THERAPEUTIC EVALUATION OF CHEMICALLY SYNTHESIZED COPPER NANOPARTICLES TO PROMOTE FULL-THICKNESS EXCISIONAL WOUND HEALING

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020v12i6.38869 ◽

2020 ◽

pp. 136-142

Author(s):

ASHISH KUMAR ◽

VINAY PANDIT ◽

UPENDRA NAGAICH

Keyword(s):

Wound Healing ◽

Copper Nanoparticles ◽

Chemical Properties ◽

Single Step ◽

Full Thickness ◽

Environment Friendly ◽

Healing Property ◽

Excisional Wound ◽

Anti Inflammatory

Objective: The purpose of this research was, synthesis of copper nanoparticles using environment friendly cementation method and evaluate their wound healing property on full-thickness excisional wound. Methods: Present study reports the synthesis of CNPs by single-step cementation method. Evaluation of CNPs was endorsed by morphological and chemical properties. Furthermore, CNPs was evaluated for its antibacterial potential and invitro hemocompatibility. Additionally, pharmacological evaluation of CNPs was assessed against excisional wound. Results: Characterization of final product indicate, particle size of CNPs were ranging from 100-150 nm. CNPs showed significant antibacterial activity (A= 2.1±0.1 mm, B =2.1±0.1 mm, C = 1.9±0.2 mm, at 10µg/ml), along with superior hemocompatibility (RBC cell survival 97±1 %). Further CNPs formulation shows increased level of anti-inflammatory cytokinin’s (IL-10, 42.7%) as compared to standard (STD), vehicle control, and normal control groups, attributed to accelerated wound healing (p<0.05 vs STD). Conclusion: The consequences the present investigation endorse the accelerated wound healing potential of CNPs with its anti-inflammatory potential.

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The Efficacy of Gelam Honey Dressing towards Excisional Wound Healing

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/805932 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 15

Author(s):

Mui Koon Tan ◽

Durriyyah Sharifah Hasan Adli ◽

Mohd Amzari Tumiran ◽

Mahmood Ameen Abdulla ◽

Kamaruddin Mohd Yusoff

Keyword(s):

Wound Healing ◽

Saline Group ◽

Wound Management ◽

Full Thickness ◽

Sprague Dawley ◽

Histological Features ◽

Positive Effects ◽

Gelam Honey ◽

Neck Area ◽

Excisional Wound

Honey is one of the oldest substances used in wound management. Efficacy of Gelam honey in wound healing was evaluated in this paper.Sprague-Dawleyrats were randomly divided into four groups of 24 rats each (untreated group, saline group, Intrasite Gel group, and Gelam honey group) with 2 cm by 2 cm full thickness, excisional wound created on neck area. Wounds were dressed topically according to groups. Rats were sacrificed on days 1, 5, 10, and 15 of treatments. Wounds were then processed for macroscopic and histological observations. Gelam-honey-dressed wounds healed earlier (day 13) than untreated and saline treated groups, as did wounds treated with Intrasite Gel. Honey-treated wounds exhibited less scab and only thin scar formations. Histological features demonstrated positive effects of Gelam honey on the wounds. This paper showed that Gelam honey dressing on excisional wound accelerated the process of wound healing.

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PREPARATION AND EVALUATION OF COPPER NANOPARTICLES LOADED HYDROGEL FOR BURNS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021v13i2.40558 ◽

2021 ◽

pp. 180-189

Author(s):

ASHISH KUMAR ◽

VINAY PANDIT ◽

UPENDRA NAGAICH

Keyword(s):

