scholarly journals Metabolic profile of sows at different parities and with different litter size

2014 ◽  
Vol 68 (1-2) ◽  
pp. 77-88
Author(s):  
Horea Samanc ◽  
Zeljko Sladojevic ◽  
Ivan Vujanac ◽  
Radisa Prodanovic
Keyword(s):  
Blood Serum ◽  
Litter Size ◽  
Metabolic Profile ◽  
Total Bilirubin ◽  
Inorganic Phosphorus ◽  
Albumin Concentration ◽  
Lactation Period ◽  
Serum Samples ◽  
Significant Difference ◽  
Total Bilirubin Concentration

The aim of this work was to investigate the change of metabolic profile of sows during the lactation period, taking into consideration both parity and number of piglets in a litter (litter size). In order to do this, 30 sows immediately after farrowing were taken and divided into three groups: sows with two farrowing (first group; n=10), ones with four farrowing (second group; n=10), and ones with five and more farrowing (third group; n=10). In regard to a litter size, 20 sows were divided into two groups: sows with eight piglets (first group; n=10), and sows with 10 and more piglets (second group; n=10). All the sows included in the experiment were mestizos of large Yorkshire and Swedish Landrace. Blood samples were taken on the 2nd and 28th day of lactation. In the blood serum samples there were determined the concentration of total proteins, albumins, Total bilirubin, calcium and inorganic phosphorus. The results have shown that there was not a significant difference in proteinemia between the groups on the 2nd day after farrowing, while on the 28th day of lactation, proteinemia was significantly lower only in the group with ten or more piglets in a litter. In all the groups, except for the group with ten or more piglets in the litter, proteinemia was significantly higher on the 28th compared to the 2nd day of lactation. Albumin concentration in blood serum of different groups of sows followed the same trend as protein concentration, with the only difference that albuminemia in sows with ten or more piglets in a litter was approximately the same on the 2nd and 28th day of lactation. Total bilirubin concentration in blood serum on the 2nd day after farrowing was equal in different groups of sows, provided that in sows with five or more farrowings it was significantly higher in regard to other groups. Similar divergence was also determined on the 28th day after farrowing. The sows of 4th parity had significantly lower and the ones with 10 or more farrowings higher bilirubinemia on the 28th compared to the 2nd day of lactation. Glycaemia was significantly lower in the sows with ten or more piglets in a litter in regard to other groups both on the 2nd and 28th day of lactation. Calcaemia was significantly lower on the 2nd in regard to the 28th day of lactation in all the groups, except for the group of sows with ten or more piglets in a litter. In the group with 10 or more piglets in a litter, calcaemia was significantly lower compared to other groups both on the 2nd and 28th day. The sows of 4. Parity had significantly lower phosphataemia in regard to other groups both on the 2nd and 28th day of lactation. Moreover, on the 28th day of lactation, the sows with 10 or more piglets in a litter had significantly lower phosphataemia in regard to all the other groups, except for the group of sows of 4th parity. Phosphataemia varied between the 2nd and 28th day of lactation in neither group except for the group of sows with 10 or more piglets in a litter. On the basis of the obtained results it can be concluded that between the 2nd and 28th day of lactation significant changes in concentrations of some parameters of metabolic profile in sows in lactation occur. The determined changes are most pronounced in elderly sows as well as in the ones with ten or more piglets in a litter, being a result of impeded bile flow or metabolic load due to the increased activity of mammary glands.

scholarly journals Variability of blood serum biochemical parameters in Karakachan sheep

2015 ◽  
Vol 31 (1) ◽  
pp. 55-62 ◽  
Author(s):  
O. Stevanovic ◽  
M. Stojiljkovic ◽  
D. Nedic ◽  
D. Radoja ◽  
V. Nikolic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Age Groups ◽  
Balkan Peninsula ◽  
Inorganic Phosphorus ◽  
Sheep Breed ◽  
Mean Values ◽  
Indigenous Sheep ◽  
Significant Difference ◽  
Glutamyl Transferase

