PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

scholarly journals Phytochemical Screening and Antioxidant Activity Test of Red Onion (Allium ascolinicum L.) Extract Variety of Palu Valley

2020 ◽  
Vol 9 (2) ◽  
pp. 63-69
Author(s):  
Rimah Juwita ◽  
Daud K. Walanda ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Phytochemical Screening ◽  
Onion Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Qualitative Test ◽  
Central Sulawesi ◽  
Positive Results

The red onion of Palu valley variety is a plant that is widely found in Palu area and regarded as a leading commodity in Central Sulawesi. The purpose of this study was to examine the secondary metabolite compounds contained in red onion (Allium ascolinicum L.) of Palu valley variety and its antioxidant activity. Antioxidant activity test in this study used DPPH (1,1-Diphenyl-2-Pikrilhidrazil) as a source of free radical, and vitamin C as the comparison. The antioxidant activity of red onion extract was analyzed using a UV-Vis spectrophotometer. The red onion tuber powder was extracted by maceration using ethanol solvent. Qualitative test of onion powder showed positive results on alkaloids, flavonoids, and tannin compounds. The samples and vitamin C were tested for antioxidant activity in various extract concentrations of 10, 30, 50, 70, and 90 ppm. The results showed that red onion extract of Palu valley variety presented an IC50 value of 38.548 ppm, and vitamin C presented an IC50 value of 22.646 ppm. Based on these values, the red onion (Allium ascolinicum L.) extract is categorized as a very strong class of antioxidant which can be used as a source of natural antioxidant.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals ANTIOXIDANT ACTIVITY TEST of SIJUKKOT (Lactuca indica L.) LEAVES EXTRACT LEAF

2020 ◽  
Vol 3 (2) ◽  
pp. 49
Author(s):  
Sumiati Nainggolan ◽  
Ida Duma Riris
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
A Value ◽  
Activity Test ◽  
Radical Method ◽  
Standard Of Comparison ◽  
Lactuca Indica

Has been investigated the antioxidant activity of  sijukkot ( Lactuca indica L.) The antioxidant activity was tested by capturing 2,2’-diphenyl-picrylhydrazyl (DPPH) free radical method and Vitamin C as a standard of comparison, to absorption using UV-Vis  spectrophotometry at a wavelength of 517nm. The data obtained were analyzed using the SPSS for windows, resulting in a value of y = 0.2036x + 30.35 for sijukkot extract and y = 29.625x - 73.664 for vitamin C. It was found the leaves have an antioxidant site with 96,51 ppm through IC50 test 4,17 ppm which used Vitamin C as a control. 

AKTIVITAS ANTIOKSIDAN EKSTRAK UMBI SUWEG (Amorphophallus paeoniifolius) DARI BERBAGAI TINGKAT POLARITAS PELARUT

KOVALEN ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Deddy Firman ◽  
Nurhaeni Nurhaeni ◽  
Ahmad Ridhay
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Activity Test ◽  
Ic50 Value ◽  
Tuber Type

ABSTRACT Has done research about antioxidant activity of suweg tuber (Amorphophallus campamulatus) based on the level of polar solvent. This research begins with in phased maceration extraction using n-hexane, ethyl acetate dan ethanol. Next step was antioxidant activity test with tyocyanate to n-hexane extract and DPPH methods to ethanol extract, ethyl acetate and vitamin C. The result showed antioxidant activity in n-hexane extract i.e. 14,96 %, end 77,13 % BHT while in ethyl acetate, ethanol and vitamin C reached IC50 value are 458,102 ppm, 223,268 ppm dan 26,76 ppm. Keywords: Suweg tuber, type of solvent, maceration, antioxidant

Assesment Of Antioxidant Activity Test Of KERSEN Leaf (Muntingia calabura L.) And Epiphyte with DPPH (2.2-Diphenyl-1-Picrylhidrazyl)

