scholarly journals Effect Of Suvarna Bhasma (Gold Calx) On Reproductive System Of Male Albino Rats

2021 ◽  
Vol 11 (6) ◽  
pp. 61-65
Author(s):  
Godatwar P ◽  
Prasad Bs ◽  
Skandhan Kp ◽  
Mehra Bl ◽  
Singh G ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Histological Study ◽  
Physiological Activity ◽  
Treated Group ◽  
Pure Metal ◽  
Albino Rats ◽  
Human Seminal Plasma ◽  
Fixed Amount ◽  
Testicular Weight

Suvarna Bhasma” (gold calx) (SB) prepared as per Ayurveda, a practicing medical system, is prescribed as a medicine in several ailments including conditions like oligozoospermia and asthenoszoospermia in male infertility. SB is prepared from pure metal gold by one of the processing methods detailed in Ayurveda classics. Gold along with other elements was reported in normal human seminal plasma. . In human pathological semen samples, the level of gold was less than normal. Gold may have a role in physiological activity of sperm as shown in case of other elements. The present study supports the hypothesis that the presence of gold is essential for sperm motility. Here we studied the effect of SB on male reproductive organs and semen from epididymis of rat. Present study was performed using Charles foster strain albino rats. Rats were divided into two groups of ten each namely, Group 1 (control) and Group 2 (study). Animals in study group were orally given a fixed amount of SB for 15 days. At the end of the study treated animals showed increase in body weight (<0.05) and testicular weight. In treated animals total sperm count (<0.05) and percentage of sperm motility were increased in epididymal fluid. Histological study showed increase in interstitial area of testes (<0.001), proliferation and branching of the epithelial layer of seminal vesicle. In the Study (gold treated) Group, the increased gold level in genital system may be responsible for the increase in sperm production and motility.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

Biosafety assessment of Fig (Ficus carica) leaves, an alternative preservative agent

2021 ◽  
Author(s):  
Olufemi Emmanuel Bankefa ◽  
Ruth Adefolakemi Gabriel-Ajobiewe ◽  
Olubunmi Evelyn Ajayi ◽  
Simbiat Olufunke Ayilara-Akande ◽  
Seye Julius Oladeji ◽  
...  
Keyword(s):  
Histological Study ◽  
Basal Medium ◽  
Basal Diet ◽  
Treated Group ◽  
Blood Parameters ◽  
Ficus Carica ◽  
Albino Rats ◽  
Limited Information ◽  
Chemical Toxicity

Due to the global disturbance of chemical resurgence in food, Fig leaves (Ficus carica) had been made to be applied as preservatives to extend the shelf life of perishable foods and to address chemical toxicity concern, despite limited information regarding its biosafety. Hence, the need to affirm the safety of this plant in-vivo was studied. Extracts of Fig leaves were mixed with basal diet and used as feed for one group of Wistar albino rats (treated group) while the second group was fed with sole basal medium to serve as control. The blood samples of both groups were taken before the rats were subjected to cervical dislocation. Haematological and histological parameters were studied to know the effects of the extracts and in order to justify the usage of plant extracts as preservatives for perishable foods. The haematological parameters of the treated group were higher than the control except for the insignificant changes in lymphocytes, basophils and eosinophils. Furthermore, the histological study revealed no sign of lesions in the all organs examined from both groups. The adoption of Fig leaf as preservatives has no contra-indication on the organs or blood parameters. Hence the global adoption as alternative preservative agents is undoubtedly recommended.

scholarly journals Effect of vitamin-E on cypermethrin induced toxicity in cerebral cortex of wistar albino rats: A histological study

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Samyog Mahat ◽  
Chandra Bhusan Jha ◽  
Shamsher Shrestha ◽  
Sarun Koirala
Keyword(s):  
Vitamin E ◽  
Glial Cell ◽  
Histological Study ◽  
Harmful Effect ◽  
Treated Group ◽  
Brain Weight ◽  
Albino Rats ◽  
Agricultural Field ◽  
Pyrethroid Pesticides ◽  
Wistar Albino Rats

Backgrounds: Cypermethrin, class II pyrethroid pesticides, is commonly used in the agricultural field and house pest controls. Cypermethrin leads to neurotoxicity by crossing the blood-brain barrier, inducing oxygen stress in brain tissue. Vitamin-E is an antioxidant, involving in protecting membrane stability against free radicals induced peroxidants. This study was conducted to see the protective effect of vitamin-E on cypermethrin induced toxicity. Methodology: Fifty healthy Wistar albino rats of either sex weighing 150-200 grams were randomly selected & divided into five groups. Rats in first group served as control. Rats in second and fourth group were given cypermethrin orally at 20 mg/kg/BW for 14 and 28 days respectively. Rats in third and fifth group were give cypermethrin plus vitamin-E 2.5 ml/kg/BW orally for 14 and 28 days respectively. Rats were sacrificed and the brain was removed. Tissue processing was done. Histological analysis was carried out and statistical analysis was performed by the use of SPSS. Result: Cypermethrin treated rats showed decreased body and brain weight with severe neuronal vacuolation and decrease glial cell. While cypermethrin plus vitamin-E treated group showed increased body and brain weight, with normal glial cell and mild neuronal changes were observed. Conclusion: Cypermethrin in combination with vitamin-E minimized its harmful effect which was evidenced by improving body and brain weight gain and histological changes.

