Antifungal and synergistic activity of five plant essential oils from Serbia against Trichoderma aggressivum f. europaeum Samuels & W. Gams

Five essential oils isolated from plants originating from Serbia and ten combinations of the selected essential oils were assayed to test their inhibitory and fungicidal activity against Trchoderma aggressivum f. europaeum Samuels & W. Gams using two distinctive methods: microdilution and fumigant macrodilution methods. The strongest activity was demonstrated by spearmint (Mentha spicata L.) and thyme (Thymus serpyllum L.) oils at the minimum inhibitory concentration (MIC) of 6.25 ?l ml-1 using microdilution, and 0.16 ?l ml-1 of air using fumigant macrodilution method. The antifungal activity of basil (Ocimum basilicum L.) and peppermint (Mentha piperita L.) was medium, while the oil extracted from St. John?s wort (Hypericum perforatum L.) exhibited the lowest activity. None of the selected essential oils exhibited fungicidal effect at minimal fungicidal concentrations (?FC) of over 25 ?l ml-1 or 0.32 ?l ml-1of air, using micro- and macrodilution, respectively. When microdilution was used, the strongest antifungal activity was demonstrated by two oil combinations: spearmint-thyme and spearmint-peppermint, having MIC and MFC values of 3.75 ?l ml-1. The lowest activity was demonstrated by the basil-St. John?s wort essential oil combination, at 30 ?l ml-1 MIC, and MFC exceeding 30 ?l ml-1. The obtained results indicate possible synergistic effects of essential oils and their components.

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Antifungal activity of six plant essential oils from Serbia against Trichoderma aggressivum f. europaeum

Pesticidi i fitomedicina ◽

10.2298/pif1404291d ◽

2014 ◽

Vol 29 (4) ◽

pp. 291-297 ◽

Cited By ~ 2

Author(s):

Rada Djurovic-Pejcev ◽

Ivana Potocnik ◽

Svetlana Milijasevic-Marcic ◽

Biljana Todorovic ◽

Emil Rekanovic ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Juglans Regia ◽

Mentha Piperita ◽

Button Mushroom ◽

Minimum Fungicidal Concentration ◽

Plant Essential Oils ◽

Hypericum Perforatum L ◽

Green Mould ◽

Main Components

Six essential oils (EOs) extracted from plants originating in Serbia were assayed for inhibitory and fungicidal activity against a major fungal pathogen of button mushroom causing green mould disease, Trichoderma agressivum f. europaeum. The strongest activity was demonstrated by the oils of basil (Ocimum basilicum L.) and peppermint (Mentha piperita L.). Medium antifungal activity of St. John's wort (Hypericum perforatum L.) and walnut [Juglans regia (F)] oils was also recorded. Oils extracted from yarrow (Achillea millepholium L.) and juniper (Juniperus communis L.) exhibited the lowest activity. Peppermint oil showed fungicidal effect on the pathogen, having a minimum fungicidal concentration of 0.64 ?l ml-1. The main components of peppermint essential oil were menthone (37.02%), menthol (29.57%) and isomenthone (9.06%).

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THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2018.6.17 ◽

2018 ◽

Vol 17 (6) ◽

pp. 167-174 ◽

Cited By ~ 1

Author(s):

Małgorzata Schollenberger ◽

Tomasz M. Staniek ◽

Elżbieta Paduch-Cichal ◽

Beata Dasiewicz ◽

Agnieszka Gadomska-Gajadhur ◽

...

Keyword(s):

Essential Oils ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Mentha Piperita ◽

Mentha Spicata ◽

Plant Essential Oils ◽

Plant Pathogenic Bacteria ◽

Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

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Acaricidal and Insect Antifeedant Effects of Essential Oils From Selected Aromatic Plants and Their Main Components

Frontiers in Agronomy ◽

10.3389/fagro.2021.662802 ◽

2021 ◽

Vol 3 ◽

Author(s):

Félix Valcárcel ◽

A. Sonia Olmeda ◽

Marta G. González ◽

Maria Fe Andrés ◽

Juliana Navarro-Rocha ◽

...

Keyword(s):

