scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY ETHANOLIC EXTRACTS OF DRIED FRUITS OF SOLANUM TORVUM (FAMILY-SOLANACEAE)

Author(s):  
Lawrence Kalita ◽  
Biswajit Dash ◽  
Uttam Borah ◽  
Juman Deka ◽  
Suvakanta Dash

Objective: To study the antimicrobial activity ethanolic extracts of dried fruits of Solanumtorvum (family-solanaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of dried fruits of the herb Solanumtorvum (family-solanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts were examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of dried fruits of the plant Solanumtorvum (family-solanaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: It can be concluded ethanolic extract of whole aerial parts of the Solanumtorvum (family-solanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

Author(s):  
Uttam Borah ◽  
Biswajit Dash ◽  
Suvakanta Dash ◽  
Lawrence Kalita

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.


Author(s):  
Imdadul Haque Chaudhury ◽  
Trishna Das ◽  
Dipankar Saha

Objective: To evaluate the antimicrobial activity ethanolic extracts of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of stems of the plant Ipomoea aquatic Forsk. (Family-Convolvulaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: The present study concluded that ethanolic extract of stems of the plant Ipomoea aquatica Forsk. Contain the high presence of phytochemical. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 


Author(s):  
Junab Ali ◽  
Biswajit Das ◽  
Trideep Saikia

Objective: To evaluate the antimicrobial activity of methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) in conjugation with phytochemical analysis.Methods: The methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) was separated from fruits, shade dried, powdered and extracted using methanol, analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is Gram-positive (Staphylococcus aureus) and other is Gram-negative (Escherichia coli) and 1 fungal strains (Candida albicans) using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the methanolic extract of the fruits peel Citrus limon. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides and citric acids and tannins. The antimicrobial activity of the methanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that methanolic extract of the peel of Citrus Limon contains the high presence of phytochemicals. The methanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 


2015 ◽  
Vol 1 (4) ◽  
pp. 29-34
Author(s):  
Dharma Prasad Khanal ◽  
B Raut ◽  
K S Dangol

Herbal medicines possess a great demand in both developed and developing countries as a source of primary health care owing to their attributes having wide biological and medicinal activities, high safety margins and lesser costs. People living in rural areas of Nepal depend largely in the herbal medicines for the treatment. The present study concerns with the study of pharmacognostic characteristics, phytochemical constituents and the biological activity of Amaranthus spinosus L. The hexane, chloroform, ethanolic and aqueous extract of its aerial parts were subjected to preliminary phytochemical analysis and detected saponin, carbohydrate, tannin, protein, glycoside, flavonoid and phenol as phytoconstituents. The transverse section of the stem and leaves showed characteristic vascular bundle tissues. All the extracts were used for testing the antimicrobial activity and brine shrimp lethality. No extract showed the antimicrobial activity and ethanolic extract showed LC50 value of 31.62 ppm. Hexane, chloroform and water extracts were less toxic with LC50 values 236.88 ppm,194.98 ppm and 320.71 ppm respectively.DOI: http://dx.doi.org/10.3126/jmmihs.v1i4.11999Journal of Manmohan Memorial Institute of Health Sciences Vol. 1, Issue 4, 2015Page: 29-34


Author(s):  
Jafar Ali ◽  
Dibyendu Shil

Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.


Author(s):  
Abdul Samim ◽  
Sumit Das

Objective: To estimate the anti-microbial activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared and studied for phytochemical constituents by using various standard methods. The antimicrobial activity of plant extract was performed on two bacterial strains and one fungal strain using disc diffusion method.Results: The present study shows the phytochemical analysis, antimicrobial activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol, terpenoids, glycosides, saponins respectively. The anti-microbial activity of the plant extract showed significant results against all three of the test organisms.Conclusion: The present study concluded that the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis (night flowering jasmine) contains the highly presence of Phytochemical constituents. The hydro-alcoholic and petroleum ether extract of the plant was found to possess promising antimicrobial activity when compared with the standards.


Author(s):  
Sujata Paul ◽  
Biswajit Dash ◽  
Aditya Jyoti Bora ◽  
Bikash Gupta

Objective: To evaluate the anti-microbial activity of ethanolic extract of fruits of Annona reticulata (family-Annonaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of fruits of Annona reticulata (family-Annonaceae) was prepared by Soxhlet extraction and analysed for phytochemical constituents using standard methods. The anti-microbial activity of the plant extract was examined against bacterial strains and fungal strains using disc diffusion method.Results: The present investigation shows the phytochemical analysis, anti-microbial activity of the ethanolic extract of the plant Annona reticulata. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, steroids, proteins and amino acids and tannins. The anti-microbial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of fruits of the Annona reticulata contain high presence of phytochemicals. The ethanolic extract of the plant was found to possess promising anti-microbial activity when compared with the standards.


Author(s):  
Raghunath Pendru ◽  
Jagan Nadipelly ◽  
Jyothinath Kothapalli ◽  
Subbannayya Kotigadde

 Objective: It has been well documented that traditional medicinal plants confer considerable antimicrobial activity against various microorganisms.Methods: The present study was designed to evaluate the antimicrobial properties of aqueous and ethanolic extracts of the leaves of Syzygium caryophyllatum against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, and Vibrio cholerae. The aqueous extract was prepared using cold percolation method, and ethanolic extract was prepared in Soxhlet apparatus using ethyl alcohol. Antimicrobial activity is being determined by time-kill assay and minimum inhibitory concentration.Results: From our study, it is found that all tested organisms were sensitive to both aqueous and ethanolic extract of leaves of S. caryophyllatum. The ethanolic extract showed better antibacterial activity against S. aureus and S. typhi when compare to aqueous extract. The phytochemical analysis confirmed the presence of flavonoids, alkaloids, glycosides, steroids, phenols, tannins, and saponins in the ethanolic and aqueous extract of S. caryophyllatum. Conclusion: We hypothesize that these active phytoconstitutents might be responsible for the antimicrobial activity of this plant.


Author(s):  
Bikash Gupta ◽  
Biswajit Dash ◽  
Sujata Paul ◽  
Aditya Bora

Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.


Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.


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