Effects of high temperature on rice grain development and quality formation based on proteomics comparative analysis under field warming

With the intensification of global warming, rice production is facing new challenges. Field evidence indicates that elevated temperature during rice grain-filling leads to the further deterioration of grain quality. In order to clarify the potential regulatory mechanism of elevated temperature on the formation of rice quality, the DIA mass spectrometry method under the background of field warming was conducted to investigate the regulatory effects of high temperature on grain development and material accumulation pathways. The results showed that a total of 840 differentially expressed proteins were identified during the grain-filling process under elevated temperature. These differentially expressed proteins participated in carbon metabolism, amino acid biosynthesis, signal transduction, protein synthesis, and alternately affected the material accumulation of rice grains. The significant up-regulation of PPROL 14E, PSB28, granule-bound starch synthase I, and the significant down-regulation of 26.7 kDa heat shock protein would lead to the component difference in grain starch and storage proteins, and that could be responsible for the degradation of rice quality under elevated temperature. Results suggested that proteins specifically expressed under elevated temperature could be the key candidates for elucidating the potential regulatory mechanism of warming on rice development and quality formation. In-depth study on the metabolism of storage compounds would be contributed in further proposing high-quality cultivation control measures suitable for climate warming.

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Genome Wide Analysis of the Transcriptional Profiles in Different Regions of the Developing Rice Grains

Rice ◽

10.1186/s12284-020-00421-4 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Ting-Ying Wu ◽

Marlen Müller ◽

Wilhelm Gruissem ◽

Navreet K. Bhullar

Keyword(s):

Gene Expression ◽

Dna Sequence ◽

Expression Patterns ◽

Grain Filling ◽

Aleurone Layer ◽

Rice Grain ◽

Sequence Motifs ◽

Grain Development ◽

Spatio Temporal ◽

Dna Sequence Motifs

Abstract Background Rice is an important food source for humans worldwide. Because of its nutritional and agricultural significance, a number of studies addressed various aspects of rice grain development and grain filling. Nevertheless, the molecular processes underlying grain filling and development, and in particular the contributions of different grain tissues to these processes, are not understood. Main Text Using RNA-sequencing, we profiled gene expression activity in grain tissues comprised of cross cells (CC), the nucellar epidermis (NE), ovular vascular trace (OVT), endosperm (EN) and the aleurone layer (AL). These tissues were dissected using laser capture microdissection (LCM) at three distinct grain development stages. The mRNA expression datasets offer comprehensive and new insights into the gene expression patterns in different rice grain tissues and their contributions to grain development. Comparative analysis of the different tissues revealed their similar and/or unique functions, as well as the spatio-temporal regulation of common and tissue-specific genes. The expression patterns of genes encoding hormones and transporters indicate an important role of the OVT tissue in metabolite transport during grain development. Gene co-expression network prediction on OVT-specific genes identified several distinct and common development-specific transcription factors. Further analysis of enriched DNA sequence motifs proximal to OVT-specific genes revealed known and novel DNA sequence motifs relevant to rice grain development. Conclusion Together, the dataset of gene expression in rice grain tissues is a novel and useful resource for further work to dissect the molecular and metabolic processes during rice grain development.

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RNA Sequencing Reveals Rice Genes Involved in Male Reproductive Development under Temperature Alteration

Plants ◽

10.3390/plants10040663 ◽

2021 ◽

Vol 10 (4) ◽

pp. 663

Author(s):

Sudthana Khlaimongkhon ◽

Sriprapai Chakhonkaen ◽

Keasinee Tongmark ◽

Numphet Sangarwut ◽

Natjaree Panyawut ◽

...

