STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS

2020 ◽  
pp. 70-74
Author(s):  
DEEPAK SHRESTHA ◽  
JITENDRA PANDEY ◽  
CHIRANJIBI GYAWALI ◽  
MAHESH LAMSAL ◽  
SUNITA SHARMA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Time Interval ◽  
Root Extract ◽  
Glucose Diffusion ◽  
Anti Oxidant

Objective: The systematic study of effective alternative anti-diabetic drugs has great importance to manage diabetes as well as other oxidative stress-related diseases. According to previous research, root and bark of Mussaenda macrophylla plant has anti-microbial, anti-coagulant, anti-inflammatory, and hepatoprotective activity. Ethnomedicinal data shows that Mussaenda macrophylla is used to treat diabetes as well as oxidative stress. The objective of this research is to investigate in vitro anti-diabetic and anti-oxidant activity of root extract of Mussaenda macrophylla. Methods: DPPH free radical scavenging assay was used to detect anti-oxidant potency of ethanol and methanol root extract of the plant and expressed as % of radicle inhibition. Anti-diabetic activity was determined by the glucose diffusion method using a glucose oxidase kit and results were expressed as mean±SD. Results: The ethanol root extract at the concentration of 50 mg/ml and 100 mg/ml showed better glucose diffusion inhibition than that of methanol extract at the same concentration on increasing time interval. Ethanol extract at the concentration 100 µg/ml displayed better DPPH scavenging activity (89.83±0.19 %) than that of methanol extract (86.61±0.75%). Conclusion: This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model.

Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata

2021 ◽  
pp. 2635-2638
Author(s):  
Mrinmoy Basak ◽  
Moksood Ahmed Laskar ◽  
Priyanka Goswami
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculata was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows 34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Salvia amplexicaulis Lam. extracts

2014 ◽  
Vol 66 (1) ◽  
pp. 307-316 ◽  
Author(s):  
Ana Alimpic ◽  
Mariana Oaldje ◽  
V. Matevski ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Whole Plant ◽  
Phenolic And Flavonoid

This study was designed to examine the in vitro antioxidant DPPH free radical-scavenging activity, and the total phenolic and flavonoid content of ethanol and methanol extracts of Salvia amplexicaulis Lam. in the whole plant and different parts, leaves, stems and flowers. The largest amounts of extract yield were obtained from the flowers, 14.14% and 12.00 % (w/w) in the ethanol of methanol extracts, respectively. The ethanol extract of leaves (16.07 ?g/ml) and methanol extract of the whole plant (21.28 ?g/ml) showed the highest activity against the DPPH radical. The ethanol extract of the leaves was the richest in phenols (222.40 mg GAE/g) and flavonoids (49.81 mg QE/g), whereas the methanol extract of the whole plant contained the highest amount of phenolics (180.89 mg GAE/g) and flavonoids (38.15 mg QE/g). A very strong linear correlation between antioxidant activity and the phenolic content of the extracts was established. The obtained results suggest that S. amplexicaulis could be regarded as an important source of natural antioxidants.

scholarly journals Etude phytochimique et activité antioxydante comparatives des trois variétés de Catharanthus roseus (L.) G. Don

2020 ◽  
Vol 14 (6) ◽  
pp. 2352-2361
Author(s):  
Hèzouwè Kagnou ◽  
Oudjaniyobi Simalou ◽  
Gneiny Whad Tchani ◽  
Sabrina Sanvee ◽  
Kokou Agbékonyi Agbodan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Catharanthus Roseus ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Hybrid Variety

