scholarly journals Endophytic Fungi of Brunfelsia Uniflora: Isolation, Cryopreservation, and Determination of Enzymatic and Antioxidant Activity

2021 ◽  
Author(s):  
Sara Jane Marsola ◽  
Lais Freitas Jorge ◽  
Adriano Broges Menequeti ◽  
Miria Benetati Delgado Berteli ◽  
Thaís Emanuelle Feijó Lima ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Laccase Activity ◽  
Antioxidant Activities ◽  
Sucrose Solution ◽  
Biological Activities ◽  
Cellulase Activity ◽  
Total Phenolic ◽  
Malt Extract ◽  
Phenolic Contents

Abstract Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro , is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic , depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia ; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from B. uniflora and determine their cellulase, laccase, and antioxidant activities. Endophytic fungi were isolated from B. uniflora stems, cultured on wheat grains, immersed in a 150 g/L aqueous sucrose solution, and cryopreserved at −80°C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of DPPH• scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from B. uniflora stems and classified into 24 groups according to morphological similarities. B. uniflora was shown to harbor a great diversity of endophytic fungi . Mycelial viability was observed after 1 and 6 months of cryopreservation at −80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and DPPH• and FRAP activities.

scholarly journals Diversity of endophytic fungi from Vernonia amygdalina, their phenolic and flavonoid contents and bioactivities

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Praptiwi Praptiwi ◽  
Ahmad Fathoni ◽  
Muhammad Ilyas
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Total Phenolic ◽  
Vernonia Amygdalina ◽  
Phenolic Contents ◽  
Tlc Bioautography ◽  
Phenolic And Flavonoid

Abstract. Praptiwi, Fathoni A, Ilyas M. 2019. Diversity of endophytic fungi from Vernonia amygdalina, their phenolic and flavonoid contents and bioactivities. Biodiversitas 20: 436-441. There are nine endophytic fungi in total associated with stem and leaves of Vernonia amygdalina were determined for their total phenolic and flavonoids content as well as assessed for their antibacterial and antioxidant activities. Total phenolic and flavonoid contents were determined by spectrophotometric methods. The antibacterial activity was performed using Thin Layer Chromatography (TLC) - Bioautography assay against S.aureus and E.coli. The antioxidant activity was carried out using TLC- Bioautography by DPPH scavenging assay. Broth serial microdilution assay was used to determine the minimum inhibitory concentrations (MIC) and the half-maximum inhibitory concentration (IC50). Among the identified isolates, there was one isolate of Phomopsis sp., two isolates of Phoma sp., two isolates of Lasiodiplodia sp., two isolates of Colletotrichum sp. and two isolates of Phyllosticta sp. The results showed that the phenolic contents were ranging from 0.53-116.03 mg GAE/g extract, flavonoids contents were ranging from 81.12 to 390.21 mg quercetin equivalent (QE)/g extract. MICs of endophytic fungi against S.aureus and E.coli ranged from 256 to >256 µg/mL and >256 µg/mL respectively that categorized as moderate antibacterial activity. The antioxidant activity of endophytic fungi associated with V. amygdalina was >128 µg/mL, and the antioxidant activity index (AAI) was 0.24.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Efficacy of N-Methyl-N-Nitrosourea Mutation on Physicochemical Properties, Phytochemicals, and Momilactones A and B in Rice

2019 ◽  
Vol 11 (23) ◽  
pp. 6862 ◽  
Author(s):  
Kifayatullah Kakar ◽  
Tran Dang Xuan ◽  
Nguyen Van Quan ◽  
Imran Khan Wafa ◽  
Hoang-Dung Tran ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Functional Foods ◽  
Grain Quality ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Rice Grain ◽  
Rice Cultivars ◽  
Phenolic Contents ◽  
Phytochemical Compounds ◽  
Beneficial Traits

