scholarly journals Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan

2021 ◽  
pp. 40-40
Author(s):  
Cansu Akbulut
Keyword(s):  
Light Microscope ◽  
Morphological Changes ◽  
Ovarian Tissue ◽  
Environmental Pollutants ◽  
Reproductive Potential ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Apoptotic Cells ◽  
Zona Radiata

Triclosan, produced as a broad-spectrum antibiotic in the early 1960s, is generally used as a preservative in personal care products, fabrics, plastic products such as kitchenware and toys. As a result of the high demand for triclosan, this chemical threatens the aquatic ecosystem by contaminating wastewater sources. Environmental pollutants affect the reproductive potential of fish, one of the most critical aquatic organisms. This study aimed to investigate the histopathological and apoptotic effects of triclosan in zebrafish gonads. Fish were exposed to sublethal concentrations of triclosan for 5 days, and general histological methods were applied. Histological sections were examined under a light microscope after staining with hematoxylin and eosin and toluidine blue. Triclosan exposure caused deterioration in ovarian tissue, such as shrinkage in the ooplasm, accumulation of proteinaceous fluid in the interstitial tissue, morphological changes of oocyte and the zona radiata. In testicular tissue, triclosan exposure caused fusion in seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, edema in seminiferous tubules, and karyorrhexis in spermatogenic cells. The TUNEL assay was used for the determination of apoptotic cells. Brown-colored apoptotic cells were visualized under the light microscope. TUNEL positive cells were observed in all exposure groups. Triclosan administration was found to cause apoptosis in zebrafish gonads. These findings indicate that triclosan potentially affects fish reproduction, and that its judicious disposal is essential for protecting the environment and maintaining the reproductive potential of fish.

Ultrastructural And Histopathological Investigation Of Seminiferous Tubules Obtained From Azoospermia Cases In The IVF Center

2020 ◽  
pp. 1-3
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Testicular Biopsy ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Testicular Sperm Extraction ◽  
Interstitial Tissue ◽  
Testicular Sperm ◽  
Electron Microscopic ◽  
Important Indicator ◽  
Serum Hormone ◽  
Histopathological Results

Objective: In this study we purposed to explore seminiferous tubules via histopathological and electron microscopic methods in testicular biopsy samples obtained TESE and the relationship between the findings and levels of serum FSH, LH, testosterone hormones. Methods: Azoospermia patients were divided into two groups, a positive testicular sperm extraction (TESE(+)) and a negative testicular sperm extraction (TESE(-)). Testicular tissue from biopsy samples were subjected to the light and electron microscopic tissue preparations. Serum hormone levels of patients were determined and analyzed statistically between the groups. Results: Compared the groups, more remarkable damages were detected in the seminiferous tubulus of no sperm group in the light and electron microscopic examinations. Although inflammation and partly tubule degeneration was observed, spermatogenesis and sperm cells were determined in the tubules of sperm pozitive group. In the light and ultrastructural analysis of negative sperm group, macrophages and mast cells in the interstitial tissue, vacuolization of seminiferous tubules, lipid inclusions and Sertoli cell only syndrome were the significant findings. When analyzed serum FSH, LH and testosterone hormones between the groups, FSH and LH hormones were statistically significant while Testosteron hormone was not significant. Conclusion: As a result in the seminiferous tubules of individuals histopathological results revealed that FSH and LH are important indicator of testicular function but Testosteron has not any effect. It was concluded that high levels of these hormones impair spermatogenesis and cause testicular failure.

OESTRADIOL-17β AND TESTOSTERONE IN RAT TESTIS TISSUE: EFFECT OF GONADO-TROPHINS, LOCALIZATION AND PRODUCTION IN VITRO

1974 ◽  
Vol 60 (3) ◽  
pp. 409-419 ◽  
Author(s):  
F. H. de JONG ◽  
A. H. HEY ◽  
H. J. van der MOLEN
Keyword(s):  
Intravenous Injection ◽  
Testicular Tissue ◽  
Prolonged Treatment ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Tissue Concentrations ◽  
Hypophysectomized Rats ◽  
Trophic Hormone ◽  
Testis Tissue

SUMMARY Concentrations of oestradiol-17β and testosterone were estimated in testicular tissue from intact and hypophysectomized rats. Within 30 min after intravenous injection of human chorionic gonadotrophin (HCG) or follicle-stimulating hormone (FSH) to intact animals the tissue concentrations of both steroids were not significantly changed. Prolonged s.c. administration of HCG (5 days) caused an increase in the tissue levels of both steroids, which was further increased when the prolonged treatment was followed by an intravenous injection with this trophic hormone. FSH had no influence on tissue concentrations of oestradiol-17β or testosterone in hypophysectomized rats. Assay of separated seminiferous tubules and interstitial tissue indicated that oestradiol-17β and testosterone were mainly localized in the interstitial tissue. Incubations of these constituents showed that oestradiol-17β was produced in the seminiferous tubules, while testosterone was produced in the interstitial compartment.