Karakachan sheep represents an endangered, indigenous sheep breed from Balkan Peninsula. There is relatively little information about the characteristics of this sheep breed in the veterinary scientific literature. The aim of this research was an examination of certain metabolic profile parameters of the Karakachan sheep blood serum, and variability of their concentrations in comparison to age and some other indigenous sheep breeds from Balkans. Examination was conducted on 14 clinically healthy sheep divided in two age groups. Blood samples were collected by puncture of v. jugularis and blood serum was separated after spontaneous coagulation. The concentrations of total protein, albumin, calcium, inorganic phosphorus, aspartate amino transferase (AST) and ?- glutamyl transferase (GGT) were determined. In relation to age of Karakachan sheep, statistically significant difference between the calculated mean values of examined parameters was not observed. A statistically significant difference was found between the mean concentrations of the studied parameters in Karakachan sheep and other breeds in total protein (Tsigai, Dubrovnik and Dalmatian sheep), albumin (Dalmatian), calcium and inorganic phosphorus (Tsigai, Dubrovnik) and AST's (Dalmatian, Karakachan sheep from Bulgaria).

scholarly journals Comparison Between Measured and Calculated Free Calcium Values at Different Serum Albumin Concentrations

2016 ◽  
Vol 8 (02) ◽  
pp. 071-076 ◽  
Author(s):  
Altaf Ahmad Mir ◽  
Bela Goyal ◽  
Sudip Kumar Datta ◽  
Saidaiah Ikkurthi ◽  
Arnab Pal
Keyword(s):  
Serum Albumin ◽  
Tertiary Care ◽  
Free Calcium ◽  
Albumin Concentration ◽  
Care Hospital ◽  
Serum Samples ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Clinical Biochemistry Laboratory ◽  
The One

ABSTRACT Introduction: Free ionic calcium is the metabolically active component of total calcium (TCa) in blood. However, most laboratories report TCa levels that are dependent on serum albumin concentration. Hence, several formulae have evolved to calculate free calcium levels from TCa after adjustment for albumin. However, free calcium can directly be measured using direct ion selective electrodes rather than spectrophotometric methods used in autoanalyzers. Objectives: This study compares the levels of free calcium obtained by measurement by direct ion selective electrode (ISE) and the one calculated as a function of TCa by formulae. Materials and Methods: A total of 254 serum samples submitted to clinical biochemistry laboratory of a tertiary care hospital were analyzed for total protein, albumin, and TCa by standard spectrophotometric methods and for free calcium by direct ISE. Three commonly used formulae viz. Orrell, Berry et al. and Payne et al. were used to calculate adjusted TCa. Calculated free calcium was obtained by taking 50% of these values. Results: A significant difference (P < 0.05) was observed between calculated free calcium by all the three formulae and measured free calcium estimated by direct ISE using paired t-test and Bland–Altman plots. Conclusion: Formulae for predicting free calcium by estimating TCa and albumin lacks consistency in prediction and free calcium should be evaluated by direct measurement.

scholarly journals Haematological and biochemical parameters in the blood of an indigenous Croatian breed – Istrian cattle

2014 ◽  
Vol 57 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Martina Bedenicki ◽  
Dalibor Potocnjak ◽  
Ivica Harapin ◽  
Berislav Radisic ◽  
Marko Samardzija ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Metabolic Profile ◽  
Total Bilirubin ◽  
Adult Population ◽  
Inorganic Phosphorus ◽  
Physiological Status ◽  
Differential Analysis ◽  
Physiological Range

Abstract. Haematological and biochemical parameters were examined in the blood of 87 cows, bulls and oxen of the indigenous Croatian breed, Istrian cattle. The sample represents 11.93 % of the total adult population. This breed is classified as highly endangered. The age of tested animals was between 2 and 17 years. Cattle were divided into three groups, animals aged 2 to 6 years (n=19), 7 to 10 years (n=37), and older than 10 years (n=31). Animals were kept in the area of Istria County, mostly in stables though occasionally at pasture. The cows were milked twice daily and gave an average of 1 000 litres of milk per year. Erythrocytes, leukocytes, haemoglobin, haematocrit and mean corpuscular volume were determined. Differential analysis was performed by counting blood cells in the blood smear after Pappenheim staining. Biochemical parameters such as total cholesterol, triglycerides, urea, creatinine, total bilirubin, calcium and inorganic phosphorus were determined. The activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase was determined by spectrophotometry. These biochemical and haematological parameters of this Istrian breed were within the physiological range, while slight deviations were observed for certain parameters. The greatest deviation from the physiological range was shown in the concentrations of urea and creatinine. These results may contribute to a better understanding of the metabolic profile and haematological indicators for estimating the physiological status of these endangered cattle in Istria, for further investigation and for diagnostic purposes.

