Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions

2021 ◽  
Vol 39 (4) ◽  
pp. 317-322
Author(s):  
Atef S. Abdel-Razek ◽  
◽  
Nesreen M. Abd El-Ghany ◽  
Mohamed A. Gesraha ◽  
Tarek A. Elewa ◽  
...  

Abdel-Razek, A.S., N.M. Abd El-Ghany, M.A. Gesraha, T.A. Elewa and A. Moussa. 2021. Susceptibility Assessment of Two Tomato Hybrids Against Tuta absoluta Infestation Under Greenhouse Conditions. Arab Journal of Plant Protection, 39(4): 317-322. https://doi.org/10.22268/AJPP-39.4.317322 Tuta absoluta is a major insect pest which attack tomato plant varieties in Egypt. Several control attempts were carried out to avoid major crop losses by heavy application of chemical insecticides. The aim of the present study is to assess the susceptibility of infestation of T. absoluta of two tomato varieties (Shifa and Savera F1 hybrids) under greenhouse conditions. The tomato varieties were planted in two plantation periods at the district of Kom Hamada, El-Nubaria province, El-Behira Governorate. The susceptibility tests were done by random counting of leaf samples for the presence of T. absoluta mines and larvae. Both tomato varieties showed almost the same T. absoluta infestation level. Moreover, yield assessment was carried out for the two plantation periods by taking the average fruit weight yield (Kg/acre). The tomato yield results showed that Savera F1 hybrid tomato had higher yield compared to Shifa F1 hybrid variety, but such difference was not significant, However, the yield difference of both vireties between the two planting dates was significant. Keywords: Tomato, Tuta absoluta, susceptibility, leaf-mine, larvae, yield.

Author(s):  
P. M. Akhmedova

In the conditions of covered crop in Dagestan tomato ranks the first in terms of cultivation area and in the country it is the second place after cucumber. However, tomato production has not yet reached the level necessary to meet people’s need. Growing it allows solving the problem of import substitution, ensures a constant supply of fresh vegetable products and solves the problem of a balanced, complete nutrition human nutrition throughout the year. When studying vegetable crops in covered crop conditions were revealed both general provisions characteristic of any region and specific features depending on the complex of local natural conditions. The level of radiation, temperature, humidity, wind and snow loads leave a certain influence on the growing tomatoes technology, which makes the problem of developing scientifically grounded elements of technologies for their production, ensuring high yields of vegetable plants and environmentally safe out-of-season products. With a wide variety of soil and climatic conditions in Dagestan, territories with extreme natural contrasts, even within a single agroclimatic region, the features of light and other microclimate regimes require clarification of the most important elements of agricultural technology. One of the main components of tomato growing technology is timely protection against diseases and pests. In the absence of a system of protective measures yield losses can reach 50% or more. In this context, the assessment of new F1 hybrids in film greenhouses in the winter-autumn circulation was carried out, the most promising of them for the conditions of Dagestan were identified, and the main elements of their cultivation were developed. The article presents the results of the dynamics of the yield of the crop by months. The influence of sowing and planting dates on the tomatoes yield is shown, the optimal scheme of sowing and planting tomatoes is determined. A phenological calendar for plant protection was compiled. The prospects of growing the studied hybrids in this region have been substantiated. Обоснована перспективность выращивания изучаемых гибридов в данном регионе.


Author(s):  
M. Nikornpun ◽  
K. Tunjai ◽  
K. Kaewsombat ◽  
T. Tarinta

Fruit yields and yield components of certain F1 hybrids, parental lines and commercial varieties in 3 discreet experiments were significantly different. Seven high-yielding F1 hybrids were observed: CA 1286 × CA 1447, CA1286 × CA1450, CA1303 × CA1446, CA1303 × CA1447, CA1303 × CA1450, CA1441 x CA1450 and CA1442 x CA1447. About half of them were significantly higher in yield than commercial varieties while the rest were not significantly different from the commercial varieties. Heterosis and heterobeltiosis of yield components of F1 hybrid chilis were significant. High yielding varieties showed both positive and significant heterosis and heterobeltiosis of all yield components. When maintainer lines, CA1441 and CA1442, were used as female parents, CA1441 was better than CA1442 for fruit length but variety CA1442 was better than variety CA 1441 for fruit width. When maintainer lines CA1286 and CA1303 were used as female parents, CA1303 was better than CA1286 in terms of yield in one experiment but they were more or less the samein another experiment. Significant general combining ability of the maintainer line CA1303 was observed in fruit width. Significant differences of general combining ability of some horticultural characteristics of restorers was observed in CA1446, CA1447, CA1448, CA1449 and CA1450. Almost no significant differences of specific combining ability of F1 hybrids were found in the F1 hybrids, except CA 1286 × CA 1447 and CA1303 × CA1451 demonstrated significant differences of specific combining ability of an average fruit weight.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Weitao Chen ◽  
Ming Zou ◽  
Yuefei Li ◽  
Shuli Zhu ◽  
Xinhui Li ◽  
...  