scholarly journals Testicular Morphological Changes Produced by Fluoroquinolones in Adult Male Albino Rats

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Iram Atta
Keyword(s):  
Adult Male ◽  
Leydig Cells ◽  
Morphological Changes ◽  
Sperm Count ◽  
Research Work ◽  
Testicular Tissue ◽  
Reproductive Organs ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats

AbstractObjectives:  The objective of this research work was to observe the testicular morphological changes produced by fluoroquinolones in the reproductive organs of adult male albino rats, and to see whether these changes are reversible after discontinuation of the drugs.Materials and Method:  Eighty adult male albino rats weighing 200 – 300 gms were randomly selected and divided into four groups i.e. A, B, C & D, having 20 animals in each group. A, B & C, were the experimental groups & D served as control group. All the groups were further divided into sub groups 1 & 2. Three fluoroquinolones i.e. Ciprofloxacin (135 mg / kg / day), Ofloxacin (75 mg / kg / day) & Enoxacin (12.5 mg / kg/ day) were given to the groups A, B & C respectively for 42 days. Animals of group D received dis-tilled water only. Animals of sub groups A1, B1, C1 &D1 were sacrificed on 42nd day and testicular tissue was obtained for morphological study. Animals of subgroups A2, B2, C2 & D2 were sacrificed on 84th day and testicular tissue for morphological changes was taken. No of leydig cells, height of epithelium and diameter of seminiferous tubules were taken as experimental parameters for morphological changes.Results:  The study indicated statistically significant (P < 0.05) decrease in height of epithelium, diameter of seminiferous tubules and no. of leydig cells in experimental groups as compared to the control groups.Conclusion:  The changes observed in morphology could lead to decrease in sperm count and testosterone levels. This study suggests gonadotoxic potentials of fluoroquinolones and adds concern to the indiscriminate and widespread use of fluoroquinolones and recommends more rational use of these drugs.

Acute Exposure to the Neonicotinoid Insecticide Imidacloprid of Zebrafish (Danio rerio) Gonads: A Histopathological Approach

2021 ◽  
Vol 57 ◽  
pp. 23
Author(s):  
Cansu Akbulut
Keyword(s):  
Interstitial Fibrosis ◽  
Periodic Acid ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Histological Techniques ◽  
Zona Radiata ◽  
Structural Deterioration ◽  
Neonicotinoid Insecticide

Neonicotinoids are the new class of insecticides that are high target specificity to insects. Imidacloprid is a neonicotinoid that is the most widely used insecticide in the world. As a result of its widespread use in agriculture, imidacloprid interferes with the aquatic system and threatens the aquatic environment. In this study, an investigation of the histopathological effects of imidacloprid on zebrafish gonads was aimed. Zebrafish were exposed to 9.5 mg/L, 19 mg/L, and 38 mg/L of imidacloprid for 5 days, considering the 96 h LC50 value. After dissecting the gonadal tissues, routine histological techniques were applied, and the tissues were stained with Periodic Acid-Schiff (PAS), Toluidine Blue (TB), and Haematoxylin and Eosin (H&E). Sections were examined under a light microscope. While normal gonad histology was observed in the control group, histopathological alternations such as degeneration and union in the seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, and interstitial fibrosis were detected in testicular tissue of the experimental groups. In the ovarian tissues of the experimental groups, structural deterioration in oocytes, autolysis, increase in the number of atretic oocytes, vacuolization in cortical alveoli, thickening and curling in the zona radiata, and opening in the perifollicular layers were detected.

Defective spermatogenesis and testosterone levels in kinase suppressor of Ras1 (KSR1)-deficient mice

2019 ◽  
Vol 31 (8) ◽  
pp. 1369
Author(s):  
Elena Moretti ◽  
Giulia Collodel ◽  
Giuseppe Belmonte ◽  
Daria Noto ◽  
Emanuele Giurisato
Keyword(s):  
Molecular Mechanisms ◽  
Sperm Concentration ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Testosterone Levels ◽  
Transmission Electron ◽  
Human Spermatogenesis ◽  
Testis Tissue

