Micromorphology of an involucral bract and cypselae of the endemic and relict species Amphoricarpos elegans Albov (Asteraceae, Cardueae) from the Caucasus

In this study, micromorphology of an involucral bract and inner and outer dimorphic cypselae of the relict Amphoricarpos elegans, endemic from western Caucasus, is examined using a scanning electron microscope (SEM) and presented here for the first time. The middle involucral bract is nearly glabrous, with a reticulate-rugulose surface and an oblong-obtusate mucro. Both cypselae exhibit similar features, viz., narrowly oblong-cylindrical or obovate shape with more or less conspicuous longitudinal ribs, an adaxial detachment area, an asymmetrical carpopodium, absence of a pericarp crown and a barbate-aristate pappus made up of narrow, subulate, basally smooth and apically barbate bristles. However, some differences are evident. The outer cypsela is glabrescent and flattened, with an obscure outline of epidermal cells lacking an end wall and with a sunken periclinal surface and striatereticulate structure. Also, the outer cypsela has two entire, narrow, lateral wings, rounded above; and less numerous pappus bristles in one row. In contrast to this, the inner cypsela has a distinct outline of the epidermal cells, which are rod-shaped with a short acute end wall, a swollen periclinal surface and reticulate structure. The pappus of the inner cypsela possesses many more bristles organised in two rows (a biseriate pappus). Also, slightly pentagonal or round nectary remnants are found in the centre of the upper part of the inner cypsela. The taxonomic value of the analysed characters is briefly discussed.

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SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2020-22-12-176-182 ◽

2020 ◽

pp. 176-182

Author(s):

Mamaeva S.N. ◽

Vinokurov R.R. ◽

Munkhalova Ya.A. ◽

Dyakonova D.P. ◽

Platonova V.A. ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Red Blood Cells ◽

Blood Cells ◽

Filtration Rate ◽

Kidney Diseases ◽

First Time ◽

Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

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To the hepatic flora of the Sochi National Park (Western Caucasus)

Novosti sistematiki nizshikh rastenii ◽

10.31111/nsnr/2011.45.301 ◽

2011 ◽

Vol 45 ◽

pp. 301-317 ◽

Cited By ~ 4

Author(s):

N. A. Konstantinova ◽

A. N. Savchenko

Keyword(s):

National Park ◽

Conservation Value ◽

Western Caucasus ◽

The Caucasus ◽

European Species ◽

Red Data Book ◽

Annotated List ◽

New Localities ◽

Data Book ◽

First Time

The annotated list of hepatics of the Sochi National Park includes 80 taxa. It is based on literature records and identification of 250 specimens collected by the authors. Scapania obcordata (Berggr.) S. W. Arnell and Calypogeia fissa (L.) Raddi var. intermedia (C. E. O. Jensen) Jorg. are new for the Caucasus, 17 taxa are new for Krasnodar Territory, 41 hepatics are reported for the first time for the park. New localities of 3 red-listed European species and of 6 species included in the Red Data Book of Krasnodar Territory are cited, indicating significant conservation value of the reserve.

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Capture and scanning electron microscopy of individual snow crystals

Journal of Glaciology ◽

10.3189/s0022143000003981 ◽

1994 ◽

Vol 40 (134) ◽

pp. 195-197

Author(s):

E. W. Wolff ◽

A. P. Reid

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Liquid Nitrogen Temperature ◽

Experimental Methods ◽

Snow Crystal ◽

Snow Crystals ◽

First Time ◽

Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

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Morphology and ecology of Thalassiosira Cleve (Bacillariophyta) species rarely recorded in Brazilian coastal waters

Brazilian Journal of Biology ◽

10.1590/s1519-69842009000500009 ◽

2009 ◽

Vol 69 (4) ◽

pp. 1059-1071 ◽

Cited By ~ 7

Author(s):

M. Garcia ◽

C. Odebrecht

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Water Temperature ◽

Wide Temperature Range ◽

Coastal Waters ◽

Light Microscope ◽

Temperature Range ◽

The World ◽

First Time ◽

Scanning Electron

The detailed description of rarely recorded Thalassiosira species in Brazil is presented with light microscope (LM) and scanning electron microscope (SEM) illustrations. A total of 78 phytoplankton net samples (20 µm) collected between the years 2000 and 2006 in coastal waters of southern Brazilian, Cassino Beach and the estuary of Lagoa dos Patos, were studied in cleaned material using the Axiovert Zeiss LM and Jeol 6060 SEM. Water temperature and salinity of samples and six species are presented: Thalassiosira endoseriata, T. hendeyi, T. lundiana, T. minuscula, T. oceanica and T. wongii. Two species, Thalassiosira hendeyi and T. endoseriata were the most common being observed in all seasons at Cassino Beach in a wide temperature range (10-26 ºC), while only sporadically in the estuary of Lagoa dos Patos. Thalassiosira endoseriata, T. lundiana, T. oceanica and T. wongii are for the first time reported in Brazilian coastal waters. The latter two species, rarely recorded in the world, are fully illustrated based on Brazilian material.