Wound Healing ◽

Inflammatory Cytokines ◽

Copper Nanoparticles ◽

Wound Closure ◽

Hydroxypropyl Methylcellulose ◽

Influential Factors ◽

Factor Screening ◽

Closure Rate ◽

Screening Study ◽

Anti Inflammatory

Objective: The present study focuses on the development and optimization of copper nanoparticles (CNPs) loaded hydrogel for the treatment of dermal burn injuries. Methods: CNPs gel was prepared by dispersing the variable concentration of polyvinylpyrrolidone (PVP K30) and hydroxypropyl methylcellulose (HPMC) in distilled water, PEG 400, and copper nanoparticles. factor screening study was performed for identification of influential factors, followed by optimization study using three-factor Box-Behnken design. Results: Optimized nanogel formulation, when compared to normal control (NC), shows a significant reduction of pro-inflammatory cytokines (IL-6 = 39.74 % and TNF-α =49.37%) and increased level of anti-inflammatory cytokines (IL-10 = 30.90%), indicating reduced inflammation. Further, the wound closure rate of CNPs gel shows significant (12.27 %) wound closure as compared to the NC group and complete wound closure (100 %) on the 14th day, indicating accelerated wound healing. Conclusion: the present investigation endorses accelerated scar-free, accelerated wound healing potential of copper nanoparticles gel with anti-inflammatory potential.

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A Review on Synthesis, Characterization and Applications of Copper Nanoparticles Using Green Method

NANO ◽

10.1142/s1793292017500436 ◽

2017 ◽

Vol 12 (04) ◽

pp. 1750043 ◽

Cited By ~ 24

Author(s):

Muhammad Rafique ◽

Ahson J. Shaikh ◽

Reena Rasheed ◽

Muhammad Bilal Tahir ◽

Hafiz Faiq Bakhat ◽

...

Keyword(s):

Copper Nanoparticles ◽

Inorganic Material ◽

Synthesis Method ◽

Green Method ◽

Environment Friendly ◽

Modern Age ◽

Wide Range ◽

Material Sciences ◽

Conventional Methods

To address accosts of this modern age, the synthesis of metal nanoparticles is more important than ever. Copper has been recognized as a nontoxic, safe inorganic material, cheaper antibacterial/antifungal agent, and has high potential in a wide range of biological, catalytic and sensors applications more particularly in the form of nanoparticles. This resulted in the development of numerous methods for the synthesis of copper nanoparticles. As conventional methods like chemical and physical methods have several limitations so there is need to an alternate method. Due to nontoxic and eco-friendly nature, it has recently been shifted toward green synthesis of copper nanoparticles over conventional methods. Additionally, characterization of the synthesized nanoparticles is essential for their use in various applications. This review gives an overview of environment friendly synthesis method of copper nanoparticles and their applications on the basis of their potential selectivity and preferences in a number of fields like material sciences and biomedicine.

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Copper nanoparticles promote rapid wound healing in acute full thickness defect via acceleration of skin cell migration, proliferation, and neovascularization

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2019.07.110 ◽

2019 ◽

Vol 517 (4) ◽

pp. 684-690 ◽

Cited By ~ 7

Author(s):

Sanaz Alizadeh ◽

Bagher Seyedalipour ◽

Saeed Shafieyan ◽

Abolfazl Kheime ◽

Parvaneh Mohammadi ◽

...

Keyword(s):

Wound Healing ◽

Cell Migration ◽

Copper Nanoparticles ◽

Full Thickness ◽

Skin Cell

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Antimicrobial and anti-inflammatory activities, wound-healing effectiveness and chemical characterization of the latex of Jatropha neopauciflora Pax

Journal of Ethnopharmacology ◽

10.1016/j.jep.2017.04.003 ◽

2017 ◽

Vol 204 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

A.B. Hernandez-Hernandez ◽

F.J. Alarcon-Aguilar ◽

J.C. Almanza-Perez ◽

O. Nieto-Yañez ◽

J.M. Olivares-Sanchez ◽

...

Keyword(s):

Wound Healing ◽

Chemical Characterization ◽

Anti Inflammatory

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In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

Molecules ◽

10.3390/molecules25112695 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2695

Author(s):

Mehmet Evren Okur ◽

Ayşe Esra Karadağ ◽

Neslihan Üstündağ Okur ◽

Yağmur Özhan ◽

Hande Sipahi ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Activity ◽

Wound Model ◽

Aerial Parts ◽

Excisional Wound ◽

Anti Inflammatory ◽

Cell Culture Assay ◽

Gel Formulations

The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH• assay showed IC50 = 0.89 mg/mL, and the ABTS• assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC–FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

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