scholarly journals Phytochemical screening, antioxidant and antimicrobial activities of Prunella vulgaris for oral thrush

2018 ◽  
Vol 8 (5-s) ◽  
pp. 251-258
Author(s):  
Amit Nayak ◽  
Mohammed Azaz Khan ◽  
Poornima Sharma ◽  
RM Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Quantitative Analysis ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Aluminium Chloride ◽  
Diffusion Method ◽  
Prunella Vulgaris

Plant imitative products have been used for medicinal purposes for centuries. In traditional Indian medicine or Ayurveda, Prunella Vulgaris and many other herbs have been used as medicine. Traditional uses of plants have led to investigating their bioactive compounds, which have resulted in the detection of a significant number of therapeutic properties. The aim of present investigation was carried out to evaluate the phytochemical, antioxidant and antimicrobial activity of chloroform and hydroalcoholic leaves extracts of Prunella Vulgaris against microbial strains causing oral infections. Both chloroform and hydroalcoholic extracts revealed the presence of carbohydrate, triterpenoids/ steroids, flavonoids, tannin, phenolic compound and saponins were absent in only the chloroform extract. The bioactivities of the leaf extracts were qualified to their phytochemical constituents. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of chloroform and hydroalcoholic leaves extracts of Prunella Vulgaris was assessed against 2,2-diphenyl-1-picryl- hydrazyl (DPPH) radical scavenging activity, reducing power assay using standard protocols. The antimicrobial activity of chloroform and hydroalcoholic extracts of medicinal plants was evaluated using well diffusion method against Escherichia coli and Candida albicans. The TPC in chloroform extract was higher than that of the hydroalcoholic extract with concentration being 0.443 mg/g equivalent to gallic acid. The TFC in hydroalcohoilc extract was higher than that of the chloroform extract with concentration being 0.358 mg/g equivalent to rutin.  The present study recognized leaves extract of Prunella vulgaris as a promising antioxidant and antimicrobial agent. However, further investigations are needed to understand the mechanistic basis of this effect of the extract and its chemical constituents thereof. Keywords: Prunella vulgaris, Phytochemical, Antioxidant, Antimicrobial activity, Folins Ciocalteau reagent, Quantitative analysis

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

scholarly journals Phytochemical evaluation and in vitro antioxidant studies of selected species of Gomphostemma Wall. ex Benth. from Western Ghats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 32-37
Author(s):  
M. K Sajitha ◽  
J. E. Thoppil
Keyword(s):  
Hydroxyl Radical ◽  
Western Ghats ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Dpph Assay ◽  
Radical Scavenging Assay

Plants are a rich source of bioactive compounds and have played a vital role in traditional medicine. Plant derived compounds are proved to have less side effects as compared to synthetic compounds. However, a truncated history on the contribution of plants to medicine together with lack of validation of plant derived components have led to massive research in phytochemistry and allied fields. Present study was intended to identify as well as to assess and compare different phytochemicals and antioxidant potential of selected taxa of Gomphostemma endemic to Western Ghats. Methanolic extracts of aerial parts of Gomphostemma heyneanum var. heyneanum, Gomphostemma heyneanum var. rottleri and Gomphostemma eriocarpum were subjected to preliminary phytochemical screening. Quantitative estimation of phenols and flavonoids was done with Folin-Ciocalteu method and modified Aluminium chloride method respectively. Antioxidant potential was examined by DPPH assay, Hydroxyl radical scavenging assay and Reducing power activity. Preliminary screening indicated the presence of many secondary metabolites like phenols, flavonoids, alkaloids, terpenoids and steroids. Results of quantitative estimation revealed that G. heyneanum var. heyneanum has the highest concentration of phenols and flavonoids. Antioxidant potential determined in terms of inhibition percentage also displayed that Gomphostemma species have significant radical scavenging activity. The antioxidant potential exhibited by the plant species may be attributed to their phenol and flavonoid content. Keywords: Phytochemical constituents, Gomphostemma, Quantitative estimation, Total phenolics, Flavonoids, Antioxidant activity, DPPH assay, Hydroxyl radical scavenging assay, Reducing power activity.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTI-CANCER AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS AND FRACTIONS OF STEPHANIA GLABRA

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Prachi Vaid ◽  
Adesh K. Saini ◽  
Reena V. Saini ◽  
Reena V. Saini ◽  
Reena V. Saini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Anti Cancer ◽  
Powder Analysis