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Taxonomic studies in the genus Haplanthodes (Acanthaceae)

Phytotaxa ◽

10.11646/phytotaxa.516.3.1 ◽

2021 ◽

Vol 516 (3) ◽

Author(s):

PRADIP VIKRAM DESHMUKH ◽

SIDDHARTHAN SURVESWARAN ◽

RAMCHANDRA DNYANOBA GORE ◽

MANOJ MADHWANAND LEKHAK

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Light Microscope ◽

Pollen Grains ◽

Prolate Spheroidal ◽

New Variety ◽

Exine Ornamentation ◽

Taxonomic Studies ◽

First Time ◽

Scanning Electron

The endemic Indian genus Haplanthodes (Acanthaceae) is revised. Four species, viz. H. neilgherryensis, H. plumosa, H. tentaculata and H. verticillata and a new variety, H. neilgherryensis var. toranganensis are recognized. Lectotype is designated for Haplanthodes, Haplanthus plumosus and H. verticillaris. The nomenclature of the Linnaean name Ruellia tentaculata is also discussed. Micromorphology of seed using light microscope (LM) and scanning electron microscope (SEM) is observed for the first time, and two types, reticulate and micro papillate, have been recognized based on surface sculpturing pattern. The genus differs from the related genera Andrographis and Haplanthus by distinctly two grooved seeds with hygroscopic hairs. Pollen grains of the genus are oblate or prolate spheroidal, distinctly triangular, trizonocolporate with reticulate exine ornamentation.

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Description of two new species, Aphanolaimus strilliae n. sp. (Nematoda:Aphanolaimidae) and Makatinus africanus n. sp. (Nematoda: Aporcelaimidae), and SEM observations on three known species from freshwater sources in the Telperion Nature Reserve (Mpumalanga, South Africa)

Zootaxa ◽

10.11646/zootaxa.4651.2.1 ◽

2019 ◽

Vol 4651 (2) ◽

pp. 201-234

Author(s):

CHANTELLE GIRGAN ◽

MARIETTE MARAIS ◽

HENDRIKA FOURIE ◽

LOURENS TIEDT ◽

ANTOINETTE SWART

Keyword(s):

South Africa ◽

Electron Microscope ◽

New Species ◽

Nature Reserve ◽

Long Tail ◽

Lateral Field ◽

Lateral Body ◽

Two New Species ◽

First Time ◽

Scanning Electron

Aphanolaimus strilliae n. sp. and Makatinus africanus n. sp. are described from freshwater sources in the Telperion Nature Reserve, Mpumalanga, South Africa. Aphanolaimus strilliae n. sp. is characterised by a body length of 1240–1613 μm, more than 800 body annules, lateral field originating between the first and second lateral body pore at the 34th–46th annule, first lateral body pore located at the 25th–35th annule, vagina V-shaped and bent anteriorly, 142–195 µm long uterus and 165–207 µm long tail with spinneret offset. This species is ovoviviparous and no males were found. Makatinus africanus n. sp. is characterised by a large, thick body (3228–4128 μm long, a = 30–39 wide), slightly set off lip region with amalgamated lips; 31–34 μm long odontostyle; long tongue-shaped cardia, and stout, short tail (30–43 μm long) with a small peg / digitate extension and male absent. Populations of three known species, Chronogaster africana, Eutobrilus annetteae and Neotobrilus ampiei, from fresh water at the Telperion Nature Reserve are described and scanning electron microscope graphs of these species published for the first time.