DYNAMICS OF AN INTERIOR INDICATORS OF HOLSTEIN BREED HEIFERS AND THEIR MOTHERS IN THE TRANSITION LACTATION PERIOD

1970 ◽  
pp. 57-62
Author(s):  
G. G. Karlikova ◽  
R. А. Rykov
Keyword(s):  
Blood Serum ◽  
Total Bilirubin ◽  
High Energy ◽  
Blood Protein ◽  
Lactation Period ◽  
Normal Range ◽  
Total Blood ◽  
Upper Limit ◽  
Group 3 ◽  
Group 1

The article deals with a comparative analysis of biochemical and morphological parameters of blood of Holstein cows during the transit period of lactation and obtained from them heifers of the dairy period. The content of globulin in the blood serum of group 1 cows at 1 month of lactation is 56,0 g/l, at 3 months 53,9 g / l, which is significantly higher than the level in other groups (P≤0,05). In group 3 cows at the 3rd month of lactation, creatinine significantly increased to 74,8 mmol/l (P≤0,05). The blood content of alkaline phosphatase in 1 month of lactation in all examined animals is normal. The blood glucose level in cows is kept in the range of 3,6-4,0 at 1 month and 2,7 – 4,3 at 3 months of lactation (at the norm of 2,0 – 4,8 mmol/l). The concentration of total blood protein in calves at 3 months is within the normal range, but the variation was from 70,3 in group 1 to 84,4 g / l in group 3 (P≤0,05). Significant changes in the serum creatinine content at the 3rd month of life of experimental calves of group 3 up to 73,6 mmol/l (P≤0,10) within the normal range determine the intensity of adaptive mechanisms. Glucose in the blood serum of heifers is at the upper limit of the norm in groups 1 and 2 – 4,9 mmol/l (the norm is 4,8 mmol/l), which indicates a fairly high energy security of the calves ' diet. Total bilirubin in the blood serum of heifers of groups 1 and 2 is slightly higher than normal (the upper limit of the norm is 8,15) – 9,3 and 8,7 mmol/l. By the third month, the total bilirubin content in the blood of group 2 heifers increased to 10,7 mmol/l (P≤0,05), in the rest – within the normal range.

Mechanized determination of the apparent unbound unconjugated bilirubin concentration in serum.

1979 ◽  
Vol 25 (8) ◽  
pp. 1444-1447 ◽  
Author(s):  
R P Wennberg ◽  
L F Rasmussen ◽  
C E Ahlfors ◽  
T Valaes
Keyword(s):  
Total Bilirubin ◽  
Gel Filtration ◽  
Operation Time ◽  
Serum Samples ◽  
Bilirubin Concentration ◽  
Coefficients Of Variation ◽  
Control Serum ◽  
Total Bilirubin Concentration ◽  
Unbound Bilirubin

Abstract The peroxidase method for determining the apparent unbound bilirubin concentration in serum has been automated by use of a programmable, computer-directed spectrophotometer. This mechanized assay determines the total bilirubin concentration and apparent unbound bilirubin concentration in serum samples and titrates the serum with bilirubin to estimate the effect of increasing total bilirubin concentrations on the apparent unbound bilirubin concentration. The entire analysis requires 0.1 mL of serum and 4 min operation time, as compared with about 30 min for the manual method. The coefficients of variation for determination of the apparent unbound bilirubin concentration in bilirubin-enriched commercial control serum were 2.8% within-day and 5.6% between-day. Bilirubin--albumin binding in serum samples from infants with severe hyperbilirubinemia was analyzed by the manual peroxidase method, the automated peroxidase method, and Sephadex gel filtration. Good correlation was found among all three methods.

scholarly journals Determination of blood serum calcium, inorganic phoshorus and magnesium in different productive stages of holstein dairy cows