Attempts regarding the improvement and development of novel rice with better quality and higher productivity have been increasing. Among approaches, mutation is a direct alteration on the genome and considered as one of the most beneficial routes to acquire new beneficial traits in rice. An experiment was carried out to explore the effects of N-methyl-N-nitrosourea (MNU) mutation on the antioxidant activities, phytochemical compounds, and momilactones A (MA) and B (MB) in rice. Two rice cultivars, K1 (an original cultivar DT84) and K2 (mutated DT84), were examined. Antioxidant activities, phenolic compounds, and momilactones of the rice grain, husk, and straw portions were measured and quantified. Antioxidant activities were higher in grain and straw of K2, whereas K1 showed greater antioxidant activity in rice husk. Additionally, K2 displayed higher total phenolic contents (TPC) in grain and straw as well as lower of it in the husk, but these variations significantly differed only in the straw portion. An increase in total flavonoid contents (TFC) was observed in the husk of K1, while K2 significantly enhanced TFC in straw. Both MA and MB, two compounds obtaining antidiabetes, anticancer, antimicrobial, antigout, and antiobesity properties, were detected and quantified in grain, husk, and straw of K1 and K2 samples. Generally, the contents of MA were higher than MB in all tested portions of rice crop. MA and MB were higher in straw followed by those in husk and grain, respectively. K2 contained higher amounts of MA and MB in straw and husk, but lower contents in grain compared with those in K1. This study illustrates that MNU mutation can improve grain quality and enhance bioactive compounds in straw, husk, and grain of rice. This approach has the potential to develop functional foods from rice, and therefore help farmers in developing countries to improve value in rice production.

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

scholarly journals Phenolic Compounds and In Vitro Antibacterial and Antioxidant Activities of Three Tropic Fruits: Persimmon, Guava, and Sweetsop

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Li Fu ◽  
WenQing Lu ◽  
XiaoMin Zhou
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Multidrug Resistant ◽  
Total Phenolic ◽  
Food Preservatives ◽  
Phenolic Contents

In our previous study, we have found that persimmon, guava, and sweetsop owned considerably high antioxidant activity and contained high total phenolic contents as well. In order to further supply information on the antibacterial and antioxidant activity of these three tropic fruits, they were extracted by 80% methanol. We then examined the extractions about their phenolic compounds and also studied the extractions and phenolic contents about their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against twelve targeted pathogens including 8 standard strains (Staphylococcus aureus,Bacillus cereus,Staphylococcus epidermidis,Monilia albican,Escherichia coli,Salmonella typhimurium,Shigella flexneri, andPseudomonas aeruginosa) and 4 multidrug-resistant strains (methicillin-resistant Staphylococcus aureus, ESBLs-producingEscherichia coli, carbapenems-resistantPseudomonas aeruginosa, and multidrug-resistantAcinetobacter baumannii), which are common and comprehensive in clinic. We also employed two ways, that is, FRAP and TEAC, to evaluate their antioxidant activities, using ultraviolet and visible spectrophotometer. Our study indicated that the three tropical fruits possessed obvious antioxidant and antibacterial activity, which supported the possibility of developing the fruits into new natural resource food and functional food as well as new natural antimicrobial agent and food preservatives. Moreover, phenolic compounds detected in the fruits could be used as a potential natural antibacterial agent and antioxidant.

Antioxidant Activities and Phenolic Composition of Apple Peel, Core and Flesh Extracts on Selected Apple Cultivars

2012 ◽  
Vol 554-556 ◽  
pp. 1103-1109 ◽  
Author(s):  
Gui Zhi Zhang ◽  
Bao Ping Ji ◽  
Gang Chen
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Apple Cultivars ◽  
Apple Peels

Phenolic antioxidants have multiple benefits to human health. Polyphenols are responsible for the antioxidant activity in apples. Antioxidant activities were assessed using the ferric reducing/antioxidant power (FRAP) and 2,2-dipheny l-1-picrylhydrazyl (DPPH) radical scavenging assays in different cultivars and different parts of apples grown in China. The phenolic compounds and antioxidant activities differed significantly among the four apple cultivars. Guoguang had the highest phenolic concentration and antioxidant activity and Golden Delicious had the lowest. The peels had the highest total phenolic and flavonoid contents followed by the cores and flesh. Anthocyanins were detected only in red apple peels. Peel and core had greater antioxidant activities than apple flesh. FRAP values were inversely correlated with phenolic contents, whereas no clear relationship could be observed between DPPH values and phenolic contents. The higher levels of phenolic compounds and antioxidant activities of apple peels and cores than flesh may be of technological interest as a valuable source of antioxidants.