The aim of this study was to clarify the role of the protein kinase suppressor of Ras1 (KSR1) in spermatogenesis. Spermatogenesis in ksr1−/− mice was studied in testicular tissue and epididymal spermatozoa by light and transmission electron microscopy and by immunofluorescence using antibodies to ghrelin and 3β-hydroxysteroid dehydrogenase (3β-HSD). Blood testosterone levels were also assessed. ksr1−/− mice showed reduced epididymal sperm concentration and motility as compared with wild-type (wt) mice. Testis tissue from ksr1−/− mice revealed a prevalent spermatogenetic arrest at the spermatocyte stage; the interstitial tissue was hypertrophic and the cytoplasm of the Leydig cells was full of lipid droplets. Ghrelin signal was present in the seminiferous tubules and, particularly, in the interstitial tissue of wt mice; however, in ksr1−/− mice ghrelin expression was very weak in both the interstitial tissue and tubules. On the contrary, the signal of 3β-HSD was weak in the interstitial tissue of wt and strong in ksr1−/− mice. Testosterone levels were significantly increased in the blood of ksr1−/− mice (P&lt;0.05) as compared with wt. The results obtained reveal the importance of the KSR scaffold proteins in the spermatogenetic process. The study of the molecular mechanisms associated with spermatogenetic defects in a mouse model is essential to understand the factors involved in human spermatogenesis.

Localization and characterization of epidermal growth factor receptors on human testicular tissue by biochemical and immunohistochemical techniques

1990 ◽  
Vol 125 (3) ◽  
pp. 485-NP ◽  
Author(s):  
S. C. Stubbs ◽  
T. B. Hargreave ◽  
F. K. Habib
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Testicular Tissue ◽  
Immunohistochemical Localization ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Testicular Steroidogenesis ◽  
Epidermal Growth ◽  
Immunohistochemical Techniques

ABSTRACT In the present study attempts were made to characterize the epidermal growth factor (EGF) receptor on human testicular tissue. A radioligand exchange assay with 125I-labelled EGF was used to detect a high affinity, low capacity, single binding site in the 105 000 g particulate fraction of human testicular tissue. Binding was optimal at 32 °C following a 40-min incubation with a mean ( ± s.d.) dissociation constant of 327 ±59 pmol/l (d.f. 9). The number of binding sites ranged from 0.07 to 0.21 pmol/mg protein. Competition studies with other peptide hormones including LH, FSH, prolactin, insulin-like growth factor-I, fibroblast growth factor and nerve growth factor have confirmed the specificity of EGF for its receptor. The receptor was also found to be heatlabile and sensitive to trypsinization. Cross-linking experiments using disuccinimidyl suberate revealed major binding species at the 125 kDa region and this is thought to represent a proteolysed form of the receptor. Immunohistochemical localization of the receptors demonstrated their presence in the interstitial tissue and not within the seminiferous tubules. The presence of specific EGF binding in the interstitial tissue suggests that EGF may play some role in testicular steroidogenesis. Journal of Endocrinology (1990) 125, 485–492

AN ASSESSMENT OF THE POSSIBLE ROLE OF 17α,20α-DIHYDROXY-4-PREGNEN-3-ONE IN THE REGULATION OF TESTOSTERONE SYNTHESIS BY RAT AND RABBIT TESTIS

1974 ◽  
Vol 61 (3) ◽  
pp. 401-410 ◽  
Author(s):  
H. W. A. de BRUIJN ◽  
H. J. van der MOLEN
Keyword(s):  
Venous Blood ◽  
Competitive Inhibitor ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Lyase Activity ◽  
Testosterone Biosynthesis

SUMMARY 17α,20α-Dihydroxy-4-pregnen-3-one is a competitive inhibitor of C17,20-lyase activity in rat testicular tissue in vitro and the significance of this inhibition in vitro was evaluated for testosterone biosynthesis in rat and rabbit testis in vivo. It is concluded that 17α,20α-dihydroxy-4-pregnen-3-one is not involved in the regulation of C17,20-activity in vivo, because it was not possible to detect any 17α,20α-dihydroxy-4-pregnen-3-one in rat and rabbit testicular tissue or in testicular venous blood. If present, the levels are lower than 10 pmol/g testis. Levels of 17α-hydroxyprogester-one are in the order of 50 pmol/g testis. The C17,20-lyase has a higher affinity for 17α-hydroxyprogesterone than for 17α,20α-dihydroxy-4-pregnen-3-one and hence inhibition under in-vivo conditions is not favoured. In rat testes the 20α-hydroxysteroid dehydrogenase activity, which can convert 17α-hydroxyprogesterone to 17α,20α-dihydroxy-4-pregnen-3-one, was found to be mainly (97%) localized in the seminiferous tubules and not at the site of testosterone formation in the interstitial tissue.

