quinquefasciatus. The results were obtained using UV-visible spectrophotometer and the images were recorded with a transmission electron microscope (TEM). The efficacy tests were then performed at different concentrations and varying numbers of hours by probit analysis. The synthesized AgNPs were in spherical shape and average sizes (11.77 nm AgNPs and 46.48 nm AuNPs). The larvae ofAn. stephensiwere found highly susceptible to the synthesized AgNPs and AuNPs than theCx. quinquefasciatus. These results suggest that theC. zeylanicumsynthesized silver and gold nanoparticles have the potential to be used as an ideal ecofriendly approach for the control of mosquito.

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Antitumour and Toxicity Evaluation of a Ru(II)-Cyclopentadienyl Complex in a Prostate Cancer Model by Imaging Tools

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520619666190318152726 ◽

2019 ◽

Vol 19 (10) ◽

pp. 1262-1275 ◽

Cited By ~ 2

Author(s):

Lurdes Gano ◽

Teresa Pinheiro ◽

António P. Matos ◽

Francisco Tortosa ◽

Tiago F. Jorge ◽

...

Keyword(s):

Antitumour Activity ◽

Renal Plasma Flow ◽

Mice Model ◽

Experimental Conditions ◽

Anticancer Properties ◽

Transmission Electron ◽

Cyclopentadienyl Complexes ◽

Tumour Reduction

Background: Ruthenium complexes have been extensively investigated for their prospective value as alternatives to cisplatin. Recently, we reported the in vitro anticancer properties of a family of organometallic ruthenium( II)-cyclopentadienyl complexes and have explored their mechanism of action. Objective: The purpose of this study was to evaluate the in vivo antitumour efficacy and toxicity of one of these Ru(II) compounds, [RuCp(mTPPMSNa)(2,2′-bipy)][CF3SO2] (TM85) which displayed an interesting spectrum of activity against several cancer cells. Methods: Studies to assess the antitumour activity and toxicity were performed in a metastatic prostate (PC3) mice model using ICP-MS, nuclear microscopy, elemental analysis and Transmission Electron Microscopy (TEM). Results: TM85 showed low systemic toxicity but no significant tumour reduction, when administered at tolerated dose (20mg/kg) over 10 days. Ru was mainly retained in the liver and less in kidneys, with low accumulation in tumour. Increased bilirubin levels, anomalous Ca and Fe concentrations in liver and mitochondria alterations were indicative of liver injury. The hepatotoxicity observed was less severe than that of cisplatin and no nephrotoxicity was found. Conclusion: Under the experimental conditions of this study, TM85 is less toxic than cisplatin, induces similar tumour reduction and avoids the formation of metastatic foci. No renal toxicity was observed by the analysis of creatinine levels and the effective renal plasma flow by 99mTc-MAG3 clearance. Hence, it can be considered a valuable compound for further studies in the field of Ru-based anticancer drugs.

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An Organic Acid-induced Synthesis and Characterization of Selenium Nanoparticles

Journal of Nanotechnology ◽

10.1155/2011/651971 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 36

Author(s):

Charu Dwivedi ◽

Chetan P. Shah ◽

Krishankant Singh ◽

Manmohan Kumar ◽

Parma Nand Bajaj

Keyword(s):

Aqueous Medium ◽

High Speed ◽

Selenium Nanoparticles ◽

Optical Absorption Spectroscopy ◽

X Rays ◽

Ambient Conditions ◽

Scanning Calorimetry ◽

Force Microscopy ◽

Transmission Electron ◽

Microscopy Techniques

A simple wet chemical method has been developed to synthesize selenium nanoparticles (size 40–100 nm), by the reaction of sodium selenosulphate precursor with different organic acids in aqueous medium, under ambient conditions. Polyvinyl alcohol has been used to stabilize the selenium nanoparticles. The synthesized nanoparticles can be separated from its sol by using a high-speed centrifuge and can be redispersed in aqueous medium with a sonicator. UV-visible optical absorption spectroscopy, X-ray diffraction, energy dispersive X-rays, differential scanning calorimetry, atomic force microscopy, and transmission electron microscopy techniques have been employed to characterize the synthesized selenium nanoparticles.