2020 ◽  
Vol 1 (2) ◽  
pp. 60-66
Author(s):  
Nurlutfiyyah Aini ◽  
Fatmawati ◽  
Nita Parisa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Linear Regression Analysis ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Human Needs ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Free Radical Activity ◽  
Muntingia Calabura

Antioxidant is very important to give protection against free radical activity and highlyreactive molecules that could lead in slowing the progression of degenerative disease.In case of degenerative disease, internal antioxidant cannot neutralize the increasingconcentration of free radical. Because of that, human needs external antioxidant.Kersen (Muntingia calabura L.) is a plant that is known for its antioxidant content.Plants containing antioxidant experience is kersen (Muntingia calabura L.). Researchstudy to determine the antioxidant activity of Kersen plant and knows the differenceof antioxidant activity, based on the process of extract and infusion. Research wasdone by experimental study which was oriented in testing antioxidant activity in(Morinda citrifolia L.) extract and infusion. Extraction was done by using 96% ethanolas solvent, meanwhile infusion was made by using aquadest. Extract and infusionwas divided into group of concentration and antioxidant activity was tested byDPPH(2,2-Diphenyl-1-Picrylhidrazyl) method by measuring the absorbance usingspectrophotometer at 520 nm wavelength. Percentage of DPPH inhibition and IC50then analyzed using linear regression analysis. Ethanolic extract of kersen leaf andepiphyte had IC50 value of 113,801 ppm and 98,7802 ppm, respectively. Kersen leafinfusion showed 191,7624 ppm IC50 values, besides its epiphyte had 131,6750 ppm.Antioxidant activity of Muntingia calabura L. in the order from kersen leaf anepiphyte, and epiphyte extract has a higher antioxidant content than others.

scholarly journals Antioxidant Activity Test of 2,6-bis-(2’-furilidyn)-Cyclohexanone, ; 2,5-bis-(2’-furilidyn)-Cyclopentanone; 1,5-Difuryl-1,4-pentadien-3-one

2010 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Ismi Rahmawati ◽  
Endang Sri Rejeki ◽  
Sardjiman Sardjiman
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Positive Control ◽  
Log P ◽  
Dpph Radical ◽  
Curcumin Analog ◽  
Activity Test ◽  
Activity Structure ◽  
Ic50 Value

Antioxidant is an essential compound to keep man’s health due to its function as radical scavenging. Curcumin analog compounds can function as antioxidant (Sardjiman, 2000). The aim of the experiment was to find out the antioxidant activity of 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one compounds, and the antioxidant activity of each compound against DPPH  radical with IC50 parameter as well as the correlation of compounds structure’s activities against antioxidant. The antioxidant activity of curcumin analog compounds wereas tested against DPPH free radical. The test was conducted in 5 series of concentrations by adding 4.0 ml test solutions with 1.0 ml DPPH. The antioxidant activity against free radical was measured usingwith spectrophotometer at 517 nm wavelength and determined for the IC50 value. The experiment employed rutin as positive control. The result of the experiment showed that curcumin analog compounds have antioxidant activity with IC50 of rutin, 2,.6-bis-(2’-furilidin)-cyclohexanone, 2,.5-bis-(2’furilidin)-cyclopentanone, and 1,.5-difuril-1.4-pentadien-3-one as follows: 4.93 ppm, 22.73 ppm, 20.67 ppm, and 18.80 ppm  respectively. The highest antioxidant activity belonged to 1,.5-difuril-1.4-pentadien-3-one compound which is 18.80 ppm . Correlation of activity structure of the 3 compounds can be seen from the log p parameter and energy space of HOMO-LUMO.Key words: Antioxidant, DPPH, Analog Curcumin

scholarly journals Uji Aktivitas Antioksidan Ekstrak Buah Kluwih (Artocarpus Communis)

2018 ◽  
Vol 7 (2) ◽  
pp. 85
Author(s):  
Mohammad Arif ◽  
Nurdin Rahman ◽  
Supriadi Supriadi
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Vitamin C ◽  
Positive Control ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ic50 Value ◽  
Fruit Powder ◽  
Central Sulawesi