Essential Oils ◽

Synergistic Effects ◽

Mentha Piperita ◽

Thymus Vulgaris ◽

Aromatic Plants ◽

Mentha Spicata ◽

Botanical Pesticides ◽

Main Components ◽

Further Development ◽

Satureja Montana

This work has demonstrated the ixodicidal and insect antifeedant effects of essential oils from 14 experimentally cultivated aromatic plants. The strong ixodicidal and antifeedant oils corresponded to Thymus zygis, Thymus vulgaris, Satureja montana, Oreganum virens, and Mentha suaveolens. The moderately active oils were from Lavandula angustifolia, Mentha piperita, Mentha spicata, Artemisa herba-alba, and Rosmarinus officinalis. The most effective larvicidal and antifeedant compounds were piperitenone oxide, carvacrol, piperitenone, and thymol, explaining the effects of the most active essential oils. The rest of the tested compounds were not ixodicidal or antifeedant. Therefore, the activity of moderately active oils cannot be explained by their main components (linalyl acetate, linalool, menthone, menthol, limonene, camphor, 1,8-cineole, p-cymene, α-pìnene, and carvone), suggesting synergistic effects. Considering the ixodicidal and antifeedant effects of these extracts, the plants have been ranked in relation to Thymus vulgare, a commercial biopesticide ingredient, for their potential as botanical pesticides. T. zygis, S. montana, and M. suaveolens ranked over T. vulgaris as ixodicidal agents and S. montana as insecticidal. Therefore, we propose the plant populations of S. montana, T. zygis, and M. suaveolens tested here for further development as biopesticide ingredients.

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Efficacy of Plant Essential Oils on Postharvest Control of Rots Caused by Fungi on Different Stone Fruits In Vivo

Journal of Food Protection ◽

10.4315/0362-028x.jfp-12-342 ◽

2013 ◽

Vol 76 (4) ◽

pp. 631-639 ◽

Cited By ~ 47

Author(s):

JORGE GIOVANNY LOPEZ-REYES ◽

DAVIDE SPADARO ◽

AMBRA PRELLE ◽

ANGELO GARIBALDI ◽

MARIA LODOVICA GULLINO

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Stone Fruit ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Mentha Piperita ◽

Thymus Vulgaris ◽

Synergistic Activity ◽

Plant Essential Oils

The antifungal activity of plant essential oils was evaluated as postharvest treatment on stone fruit against brown rot and grey mold rot of stone fruit caused by Monilinia laxa and Botrytis cinerea, respectively. The essential oils from basil (Ocimum basilicum), fennel (Foeniculum sativum), lavender (Lavandula officinalis), marjoram (Origanum majorana), oregano (Origanum vulgare), peppermint (Mentha piperita), rosemary (Rosmarinus officinalis), sage (Salvia officinalis), savory (Satureja montana), thyme (Thymus vulgaris), and wild mint (Mentha arvensis) were tested at two different concentrations on apricots (cv. Kyoto and cv. Tonda di Costigliole), nectarines (cv. Big Top and cv. Nectaross) and plums (cv. Italia and cv. TC Sun). The volatile composition of the essential oils tested was determined by gas chromatography–mass spectrometry analysis. The treatments containing essential oils from oregano, savory, and thyme at 1% (vol/vol) controlled both B. cinerea and M. laxa growing on apricots cv. Tonda di Costigliole and plums cv. Italia and cv. TC Sun; however, the same treatments were phytotoxic for the carposphere of nectarines cv. Big Top and cv. Nectaross. Treatments with 10% (vol/vol) essential oils were highly phytotoxic, notwithstanding their efficacy against the pathogens tested. The essential oils containing as major components α-pinene, p-cymene, carvacrol, and thymol showed similar results on stone fruit, so their antimicrobial activity and the phytotoxicity produced could be based on the concentration of their principal compounds and their synergistic activity. The efficacy of the essential oil treatments on control of fungal pathogens in postharvest depended on the fruit cultivar, the composition and concentration of the essential oil applied, and the length of storage.

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Chemical Composition and Antimicrobial Properties of Mentha × piperita cv. ‘Kristinka’ Essential Oil

Plants ◽

10.3390/plants10081567 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1567

Author(s):

Ippolito Camele ◽

Daniela Gruľová ◽

Hazem S. Elshafie

Keyword(s):

Antifungal Activity ◽

Xanthomonas Campestris ◽

Fruits And Vegetables ◽

Antimicrobial Properties ◽

Pathogenic Fungi ◽

Cell Membrane Permeability ◽

Penicillium Expansum ◽

Mentha Piperita ◽

Plant Essential Oils ◽

Main Components

Several economically important crops, fruits and vegetables are susceptible to infection by pathogenic fungi and/or bacteria postharvest or in field. Recently, plant essential oils (EOs) extracted from different medicinal and officinal plants have had promising antimicrobial effects against phytopathogens. In the present study, the potential microbicide activity of Mentha × piperita cv. ‘Kristinka’ (peppermint) EO and its main constituents have been evaluated against some common phytopathogens. In addition, the cell membrane permeability of the tested fungi and the minimum fungicidal concentrations were measured. The antifungal activity was tested against the following postharvest fungi: Botrytis cinerea, Monilinia fructicola, Penicillium expansum and Aspergillus niger, whereas antibacterial activity was evaluated against Clavibacter michiganensis, Xanthomonas campestris, Pseudomonas savastanoi and P. syringae pv. phaseolicola. The chemical analysis has been carried out using GC-MS and the main components were identified as menthol (70.08%) and menthone (14.49%) followed by limonene (4.32%), menthyl acetate (3.76%) and β-caryophyllene (2.96%). The results show that the tested EO has promising antifungal activity against all tested fungi, whereas they demonstrated only a moderate antibacterial effect against some of the tested bacteria.