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Nanoparticle Assembly via Hydrogen-Bonding: IRS, TEM and AFM Characterizations

MRS Proceedings ◽

10.1557/proc-635-c4.5 ◽

2001 ◽

Vol 635 ◽

Cited By ~ 1

Author(s):

Li Han ◽

Mathew M. Maye ◽

Chuan-Jian Zhong

Keyword(s):

Gold Nanoparticles ◽

Hydrogen Bonding ◽

Morphological Properties ◽

Nanoparticle Assembly ◽

Force Microscopy ◽

Atomic Force ◽

Transmission Electron ◽

Infrared Reflection ◽

Afm Imaging ◽

Infrared Reflection Spectroscopy

AbstractThis paper reports results of the characterizations of nanoparticle assembly formed via spontaneous core-shell and shell-shell reactivities at thiolate-capped gold nanoparticles. Gold nanoparticles of two different core sizes and thiols with carboxylic acid terminals are exploited as a model system. The reactivities involve covalent Au-thiolate bonding and non-covalent hydrogen-bonding with anisotropic linking character. We employed infrared reflection spectroscopy (IRS), atomic force microscopy (AFM) and transmission electron microscopy (TEM) for the characterizations. While IRS provides structural assessment, TEM and AFM imaging measurements probe the morphological properties.

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Caffeic Acid: Potential Applications in Nanotechnology as a Green Reducing Agent for Sustainable Synthesis of Gold Nanoparticles

Natural Product Communications ◽

10.1177/1934578x1501000424 ◽

2015 ◽

Vol 10 (4) ◽

pp. 1934578X1501000

Author(s):

Yu Seon Seo ◽

Song-Hyun Cha ◽

Seonho Cho ◽

Hye-Ran Yoon ◽

Young-Hwa Kang ◽

...

Keyword(s):

Gold Nanoparticles ◽

Caffeic Acid ◽

Average Diameter ◽

Reducing Agent ◽

Force Microscopy ◽

Atomic Force ◽

Transmission Electron ◽

Potential Applications ◽

Acid Potential ◽

Sustainability Initiatives

The sustainable synthesis of gold nanoparticles from gold ions was conducted with caffeic acid as a green reducing agent. The formation of gold nanoparticles was confirmed by spectroscopic and microscopic methods. Spherical nanoparticles with an average diameter of 29.99 ± 7.43 nm were observed in high-resolution transmission electron microscopy and atomic force microscopy images. The newly prepared gold nanoparticles exhibited catalytic activity toward the reduction of 4-nitrophenol to 4-aminophenol in the presence of sodium borohydride. This system enables the preparation of green catalysts using plant natural products as reducing agents, which fulfills the growing need for sustainability initiatives.

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Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

Crystals ◽

10.3390/cryst9120612 ◽

2019 ◽

Vol 9 (12) ◽

pp. 612 ◽

Cited By ~ 2

Author(s):

Nancy Tepale ◽

Víctor V. A. Fernández-Escamilla ◽

Clara Carreon-Alvarez ◽

Valeria J. González-Coronel ◽

Adan Luna-Flores ◽

...

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Photoelectron Spectroscopy ◽

X Ray Diffraction ◽

Fundamental Study ◽

X Ray ◽

Force Microscopy ◽

X Ray Scattering ◽

Atomic Force ◽

Transmission Electron

The fundamental aspects of the manufacturing of gold nanoparticles (AuNPs) are discussed in this review. In particular, attention is devoted to the development of a simple and versatile method for the preparation of these nanoparticles. Eco-friendly synthetic routes, such as wet chemistry and biosynthesis with the aid of polymers, are of particular interest. Polymers can act as reducing and/or capping agents, or as soft templates leading to hybrid nanomaterials. This methodology allows control of the synthesis and stability of nanomaterials with novel properties. Thus, this review focus on a fundamental study of AuNPs properties and different techniques to characterize them, e.g., Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), UV-Visible spectroscopy, Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy, Small-angle X-Ray Scattering (SAXS), and rheology. Recently, AuNPs obtained by “green” synthesis have been applied in catalysis, in medicine, and as antibacterials, sensors, among others.