Keyword(s):

High Temperature ◽

Rna Sequencing ◽

Reproductive Development ◽

Yield Reduction ◽

World Population ◽

Rice Grain ◽

Wild Type ◽

Rice Plants ◽

Male Development ◽

Temperature Conditions

Rice (Oryza sativa L.) is one of the most important food crops, providing food for nearly half of the world population. Rice grain yields are affected by temperature changes. Temperature stresses, both low and high, affect male reproductive development, resulting in yield reduction. Thermosensitive genic male sterility (TGMS) rice is sterile at high temperature and fertile at low temperature conditions, facilitating hybrid production, and is a good model to study effects of temperatures on male development. Semithin sections of the anthers of a TGMS rice line under low (fertile) and high (sterile) temperature conditions showed differences starting from the dyad stage, suggesting that genes involved in male development play a role during postmeiotic microspore development. Using RNA sequencing (RNA-Seq), transcriptional profiling of TGMS rice panicles at the dyad stage revealed 232 genes showing differential expression (DEGs) in a sterile, compared to a fertile, condition. Using qRT-PCR to study expression of 20 selected DEGs using panicles of TGMS and wild type rice plants grown under low and high temperature conditions, revealed that six out of the 20 selected genes may be unique to TGMS, while the other 14 genes showed common responses to temperatures in both TGMS and wild-type rice plants. The results presented here would be useful for further investigation into molecular mechanisms controlling TGMS and rice responses to temperature alteration.

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Impact of High Temperature Stress in Rice

International Journal of Economic Plants ◽

10.23910/2/2020.0366 ◽

2020 ◽

Vol 7 (3) ◽

pp. 108-110

Author(s):

E. Umarani ◽

◽

V. Hemalatha ◽

A. Saritha ◽

A. V. Ramanjaneyulu ◽

...

Keyword(s):

High Temperature ◽

High Temperatures ◽

Early Morning ◽

High Temperature Stress ◽

Oryza Glaberrima ◽

Rice Grain ◽

Irreversible Damage ◽

Peak Period ◽

Genetic Source ◽

The Tropics

Global warming has become a big concern in the world and it has been continuously increasing in recent decades due to the greenhouse effect. The increase in temperature has been striking and it can cause irreversible damage to plant growth and development. Though rice originates from the tropics, high temperatures of more than 35 °C during the reproductive stages reduces rice production, especially when the rice plant flowers when the high temperature occurs because it causes low seed setting and low yield. It was found that rice grain yield declined by 10% for each 1 °C increase in growing-season minimum temperature. Early-morning flowering is an important criteria in rice which escapes plant from high temperatures during peak period. It was found that Oryza glaberrima, a wild species of rice, is a useful genetic source since it has a habit of early-morning flowering and high transpiration with sufficient water, both of which are convenient traits for avoiding heat stress.

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Transcriptomic and metabolic flux analyses reveal shift of metabolic patterns during rice grain development

BMC Systems Biology ◽

10.1186/s12918-018-0574-x ◽

2018 ◽

Vol 12 (S4) ◽

Cited By ~ 1

Author(s):

Fangzhou Shen ◽

Xueting Wu ◽

Luoxi Shi ◽

Hang Zhang ◽

Yangmin Chen ◽

...

Keyword(s):

Metabolic Flux ◽

Rice Grain ◽

Grain Development

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Physiological and Yield Responses of Some Selected Rapeseed/ Mustard Genotypes to High Temperature Stress

Bangladesh Agronomy Journal ◽

10.3329/baj.v22i1.44936 ◽

2020 ◽

Vol 22 (1) ◽

pp. 47-56

Author(s):

Faruque Ahmed ◽

IM Ahmed ◽

N Mokarroma ◽

F Begum ◽

A Jahan

Keyword(s):