Stephania glabra is a wild medicinal plant possessing multiple uses as anti-cancer, antimicrobial and antioxidant activities. Different extracts and fractions were prepared from S. glabra tubers and were analyzed for different activities. Evaluation of the total phenolic and flavonoid content revealed that chloroform extract of S. glabra contain maximum phenolic content and maximum flavonoid content was found in methanolic extract. Dry powder analysis of the tubers revealed the presence of saponins, alkaloids and proteins. Ethyl acetate extract of S. glabra tubers showed highest antioxidant potential as revealed by ABTS radical scavenging activity. Methanolic extract has the highest anti-cancer activity while chloroform extracts and fractions have highest antimicrobial activity. These activities can be attributed to the high amount of the phytoconstituents present in these extracts

scholarly journals Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus

2016 ◽  
Vol 4 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Nita Thapa ◽  
Pratiksha Thapa ◽  
Jay Bhandari ◽  
Prasodhan Niraula ◽  
Nikita Shrestha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Diffusion Method ◽  
Negative Consequences ◽  
Aqueous Methanol ◽  
Artocarpus Heterophyllus

In today’s world, search for natural medicines is increasing as a result of drug resistance of pathogens and also due to negative consequences of antibiotic. Presence of phytochemicals, antioxidant potential and antimicrobial activity of Artocarpus heterophyllus was carried out in this study. Leaf of this plant was subjected to warm extraction with three different solvents namely methanol, aqueous methanol and ethyl acetate. Leaf extract showed the presence of coumarin, alkaloid, terpenoid in methanol solvent; tannin, coumarin, saponin in aqueous methanol extract and coumarin, terpenoids in ethyl acetate solvent. Further, antimicrobial activity was assessed through disc diffusion method with six pathological bacteria and two fungi strains in four different concentrations of plant extract. Largest ZOI of 16mm was obtained against B. subtilis in 200mg/ml concentration for ethyl acetate extract. Antioxidant potential was measured by DPPH (Diphenyl-2-picrylhydrazyl) assay. DPPH free radical Scavenging Activity was expressed in % inhibition with L Ascorbic acid as standard and leaf extract in methanol showed the best activity.

Antioxidant and antimicrobial activity of lecithin free egg yolk protein preparation hydrolysates obtained with digestive enzymes

2012 ◽  
Vol 2 (12) ◽  
pp. 487 ◽  
Author(s):  
Aleksandra Zambrowicz ◽  
Marta Pokora ◽  
Ewelina Eckert ◽  
Marek Szołtysik ◽  
Anna Dąbrowska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Egg Yolk ◽  
Yolk Protein ◽  
Bacillus Species

Several biological activities have now been associated with egg protein- derived peptides, including antihypertensive, antimicrobial, immunomodulatory, anticancer and antioxidant activities, highlighting the importance of these biopeptides in human health, and disease prevention and treatment. Special attention has been given to peptides with antioxidant and antimicrobial activities as a new source of natural preservatives in food industry. In this study, the antioxidant properties of the egg-yolk protein by-product (YP) hydrolysates were evaluated based on their radical scavenging capacity (DPPH), Fe2+chelating effect and ferric reducing power (FRAP). Furthermore, antimicrobial properties of obtained hydrolysates against Bacillus species were studied. The degrees (DHs) of hydrolysis for 4h hydrolysates were: 19.1%, 13.5% and 13.0%, for pepsin, chymotrypsin and trypsin, respectively. Pepsin was the most effective in producing the free amino groups (1410.3 μmolGly/g). The RP-HPLC profiles of the protein hydrolysates showed differences in the hydrophobicity of the generated peptides. Trypsin hydrolysate obtained after 4h reaction demonstrated the strongest DPPH free radical scavenging activity (0.85 µmol Troloxeq/mg). Trypsin and chymotrypsin hydrolysates obtained after 4h reaction exhibited 4 times higher ferric reducing capacity than those treated by pepsin. The hydrolysis products obtained from YP exhibited significant chelating activity. The 4h trypsin hydrolysate exhibited weak antimicrobial activity against B. subtilis B3; B. cereus B512; B. cereus B 3p and B. laterosporum B6. Keywords: Antioxidative activity, DPPH, peptides, hydrolysis, egg-yolk protei

Antioxidant Activity of Lichen Parmotrema austrosinense and its Antimicrobial Effect on Selected Plant Pathogens

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Shyam Kumar Rajaram ◽  
Ganesh Moorthy Innasimuthu ◽  
Ronaldo Anuf Alexander ◽  
Rakesh Varghese ◽  
Murugan Munusamy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Benzene Extract

Parmotrema austrosinense(Zahlbr.) Hale is one of the lichens used as spices and also it has medicinal property. The goal of this investigation was to assess the antioxidant, reducing power, free radical scavenging and antiphyto pathogenic effect of P. austrosinense. Lichen sample was extracted with different solvents - benzene, methanol, chloroform and acetone. The benzene extract showed a strong antioxidant activity (5.665±0.0065) followed by chloroform extract (3.648±0.0029), acetone extract (2.770±0.0078) and methanol extract (2.430± 0.0046). The methanolic extract of lichen showed the highest reducing power followed by the acetone and nonpolar solvents like chloroform and benzene. Among the four solvents chosen, methanol extract of lichen showed the highest free radical scavenging activity followed by acetone, chloroform and benzene. The antimicrobial activity non-polar solvents like chloroform and benzene extract showed highest antimicrobial activity against three tested fungi (Fusarium oxysporum, Rhizoctonia solani, Sclerotium rolfsii) and bacterial plant pathogens(Bacillus sp.,Xanthomonas phaseoli and Erwinia chrysanthemi)followed by methanol and acetone extracts and streptomycin was used as a control antibiotic.