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Enzyme-Free Electrochemical Nano-Immunosensor Based on Graphene Quantum Dots and Gold Nanoparticles for Cardiac Biomarker Determination

Nanomaterials ◽

10.3390/nano11030578 ◽

2021 ◽

Vol 11 (3) ◽

pp. 578

Author(s):

Bhargav D. Mansuriya ◽

Zeynep Altintas

Keyword(s):

Electron Microscopy ◽

Quantum Dots ◽

Gold Nanoparticles ◽

Troponin I ◽

Electrochemical Techniques ◽

Graphene Quantum Dots ◽

High Specificity ◽

Force Microscopy ◽

Atomic Force ◽

Transmission Electron

An ultrasensitive enzyme-free electrochemical nano-immunosensor based on a screen-printed gold electrode (SPGE) modified with graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) was engineered to detect cardiac troponin-I (cTnI) for the early diagnosis of acute myocardial infarction (AMI). The GQDs and in-house synthesized AuNPs were implanted onto the SPGE and allowed for anti-cTnI immobilization prior to quantifying cTnI. The biomarker could be determined in a wide concentration range using square-wave voltammetry (SWV), cyclic voltammetry (CV), electron impedance spectroscopy (EIS) and amperometry. The analyses were performed in buffer, as well as in human serum, in the investigation ranges of 1–1000 and 10–1000 pg mL−1, respectively. The detection time ranged from 10.5–13 min, depending on the electrochemical method employed. The detection limit was calculated as 0.1 and 0.5 pg mL−1 for buffer and serum, respectively. The sensitivity of the immunosensor was found to be 6.81 µA cm−2 pg mL−1, whereas the binding affinity was determined to be <0.89 pM. The sensor showed high specificity for cTnI with slight responses for nonspecific biomolecules. Each step of the sensor fabrication was characterized using CV, SWV, EIS and atomic force microscopy (AFM). Moreover, AuNPs, GQDs and their nanocomposites were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This is the first immunosensor that represents the successful determination of an analyte using four different electrochemical techniques. Such a sensor could demonstrate a promising future for on-site detection of AMI with its sensitivity, cost-effectiveness, rapidity and specificity.

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POLYISOBUTYLENE-BASED THERMOPLASTIC BIORUBBERS

Rubber Chemistry and Technology ◽

10.5254/1.3525683 ◽

2010 ◽

Vol 83 (3) ◽

pp. 235-246 ◽

Cited By ~ 9

Author(s):

Judit E. Puskas ◽

Lucas M. Dos Santos ◽

Elizabeth Orlowski

Keyword(s):

Thermoplastic Elastomers ◽

Surface Topology ◽

Drug Eluting Stent ◽

Water Contact ◽

Neat Polymer ◽

Force Microscopy ◽

Self Assembling ◽

Transmission Electron ◽

New Generation

Abstract Linear triblock poly(styrene-b-isobutylene-b-styrene) (SIBS), the first representative of polyisobutylene (PIB)-based biomaterials, is approved by the Food and Drug Administration for use in the Taxus® Drug Eluting stent. SIBS and the new generation of dendritic or arborescent D_SIBS are self-assembling thermoplastic elastomers (TPEs), or “biorubbers.” D_SIBS has lower creep and improved fatigue life. We recently produced composites of novel D_PIB-based TPEs with carbon and silica. These composites had 2–10 times higher tensile strength than that of the neat polymer. The composite with 37.5 wt. % carbon black was nonconductive, demonstrating excellent filler dispersion. Transmission electron microscopy and atomic force microscopy analysis supported the formation of a nanocomposite with nanosized surface topology. The water contact angle of the biorubbers was significantly lower than that of silicone rubber. The carbon nanocomposite showed excellent biocompatibility in vivo, having thinner capsules than silicone after 180 days implantation into rabbits. Bone compatibility was also excellent. The improved biocompatibility was most likely due to a combination of hydrophilicity and surface nanotopology. Fundamental studies of the effect of surface properties of these biorubbers on biocompatibility are ongoing in our laboratory.

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