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Description of adults and larvae of Orientelmis parvula (Nomura & Baba, 1961) (Coleoptera: Elmidae) with their molecular phylogenetic analysis

Zootaxa ◽

10.11646/zootaxa.4568.3.4 ◽

2019 ◽

Vol 4568 (3) ◽

pp. 483 ◽

Cited By ~ 3

Author(s):

MASAKAZU HAYASHI ◽

HIROYUKI YOSHITOMI ◽

YUUKI KAMITE ◽

TAKUYA KOBAYASHI ◽

TEIJI SOTA

Keyword(s):

Phylogenetic Analysis ◽

Electron Microscope ◽

Systematic Position ◽

Larval Morphology ◽

Mitochondrial Coi ◽

Rare And Endangered Species ◽

Molecular Phylogenetic ◽

Rare And Endangered ◽

First Time ◽

Scanning Electron

The elmid beetle Orientelmis parvula (Nomura & Baba, 1961) is a rare and endangered species in Japan. Mouth parts and genitalia in adults of both sexes and larval morphology are described based on scanning electron microscope observations. The larva of the genus Orientelmis Shepard, 1998 is described for the first time. The systematic position of the genus is discussed based on the morphology and a phylogenetic tree inferred from the mitochondrial COI, and nuclear ArgK and 18S gene sequences. A new key for larvae of known species and genera of Japanese Elmidae is given.

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Choosing a Scanning Electron Microscope

Microscopy and Microanalysis ◽

10.1017/s1431927600024600 ◽

1998 ◽

Vol 4 (S2) ◽

pp. 896-897

Author(s):

W. A. Lambe ◽

P.M. Brady

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Selection Process ◽

The Other ◽

Hard Data ◽

Methodical Approach ◽

Electron Microscopes ◽

Scanning Electron Microscopes ◽

First Time ◽

Scanning Electron

The variety of instrumentation available to the researcher today can be overwhelming and confusing. Scanning Electron Microscopes (“SEM's) are no exception, and choosing one can often serve as an exercise in dealing with complexity. First time purchasers are most at risk, being subject to a barrage of information that attempts to sway the purchaser in one direction or the other. As a result, one can sometimes be drawn to the details of the latest “high end” performance parameter, while overlooking the basics. At its worst, the selection process can degrade to one of vague guesswork with little hard data to serve as a compass.By applying a methodical approach to define your individual requirements, carefully designed tests of actual instruments, and discussions with your collaborators, potential and experienced users, one can begin to ensure a successful selection process.

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Spore morphology of Selaginella (Selaginellaceae) from China and its systematic significance

Phytotaxa ◽

10.11646/phytotaxa.237.1.1 ◽

2015 ◽

Vol 237 (1) ◽

pp. 1 ◽

Cited By ~ 6

Author(s):

Xin-Mao Zhou ◽

Li-Jü Jiang ◽

Liang Zhang ◽

Xin-Fen Gao ◽

Zhao-Rong He ◽

...

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Light Microscope ◽

Morphological Characteristics ◽

Spore Morphology ◽

Systematic Significance ◽

First Time ◽

Scanning Electron

Using light microscope (LM) and scanning electron microscope (SEM), the megaspores and microspores of 77 samples representing ca. 70 species of Selaginella from China are observed. Combing previous studies, the spore morphology of nearly all documented Selaginella species from China were reviewed. Based on the morphological characteristics in megaspores and/or microspores, we divided the spores of Chinese species into 15 types and three types are further divided into various subtypes. Each type and subtype are described in detail and a key to the types and subtypes of spores is given. For the first time, the systematic significance of microspores of Selaginella are discussed, and the results indicate that microspores of Selaginella are significant in the systematics of Selaginella. Some important morphological characteristics in spores (e.g., color, micro-sculpture, size, etc.), often been neglected in previous studies, are introduced. Some spore-morphological synapomorphies of the clades and subclades, identified by recent molecular work (Zhou et al. 2015a), are well established. Using the spore morphology, the delimitation of some taxonomically difficult species in Selaginella is assessed.

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Scanning electron microscopy of the cuticular armature of the nematode Gnathostoma spinigerum Owen, 1836 from cats in Laos

Journal of Helminthology ◽

10.1017/s0022149x00012244 ◽

1990 ◽

Vol 64 (3) ◽

pp. 255-262 ◽

Cited By ~ 3

Author(s):

Tomáš Scholz ◽

Oleg Ditrich

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Electron Microscope ◽

Spatial Arrangement ◽

Felis Catus ◽

The Body ◽

Immature Female ◽

Gnathostoma Spinigerum ◽

First Time ◽

Scanning Electron

ABSTRACTThe body surface of an immature female Gnathostoma spinigerum found for the first time in the definitive host (Felis catus f. domestica) in Laos was studied using a scanning electron microscope. All types of cuticular spines, which are one of the most important features for species identification of gnathostomid nematodes, together with their spatial arrangement, are described and figured.

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