scholarly journals PRODUCTION AND PARTIAL CHARACTERIZATION OF PIGMENTS PRODUCED BY KOCURIA SP BRI 36: INFLUENCE OF HEAVY METALS

2017 ◽  
Vol 9 (10) ◽  
pp. 137 ◽  
Author(s):  
Anuradha Mulik ◽  
Priyanka Kumbhar ◽  
Rama Bhadekar
Keyword(s):  
Heavy Metals ◽  
Antimicrobial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pigment Production ◽  
Orange Pigment ◽  
Maximum Stability ◽  
Tlc Analysis ◽  
Physical And Chemical ◽  
Layer Chromatography

Objective: To study the production of pigments by Kocuria sp. BRI 36, their characteristics and influence of heavy metals on pigments.Methods: The effects of various physical and chemical parameters on pigments production by Kocuria sp. BRI 36 were examined. Pigments were extracted and partially characterised by Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR). The effects of heavy metals such as Pb2+, Cd 2+, Ni2+ and Cr3+ were studied on pigment production. Antimicrobial activity and stability studies of crude pigment were also conducted.Results: Kocuria sp. BRI 36 isolated from cold oceanic region maximally produced red-orange pigment in presence of glucose (5% w/v) and protease peptone (0.2% w/v) at pH 7.5, 10±1 °C. Thin layer chromatography (TLC) analysis revealed the occurrence of three different compounds in the crude pigment belonging to carotenoid and xanthophyll group. Metals like Ni2+ and Cr3+ adversely affected pigment production while Pb2+and Cd2+enhanced the yield. The significant features of Kocuria sp. BRI 36 pigment are i) antimicrobial activity against Gram-positive and Gram-negative bacteria, ii) maximum stability at pH 7.5 and 10±1 °C and iii) ~38% color loss at 50±1 °C in 5 h.Conclusion: Our results suggest application potential of Kocuria sp. BRI 36 pigments in various biotechnological fields.

scholarly journals TLC analysis of some phenolic compounds in kombucha beverage

2004 ◽  
pp. 199-205 ◽  
Author(s):  
Radomir Malbasa ◽  
Eva Loncar ◽  
Ljiljana Kolarov
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Green Tea ◽  
Black Tea ◽  
Wide Range ◽  
Tea Fungus ◽  
Natural Phenolic Compounds ◽  
Tlc Analysis ◽  
Layer Chromatography

Black and green tea contains a wide range of natural phenolic compounds Flavanoids and their glycosides, catechins and the products of their condensation, and phenolic acids are the most important. Kombucha beverage is obtained by fermentation of tea fungus on black or green tea sweetened with sucrose. The aim of this paper was to investigate the composition of some phenolic compounds, catechin, epicatechin, quercetin, myricetin, gallic and tanic acid, and monitoring of their status during tea fungus fermentation. The method used for this study was thin layer chromatography with two different systems. The main phenolic compounds in the samples with green tea were catechin and epicatechin, and in the samples with black tea it was quercetin.

Analysis of Dairy Products for Chlorinated Insecticide Residues by Thin Layer Chromatography

1966 ◽  
Vol 49 (4) ◽  
pp. 795-800
Author(s):  
William A Moats
Keyword(s):  
Aluminum Oxide ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Color Development ◽  
Tlc Plates ◽  
One Step ◽  
Rf Values ◽  
Tlc Analysis ◽  
Layer Chromatography

Abstract Butterfat and milk samples were analyzed for chlorinated insecticides by thin layer chromatography (TLC) on aluminum oxide or silica gel plates containing a small amount of silver nitrate. The adsorbent was washed with distilled water before preparing the plates. A one-step cleanup on a partially inactivated Florisil column was performed prior to TLC analysis. For color development, the TLC plates were sprayed lightly with hydrogen peroxide to suppress possible interference from fat and then steamed before exposure to ultraviolet light to accelerate and intensify the color reaction. Rf values for a number of solvent systems on aluminum oxide and silica gel plates are given. With this procedure, 0.05 μg or less of insecticide can be detected in a 0.4 g butterfat sample or the extract from 10 ml milk.

Toxicity tests in eight species of Chrysochromulina (Haptophyta)

1997 ◽  
Vol 75 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Susanne Simonsen ◽  
Øjvind Moestrup
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Artemia Salina ◽  
Toxicity Tests ◽  
Haemolytic Activity ◽  
Prymnesium Parvum ◽  
Unialgal Culture ◽  
Tlc Analysis ◽  
Layer Chromatography ◽  
Chloroform Methanol

Blooms of the marine flagellate Chrysochromulina have resulted in mortality of marine organisms in Scandinavian waters, including fish in aquaculture. Eight species of Chrysochromulina, namely C. apheles, C. brevifilum, C. ericina, C. hirta, C. leadbeateri, C. parva, C. polylepis, and C. simplex, isolated into unialgal culture, were examined for haemolytic activity and toxicity to the brine shrimp, Artemia salina. Haemolytic fractions were obtained from all species, but only C. polylepis cells were toxic to Artemia. Thin-layer chromatography (TLC) analysis in chloroform –methanol–water (75:25:4) and in chloroform–methanol (9:1) yielded up to six haemolytic spots. Except for one spot, these all occurred in extracts of the species examined, including Isochrysis sp., which was used as a control, C. polylepis, and the well-known fish killer Prymnesium parvum. The single unique haemolytic spot (Rf values 0.45 and 0.16 in solvents I and II, respectively) occurred in the extract from C. polylepis. When isolated by TLC, the contents of the single spot were toxic to Artemia. Key words: Chrysochromulina, toxicity, haemolytic, Artemia, thin-layer chromatography (TLC).

