scholarly journals Phytochemical Screening and Antioxidant Activity Test of Red Onion (Allium ascolinicum L.) Extract Variety of Palu Valley

2020 ◽  
Vol 9 (2) ◽  
pp. 63-69
Author(s):  
Rimah Juwita ◽  
Daud K. Walanda ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Phytochemical Screening ◽  
Onion Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Qualitative Test ◽  
Central Sulawesi ◽  
Positive Results

The red onion of Palu valley variety is a plant that is widely found in Palu area and regarded as a leading commodity in Central Sulawesi. The purpose of this study was to examine the secondary metabolite compounds contained in red onion (Allium ascolinicum L.) of Palu valley variety and its antioxidant activity. Antioxidant activity test in this study used DPPH (1,1-Diphenyl-2-Pikrilhidrazil) as a source of free radical, and vitamin C as the comparison. The antioxidant activity of red onion extract was analyzed using a UV-Vis spectrophotometer. The red onion tuber powder was extracted by maceration using ethanol solvent. Qualitative test of onion powder showed positive results on alkaloids, flavonoids, and tannin compounds. The samples and vitamin C were tested for antioxidant activity in various extract concentrations of 10, 30, 50, 70, and 90 ppm. The results showed that red onion extract of Palu valley variety presented an IC50 value of 38.548 ppm, and vitamin C presented an IC50 value of 22.646 ppm. Based on these values, the red onion (Allium ascolinicum L.) extract is categorized as a very strong class of antioxidant which can be used as a source of natural antioxidant.

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Kluwih (Artocarpus Communis)

2018 ◽  
Vol 7 (2) ◽  
pp. 85
Author(s):  
Mohammad Arif ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Vitamin C ◽  
Positive Control ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ic50 Value ◽  
Fruit Powder ◽  
Central Sulawesi

This study aimed to determine antioxidant activity of artocarpus camansi fruit extracts from Sigi, Central Sulawesi. This study was conducted by maceration extraction technique, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) as the source of free radicals, and vitamin C as a positive control. UV-Vis spectrophotometer was used for measuring the absorbance of artocarpus camansi fruit extracts. artocarpus camansi fruit powder was extracted by maceration using ethanol. Various concentrations of artocarpus camansi fruit extracts used were 20, 40, 60, and 80 mg/L. The results showed that the IC50 value for artocarpus camansi (artocarpus communis) fruit extract was 88.715 mg/L, whereas the IC50 value for vitamin C was 37.153 mg/L. Artocarpus camansi (artocarpus communis) fruit extracts are powerful antioxidants category based on IC50 value. The optimum percentage antioxidant activity of artocarpus camansi fruit extracts to inhibit free radical amounted to by 50.65%

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals ANTIOXIDANT ACTIVITY TEST of SIJUKKOT (Lactuca indica L.) LEAVES EXTRACT LEAF

2020 ◽  
Vol 3 (2) ◽  
pp. 49
Author(s):  
Sumiati Nainggolan ◽  
Ida Duma Riris
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
A Value ◽  
Activity Test ◽  
Radical Method ◽  
Standard Of Comparison ◽  
Lactuca Indica

Has been investigated the antioxidant activity of  sijukkot ( Lactuca indica L.) The antioxidant activity was tested by capturing 2,2’-diphenyl-picrylhydrazyl (DPPH) free radical method and Vitamin C as a standard of comparison, to absorption using UV-Vis  spectrophotometry at a wavelength of 517nm. The data obtained were analyzed using the SPSS for windows, resulting in a value of y = 0.2036x + 30.35 for sijukkot extract and y = 29.625x - 73.664 for vitamin C. It was found the leaves have an antioxidant site with 96,51 ppm through IC50 test 4,17 ppm which used Vitamin C as a control. 