scholarly journals Toxicological evaluation of a Nigeria-made polyherbal product on selected reproductive functions in adult male Wistar rats

2021 ◽  
Vol 11 (3) ◽  
pp. 001-008
Author(s):  
Oluwaseyi Adegoke Adetunji ◽  
Oluwafunmilola Christiana Adetunji ◽  
Grace Aimalohi Agbebaku-Izobo ◽  
Gogonte Hezekiah Amah ◽  
Opeyemi Adebola Adetunji ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Sperm Motility ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Count ◽  
Herbal Product ◽  
Physical Sign ◽  
Toxicological Evaluation ◽  
Toxicological Effect ◽  
Testicular Weight

Ruzu bitters black for men (RBBM) is a polyherbal product widely used amongst men in Nigeria to enhance libido, rejuvenate male organs and to manage erectile dysfunctions, prostate anomalies, weak erection, and premature ejaculation. This study was carried out to investigate the toxicological effect from the use of herbal product. Acute toxicity test of RBBM on rats was carried out in two phases; 10 mg/kg, 100 mg/kg and 1000 mg/kg for phase I and 1600mg/kg, 2900 mg/kg and 5000 mg/kg for phase II, were administered respectively. For sub-acute toxicity, two groups of 5 animals each received RBBM (0.87 mg/kg and1.17 mg/kg respectively) and a third group received water orally for 28 days. The study analyzed the median lethal dosage, and sperm morphology, sperm motility, sperm count, sperm viability and histology of the testes as indices for sub-acute toxicity. No death was recorded for the acute and sub-acute studies but there was a moderate physical sign of toxicity. In the sub-acute toxicity study, there was a significant increase (p˂0.05) in testicular weight of Group 1 animals. Also, sperm count, and sperm motility increases significantly (p˂0.05) while there was a decrease in multiple tail sperm across the test groups. RBBM is not toxic to sperm morphology and causes no death at 5000 mg/kg in male albino Wistar rats.

scholarly journals Reproductive & Biomarker Response to a Daily Dose of Instant Noodle Seasoning in Male Albino Rats (Rattus norvegicus)

2019 ◽  
pp. 1-8
Author(s):  
E. Oriakpono, Obemeata ◽  
C. Ibanibo, Blessing
Keyword(s):  
Sperm Count ◽  
Monosodium Glutamate ◽  
Treated Group ◽  
Albino Rats ◽  
Daily Consumption ◽  
Biomarker Response ◽  
Significant Difference ◽  
Average Control ◽  
The Mean ◽  
Instant Noodle

The effect of a daily consumption of Instant noodle seasoning containing the Monosodium glutamate (MSG) on rat was evaluated, The parameters investigated include; Alkaline aminotransferase (ALT), Aspartate aminotransferase  (AST). Hemoglobin (Hb), packed cell volume (PCV) white blood cell (WBC), protein, platelets, lymphocytes and Serum electrolytes; sodium (Na+), potassium (K+) chloride (Cl), bicarbonate (HCO3 - ). Sperm count was also investigated. The results revealed the following, the mean PCV was 29 and 25.13on week 1 and week 4, with an average control of 30.69, mean Hb was 10 in week 1 and 6.57 in week 4, RBC had an average control of 5.28 while week 1 had a mean of 4.77 and week 4 3.67, there was a significant difference (P<0.05) for PCV and Hb. The mean WBC and Lymphocyte were 6 and 61 in the first week, and 5.8 and 60.17 on the fourth week, with an average control of 5.28 for WBC and 77.53 for lymphocytes. Platelet had a mean of 251 on the first week and a mean of 532 on the fourth week with a significant difference across the group in WBC and platelets (P<0.05). The           mean serum Na, K and Cl reduced from 140.67, 4.13 and 100.67 in week 1 to 116, 2.5 and 98 in week 4 with a significant difference (P<0.05) across the group when compared to the average control for Na and K. HCO3 had a mean of 23.67 in week 1 and a mean of 22.67 in week 4 in the treated group. AST had a mean of 24 in week 1 which increased to 41.67 in week 4 while ALT increased from a mean of 4.00 in week 1 and 28 in week 4 with a significant difference (P<0.05) across the group. The mean serum protein was 51.93 in week 1 and a 74.29 in week 4. The           mean sperm count was 800, 299.67, 450.67 and 501 for week 1, 2, 3 and 4 respectively. The results indicates that daily consumption of Instant noodle seasoning may cause liver damage,            and kidney dysfunction and has been discovered to have negative effects on blood and sperm cells.

