scholarly journals Antifungal activity of cultivated oyster mushrooms on various agro-wastes

2017 ◽  
Vol 43 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Mustafa Nadhim Owaid ◽  
Sajid Salahuddin Saleem Al-Saeedi ◽  
Idham Ali Abed Al-Assaffii
Keyword(s):  
Antifungal Activity ◽  
Wheat Straw ◽  
Fungal Pathogens ◽  
Date Palm ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruiting Bodies ◽  
Disc Diffusion Method ◽  
Date Palm Fibers

ABSTRACT This study evaluated the antifungal activity of four fruiting bodies of oyster mushroom harvested from three agro-substrates in vitro. At three concentrations (2, 4 and 8 mg/disc), extracts discs of Pleurotus ostreatus (grey), P. ostreatus var. florida, P. cornucopiae var. citrinopileatus and P. salmoneostramineus were tested against three fungal pathogens: Trichoderma harzianum (after 2 days), Verticillium sp. and Pythium sp. (after 5 days) via the Disc Diffusion Method. The highest overall activity was by the extract disc Y2 (P. cornucopiae grown on M2 substrate; 70% wheat straw, 20% hardwood sawdust and 10% date palm fibers) and the lowest by Y1 (P. cornucopiae grown on wheat straw). The best inhibition zone was 16 mm toward T. harzianum by extract disc W2 (2 mg/disc) (P. ostreatus var. florida grown on M2 substrate), compared with 23 mm with Nystatin disc (100 U), followed 7 and 5 mm by P3 (P. salmoneostramineus grown on M3 substrate; 50% wheat straw, 30% hardwood sawdust and 20% date palm fibers) extract disc (8 mg/disc) against Pythium sp., and (4 mg/disc) against Verticillium sp., respectively.

scholarly journals Antifungal activity of Mentha piperita and Carum carvi essential oils

2017 ◽  
pp. 201-207 ◽  
Author(s):  
Dragana Plavsic ◽  
Gordana Dimic ◽  
Djordje Psodorov ◽  
Dragan Psodorov ◽  
Ljubisa Saric ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Aromatic Plants ◽  
Disc Diffusion Method ◽  
Penicillium Aurantiogriseum

Aromatic plants are one of the most important sources of biologically active secondary metabolites, which possess various antimicrobial characteristics. The aim of this work was to examine the effect of antifungal activities of mint and caraway essential oils against the selected fungi. Eight species of molds were selected for antifungal testing: Alternaria alternata, Aspegillus flavus, A. niger, A. versicolor, Eurotium herbariorum, Penicillium aurantiogriseum, P. chrysogenum and P. expansum. Testing of essential oils antifungal activity against the selected species was conducted using the disc diffusion method by adding mint and caraway essential oils (0.5, 1, 5, and 10 ?l per disc). Antifungal activity of essential oils was expressed by the diameter of inhibition zone (mm). The most powerful effect of mint essential oil was recorded against E. herbariorum, as its growth was completely inhibited by the quantity of 5 ?l. The weakest inhibitory effect was observed against P. chrysogenum (inhibition zone 13.67 mm) by the quantity of 10 ?l. The most powerful antifungal activity of caraway was observed against E. herbariorum as growth was completely inhibited by the quantity of 10 ?l. The weakest inhibitory effect was observed against A. niger (inhibition zone 28 mm) by the quantity of 10 ?l.

scholarly journals Mycelial growth observation of Pleurotus eryngii (Higher Basidiomycota) In Vitro

2016 ◽  
Vol 5 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Mustafa Nadhim Owaid ◽  
Sajid SS Al-Saeedi ◽  
Idham Ali Abed
Keyword(s):  
Wheat Straw ◽  
Mycelial Growth ◽  
Date Palm ◽  
Pleurotus Eryngii ◽  
Intensity Level ◽  
Palm Fiber ◽  
Date Palm Fibers ◽  
King Oyster Mushroom ◽  
Vigorous Growth