This study aimed to determine antioxidant activity of artocarpus camansi fruit extracts from Sigi, Central Sulawesi. This study was conducted by maceration extraction technique, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) as the source of free radicals, and vitamin C as a positive control. UV-Vis spectrophotometer was used for measuring the absorbance of artocarpus camansi fruit extracts. artocarpus camansi fruit powder was extracted by maceration using ethanol. Various concentrations of artocarpus camansi fruit extracts used were 20, 40, 60, and 80 mg/L. The results showed that the IC50 value for artocarpus camansi (artocarpus communis) fruit extract was 88.715 mg/L, whereas the IC50 value for vitamin C was 37.153 mg/L. Artocarpus camansi (artocarpus communis) fruit extracts are powerful antioxidants category based on IC50 value. The optimum percentage antioxidant activity of artocarpus camansi fruit extracts to inhibit free radical amounted to by 50.65%

scholarly journals ANTIOXIDANT ACTIVITY OF TAUCO ETHANOL EXTRACT AND ITS FRACTIONS

2017 ◽  
Vol 10 (14) ◽  
pp. 57
Author(s):  
Resmi Mustarichie ◽  
Sandra Megantara ◽  
Wiwiek Indriyati ◽  
Ade Zuchrotun
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Extraction Methods ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Modified Method ◽  
Ic50 Value

Objective: To investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate and n-hexane from tauco.Methods: Two types of tauco was extracted using soxhletation methods, followed by fractionation using liquid-liquid extraction methods and phytochemical screening. Antioxidant activity test was carried out using DPPH with Ascorbic acid (vitamin C) as a reference. Modified method of Farnsworth was applied for phytochemical screening.Results: It was found that extracts of ethanol and ethyl acetate fraction containing flavonoids, monoterpenoid, and sesquiterpenoids whereas the water fraction and a fraction of n-hexane only contain monoterpenes and sesquiterpenoids. The IC50 value for the ethanol extract, water, ethyl acetate, and n-hexane fractions of two taucos in a row were 1192.71 ppm, 1746.01 ppm, 722.38 ppm, 1845.45 ppm and 1190,15 ppm, 1740.30, 710.46, for tauco A and B respectively.Conclusion: It was unexpected that tauco ethanol extract and fractions showed much weaker antioxidant activity than vitamin C, which had the IC50 value of 4.41 ppm. 

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Antioxidant Activity Test of Ethanol and Water Extracts of Celery (Apium graveolens L.)

2020 ◽  
Vol 9 (2) ◽  
pp. 93-101
Author(s):  
Nurmiati Nurmiati ◽  
Siti Nuryanti ◽  
Tahril Tahril
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Water Extract ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Apium Graveolens ◽  
Antioxidant Power ◽  
Water Extracts ◽  
Activity Test ◽  
Ic50 Value

This study aimed to determine the antioxidant activity of ethanol and water extracts of celery (Apium graveolens L.). Antioxidant activity test in this study used DPPH (2,2-diphenyl-1-picrylhydrazine) as a source of free radicals and vitamin C as a positive control. The antioxidant activity of celery extracts was analyzed using a UV-VIS spectrophotometer. Celery powder was extracted by firstly the maceration method using different solvents (ethanol and water) and secondly with the decoction method using water solvents. Celery extract was tested for antioxidant activity with various concentrations of 20, 40, 60, and 80 ppm. The results obtained that water extract (maceration) had a very strong antioxidant power with 23.713 ppm of IC50 values, for ethanol extract (maceration) had a strong antioxidant power with 59.492 ppm of IC50 value, water extract (dekok) had also a very strong antioxidant with 77.446 ppm of IC50 value, and vitamin C had a very strong antioxidant power with 15.631 ppm of an IC50 value. Based on the IC50 value obtained, the water extract by the maceration method was very good to be used as a source of natural antioxidants, because it had a very strong antioxidant activity value which was equivalent to vitamin C.

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