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Chemical constituents, in vitro antibacterial and antifungal activity of Mentha×Piperita L. (peppermint) essential oils

Journal of King Saud University - Science ◽

10.1016/j.jksus.2017.07.013 ◽

2019 ◽

Vol 31 (4) ◽

pp. 528-533 ◽

Cited By ~ 16

Author(s):

Nagarjuna Reddy Desam ◽

Abdul Jabbar Al-Rajab ◽

Mukul Sharma ◽

Mary Moses Mylabathula ◽

Ramachandra Reddy Gowkanapalli ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Chemical Constituents ◽

Mentha Piperita ◽

Antibacterial And Antifungal Activity ◽

Antibacterial And Antifungal

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Antifungal activity of Mentha piperita and Carum carvi essential oils

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1733201p ◽

2017 ◽

pp. 201-207 ◽

Cited By ~ 1

Author(s):

Dragana Plavsic ◽

Gordana Dimic ◽

Djordje Psodorov ◽

Dragan Psodorov ◽

Ljubisa Saric ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Biologically Active ◽

Mentha Piperita ◽

Diffusion Method ◽

Aromatic Plants ◽

Disc Diffusion Method ◽

Penicillium Aurantiogriseum

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.

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Antifungal activity of selected volatile essential oils against Penicillium sp.

Open Life Sciences ◽

10.1515/biol-2020-0045 ◽

2020 ◽

Vol 15 (1) ◽

pp. 511-521 ◽

Cited By ~ 1

Author(s):

Soňa Felšöciová ◽

Nenad Vukovic ◽

Paweł Jeżowski ◽

Miroslava Kačániová

Keyword(s):

Antifungal Activity ◽

Pinus Sylvestris ◽

Essential Oils ◽

Mentha Piperita ◽

Pinus Mugo ◽

Origanum Vulgare ◽

Foeniculum Vulgare ◽

Satureja Hortensis ◽

Pimpinella Anisum ◽

Carum Carvi

AbstractPhytopathogenic fungi have been responsible for considerable economic losses in vineyards, and therefore, more attention should be paid to the development and implementation of preventative treatment that is environmentally friendly. The aim of this study was to evaluate the antifungal activity of ten essential oils (EOs) (viz. Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.). For the antifungal activity evaluation against Penicillium brevicompactum, P. citrinum, P. crustosum, P. expansum, P. funiculosum, P. glabrum, P. chrysogenum, P. oxalicum, P. polonicum and Talaromyces purpurogenus a disc diffusion method was used. The ten EOs exhibited different antifungal properties. Three tested EOs (Carum carvi L., Satureja hortensis L. and Pimpinella anisum L.) at concentrations of 0.75, 0.50, 0.25 and 0.125 µL/mL showed antifungal activity, inhibiting the mycelial growth. The Origanum vulgare L. EOs exhibited a lower level of inhibition. Overall, Lavandula angustifolia Mill., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Pimpinella anisum L. and Rosmarinus officinalis L. were effective as fungicidal agents but their efficiency varied between the strains of fungi. Carum carvi L. showed strong antifungal activity against all tested strains at both full strength and reduced concentrations. These EOs could be considered as potential sources of antifungal compounds for treating plant fungal diseases.

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ANTIBACTERIAL AND ANTI-YEAST EFFECTS OF SOME ESSENTIAL OILS

Proceedings of the Mongolian Academy of Sciences ◽

10.5564/pmas.v58i2.1006 ◽

2018 ◽

pp. 68-76

Author(s):

Urjinlham R ◽

Oyunbileg B ◽

Anumandal O ◽

Sainbileg P ◽

Rentsenkhand Ts

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Mentha Piperita ◽

Lag Phase ◽

Gram Negative Bacteria ◽

Synergistic Activity ◽

Aromatic Plants ◽

Gram Positive ◽

Gram Negative ◽

Ledum Palustre

Essential oils from aromatic plants of Ledum palustre L. and Mentha piperita L. were extracted using steam distillation and the antimicrobial effects were evaluated alone and in combinations against food-borne pathogens of Gram-positive and Gram-negative bacteria and food related-yeasts (Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae, Schizosaccharomyce spombe, and Geotrichum candidum). Essential oils from two aromatic plants used in this study showed the antimicrobial activity against bacteria and yeast, which was found to be concentration dependent. The effects of Ledum palustre L. and Mentha piperita L. plants essential oils against yeast and Gram-negative bacteria at concentrations of 0.5 ul/ml and 0.25 ul/ml extended lag phase or adaptation phase by 48 hours, respectively. Gram-positive bacteria were found to be susceptible to the studied two plants essential oils. When the effects of antimicrobial activity of two essential oils were tested in combination against bacteria and yeast in vitro, the MIC value was in the range of 0.125-0.5 ul/ml and showed synergistic activity against E.coli and additive values against B.subtilis and S.cerevisiae.

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