scholarly journals Quantification of mast cells in different stages of periodontal disease

2016 ◽  
Vol 73 (5) ◽  
pp. 458-462 ◽  
Author(s):  
Dragan Marjanovic ◽  
Zlatibor Andjelkovic ◽  
Zlata Brkic ◽  
Goran Videnovic ◽  
Meliha Sehalic ◽  
...  
Keyword(s):  
Mast Cells ◽  
Periodontal Disease ◽  
Mononuclear Cells ◽  
Inflammatory Cells ◽  
Biologically Active ◽  
Gingival Tissue ◽  
Blue Staining ◽  
Toluidine Blue Staining ◽  
Inflammatory Processes ◽  
Severe Periodontal Disease

Background/Aim. Mast cells are mononuclear cells originating from bone marrow. They produce various biologically active substances, which allow them to actively participate in immune and inflammatory processes associated with periodontal disease. The study focused on distribution and density of mast cells in healthy gingiva as well as in different stages of periodontal disease. Methods. The material used for this purpose was gingival biopsies taken from 96 patients classified into 4 groups: healthy gingiva, gingivitis, initial and severe periodontal disease. Toluidine blue staining according to Spicer was utilized for identifying mast cells. Results. Basing on our study, the density of mast cells in the gingival tissue increases with the progression of the infection, which means they are more numerous in gingivitis compared to healthy gingiva, as well as in periodontal disease compared to gingivitis. Conclusion. Increase in the number of mast cells in the infected gingiva can be correlated with an increased influx of inflammatory cells from blood circulation into the gingival stroma, as well as with the collagen lysis, since these cells produce substances with collagenolytic potential. Based on the distribution of mast cells, it could be concluded that in the evolution of periodontal disease there are significant dynamic alterations in migration and localization of these cells.

scholarly journals Morphometric analysis of collagen and inflammatory cells in periodontal disease

2015 ◽  
Vol 72 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Ranko Golijanin ◽  
Bojan Kujundzic ◽  
Zoran Milosavljevic ◽  
Dragan Milovanovic ◽  
Zlatibor Andjelkovic ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Morphometric Analysis ◽  
Plasma Cells ◽  
Relative Proportion ◽  
Inflammatory Cells ◽  
Test System ◽  
Clinical Staging ◽  
Gingival Tissue ◽  
Tissue Destruction ◽  
Immunochemical Method

Background/Aim. Periodontal disease affects gingival tissue and supporting apparatus of the teeth leading to its decay. The aim of this study was to highlight and precisely determine histological changes in the gum tissue. Methods. Gingival biopsy samples from 53 healthy and parodontopathy-affected patients were used. Clinical staging of the disease was performed. Tissue specimens were fixed and routinely processed. Sections, 5 ?m thin, were stained with hematoxylin and eosin, histochemical Van-Gieson for the collagen content, Spicer method for mast-cells and immunochemical method with anti-CD68 and anti-CD38 for the labelling of the macrophages and plasma-cells. Morphometric analysis was performed by a M42 test system. Results. While the disease advanced, collagen and fibroblast volume density decreased almost twice in the severe cases compared to the control ones, but a significant variation was observed within the investigated groups. The mast-cell number increased nearly two times, while the macrophage content was up to three times higher in severe parodontopathy than in healthy gingival tissue. However, the relative proportion of these cells stayed around 6% in all cases. Plasma-cells had the most prominent increase in the number (over 8 times) compared to the control, but again, a variation within investigated groups was very high. Conclusion. Gingival tissue destruction caused by inflammatory process leads to significant changes in collagen density and population of resident connective tissue cells. Although inflammatory cells dominated with the disease advancing, a high variation within the same investigated groups suggests fluctuation of the pathological process. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/VSP1704391E">10.2298/VSP1704391E</a><u></b></font>

scholarly journals Mast Cells May Regulate The Anti-Inflammatory Activity of IL-37

2019 ◽  
Vol 20 (15) ◽  
pp. 3701 ◽  
Author(s):  
Theoharis C. Theoharides ◽  
Irene Tsilioni ◽  
Pio Conti
Keyword(s):  
Mast Cells ◽  
Immune Cells ◽  
Therapeutic Agents ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Allergic Reactions ◽  
Regulatory Cytokine ◽  
Inflammatory Processes ◽  
Anti Inflammatory ◽  
Novel Therapeutic

Mast cells are unique immune cells involved in allergic reactions, but also in immunity and inflammation. Interleukin 37 (IL-37) has emerged as an important regulatory cytokine with ability to inhibit immune and inflammatory processes. IL-37 is made primarily by macrophages upon activation of toll-like receptors (TLR) leading to generation of mature IL-37 via the action of caspase 1. In this review, we advance the premise that mast cells could regulate the anti-inflammatory activity of the IL-37 via their secretion of heparin and tryptase. Extracellular IL-37 could either dimerize in the presence of heparin and lose biological activity, or be acted upon by proteases that can generate even more biologically active IL-37 forms. Molecules that could selectively inhibit the secretion of mast cell mediators may, therefore, be used together with IL-37 as novel therapeutic agents.