Five agro-substrates including date palm fibers (fibrillum), wheat straw, white sawdust and their combinations were investigated to grow Pleurotus eryngii. The longer mycelium complete time within bags was 20 days on sawdust (S4), in contrast, the shorter time for mycelium overgrew was completed after 15 days on date palm fiber (S5). In significant (p<0.05), S5 showed the higher growth intensity level (vigorous growth) than other substrates. Thus use of date palm wastes (S5 medium) may be useful for successfully cultivation king oyster mushroom in farm.International Journal of Environment Vol.5(3) 2016, pp.1-10

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

scholarly journals Synthesis of Novel Chitin Derivatives Bearing Amino Groups and Evaluation of Their Antifungal Activity

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 380
Author(s):  
Jingjing Zhang ◽  
Fang Luan ◽  
Qing Li ◽  
Guodong Gu ◽  
Fang Dong ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Amino Groups ◽  
Disk Diffusion Method ◽  
Elemental Analyses ◽  
Ft Ir ◽  
Chitin Derivatives

Chemical modification is one of the most effective methods to improve the biological activity of chitin. In the current study, we modified C3-OH and C6-OH of chitin (CT) and successfully synthesized 6-amino-chitin (NCT) and 3,6-diamino-chitin (DNCT) through a series of chemical reactions. The structure of NCT and DNCT were characterized by elemental analyses, FT-IR, 13C NMR, XRD, and SEM. The inhibitory effects of CT, NCT, and DNCT against six kinds of phytopathogen (F. oxysporum f. sp. cucumerium, B. cinerea, C. lagenarium, P. asparagi, F. oxysporum f. niveum, and G. zeae) were evaluated using disk diffusion method in vitro. Meanwhile, carbendazim and amphotericin B were used as positive controls. Results revealed that 6-amino-chitin (NCT) and 3,6-diamino-chitin (DNCT) showed improved antifungal properties compared with pristine chitin. Moreover, DNCT exhibited the better antifungal property than NCT. Especially, while the inhibition zone diameters of NCT are ranged from 11.2 to 16.3 mm, DNCT are about 11.4–20.4 mm. These data demonstrated that the introduction of amino group into chitin derivatives could be key to increasing the antifungal activity of such compounds, and the greater the number of amino groups in the chitin derivatives, the better their antifungal activity was.

Biological evaluation of 10-(diphenylmethylene)- 4-azatricyclo[5.2.1.02,6]dec-8-ene-3,5-dione derivatives

2009 ◽  
Vol 4 (3) ◽  
pp. 362-368 ◽  
Author(s):  
Joanna Stefańska ◽  
Anna Bielenica ◽  
Marta Struga ◽  
Stafan Tyski ◽  
Jerzy Kossakowski ◽  
...  
Keyword(s):  
Agar Medium ◽  
Inhibition Zone ◽  
Biological Evaluation ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal Activity ◽  
Disc Diffusion Method ◽  
Growth Inhibition Zone ◽  
Antibacterial And Antifungal

AbstractAntibacterial and antifungal activity of 10-(diphenylmethylene)-4-azatricyclo[5.2.1.02,6]dec-8-ene-3,5-dione derivatives were examined by the disc-diffusion method (growth inhibition zone diameter in agar medium). The MIC’s for the most active agents were determined. Title compounds were also evaluated in vitro against representatives of different virus classes. Most of the tested compounds exhibit activity against CVB-2 virus.

In vitro anti-fungal activity of few medicinal plants of Visakhapatnam region against clinically isolated dermatophytes

2016 ◽  
Vol 5 (05) ◽  
pp. 4573
Author(s):  
Phani Kumari Uddandapu* ◽  
K. Chandrasekhara Naidu ◽  
Y. Venkateswar Rao
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Fungal Pathogens ◽  
Trichophyton Mentagrophytes ◽  
Diffusion Method ◽  
Bioactive Natural Products ◽  
South Indian ◽  
Ethnobotanical Uses ◽  
Traditional Indian Medicine