scholarly journals SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF INDOLE DERIVATIVES BEARING BENZIMIDAZOLE/BENZOTHIAZOLE MOIETY

2017 ◽  
Vol 9 (8) ◽  
pp. 128 ◽  
Author(s):  
Basavaraj Shivabasappa Naraboli ◽  
Jayaprakash Sharanappa Biradar
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biological Evaluation ◽  
Diffusion Method ◽  
Scavenging Activity ◽  
Indole Derivatives ◽  
Indole Ring ◽  
Ferric Ions ◽  
Antioxidant Power

Objective: The present work deals with the synthesis and characterization of biologically active new indole derivatives viz., 2-((1H-benzo[d]imidazol-2-yl)thio)-N-(4-(3-(1H-indol-3-yl)acryloyl)phenyl)acetamide 6a-d, N-(4-(3-(1H-indol-3-yl)acryloyl)phenyl)-2-((5-methoxy-1H-benzo[d]imidazol-2-yl)thio)acetamide 7a-d and N-(4-(3-(1H-indol-3-yl)acryloyl)phenyl)-2-(benzo[d]thiazol-2-ylthio)acetamide 8a-d.Methods: All these newly synthesized compounds were screened for their in vitro antimicrobial activity by an agar plate diffusion method, antioxidant activities: like 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity (RSA), ferric ions (Fe3+) reducing antioxidant power (FRAP), ferrous (Fe2+) metal ion chelating activity.Results: The structures of all the newly synthesized compounds were characterized by their IR, 1HNMR, mass spectral studies and elemental analysis. Compounds 6b, 7a, 7b, 8a and 8b exhibited good radical scavenging activity (RSA) at a concentration 100 µg/ml, compounds 7b, 7c, 7d and 8b displayed good ferric ions (Fe3+) reducing antioxidant power (FRAP) at a concentration 100 µg/ml, compounds 7b, 7d, 8b and 8d showed good ferrous (Fe2+) ion metal chelating activity. Compounds 7d and 8d exhibited good activity against all the screened bacteria and fungi.Conclusion: The synthesised compounds 7d having methoxy group at 5 positions of benzimidazoles ring, bromo group at 5 positions of indole ring, and 8d having bromo group at position 5 of indole ring, both the compounds have exhibited potent antimicrobial activity. Some compounds have shown very good antioxidant activity.

scholarly journals Antioxidant and Antimicrobial Activities of Withania frutescens

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601
Author(s):  
Laila El Bouzidi ◽  
Mustapha Larhsini ◽  
Mohamed Markouk ◽  
Abdelaziz Abbad ◽  
Lahcen Hassani ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Anticandidal Activity ◽  
First Time ◽  
Antioxidant And Antimicrobial Activities

In the present study, we report for the first time the antioxidant and antimicrobial activities of Withania frutescens (L.) Pauquy roots and leaves. Total phenolic content was determined using the Folin-Ciocalteu method and antioxidant activity was evaluated by the DPPH free radical scavenging and reducing power methods. Antimicrobial activity tests were carried out against ten bacterial species involved in nosocomial infections and two opportunistic clinical yeast isolates. The ethyl acetate and n-butanol leaf fractions exhibited the highest DPPH radical scavenging activity with IC50 = 4.53 ± 0.12 and 8.49 ± 0.46 μg/mL, respectively. The n-butanol root fraction showed the greatest reducing power comparable with that of quercetin at 0.4 mg/mL. The dichloromethane leaf fraction exhibited the highest antibacterial activity against both Gram-positive and Gram-negative bacteria with MIC values ranging between 50 and 400 μg/mL, depending on the tested bacteria. However, none of the examined extracts exhibited anticandidal activity. The polyphenol and glycowithanolide constituents appeared to be responsible for the antioxidant capacity of W. frutescens, whereas the observed antimicrobial activity may be due to the presence of withanolides.

scholarly journals Antioxidant, Antimicrobial Activity and Toxicity Test ofPilea microphylla

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Amir Modarresi Chahardehi ◽  
Darah Ibrahim ◽  
Shaida Fariza Sulaiman
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Toxicity Test ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPilea microphylla(Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at72.10±0.71and chloroform extract of method II (CE II) showed the highest total flavonoid contents at60.14±0.33. The antimicrobial activity ofPilea microphyllaextract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). ThePilea microphyllaextract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.

scholarly journals In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

2019 ◽  
Vol 19 (3) ◽  
pp. 100-108
Author(s):  
K. Thanzami ◽  
B.B. Kakoti ◽  
C. Lalremruati
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
In Vitro Models ◽  
Percent Inhibition ◽  
Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

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