AKTIVITAS ANTIOKSIDAN EKSTRAK UMBI SUWEG (Amorphophallus paeoniifolius) DARI BERBAGAI TINGKAT POLARITAS PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Deddy Firman ◽  
Nurhaeni Nurhaeni ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Tuber Type

ABSTRACT Has done research about antioxidant activity of suweg tuber (Amorphophallus campamulatus) based on the level of polar solvent. This research begins with in phased maceration extraction using n-hexane, ethyl acetate dan ethanol. Next step was antioxidant activity test with tyocyanate to n-hexane extract and DPPH methods to ethanol extract, ethyl acetate and vitamin C. The result showed antioxidant activity in n-hexane extract i.e. 14,96 %, end 77,13 % BHT while in ethyl acetate, ethanol and vitamin C reached IC50 value are 458,102 ppm, 223,268 ppm dan 26,76 ppm. Keywords: Suweg tuber, type of solvent, maceration, antioxidant

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antioksidan dari Daun Nasi (Phrynium capitatum) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 11 ◽  
Author(s):  
Dwi Putra Wijaya ◽  
Jessy E. Paendong ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dpph Solution

Telah dilakukan penelitian tentang skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Skrining fitokimia meliputi pengujian alkaloid, flavonoid, saponin, steroid, triterpenoid dan fenolik. Uji aktivitas antioksidan dilakukan dengan penambahan 2 mL larutan DPPH dengan 0,5 mL larutan ekstrak dengan konsentrasi masing-masing 1000, 750, 500 dan 250 µg/mL. Data yang diproleh kemudian digunakan untuk menghitung nilai IC50 dari ekstrak etanol daun nasi. Hasil pengujian menunjukan bahwa ekstrak etanol daun nasi mengandung senyawa alkaloid, flavonoid, saponin, steroid dan fenolik. Ekstrak etanol daun nasi memiliki aktivitas antioksidan trrtinggi pada konsentrasi 1000 µg/mL sebesar 65,77 % dan semakin menurun aktivitasnya dengan berkurangnya konsentrasi ekstrak, konsentrasi 750, 500 dan 250 µg/mL masing-masing sebesar 53,96 %, 43,8 % dan 19,58 % dengan nilai IC50 pada konsentrasi 701,78 µg/mL.A research on phytochemicals screening and antioxidant activity test of daun nasi (Phrynium capitatum) using DPPH (1,1-diphenyl-2-picrylhydrazyl) method had been conducted. Test for alkaloids, flavonoids, saponins, steroids, triterpenoids and phenolic were included in phytochemical screening. Antioxidant activity was tested through the addition of 2 mL DPPH solution into 0.5 mL of the extract having a concentration of 1000, 750, 500 and 250 µg/mL respectively. The data obtained was used to calculate the IC50 value for the ethanol extract of daun nasi. The results showed that the ethanol extract of daun nasi contains alkaloids, flavonoids, saponins, steroids and phenolic. Ethanol extract of daun nasi has the highest antioxidant activity of 65.77% at concentration of 1000 µg/mL. Its activity decreased with decreasing concentration of the extract, which were 53.96 %, 43.8 % and 19.58 % at concentration of 750, 500 and 250 µg/mL respectively. IC50 values was found at a concentration of 701.78 µg/mL.

scholarly journals Pengaruh Metode Pengeringan Terhadap Aktivitas Antioksidan Ekstrak Etanol

2019 ◽  
Vol 3 (1) ◽  
pp. 44-52
Author(s):  
Endra Pujiastuti ◽  
Rahma Sani Saputri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethanol Extract ◽  
Drying Methods ◽  
Drying Method ◽  
Oven Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Qualitative Test ◽  
High Antioxidant Activity