AbstractGenome complexity such as heterozygosity may heavily influence its de novo assembly. Sequencing somatic cells of the F1 hybrids harboring two sets of genetic materials from both of the paternal and maternal species may avoid alleles discrimination during assembly. However, the feasibility of this strategy needs further assessments. We sequenced and assembled the genome of an F1 hybrid between Silurus asotus and S. meridionalis using the SequelII platform and Hi-C scaffolding technologies. More than 300 Gb raw data were generated, and the final assembly obtained 2344 scaffolds composed of 3017 contigs. The N50 length of scaffolds and contigs was 28.55 Mb and 7.49 Mb, respectively. Based on the mapping results of short reads generated for the paternal and maternal species, each of the 29 chromosomes originating from S. asotus and S. meridionalis was recognized. We recovered nearly 94% and 96% of the total length of S. asotus and S. meridionalis. BUSCO assessments and mapping analyses suggested that both genomes had high completeness and accuracy. Further analyses demonstrated the high collinearity between S. asotus, S. meridionalis, and the related Pelteobagrus fulvidraco. Comparison of the two genomes with that assembled only using the short reads from non-hybrid parental species detected a small portion of sequences that may be incorrectly assigned to the different species. We supposed that at least part of these situations may have resulted from mitotic recombination. The strategy of sequencing the F1 hybrid genome can recover the vast majority of the parental genomes and may improve the assembly of complex genomes.


Author(s):  
Jin Yue Liu ◽  
Ze Wen Sheng ◽  
Yu Qi Hu ◽  
Qi Liu ◽  
Sheng Qiang ◽  
...  

AbstractThe releasing of transgenic soybeans (Glycine max (L.) Merr.) into farming systems raises concerns that transgenes might escape from the soybeans via pollen into their endemic wild relatives, the wild soybean (Glycine soja Sieb. et Zucc.). The fitness of F1 hybrids obtained from 10 wild soybean populations collected from China and transgenic glyphosate-resistant soybean was measured without weed competition, as well as one JLBC-1 F1 hybrid under weed competition. All crossed seeds emerged at a lower rate from 13.33–63.33%. Compared with those of their wild progenitors, most F1 hybrids were shorter, smaller, and with decreased aboveground dry biomass, pod number, and 100-seed weight. All F1 hybrids had lower pollen viability and filled seeds per plant. Finally, the composite fitness of nine F1 hybrids was significantly lower. One exceptional F1 hybrid was IMBT F1, in which the composite fitness was 1.28, which was similar to that of its wild progenitor due to the similarities in pod number, increased aboveground dry biomass, and 100-seed weight. Under weed competition, plant height, aboveground dry biomass, pod number per plant, filled seed number per plant, and 100-seed weight of JLBC-1 F1 were lower than those of the wild progenitor JLBC-1. JLBC-1 F1 hybrids produced 60 filled seeds per plant. Therefore, F1 hybrids could emerge and produce offspring. Thus, effective measures should be taken to prevent gene flow from transgenic soybean to wild soybean to avoid the production F1 hybrids when releasing transgenic soybean in fields in the future.


2017 ◽  
Vol 30 (11) ◽  
pp. 876-885 ◽  
Author(s):  
Jamuna Risal Paudel ◽  
Charlotte Davidson ◽  
Jun Song ◽  
Itkin Maxim ◽  
Asaph Aharoni ◽  
...  