scholarly journals Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

2021 ◽  
Vol 4 (4) ◽  
pp. 301-310
Author(s):  
Irma Antasionasti ◽  
Olvie Syenni Datu ◽  
Utami Sasmita Lestari ◽  
Surya Sumantri Abdullah ◽  
Imam Jayanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Egg White ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Stable Free Radical ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Myristica Fragrans

The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals Comparison of Total Phenolic Contents and Antioxidant Activities of Centella asiatica Extracts Obtained by Three Extraction Techniques

2019 ◽  
Vol 6 (2) ◽  
pp. 42-49
Author(s):  
Wan Nurul Huda Wan Zainal ◽  
FARA RESTINA MUSAHIB ◽  
NABILAH SYAFIQAH ZULKEFLEE
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Phenolic Contents ◽  
Total Phenolic Contents

Through different extraction techniques, the potential of Centella asiatica as a natural source of antioxidant was investigated. The C. asiatica aqueous extracts were obtained via infusion, decoction and ultrasonic-assisted extraction (UAE) techniques. The effects of different extraction techniques were studied on the extraction yield, total phenolic contents and antioxidant activity. The total phenolic contents of the extracts and antioxidant activity were examined using the Folin-Ciocalteu’s reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. Results indicated that the UAE exhibited the highest extraction yield, highest total phenolic contents, as well as highest antioxidant activity. The yield of the extracts increased in the order of infusion < decoction < UAE which were 18.2 %, 23.6% and 25.4 %, respectively. All extraction techniques had a significant effect (p <0.05) on the total phenolic contents and antioxidant activity of C. asiatica extracts. The total phenolic contents was ranged from 3.42 ± 0.030 to 8.32 ± 0.105 mg GAE/g dry extract while the antioxidant activity was in the range of 75 to 86 %. This study confirms that C. asiatica has the potential to be a good resource for the future development of natural antioxidant. In addition, extraction via UAE can be an ideal technique to obtain phytochemical-rich extracts from medicinal plants.

scholarly journals ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENTS OF SEVEN BROWN SEAWEED FROM DJIBOUTI COAST

2019 ◽  
pp. 42-44
Author(s):  
ABDIRAHMAN ELMI FOURREH ◽  
FATOUMA MOHAMED ABDOUL-LATIF ◽  
MOUSTAPHA NOUR IBRAHIM ◽  
ALI MERITO ALI
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Dry Matter ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Brown Seaweed ◽  
Antioxidant Potential ◽  
Phenolic Contents ◽  
Dpph Method

Objective: This study focuses on the antioxidant potential of Djibouti brown seaweed and their phenolic contents. Methods: We evaluated the antioxidant potential by DPPH method (1,1-diphenyl-2-picrylhydrazyl) and their phenolic contents of seven Djibouti seaweed: Cytoseira myrica, Padina pavonica, Sargassum fluitans, Sargassum ilifolium, Sargassum sp, Turbinaria triquetra and Turbinaria turbinata. Also, we searched the secondary metabolites of these seaweeds. Results: We obtain a higher antioxidant activity at 60,7±0,9 % and a higher phenolic content at 199,01±0,5 μg equivalent phloroglucinol (PGE)/g dry matter for Padina pavonica. A good linear correlation (R2 = 0,898) is observed between the antioxidant activity and the phenolic content of the seaweed studied. Also, two Padina pavonica collected in two different locations have different biochemical concentrations and antioxidant activity, suggesting the influence of the marine environment on the biosynthesis of secondary metabolites and the biological activities of seaweed. The present study shows the presence of tannins, saponosides, flavonoids and steroid-terpenes. Conclusion: The species studied show interesting antioxidant activities and can be consumed to prevent oxidative stress.

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