scholarly journals Improvement of Lead Acetate-Induced Testicular Injury and Sperm Quality Deterioration by Solanum Anomalum Thonn. Ex. Schumach Fruit Extracts in Albino Rats

2019 ◽  
Author(s):  
Samuel James Offor ◽  
Herbert Orji Mbagwu ◽  
Orish Ebere Orisakwe
Keyword(s):  
Lead Acetate ◽  
Sperm Quality ◽  
Sperm Count ◽  
Treated Group ◽  
Protective Role ◽  
Albino Rats ◽  
Acetate Solution ◽  
Group 4 ◽  
Group 2 ◽  
Testicular Injury

Objective: This study has investigated the protective role of a natural alternative, Solanum anomalum fruit extract in lead induced testicular toxicity in male albino rats. Materials and methods: Twenty-four mature male albino rats were used, divided into four groups of six rats per group. Group 1 (control rats) were given distilled water (10ml/kg), group 2 received lead acetate solution 60mg/kg, group 3 received lead acetate (60mg/kg) followed by Solanum anomalum (452mg/kg) and group 4 rats were given lead acetate (60mg/kg) followed by Solanum anomalum (678mg/kg) by oral gavage daily for 28 days. Results: Lead treated group showed significant increase in Malondialdehyde MDA (1.58 ± 0.09 to 1.90 ± 0.17 µmol/L of plasma) and decreases in Superoxide dismutase SOD, glutathione peroxidase (482.85 ± 53.43 to 247.18 ± 70.40 U/L of blood), total glutathione (1.11 ± 0.03 to 0.56 ± 0.31 ng/µL) (144.80 ± 7.00 and 122.39 ± 4.63 U/ml of blood), epididymal sperm reserve, testicular sperm count, % sperm motility and % sperm viability. Conclusion: Co-administration of Solanum anomalum significantly reversed the effect of lead with restoration of histoarchitecture of the testes. Solanum anomalum may be a protective modulator of lead -induced testicular injury.

Detection of germ cell genotoxic potential, of carbon disuiphide using sperm head shape abnormality test

1999 ◽  
Vol 18 (12) ◽  
pp. 731-734 ◽  
Author(s):  
S Kumar ◽  
K G Patel ◽  
A K Gautam ◽  
K Agarwal ◽  
B A Shah ◽  
...  
Keyword(s):  
Germ Cell ◽  
Carbon Disulphide ◽  
Sperm Count ◽  
Treated Group ◽  
Sperm Head ◽  
Adrenal Weight ◽  
Albino Rats ◽  
Head Shape ◽  
Genotoxic Potential ◽  
Group A

1 Adult male albino rats (CF Strain) were administered i.p. CS2 dissolved in cotton seed oil at doses of 25, 50, 100 and 200 mg/kg b.wt. for a period of 60 days. Effect of CS, on epididymis, adrenal weight, sperm count and sperm head shape abnormality was studied. 2 Epididymal weight remained unaltered in 25, 50 and 100 mg/kg CS2 treated groups, whereas in highest dose of CS2 treated (200 mg/kg) group a non-significant reduction in epididymis weight was observed. A slight increase in adrenal weight was observed in lower doses groups (25 and 50 mg/kg) while a considerable decrease in adrenal weight was noted in highest dose (200 mg/kg) of CS, treated group in the present study. 3 An increase in sperm head shape abnormality and decrease in sperm count was observed in all the CS2 treated groups. However, the changes were statistically significant only after higher dose of CS2 treatment as compared to control. 4 This study suggests that CS2 may have the potential to induce adverse effects on male reproductive system of rats. Sperm head shape abnormality assay used in this study also elicits germ cell genotoxic potential of carbon disulphide.

scholarly journals TESTICULAR MORPHOLOGY OF ALBINO RAT

2016 ◽  
Vol 23 (03) ◽  
pp. 343-349
Author(s):  
Muhammad Mazhar Hijazi ◽  
Muhammad Rashid Ahmed Ahmed ◽  
Hafiz Syed Imran Ul Haq ◽  
Syed Naeemul Hassan Naqvi ◽  
Rehana Parveen
Keyword(s):  
Control Level ◽  
Treated Group ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Albino Rat ◽  
Testicular Weight ◽  
Testicular Morphology ◽  
Androgenic Anabolic Steroid ◽  
Harmful Effects ◽  
Withdrawal Effects