Steroidal glycoalkaloids (SGAs) are major secondary metabolites constitutively produced in cultivated potato Solanum tuberosum, and α-solanine and α-chaconine are the most abundant SGAs. SGAs are toxic to humans at high levels but their role in plant protection against pests and pathogens is yet to be established. In this study, levels of SGAs in potato were reduced by RNA interference (RNAi)-mediated silencing of GLYCOALKALOID METABOLISM 4 (GAME4)—a gene encoding cytochrome P450, involved in an oxidation step in the conversion of cholesterol to SGA aglycones. Two GAME4 RNAi lines, T8 and T9, were used to investigate the effects of manipulation of the SGA biosynthetic pathway in potato. Growth and development of an insect pest, Colorado potato beetle (CPB), were affected in these lines. While no effect on CPB leaf consumption or weight gain was observed, early instar larval death and accelerated development of the insect was found while feeding on leaves of GAME4 RNAi lines. Modulation of SGA biosynthetic pathway in GAME4 RNAi plants was associated with a larger alteration to the metabolite profile, including increased levels of one or both the steroidal saponins or phytoecdysteroids, which could affect insect mortality as well as development time. Colonization by Verticillium dahliae on GAME4 RNAi plants was also tested. There were increased pathogen levels in the T8 GAME4 RNAi line but not in the T9. Metabolite differences between T8 and T9 were found and may have contributed to differences in V. dahliae infection. Drought responses created by osmotic stress were not affected by modulation of SGA biosynthetic pathway in potato.


Author(s):  
Hazel Cooley ◽  
Mario Vallejo-Marín

Abstract Buzz-pollinated plants require visitation from vibration producing bee species to elicit full pollen release. Several important food crops are buzz-pollinated including tomato, eggplant, kiwi, and blueberry. Although more than half of all bee species can buzz pollinate, the most commonly deployed supplemental pollinator, Apis mellifera L. (Hymenoptera: Apidae; honey bees), cannot produce vibrations to remove pollen. Here, we provide a list of buzz-pollinated food crops and discuss the extent to which they rely on pollination by vibration-producing bees. We then use the most commonly cultivated of these crops, the tomato, Solanum lycopersicum L. (Solanales: Solanaceae), as a case study to investigate the effect of different pollination treatments on aspects of fruit quality. Following a systematic review of the literature, we statistically analyzed 71 experiments from 24 studies across different geopolitical regions and conducted a meta-analysis on a subset of 21 of these experiments. Our results show that both supplemental pollination by buzz-pollinating bees and open pollination by assemblages of bees, which include buzz pollinators, significantly increase tomato fruit weight compared to a no-pollination control. In contrast, auxin treatment, artificial mechanical vibrations, or supplemental pollination by non-buzz-pollinating bees (including Apis spp.), do not significantly increase fruit weight. Finally, we compare strategies for providing bee pollination in tomato cultivation around the globe and highlight how using buzz-pollinating bees might improve tomato yield, particularly in some geographic regions. We conclude that employing native, wild buzz pollinators can deliver important economic benefits with reduced environmental risks and increased advantages for both developed and emerging economies.


2018 ◽  
Author(s):  
Daniel Oreperk ◽  
Sarah A Schoenrock ◽  
Rachel McMullan ◽  
Robin Ervin ◽  
Joseph Farrington ◽  
...  

ABSTRACTParent-of-origin effects (POEs) in mammals typically arise from maternal effects or from imprinting. Mutations in imprinted genes have been associated with psychiatric disorders, as well as with changes in a handful of animal behaviors. Nonetheless, POEs on complex traits such as behavior remain largely uncharacterized. Furthermore, although perinatal environmental exposures, such as nutrient deficiency, are known to modify both behavior and epigenetic effects generally, the architecture of environment-by-POE is almost completely unexplored. To study POE and environment-by-POE, we employ a relatively neglected but maximally powerful POE-detection system: a reciprocal F1 hybrid population. We exposed female NOD/ShiLtJxC57Bl/6J and C57Bl/6JxNOD/ShiLtJ mice, in utero, to one of four different diets, then after weaning recorded their whole-brain gene expression, as well as a set of behaviors that model psychiatric disease. Microarray expression data revealed an imprinting-enriched set of over a dozen genes subject to POE; the POE on the most significantly affected gene, Carmil1 (a.k.a. Lrrc16a), was validated using qPCR in the same and in a new set of mice. Several behaviors, especially locomotor behaviors, also showed POE. Interestingly, Bayesian mediation analysis suggests Carmil1 expression suppresses behavioral POE, and Airn suppresses POE on Carmil1 expression. A significant diet-by-POE was observed on one behavior, one imprinted gene, and over a dozen non-imprinted genes. Beyond our particular results, our study demonstrates a reciprocal F1 hybrid framework for studying POE and environment-by-POE on behavior.