scholarly journals Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

2017 ◽  
Vol 28 (6) ◽  
pp. 679-687 ◽  
Author(s):  
Fabrício Gibertoni ◽  
Meire Ellen Ligia Sommer ◽  
Marcelo Augusto Marretto Esquisatto ◽  
Maria Esméria Corezola do Amaral ◽  
Camila Andrea de Oliveira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Bone Loss ◽  
Periodontal Disease ◽  
Immunohistochemical Analysis ◽  
Inflammatory Cells ◽  
Gingival Tissue ◽  
Cervical Region ◽  
Nitric Oxide Concentration ◽  
Gene Transcripts

Abstract The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-b1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.

scholarly journals Proliferative Effects of Histamine on Primary Human Pterygium Fibroblasts

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Zhenwei Qin ◽  
Qiuli Fu ◽  
Lifang Zhang ◽  
Houfa Yin ◽  
Xiuming Jin ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Receptor ◽  
Blue Staining ◽  
Toluidine Blue Staining ◽  
Ki67 Expression ◽  
Proliferative Effect ◽  
Diphenhydramine Hydrochloride ◽  
Human Pterygium Fibroblasts ◽  
Diphenyltetrazolium Bromide ◽  
And Migration

Purpose. It has been confirmed that inflammatory cytokines are involved in the progression of pterygium. Histamine can enhance proliferation and migration of many cells. Therefore, we intend to investigate the proliferative and migratory effects of histamine on primary culture of human pterygium fibroblasts (HPFs). Methods. Pterygium and conjunctiva samples were obtained from surgery, and toluidine blue staining was used to identify mast cells. 3-[4, 5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was performed to evaluate the proliferative rate of HPFs and human conjunctival fibroblasts (HCFs); ki67 expression was also measured by immunofluorescence analysis. Histamine receptor-1 (H1R) antagonist (Diphenhydramine Hydrochloride) and histamine receptor-2 (H2R) antagonist (Nizatidine) were added to figure out which receptor was involved. Wound healing model was used to evaluate the migratory ability of HPFs. Results. The numbers of total mast cells and degranulated mast cells were both higher in pterygium than in conjunctiva. Histamine had a proliferative effect on both HPFs and HCFs, the effective concentration (10 μmol/L) on HPFs was lower than on HCFs (100 μmol/L), and the effect could be blocked by H1R antagonist. Histamine showed no migratory effect on HPFs. Conclusion. Histamine may play an important role in the proliferation of HPFs and act through H1R.

scholarly journals A case report of primary cutaneous marginal zone B-cell lymphoma with mastocytosis

2021 ◽  
Vol 9 ◽  
pp. 2050313X2110425
Author(s):  
Hyun Yi Lee ◽  
Joong Sun Lee ◽  
Dae Won Koo
Keyword(s):  
Mast Cells ◽  
B Cell ◽  
Marginal Zone ◽  
Plasma Cells ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Blue Staining ◽  
Chain Gene ◽  
Toluidine Blue Staining ◽  
Chain Gene Rearrangement

A 53-year-old man presented with asymptomatic, dusky reddish nodules on his trunk, which had persisted for 7 years. Histological findings showed nodular to diffuse dermal infiltration of lymphocytes with irregular nuclei, eosinophils, plasma cells, and mast cells. CD20 revealed patch positivity. Periphery of lymphoid follicles showed BCL-2 positivity and BCL-6 positivity focally at the center. CD30 and toluidine blue staining showed positivity, and several mast cells were confirmed. The immunoglobulin heavy chain gene rearrangement result showed B-cell monoclonality. The patient’s condition was diagnosed as cutaneous marginal zone B-cell lymphoma, plasmacytoid type with mastocytosis. Primary cutaneous marginal zone B-cell lymphoma is an indolent lymphoma with a tendency for local recurrence. In the specimen obtained from our patient, multiple mast cells were observed. Primary cutaneous marginal zone B-cell lymphoma with prominent mastocytosis is rare and there are a few reported cases. Therefore, this case is worth reporting for its rarity and for the purpose of reminding mastocytosis in primary cutaneous marginal zone B-cell lymphoma.

scholarly journals Tryptase- and ghrelin positive mast cells in the interalveolar septa of rat’s lung

2021 ◽  
Vol 24 (4) ◽  
pp. 469-477
Author(s):  
I. G. Ivanova ◽  
I. S. Stefanov
Keyword(s):  
Mast Cells ◽  
Toluidine Blue ◽  
Experimental Studies ◽  
Left Lung ◽  
Lung Parenchyma ◽  
Blue Staining ◽  
Toluidine Blue Staining ◽  
Tissue Sections ◽  
Old Rats ◽  
Immunohistochemical Identification