THE DESTRUCTIVE EFFECT OF CADMIUM ION ON TESTICULAR TISSUE AND ITS PREVENTION BY ZINC

1957 ◽  
Vol 15 (1) ◽  
pp. 56-63 ◽  
Author(s):  
J. PAŘÍZEK
Keyword(s):  
Subcutaneous Administration ◽  
Testicular Tissue ◽  
Endocrine Function ◽  
Male Rats ◽  
Practical Significance ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Destructive Effect ◽  
Zinc Salts ◽  
Rats And Mice

SUMMARY The subcutaneous administration of cadmium salts (cadmium chloride or lactate) to male rats and mice leads to acute destruction of the testes, with destruction of the seminiferous epithelium and interstitial tissue. These changes in turn evoke castration phenomena, but the atrophied accessory sex organs retain the ability to react to testosterone propionate. Within 20 days after the injection of cadmium, proliferation of fibroblasts in the interstitial spaces under the albuginea begins and is accompanied by an extensive formation of new blood vessels. Later, new Leydig cells appear; this is followed by a gradual return of the endocrine function of the testes. The spermatogenic epithelium of the seminiferous tubules, on the other hand, does not regenerate even 133 days after the injection of cadmium. The simultaneous administration of a large dose of zinc salts protects the testes completely against cadmium damage. The mechanism of interaction between these physico-chemically related metals and the theoretical and practical significance of these observations will be studied further.

scholarly journals Developmental Effects of ELF-EMF on Testis and Ovary in Newborn Rats

2020 ◽  
Keyword(s):  
Treatment Group ◽  
Transmission Lines ◽  
Morphological Changes ◽  
Ovary Development ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Interstitial Tissue ◽  
Human Beings ◽  
Pregnant Rats ◽  
Primordial Follicles

Background and Objective: Human beings are unavoidably exposed to ambient electromagnetic fields (EMF) generated from various electrical devices and from power transmission lines. Controversy exists about the effects of EMF on various organs. The aim of this work evaluation the effects of intrauterine exposure to 50Hz electromagnetic field (EMF) on testicular and ovary development. Methods: Pregnant wistar rats were exposed to 3mT, EMF for 21 days, 4 hours/day. Pregnant rats under same condition of treatment group, but off the field as a sham group intended and pregnant rats were used as control in the room. After delivery, testis and ovary were removed from male and female pups, fixed and prepared for light microscopic studies. Results: Microscopic results revealed seminiferous tubules in treatment group in comparison with the control and sham groups were widely separated from each other, in this group in seminiferous tubules vacuolization, detachment of gonocytes from each other, heterochromatic gonocytes and decreasing in interstitial tissue was found. The ovary of the treatment group in comparison to control group showed that oocyte nests were mostly broken and irregularly arranged. The primordial follicles were less developed. Conclusion: In general, as a result of the exposure to EMF during early developmental period, morphological changes in testicular and ovary development were evident, that may well extend till adult stage and may affect fertility.

scholarly journals Effects of Iron Oxide Nanoparticles on Mouse Sperm Parameters and Testicular Tissue

2018 ◽  
Vol 12 (6) ◽  
pp. 39-44
Author(s):  
Sepideh Mirzaei Varzeghani ◽  
◽  
Kazem Parivar ◽  
Mohammad-Amin Abdollahifar ◽  
Amin Karamian ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Testicular Tissue ◽  
Sperm Parameters ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Computer Assisted ◽  
Oxide Nanoparticles ◽  
Interstitial Tissue ◽  
Sperm Analysis

Background: Iron oxide nanoparticles are commonly used for various purposes, such as biomedical, medical, and cosmetic services and research. However, there is a little information about the effects of the nanoparticles on human health. The current investigation was conducted to evaluate the adverse effects of iron oxide nanoparticles (FeNP) on the reproductive organs of mice, such as the testicular tissue and sperm cells. Methods: Twenty-eight male NMRI mice were randomly divided in four groups (N=7). The control group received only a regular diet. The experimental groups were administered FeNP in doses of 50, 150 and 300 mg/Kg intraperitoneally (IP), over four days. Epididymal sperm parameters, such as sperm number and motility were assessed by computer-assisted sperm analysis (CASA). Stereological analysis was also conducted on the histological sections. Results: The results demonstrated that FeNP (300 mg/Kg/day) caused a significant decrease in the sperm parameters, such as motility, spermatogonia, primary spermatocytes, spermatid, Sertoli, Leydig cells, total length of seminiferous tubules, and testicular interstitial tissue volumes. Conclusion: In summary, FeNP affected several reproductive tissue and cellular parameters at the administered dosage. Further research is required to examine the mechanism of action of FeNP the mice reproductive system.

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