2021 ◽  
pp. 58-64
Author(s):  
I. S. Mastyaev ◽  
A. F. Agafonov ◽  
L. V. Krivenkov

Relevance. The success of breeding work is largely determined by the source material, the search and creation of which for target breeding begins with the collection and study of collectible samples for the alignment of morphological features; for the precocity and amity of maturation; for the keeping quality and transportability of bulbs; for the quality of vegetable products (high content of biologically active substances and antioxidants); for resistance to biotic and abiotic stressors of a specific growing zone.Materials and methods. On the basis of the North Caucasus branch FSBSI FSVC, 90 samples of spring onions from 26 countries of the world were evaluated in the number nursery for the period from 2017 to 2019, 71 of them were varieties and 19 were F1 hybrids. In a hybrid nursery, 25 hybrid combinations obtained from FSBSI FSVC (VNIISSOK) were evaluated. The laying and placement of experiments, agricultural techniques of cultivation, accounting and evaluation of economically valuable traits, biochemical analyses were carried out according to generally accepted methods. The standards are the new Primo and Ampex varieties of the selection of the FSBSI FSVC and the Italian F1 hybrid Ranko, which were placed every 10 collection samples.Results.According to the results of the assessment, among the studied F1 varieties and hybrids, sources of economically valuable traits such as yield (20-27 t/ha), precocity (80-90 days), preservation (more than 85%) when laid for long-term storage up to 7 months, purple, white and pink color of bulbs, as well as sources of long-shaped bulbs were identified. The greatest interest for breeding for yield is represented by 9 promising varieties and 9 F1 hybrids of onions of various origins, as well as 4 hybrid combinations that were distinguished by high yields, the yield of marketable products and the mass of marketable bulbs. These samples are recommended for use in the breeding process as sources for the creation of new varieties and hybrids with high yields for the agro-climatic conditions of the foothill zone of the North Caucasus.r breeding


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
S. Kalaiselvan ◽  
S. Anuja

The present investigation consisted of 9 lines, 3 testers with their 27 F1 hybrids developed through line x tester mating fashion in randomized block design with three replications. Parents and hybrids differed significantly for gca and sca effects, respectively. Among the parents, maximum gca effect was found in EC 112241 and Aruna for inter node distance, EC 112241 and Arka Anamika for single fruit weight, EC 109454, EC 169344 and Arka Anamika for number of branches per plant, EC 169331 and Arka Anamika for number of fruits per plant, EC 109454 and Aruna for plant height at final harvest. The crosses exhibited maximum positive significant sca effect was observed in EC 112241 x Aruna, EC 109454 x Kashi Kranti and EC 112264 x Aruna were found as good specific combiners for single fruit weight, number of branches per plant and number of fruits per plant respectively. The hybrid EC 112264 x Aruna exhibited maximum significant negative sca effects for inter node distance and plant height at final harvest.


2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Zulfikar D Sahid ◽  
MUHAMAD SYUKUR ◽  
AWANG MAHARIJAYA

Abstract. Sahid ZD, Syukur M, Maharijaya A. 2020. Genetic diversity of capsaicin content, quantitative, and yield component in chili (Capsicum annuum) and their F1 hybrid. Biodiversitas 21: 2251-2257. Chili (Capsicum annuum L.) is one of the horticultural plants that have many benefits. The benefit of chili was determined by pungency level of its fruit. Pungency level of the chili is due to the capsaicin content in fruit. Information about the genetic diversity of capsaicin is still rarely available. The aims of this study were to obtain diversity information on quantitative, yield component, and capsaicin content, and to analyze the correlation among chili genotypes based on their morphological characters. This study used Randomized Complete Block Design with three replications. The genetic material used in this study consisted of 21 genotypes consisting of 6 genotypes of chili elders and 15 hybrid F1 genotypes resulting from their crossing. Six genotypes of the chili parents are C5, F6074, F9160291, Yuni, Bara, and Giant. 15 hybrid F1 genotypes used in this study are C5 x Bara, C5 x F6074, C5 x Yuni, C5 x Giant, C5 x F9160291, Bara x F6074, Bara x Yuni, Bara x Giant, Bara x F9160291, F6074 x Yuni, F6074 x Giant, F6074 x F9160291, Yuni x Giant, Yuni x F9160291, and Giant x F9160291. The observation was made on the variables of quantitative, yield, and capsaicin components on chili. The results showed that the highest capsaicin content only was found in Bara x F9160291. The results of scatterplot analysis showed that the highest capsaicin and yield component was found in BaraxF6074 and C5 x Yuni genotype. The results of cluster analysis showed that chili was clustered into three color groups. The character of capsaicin content is negatively correlated and very different from fruit weight, fruit diameter, fruit length, thick fruit flesh, total amount of fruit per plant, and fruit weight per plant.


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