The mast cell mediators and distribution of lung mast cells in rats are often discussed in experimental studies on pulmonary fibrotic and allergic processes associated with changes in numbers of these cells, but information on the normal distribution of metachromatic and tryptase-positive mast cells in the interalveolar septa is scarce. There are no data on the presence of ghrelin in lung mast cells as well as the age-specific features of localisation and the number of mast cells in the interalveolar septa in rats of different ages. Therefore, the purpose of the present study was to determine the distribution of metachromatic, tryptase-, and ghrelin-positive mast cells in the interalveolar septa in 20 day-, 3 month- and 1 year-old rats. Tissue sections stained with toluidine blue had been taken from the left lung to visualise metachromasia and immunohistochemical expression of tryptase and ghrelin. The results showed that the amount of metachromatic mast cells in the interalveolar septa was significantly lower than that of tryptase- and ghrelin-positive cells. This allowed suggesting that mast cells were permanent occupants of the rat lung parenchyma and, on the other hand, the expression of ghrelin in their granules was most likely related to the synthesis of this protein. Our study showed that immunohistochemical identification by tryptase expression was more accurate than toluidine blue staining.

scholarly journals Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining

2017 ◽  
pp. 213-222 ◽  
Author(s):  
Nahum Puebla-Osorio ◽  
Seri N. E. Sarchio ◽  
Stephen E. Ullrich ◽  
Scott N. Byrne
Keyword(s):  
Mast Cells ◽  
Toluidine Blue ◽  
Blue Staining ◽  
Toluidine Blue Staining

scholarly journals ICAM-1 expression on chondrocytes in rheumatoid arthritis: induction by synovial cytokines

1992 ◽  
Vol 1 (1) ◽  
pp. 71-74 ◽  
Author(s):  
M. E. Davies ◽  
H. Sharma ◽  
R. Pigott
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Cells ◽  
Intercellular Adhesion Molecule ◽  
Blue Staining ◽  
Human Articular Cartilage ◽  
Intercellular Adhesion Molecule 1 ◽  
Toluidine Blue Staining ◽  
Cartilage Extracellular Matrix ◽  
Normal Human

The intercellular adhesion molecule-1 (ICAM-1) was found by immunostaining chondrocytes in cartilage from three patients with rheumatoid arthritis. Expression of ICAM-1 was restricted to chondrocytes in areas of erodedcartilage adjacent to the invading synovial tissue. Toluidine blue staining of these areas demonstrated severe depletion of the cartilage extracellular matrix. In areas of undamaged cartilage there was no ICAM-1 expression. Since ICAM-1 is not constitutively expressed on normal human articular cartilage, but could be induced in vitro by exogenous IL-1α, TNFα and IFNγ or by co-culturing cartilage with inflammatory rheumatoid synovium, we conclude that the induction of ICAM-1 on rheumatoid chondrocytes results from the synergistic action of a variety of cytokines produced by the inflammatory cells of the invading pannus.

Quantitative Analysis of Tryptase- and Chymase-containing Mast Cells in Eosinophilic Conditions of Cats

2003 ◽  
Vol 40 (2) ◽  
pp. 219-221 ◽  
Author(s):  
C. Noli ◽  
M. Welle ◽  
F. Scarampella ◽  
F. Abramo
Keyword(s):  
Mast Cells ◽  
Eosinophilic Granuloma ◽  
Staining Technique ◽  
Mean Value ◽  
Blue Staining ◽  
Double Labeling ◽  
Toluidine Blue Staining ◽  
Biopsy Specimens ◽  
Superficial Dermis ◽  
First Time

The presence and density of tryptase-positive/chymase-positive mast cells (MCs) (MCTC), chymase-positive/tryptase-negative MCs (MCC), and tryptase-positive/chymase-negative MCs (MCT) in lesional skin from cats with eosinophilic conditions were investigated. Skin biopsy specimens from eight cats with eosinophilic plaque (three cats), eosinophilic granuloma (two cats), and eosinophilic dermatitis (three cats) were studied. Toluidine blue staining and a double-enzyme-immunohistochemical staining technique were performed to determine MC density and MC subtypes, respectively. MC density varied from 170.3 to 503 cells/mm2 (mean value of 314.9 cells/mm2). In the superficial dermis, 5.9% of the MC belonged to the MCT, 12.8% to the MCC, and 81.2% to the MCTC subtype. In the deep dermis, 12.8% belonged to the MCT, 12.8% to the MCC, and 73.8% to the MCTC subtype. It is the first time that MCC have been identified. The double-labeling procedure proved to be a reliable tool for identifying simultaneously the presence of MC subtypes in feline skin.

Sign in / Sign up

Export Citation Format

Share Document