scholarly journals Synthesis and Antimicrobial Activity of (2-Methoxy/2-Amino)-6-{4'-[(4'''-Chlorophenyl) (Phenyl) Methyl Amino] Phenyl}-4-Aryl Nicotinonitrile

2015 ◽  
Vol 49 ◽  
pp. 99-103
Author(s):  
J.V. Guna ◽  
V.N. Bhadani ◽  
H.D. Purohit ◽  
Dipak M. Purohit
Keyword(s):  
Antimicrobial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spectral Data ◽  
Elemental Analysis ◽  
Mass Spectral Data ◽  
Mass Spectral ◽  
H Nmr ◽  
Bacteria And Fungi ◽  
Layer Chromatography

2- Methoxy – 6 - {4' - [(4'''- Chlorophenyl) (phenyl) methyl amino] phenyl} - 4 - aryl nicotinonitrile (3a-3l) and 2-Amino-6-{4'-[(4'''-Chlorophenyl)(phenyl)methyl amino]phenyl}-4-aryl nicotinonitrile (4a-4l) have been synthesized. The products have been assayed for their antimicrobial activity against Gram +ve, Gram -ve bacteria and fungi. The structure of the products has been elucidated by IR, 1H-NMR, mass spectral data, elemental analysis and thin layer chromatography.

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L).

2021 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Rizki Nisfi Ramdhini ◽  
Isna Mulyani ◽  
Syaikhul Aziz
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Waste Product ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Processing Industry ◽  
Arachis Hypogaea L ◽  
Tlc Analysis ◽  
Layer Chromatography

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

Bioguided identification of antimicrobial compounds from Chrysopogon zizaniodes (L.) Roberty root essential oil

2019 ◽  
Vol 14 (14) ◽  
pp. 1179-1189
Author(s):  
Román Yesid Ramírez-Rueda ◽  
Jane Marinho ◽  
Marcos José Salvador
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Relevant Compound ◽  
Vancomycin Resistant ◽  
Direct Bioautography ◽  
Layer Chromatography ◽  
Root Essential Oil

Aim: To determine the group of compounds from Chrysopogon zizaniodes root essential oil that have antimicrobial activity. Materials & methods: Thin-layer chromatography coupled to direct bioautography was used to determinate the fraction(s) having antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). Through GC-MS identification, the fractions with the greatest similarity to the active thin-layer chromatography fraction were used to determinate the MIC. Results: The subfraction 8 from column chromatography was responsible for the best MIC for MRSA (62.5 μg/ml) and VREF (125 μg/ml). Five compounds possibly responsible for antimicrobial activity were preliminary identified. Conclusion: We suggest that Cedr-8-en-13-ol, could be the more relevant compound involved in the antimicrobial activity in this study.

scholarly journals Separation of transition and heavy metals using stationary phase gradients and thin layer chromatography

2016 ◽  
Vol 1446 ◽  
pp. 141-148 ◽  
Author(s):  
Stacy L. Stegall ◽  
Kayesh M. Ashraf ◽  
Julie R. Moye ◽  
Daniel A. Higgins ◽  
Maryanne M. Collinson
Keyword(s):  
Heavy Metals ◽  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Transition And Heavy Metals ◽  
Layer Chromatography

Quality control, HPTLC analysis, antioxidant and antimicrobial activity of hydroalcoholic extract of roots of qust (Saussurea lappa, C.B Clarke)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Shabnam Ansari ◽  
Mohammad Maaz ◽  
Iftekhar Ahmad ◽  
Syed Kazim Hasan ◽  
Sajad Ahmad Bhat ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Quality Control ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Skin Diseases ◽  
Hydroalcoholic Extract ◽  
Saussurea Lappa ◽  
Antioxidant And Antimicrobial Activity ◽  
Layer Chromatography

AbstractObjectivesSaussurea lappa, CB Clarke (S.lappa) is a perennial herb of the Compositae family. The root of S.lappa has been used for the treatment of various diseases such as hepatitis, jaundice, intestinal worms, bronchial asthma, and a variety of skin diseases. The aim of the study was to ensure quality control of S.lappa and its preparation HAESL (hydroalcoholic extract of S.lappa) along with assessment of HAESL antimicrobial and antioxidant activities in vitro.MethodsHAESL was prepared with 50% ethanol, (v/v). Physiochemical analysis of the root of S.lappa, and phytochemical screening, thin-layer chromatography (TLC), high-performance thin-layer chromatography (HPTLC), and in vitro antimicrobial and antioxidant activity of HAESL were performed using the standard protocol.ResultsPhysiochemical and phytochemical assessments of S.lappa and HAESL showed the greater quality of the drug. HAESL showed the presence of many phytochemical constituents corresponding to colorful spots, peaks, area under the curve, and corresponding Rf values as evident in the TLC and HPTLC analysis. HAESL showed a concentration-dependent effect on radical scavenging activity against DPHH (2,2-diphenyl-1-picrylhydrazyl) and hydroxyl radical with IC50 value of 19.10 μg/mL and 82.23 μg/mL, respectively. HAESL exhibited antimicrobial activity against the growth of micro-organisms such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Aspergillus flavus, and Candida albicans. HASEL showed no antimicrobial activity against Aspergillus niger.ConclusionsThe procured and an identified sample of Saussurea lappa was good in quality and the prepared HAESL contained many phytochemical compounds. HAESL substantiated significant antioxidant and antimicrobial activity in vitro.

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