2019 ◽  
Vol 73 (1) ◽  
pp. 10-16
Author(s):  
Radojica Djokovic ◽  
Marko Cincovic ◽  
Vladimir Kurcubic ◽  
Zoran Ilic ◽  
Milun Petrovic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Calcium ◽  
Serum Magnesium ◽  
Late Pregnancy ◽  
Inorganic Phosphorus ◽  
Lactation Period ◽  
Early Lactation ◽  
Macro Elements

Introduction. The aim of this study was to determine and compare levels of blood serum calcium, inorganic phosphorus and magnesium in the peripartal period and during mid lactation in Holstein dairy cattle. Materials and Methods. Blood samples were collected from 12 cows in late pregnancy, 12 early lactation cows, and 12 mid lactation cows. Results and Conclusions. Serum calcium and inorganic phosphorus in blood of dairy cows in early and mid lactation were significantly lower compared to the values in the blood serum of dairy cows in late pregnancy, probably indicating the increased use of these macro elements by the mammary gland at the early stages of lactation. When the cows were in the lactation period, blood serum magnesium levels were significantly lower compared to the values of dairy cows during late pregnancy, probably indicating the increased use of magnesium by the dairy cows during lactation. The results show that the homeostasis of the macro elements examined in the blood of transition and mid lactation dairy cows was maintained, suggesting their adequate supply from alimentary sources.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2020 ◽  
Vol 13 (9) ◽  
pp. 1854-1857
Author(s):  
Aida Abultdinova ◽  
Isatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances. Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β. Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

Metabolomic Model For Chronic Gvhd Diagnosis

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 3304-3304
Author(s):  
Marcos Rodrigo Alborghetti ◽  
Maria Elvira P Corrêa ◽  
Andreia Aparecida da Silva ◽  
Eliana C M Miranda ◽  
Carmino A De Souza ◽  
...  
Keyword(s):  
Blood Serum ◽  
Hierarchical Clustering ◽  
Research Funding ◽  
Metabolic Profile ◽  
National Laboratory ◽  
Chronic Gvhd ◽  
Sample Collection ◽  
Serum Samples ◽  
Hematopoietic Stem ◽  
Potential Biomarker

Abstract Introduction Metabolomics is an essential tool for Translational Medicine, with a holistic non targeted approach, which provides information from the metabolic profile that can be used as a potential biomarker for a certain feature. The identification of biomarker profiles can be translated into simplified tests for the early diagnosis of diseases such as graft-versus-host disease (GVHD). Aim To evaluate the feasibility of a diagnostic protocol for chronic GVHD (cGVHD) based on the metabolic profile of blood serum from patients (pts) submitted to hematopoietic stem cell transplantation (HSCT). Methodology The study included a prospective, nested cohort of 41 adult pts who had undergone first full-match allogeneic HSCT at Hospital de Cl’nicas/ University of Campinas between August, 2011-January, 2013 and had signed the Consent Form. Blood serum samples were prospectively collected on the day of hospitalization (D-8), day of transplantation (D0), NADIR phase (D+10) and D+100 post-HSCT. The Nuclear Magnetic Resonance (NMR)-based metabolomic experiments were performed at the Brazilian Biosciences National Laboratory (LNBio/CNPEM). Samples were previously filtered on a 3 kDa cut off filter, diluted 3X on 100 mM phosphate buffer pH 7.4, referenced with 10% D2O and 0.5 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) for further absolute quantification of metabolites. The acquisition of 1D spectra was performed on a Agilent/Varian INOVA spectrometer operating at 1H resonance frequency of 600MHz. The spectra processing, identification and quantification of metabolites were performed by using the application package Chenomx NMR Suite and the target profiling approach (Chenomx, Inc). Samples from pts who developed chronic GVHD, at anytime, were marked as “cGVHD group”, and those who did not develop chronic GVHD, “cGVHD-free group” . Blood samples from pts who died up to 200 days after HSCT and did not develop chronic GVHD were excluded from the analysis, as were also all samples from pts whose sample collection was lacking at least in one period. T-tests (p<0.05) were applied at each sampling time in order to select metabolites and perform Principal Component Analysis (PCA), hierarchical clustering and calculate Receiver Operating Characteristic (ROC) curve for cGVHD diagnosis model using the software Pirouette¨ 4.0 (Infometrix, Inc) and the web tools MetaboAnalyst 2.0 and ROCCET. Results Median age was 41(18-69) years old and 61% were male. 18/41(44%) pts matched the inclusion criteria for the study. 6/18 (33%) pts had undergone cGVHD therapy. Seventy-three metabolites were identified and quantified in the blood serum samples. 8 metabolites were selected by T-test application at each studied time (2 metabolites from D-8, 2 from D0, 3 from D+10 and 1 from D+100). Using the selected metabolites the PCA showed clear separation between pts from “cGVHD” and “cGVHD-free” groups as well as hierarchical clustering (Fig. 1), confirmed by ROC analysis as a high sensitivity and specificity model for cGVHD diagnosis (AUC 0.99 and CI 0.93-1.00) (Fig. 2). Conclusion Metabolic differences were found in both studied groups. These differences may be used as a biomarker profile for cGVHD diagnosis up 100 days post-HSCT. Our results demonstrated that the statistical model using combined prospective time-series analysis is a powerful metabolomic model for early cGVHD diagnosis. Disclosures: Alborghetti: Sao Paulo Research Foundation: Research Funding. da Silva:Coordination for the Improvement of Higher Education Personnel (CAPES): Research Funding.