Catharanthus roseus (L.) G. Don, est une plante médicinale largement utilisée en tradithérapie contre le diabète, l’hypertension, le cancer et les effets du stress oxydatif. Elle existe en trois variétés ("rosea", "alba" et "hybride") selon la couleur des fleurs. L’étude actuelle porte sur le criblage phytochimique et l’activité antioxydante in vitro (DPPH et FRAP) de l’extrait hydro-éthanolique des trois variétés. Les tests ont été effectués sur les extraits des feuilles, fleurs et racines. On a noté la présence des alcaloïdes, phénols, flavonoïdes, tanins, saponines, stérols, terpènes et quinines. Cependant, l’absence des sucres réducteurs, des flavonoïdes et des saponines dans les racines ainsi que les quinines dans les feuilles a été relevée. L'analyse comparative par piégeage du radical DPPH a montré que les extraits des fleurs et racines de l’hybride étaient plus actifs, respectivement de 130,023±3,2 et 131,189±1,8 mg EqQ/g. Une tendance différente a été observée dans les extraits des feuilles où la meilleure valeur DPPH de 65,746±2,5 mg EqQ/g est attribuée à "rosea" alors que celle du FRAP de 139,935±2,57 mg EqAA/g provient de "alba". Ces résultats devraient encourager la poursuite des travaux sur l'identification et la caractérisation de nouveaux composés bioactifs sélectivement abondant pour une meilleure valorisation de Catharanthus roseus sur le plan de phytomédicaments.Mots clés : Catharanthus roseus, variétés, criblage phytochimique, antioxydant. English Title: Phytochemical study and comparative antioxidant activity of the three varieties of Catharanthus roseus (L.) G. DonCatharanthus roseus (L.) G. Don, is a medicinal plant widely used in the treatment of diabetes, hypertension, cancer, and effects of oxidative stress. C. roseus exists in three varieties ("rosea", "alba" and "hybrid") depending on the colour of the flowers. This study focused on phytochemical screening and in vitro antioxidant activity (DPPH and FRAP) of the hydro-ethanol extract of the three varieties. The tests were carried out on leaves, flowers, and roots extracts. The presence of alkaloids, phenols, flavonoids, tannins, saponins, sterols, terpenes, and quinines were noted. However, the absence of reducing sugars, flavonoids, and saponins in the roots and quinines in the leaves was observed. Comparative analysis of DPPH radical scavenging showed that extracts from the flowers and roots of the hybrid were more active, respectively at 130.023±3.2 and 131.189±1.8 mg EqQ/g. FRAP values of the same samples were 175.166± 9.28 and 121.311±7.8 mg EqAA/g dry matter. For the leaves, the best DPPH value of 65.746±2.5 mg EqQ/g was attributed to "rosea" while the FRAP value of 139.935±2.57 mg EqAA/g was for "alba". These interesting antioxidant activities of the "hybrid" variety may be useful against oxidative stress pathologies. The current study might then promote the continuing identification and characterization of new bioactive compounds for a development of Catharanthus roseus.Keywords: Catharanthus roseus, varieties, phytochemical screening, antioxidant.  

ANALYSIS OF THE PHYTOCHEMICALS, ANTIOXIDANTS, AND ANTIMICROBIAL ACTIVITY OF FICUS TSJAHELA BURM. F LEAF, BARK, AND FRUIT EXTRACTS

2021 ◽  
pp. 54-59
Author(s):  
THAMARAIKANI V ◽  
AMALA S DIVYA ◽  
SEKAR T
Keyword(s):  
Methanol Extract ◽  
Experimental Studies ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Acetobacter Aceti ◽  
Saponin Content ◽  
Fruit Samples

Objective: Ficus tsjahela Burm. f is a medicinal tree species, endemic to the Western Ghats having various healing properties. This study focused to check the antioxidant and antimicrobial activities of the leaf, bark, and fruit samples of F. tsjahela. Methods: The plant samples were subjected to Soxhlet extraction for phytochemical analysis and further experimental studies. The test on phytochemical studies indicated the presence of alkaloids, saponins, glycosides, and flavonol glycosides within the plant parts, respectively. The estimation of alkaloids, saponins, in vitro antioxidant, and antibacterial activities revealed that the methanol bark extracts have high activity compared to others. Results: Total alkaloid and saponin content was found to be high in leaf methanol extract was 996.17 mg/g quinine equivalent/g and 957.3 mg/g diosgenin equivalent/g, respectively. In vitro antioxidant assays revealed a strong radical scavenging potential of the methanol bark extract against stable (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), phosphomolybdenum, and superoxide radicals. Agar well diffusion method has been used to determine the antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Acetobacter aceti, and Pseudomonas aeruginosa). Conclusion: The bark methanol extract of F. tsjahela has exhibited remarkable antioxidant activity and significant antibacterial activity (p<0.05) against all tested bacterial strains observed.

scholarly journals IN VITRO ANTIOXIDANT AND PHOTOPROTECTIVE ACTIVITIES OF CARICA PAPAYA FRUITS

2019 ◽  
pp. 308-310
Author(s):  
SEOW LAY JING ◽  
KHOR POH YEN ◽  
GOURI KUMAR DASH
Keyword(s):  
Ascorbic Acid ◽  
Aqueous Extract ◽  
Carica Papaya ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
World Health ◽  
Total Phenolic ◽  
Fruit Powder

Objective: The objective of the study was to evaluate the antioxidant and photoprotective effects of Carica papaya fruits using spectrophotometric methods. Methods: The dried fruit powder of C. papaya was extracted separately in methanol, ethanol, and distilled water for 2 days on a constant heating water bath at 60°C. The extracts were concentrated under vacuum using a rotary evaporator and separately dried in a desiccator to yield the methanol, ethanol, and aqueous extracts. Proximate analysis of the fruit powder such as determination of ash values, loss on drying, and extractive values was performed as per the World Health Organization guidelines. Total phenolic and flavonoid contents were determined by Folin–Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities of the extracts were evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging method and compared with ascorbic acid. The photoprotective effect was evaluated by the spectrophotometric method. Results: Our results revealed the total phenolic content 59.7±1.15 for methanol extract, 56.1±0.33 for ethanol extract, and 64.4±1.95 for the aqueous extract μg/ml of gallic acid equivalent. The level of flavonoids, expressed in quercetin equivalents in μg/ml, of methanol extract was of 79.3±0.61, ethanol extract 66.3±0.13, and aqueous extract 23.9±0.26, respectively. The free radical scavenging activity of the extracts was found superior to ascorbic acid that was used as the standard for activity comparison. The results of sun protection factor (SPF) in vitro study demonstrated comparable SPF in a concentration-dependent manner. Conclusion: The flavonoids and phenolic compounds present in the extracts could be responsible by the photoprotective effect of C. papaya.