Drying is the most important step to keep the compound stability in simplicia and is very influential to produce good quality igredients in the antioxidant activity contained in the simplicia. Antioxidants are oxidation-free inhibitors of free radical reactions. Parijoto is one of the plants that have antioxidant compounds.This study aims to determine the effect of drying, concentration of antioxidant activity of parijoto (Medinilla speciosa Blume) fruit. The drying method used is direct sunlight drying, indirect sunlight and oven. The antioxidant activity of ethanol extract on drying method was determined by 2,2 diphenyl-1-picrylhidrazyl (DPPH) method which is free radical. The qualitative test DPPH showed that the higher levels of ethanol extract from the three drying methods further diminished the color of DPPH solution in ethanol.The result showed thet the drying method gave different effect to the antioxidant activity of parijoto fruit which is significant(p0,05). Drying methods wich have high antioxidant activity are given by drying using oven method with an IC50 value 33,75µg/ml.

scholarly journals Antioxidant Activity Test of 2,6-bis-(2’-furilidyn)-Cyclohexanone, ; 2,5-bis-(2’-furilidyn)-Cyclopentanone; 1,5-Difuryl-1,4-pentadien-3-one

2010 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Ismi Rahmawati ◽  
Endang Sri Rejeki ◽  
Sardjiman Sardjiman
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Positive Control ◽  
Log P ◽  
Dpph Radical ◽  
Curcumin Analog ◽  
Activity Test ◽  
Activity Structure ◽  
Ic50 Value

Antioxidant is an essential compound to keep man’s health due to its function as radical scavenging. Curcumin analog compounds can function as antioxidant (Sardjiman, 2000). The aim of the experiment was to find out the antioxidant activity of 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one compounds, and the antioxidant activity of each compound against DPPH  radical with IC50 parameter as well as the correlation of compounds structure’s activities against antioxidant. The antioxidant activity of curcumin analog compounds wereas tested against DPPH free radical. The test was conducted in 5 series of concentrations by adding 4.0 ml test solutions with 1.0 ml DPPH. The antioxidant activity against free radical was measured usingwith spectrophotometer at 517 nm wavelength and determined for the IC50 value. The experiment employed rutin as positive control. The result of the experiment showed that curcumin analog compounds have antioxidant activity with IC50 of rutin, 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one as follows: 4.93 ppm, 22.73 ppm, 20.67 ppm, and 18.80 ppm  respectively. The highest antioxidant activity belonged to 1,.5-difuril-1.4-pentadien-3-one compound which is 18.80 ppm . Correlation of activity structure of the 3 compounds can be seen from the log p parameter and energy space of HOMO-LUMO.Key words: Antioxidant, DPPH, Analog Curcumin

scholarly journals Antioxidant Activity Tests of Extract of Principle Crafts (Phaleria macrocarpa)

2020 ◽  
Vol 8 (3) ◽  
pp. 125-129
Author(s):  
Tri Putri ◽  
Anang W. M. Diah ◽  
Afadil Afadil
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Positive Control ◽  
Degenerative Diseases ◽  
Fruit Extract ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phaleria Macrocarpa ◽  
Central Sulawesi

Phaleria macrocarpa contains secondary metabolite compounds such as alkaloids, terpenoids, saponins, polyphenols, tannins, sterols, and coumarins. Those metabolites are antioxidants and can be efficacious in the healing of various degenerative diseases such as cervical cancer and diabetes. This plant is widely grown in Tindaki Village, Parigi Mautong, Central Sulawesi. The aim of this study was to determine the antioxidant power of those phaleria macrocarpa fruit extract. This study was conducted by using maceration extraction technique with ethanol as solvent, and compound 2,2-difenil-1-picrilhidrazil (DPPH) as source of free radical, and vitamin C as the positive control. The UV-Vis spectrophotometer was used to measure the absorbance of the extract. Various concentrations of the extract used were 5 ppm, 15 ppm, 25 ppm, and 35 ppm. The results showed that the IC50 value of the fruit extract was 28.242 ppm, while the IC50 value of vitamin C was 19.302 ppm. These IC50 values show that vitamin C was a stronger antioxidant activity than the fruit extract, but the fruit extract was still as a very strong natural antioxidant category.

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

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