scholarly journals Comparison of Trace Elements Levels in Atrial Fibrillation Patients and Healthy Group

2020 ◽  
pp. 113-119
Author(s):  
Zahra Bazargani ◽  
Manzar Banoo Shojaeifard ◽  
Gholam Abbas Valizadeh
Keyword(s):  
Atrial Fibrillation ◽  
Trace Elements ◽  
Blood Serum ◽  
Atomic Absorption ◽  
Healthy Subjects ◽  
Statistical Analyses ◽  
Serum Samples ◽  
Blood Samples ◽  
Healthy Group ◽  
Significant Difference

Background: The aim of this study is to measure the concentration of Zinc (Zn), copper (Cu)  and selenium (Se) in the blood serum of Atrial Fibrillation (AF) patients as compared to healthy subjects in both genders. Methods: This study, conducted on patients with AF (n = 50) and controls (n = 24) were evaluated by available method. Blood samples were taken from the patients and analysis of trace elements was performed by atomic absorption spectrophotometer. Statistical analyses were done using IBM-SPSS 22.0. Results: Zn and Se concentrations were evaluated in AF patients and healthy group. Zn and Se concentration in AF patients is much lower than in healthy group (p<0.008, p<0.000). AF patients showed a significantly higher Cu concentration than the healthy group (p<0.000). The data of the present study revealed that the concentrations of all trace elements had a significant difference in the serum of AF patients with respect to the healthy group (p<0.05). Conclusion: We found that concentrations of Zn & Se levels were significantly decrease in the in the serum samples of AF patients compared with the controls group.

scholarly journals The influence of genetic provenience on metabolic blood profile of dairy cows

2011 ◽  
Vol 27 (3) ◽  
pp. 1025-1031 ◽  
Author(s):  
Z. Spasic ◽  
B. Milosevic ◽  
Z. Ilic ◽  
Lj. Andjusic ◽  
S. Ciric ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Total Bilirubin ◽  
Statistical Significance ◽  
Inorganic Phosphorus ◽  
Distribution Area ◽  
Physiological Status ◽  
Blood Profile ◽  
Analysis Of Results ◽  
Test Of Significance

Availability of easily accessible methods and apparatus for establishing the essential parameters that indicate physiological status of animals at different stages of productive life may have greater application in daily controls on smaller farms in order to remove, in time, the causes and consequences of disturbances in the reproductive and productive cycle of cows. Two groups were formed, Domestic spotted cattle and Red Holstein, and in the blood serum from tested animals following parameters were determined: glucose, urea, total bilirubin, aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), and inorganic phosphorus. Determined glucose concentration of 2,97 mmol/L in redholstein cows is within physiological range, while in domestic spotted cows it is in hypoglycemia (1,14 mmol/L). Statistical significance also demonstrate derived concentrations in different genotypes of blood urea (3,42 : 4,90 mmol/L) and GLDH (12,19 : 5,76 IU/L).Differences in concentrations of certain parameters of the examined blood serum, and variations amplitude that occur between groups, as well as statistical analysis of results and the test of significance, just confirm the hypothesis that for each animal breed, reference values related to narrow distribution area of growing should be established.

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