scholarly journals The efficacy of hibiscus rosa-sinensis leaf extracts against candida SPP, causing candidiasis

2019 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
S. Zuhaira ◽  
Shaza Naz ◽  
P. M. Ridzuan
Keyword(s):  
Candida Species ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
World Health ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Candida Spp ◽  
Hibiscus Rosa Sinensis

Over the years, World Health Organization has recommended traditional medicines as safe remedies for ailments of both microbial and non-microbial origins. The use of herbals has increased worldwide in recent years, as they are believed to be safer than modern medicines with lesser or no side effects. The present study has been designed to determine the role of Hibiscus rosa-sinensis leaves extract in-vitro antifungal activities against Candida albicans, Candida tropicalis, and Candida glabrata species. The leaves of H. rosa-sinensis were soaked in methanol and ethanol for solvent extraction. The extraction yield was diluted in DMSO to obtain 6 different concentrations, and was then impregnated on blank discs. The disc diffusion method was used to test against 3 different candida species for sensitivity test. Basic phytochemical constituent’s detection on phenol, carbohydrate, tannins and saponins were done on H. rosa-sinensis. The extract that shows largest zone of inhibition of candida species is the leaf extract with ethanol. 1000mg/ml of the leaf extract shows the highest inhibition zone against C. albicans, C. tropicalis and C. glabrata which are 9mm, 8mm and 9mm respectively. Carbohydates test showed both extractions highly positive and ethanol extract exhibited the highest saponin compared with methanol extract. It was found that ethanol extract of H. rosa- sinensis showed more potent anti candidiasis compared with methanol extract.

scholarly journals Evaluation of Anti-Oxidant and Anti-Pyretic Activity of Fruit of Garcenia Padunculata

2020 ◽  
Vol 8 (3) ◽  
pp. 77-80
Author(s):  
Basak Mrinmoy ◽  
Laskar Ahmed Moksood ◽  
Goswami Priyanka
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculatawas collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.    

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

2021 ◽  
pp. 5217-5221
Author(s):  
Krishnaveni R ◽  
Rajan S.
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Rbc Membrane ◽  
Clerodendrum Phlomidis ◽  
Anti Oxidant ◽  
Anti Inflammatory

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

scholarly journals Kinetics of α-amylase and α-glucosidase Inhibitory Potential of Hermannia geniculata Eckl. & Zehl root Extracts Used in Basotho Traditional Medicine.

2020 ◽  
Vol 2 (2) ◽  
pp. 105-120
Author(s):  
L. A. Adeniran ◽  
A. O. T. Ashafa
Keyword(s):  
Diabetes Mellitus ◽  
Traditional Medicine ◽  
Competitive Inhibition ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Kinetic Studies ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Root Extract

The scientific investigation of the folkloric use of Hermannia geniculata roots in the management of diabetes mellitus was conducted. Phytochemical analyses, in vitro antioxidant and hyperglycaemic studies were carried out on the crude extracts of H. geniculata. Qualitative phytochemical analysis revealed the presence of saponins, phenols, flavonoids, alkaloids, tannins, phytosterols, triterpenes and anthraquinones. The ethanol extract exhibited the highest free radical scavenging capability with the lowest IC values (0.52, 0.38, 0.59, 0.63, 0.39) mg/mL for 50 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-Azino-bis(3-ethylbenzothiazoline-6-Sulphonic acid (ABTS), hydroxyl radical, superoxide anion radical and metal chelating ability which is significantly different (p<0.05) from the standard (silymarin). In antidiabetic studies, ethanol extract is a potent inhibitor of α-glucosidase (IC : 0.15 mg/mL) which is lower and significantly 50 different (p<0.05) from the standard (acarbose) IC value of (0.52 mg/mL). Ethanol extract 50 exhibited a milder inhibition of α-amylase enzyme with IC (0.57mg/mL) which is higher and 50 significantly different (p<0.05) from acarbose with IC (0.47 mg/mL). Kinetic studies revealed H. 50 geniculata ethanol extract exhibited competitive inhibition of α-amylase and uncompetitive inhibition of α-glucosidase enzymes. All these findings provided the scientific basis which support the use of the root extract of H. geniculata in the management of diabetes mellitus and oxidative stress induced ailments like colitis and ulcers by the Basotho traditional medicine of South Africa.

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