The aim of the study was to evaluate the antifungal activity of methanol extracts of twenty plant species used in traditional Indian medicine against the clinically isolated fungi. The plants were selected on the basis of their reported ethnobotanical uses. The studies on plants of South Indian medicinal plants collected from regions of Visakhapatnam and were investigated against three clinical fungal isolates viz., Trichophyton mentagrophytes, Epidermophyton floccosum and Candida albicans using agar well diffusion method. The plant extracts were prepared using the solvent methanol. It is clear from the results that, the extract of twenty plants used in this study acts as a good source of antibiotics against various fungal pathogens tested and exhibited a broad spectrum of antifungal activity. The results of this study support the use of all the selected twenty medicinal plants to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals that address unmet therapeutic needs.

scholarly journals Bactericidal And Antioxidant Effects of Domestic Essential Oils On Certain Pathogenic Bacteria.

2021 ◽  
Author(s):  
Faisal MB. Al-Sarraj ◽  
Ahmad M. Qumri
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Rapd Pcr

Abstract Background: Essential oils (EOs) are volatile, liquid substances that come from natural sources such as plants. As secondary metabolites of most plant species, that are typically found in leaf chloroplasts or from the hydrolysis of certain glycosides, their primary function is to combat attacks from predators as well as attract pollinators. Due to their biological activity, EOs have gained popularity in industries such as food processing, cosmetics, and personal care products as well as the pharmaceutical industry. In this study, the antimicrobial nature and antioxidant activity of Saudi Arabian EOs were compared with the antimicrobial activity of known antibiotics.Methods: The In vitro evaluation of antibacterial activity and antioxidant capacity of EOs collected from Jeddah domestic market for gram-negative and gram-positive pathogenic bacteria was carried out using the disc diffusion method. Disc diffusion method was used for the assessment of bactericidal activity of local EOs. The antioxidant activity was determined using phosphomolybdenum method. Furthermore, RAPD-PCR was used to compare the effects of these EOs on DNA in both untreated and treated pathogenic bacterial strains.Results and Conclusion: Bacterial strains with varying antibiotic resistance potential have been successfully isolated. The inhibition zone measurement revealed that the EOs used had some antibacterial effects on the bacterial strains as well as some selectivity on the bacterial strains that they acted on.

AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 12-19
Author(s):  
Oom Komala ◽  
Yulianita . ◽  
Fuji Raka Siwi
Keyword(s):  
Antifungal Activity ◽  
Ethanol Extract ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Lawsonia Inermis ◽  
Disc Diffusion Method ◽  
Mic Value

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.

scholarly journals Antimicrobial and Antiviral Screening of Novel Indole Carboxamide and Propanamide Derivatives

2008 ◽  
Vol 63 (3-4) ◽  
pp. 189-195 ◽  
Author(s):  
Süreyya Ölgen ◽  
Nurten Altanlar ◽  
Ersin Karataylı ◽  
Mithat Bozdayı
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Activity Patterns ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Indole Derivatives

A few series of indole derivatives were screened for antimicrobial, antifungal and anti- HBV activities. The compounds were tested for their in vitro antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and for their antifungal activity against Candida albicans using a disc diffusion method, which measures the diameter of the inhibition zone around a paper disc soaked in a solution of the test compounds. The antimicrobial activity results showed that all compounds are as a active as the standard compound ampicillin against Staphylococcus aureus. It was also found that indole carboxamide derivatives, substituted at 3-position with several benzyl groups, showed better inhibition of Bacillus subtilis than their congeners substituted at 2-position. Activity patterns of the compounds against Escherichia coli and Staphylococcus aureus were found slightly different by the same method. In this case, there was no correlation between structure and activity of the compounds. The antifungal activity of carboxamide derivatives was found higher compared to that of the propanamide derivatives. The minimum inhibitory concentration (MIC) values of some indole derivatives were also determined by the tube dilution technique. The MIC values of the compounds were found nearly 20- to 100-fold smaller compared to the standard compounds ciprofloxacin and ampicillin (1.56-3.13 μg/ml and 1.56-12.5 μg/ml, respectively) against Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The MIC values of the tested compounds showed that these are better inhibitors for Candida albicans. Indole derivatives were screened by the anti-HBV susceptibility test. No compound showed good inhibition against the HBV virus

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

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