Antioxidant Activity ◽

High Temperature ◽

Seed Yield ◽

Dry Matter ◽

High Temperature Stress ◽

Development Stage ◽

Grain Development ◽

Leaf Chlorophyll ◽

Matter Production ◽

Sowing Dates

A pot experiment was conducted with five selected rapeseed/mustard genotypes (BJDH-11, BJDH-12, BJDH-20, BARI Sarisha-14, and BARI Sarisha-16) under two sowing dates (November 20 and December 20) for evaluating their responses to sowing date induced high temperature stress during rabi season of 2017-18. Sowing dates induced temperature variability showed remarkable changes in pheonlogy, leaf area, leaf chlorophyll content, dry matter production and seed yield. Although December 20 sown crop received lower temperatures (minimum 9.8 to 13.2 and maximum 22.6 to 27oC) than November 20 sown crop (minimum 14.8 to 16.4 and maximum 21 to 27.2oC) at flowering but reverse was found at grain development stage. Grain development stage of November 20sown crop received lower temperatures (minimum 8.2 to 13.2 and maximum 24.1 to 27 oC) while December 20 sown crop received higher temperatures at grain development stage (minimum 8.2 to 18 and maximum 22.6 to 32.5oC).As a result December 20 sown crop matured earlier (6 to 9 days) than November 20 sown crop. Leaf area/plant was higher in December 20 sown crops compared to November 20 sown but total dry matter production was more or less same in both the sowing dates. Leaf chlorophyll content did not show any remarkable variation due to variation in sowing dates. However, antioxidant activity like Catalyse (CAT), Peroxidase (POD) Ascorbate peroxidase (APX) and Malondial dehyde (MDA) were found higher in December 20 sown crops than that of November 20sown. Higher activity of APX, POD and CAT with lower activity of MDA indicates comparatively high temperature tolerant genotype. Among the genotypes APX, POD and CAT activity were found higher with lower activity of MDA in BJDH-11 and BJDH-20 and these genotypes also gave higher yield than others. On the basis of growth parameters, antioxidant activity and seed yield of genotype BJDH-11 and BJDH-20 could be select as terminal high temperature tolerance genotypes. Bangladesh Agron. J. 2019, 22(1): 47-56

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Comparative Analysis of ENDF, JEF and JENDL Data Libraries by Modelling High Temperature Reactors and Plutonium Based Fuel Cycles

Journal of Nuclear Science and Technology ◽

10.1080/00223131.2002.10875309 ◽

2002 ◽

Vol 39 (sup2) ◽

pp. 1167-1170 ◽

Cited By ~ 5

Author(s):

Danas Ridikas ◽

Laurent Bletzacker ◽

Olivier Deruelle ◽

Mansour Fadil ◽

Gabriele Fioni ◽

...

Keyword(s):

Comparative Analysis ◽

High Temperature ◽

Fuel Cycles ◽

High Temperature Reactors ◽

Data Libraries

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The tolerance of wheat to hight temperatures during reproductive growth. 2. Grain development

Australian Journal of Agricultural Research ◽

10.1071/ar9890015 ◽

1989 ◽

Vol 40 (1) ◽

pp. 15 ◽

Cited By ~ 68

Author(s):

IF Wardlaw ◽

IA Dawson ◽

P Munibi

Keyword(s):

Genetic Variation ◽

High Temperature ◽

Dry Weight ◽

Grain Development ◽

High Radiation ◽

Grain Number ◽

Temperature Conditions ◽

Specific Source ◽

Parental Lines ◽

Low Sensitivity

A study was undertaken in the Canberra phytotron of the response of 66 cultivars of wheat to high temperature (30/25�C) imposed throughout the period of grain development. The results from 20 Australian cultivars and their parental lines showed half of these to be clustered in the range where grain dry weight was reduced by 30-35% in response to high temperature when compared with 18/13�C controls. The non-Australian lines were more normally distributed in their response to high temperature and included some cultivars that were less sensitive to high temperature than the best of the Australian lines. It was concluded that there is a need for a wider assessment of genetic variation if a response to high temperature is to be incorporated in a breeding programme. A more specific study on Kalyansona, one of the better cultivars under high temperature conditions and high radiation levels, failed to reveal a specific source of temperature insensitivity in the background of Kalyansona. A comparison of the response to high temperature during grain development of twoF1 hybrids with their parental lines (Banks, Kalyansona and Pinnacle) provided some evidence that traits such as high grain number per ear, large grains and low sensitivity to high temperature could be effectively combined to produce a high grain weight per ear under high temperature conditions.

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