Withdrawal effects of chronic androgenic anabolic steroid on hormonal andtesticular morphology were studied .Forty five male albino rats were divided into 1) Control 2)Chronic group and 3) Withdrawal group. Testoviron was injected at a dose of 400mg/kg bodyweight intramuscularly once in two weeks for 14 weeks, and then drug was withdrawan foranother 14 weeks. Testes were removed and fixed in 10% formalin and processed. Followingwithdrawal of AAS, testicular and relative testicular weight was restored to control. Increasedtubular count also returned near to normal while decreased diameter of seminiferous tubules,thickness of germinal epithelium, count and diameter of leydig cells were also restored nearto normal in withdrawal group when compared with the chronically treated group. Histologicalobservations also revealed that degenerated spermatogenic cells were returned to their normalappearance and oedematous vacuoles were reduced. Moreover, decreased level of reproductivehormones, i.e. FSH, LH and testosterone also returned to control level in withdrawal group.These results indicated that chronic AAS has substantial harmful effects on hormonal andtesticular morphology. However, these adverse effects gradually restored to normal followingwithdrawal from AAS.

scholarly journals Effects of aqueous leaf extract of Acalypha wilkesiana on semen morphology and characteristics in male Wistar albino rats

2020 ◽  
Vol 46 (2) ◽  
pp. 47-54
Author(s):  
J. O Olukunle ◽  
E. B. Jacobs ◽  
O. E. Adeleye ◽  
J. A. Oyewusi ◽  
O. T. Adenubi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Sperm Count ◽  
Experimental Period ◽  
Treated Group ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
High Dose ◽  
Aqueous Leaf Extract ◽  
Acalypha Wilkesiana ◽  
Wistar Albino Rats

To study the effect of the aqueous leaf extract of Acalypha wilkesiana (Euphorbiaceae) on some reproductive parameters of male Wistar albino rats, three groups (A, B and C) of six rats each were treated with 400, 800 and 1600 mg/kg/day of leaf extract of Acalypha wilkesiana, respectively for 28 days while 5 mLs/kg of distilled water was given to the controlgroup D. At the end of the experimental period, animals were sacrificed and sperm characteristics, histology of the testes and epididymis were assessed. Sperm motility (80.00+7.0, 75.00+2.9), sperm count (82.75+2.7, 76.50+3.8) and sperm live/dead (96.00+2.0, 94.50+1.7) were not significantly reduced (P<0.05) at the dosages of 400 and 800 mg/kg Acalypha wilkesiana leaf extract, respectively. did not have any significant reduction (P<0.05) in, but significant (P<0.05) reductions in sperm count (71.50+3.5*) was observed at 1600 mg/kg dose. Also, significant (P<0.05) increase in morphologicalabnormalities of the spermatozoa were only observed in the 1600 mg/kg treated group of rats. Histopathological evaluation of the testis and revealed varying degrees of degeneration and necrosis of the germinal epithelia cell of the seminiferous tubules and spermiostasis, whereas no significant lesion was seen in the epididymis. The high dose of the leaf extract of Acalypha wilkesiana may have significant anti-spermatogenic effects on adult male Wistar rats.

scholarly journals Effects of curcumin on sperm parameters abnormalities induced by morphine in rat

2017 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
S.H. Roshankhah ◽  
M.R. Salahshoor ◽  
S. Aryanfar ◽  
F. Jalili ◽  
M. Sohrabil ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Testosterone Level ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Treated Group ◽  
Sperm Parameters ◽  
Male Rats ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group

Opioids are the most potent and effective analgesics available and have become accepted as appropriate treatment for acute, cancer and non-cancer. Morphine, which is commonly used for the treatment of severe pain, gastrointestinal tract and kidneys. Curcumin petals consist of, glycosides, flavonoids, and anthocyanin. The study aims at evaluating curcumin effect and morphine on sperm parameters, testis tissue and serum testosterone level in rat. In this experimental study, 48 male rats with 28 weeks of age and limited weight of 270 to 300g were selected. They were divided into eight groups of 6, untreated control group; morphine – treated group (20 mg/kg/day); curcumin -treated groups (10, 30, 60 mg/kg/day); and morphine and curcumin treated group intraperitoneal administration for successive 28 days. After 24hours animals were killed. Sperm motility was measured using WHO protocols. The sperm parameter such as motility, sperm count, morphology, seminiferous tubules diameter, weight testis, and serum testosterone level were analyzed (oneway ANOVA). Curcumin (10, 30 and 60 mg/kg) significantly increased mean percentage of sperm motility, count, testis weight, and serum testosterone level compared to control group (p<0.05). Testosterone level decreased significantly in rats treated with morphine. Co-administration of curcumin to morphine-treated rats improved the histopathological alterations induced by morphine in testis and increased the sperm count. Curcumin has a very strong antioxidant effects at applied doses and it can probably be used as an antioxidant and food supplement in reproductive disorders.Journal of Medical and Biomedical Sciences (2017) 6(2), 1-10

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