Immunohistochemical Analysis of Keratin Distribution in Clear Cell Syringoma.

1994 ◽  
Vol 56 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Shinichi SATO ◽  
Michiyo MARUYAMA ◽  
Kiyoshi TODA ◽  
Shinichi WATANABE
Keyword(s):  
Clear Cell ◽  
Immunohistochemical Analysis

scholarly journals Clear Cell Carcinomas of the Mullerian System: Does the Pathogenesis Vary Depending on Their Nuclear Grade and Their Association with Endometriosis? An Immunohistochemical Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ahmad Alduaij ◽  
Katrine Hansen ◽  
Tahreem A. Karim ◽  
Cunxian Zhang ◽  
Michelle M. Lomme ◽  
...  
Keyword(s):  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Nuclear Grade ◽  
High Grade ◽  
Ki 67 ◽  
High Nuclear Grade ◽  
Positive Immunostaining

Clear cell carcinomas (CCC) of the mullerian system are considered high grade tumors, but morphologically, the cells of CCC show both low and high grade features. The aims of the current study were to categorize CCC into low and high nuclear grade types, correlate their association with endometriosis, and then observe possible variations in pathogenesis based on their expression of p53 and Ki-67. We studied 41 pure mullerian CCCs and designated each as either a high (HNG) or low (LNG) nuclear grade tumor. Morphologically, 17 (41%) CCCs were LNG and 24 (59%) were HNG. Nine (38%) HNG and 2 (12%) LNG tumors showed positive immunostaining with p53. Endometriosis was associated with 8 (47%) LNG tumors and 8 (33%) HNG CCCs. Of the 11 cases with p53 alteration, 4 (1 LNG and 3 HNG) were associated with endometriosis. Conclusions: HNG CCCs, irrespective of their association with endometriosis, have alterations of p53. In general, LNG ovarian and endometrial CCCs, irrespective of their association with endometriosis/adenomyosis, are less likely to show p53 alteration. It appears that mullerian CCCs may have variable pathogenesis depending on their nuclear grade and association with endometriosis. A larger study is needed to validate these findings.

Endometrial Serous Carcinoma With Clear-Cell Change: Frequency and Immunohistochemical Analysis

2017 ◽  
Vol 26 (2) ◽  
pp. 126-134 ◽  
Author(s):  
Nosaibah Hariri ◽  
Morad Qarmali ◽  
Oluwole Fadare
Keyword(s):  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Serous Carcinoma ◽  
Case Group ◽  
Clear Cells ◽  
Napsin A ◽  
Significant Difference ◽  
Group 3 ◽  
Clear Cell Change ◽  
Group 1

The diagnostic distinction between endometrial serous carcinoma (ESC) and endometrial clear-cell carcinoma (CCC) may occasionally be problematic, and one potentially contributing factor is the finding of clear cells in otherwise classic cases of ESC. This study aimed to define the frequency of this finding and comparatively assessed the immunophenotype of the clear cells. A review of 56 cases of ESC identified 8 (14.28%) with clear cells, representing 1% to 20% (median 7.5) of tumoral volume in these cases. In only 3 cases were clear cells discernible at low (×20) magnification. There was no significant difference in stage distribution or age between ESC patients with and without clear cells. The immunophenotypes of ESC-associated clear cells (group 1) were compared with foci of conventional ESC on another tissue block within the same case (group 2; n = 8) as well as a randomly selected cohort of CCC cases (group 3; n = 8). Groups 1 and 2 showed no significant differences regarding p53, ER, PR, Napsin-A, p504S, and hepatocyte nuclear factor 1β (HNF1β) expression, or regarding mitotic indices or Ki67 proliferation rate. In contrast, group 1 cases showed an immunophenotypic profile that was notably different from that of group 3 cases, with the former showing statistically significantly higher/more frequent expression of ER, PR, Ki67, and p53 and lower/less frequent expression of Napsin-A, p504S, and HNF1β. We conclude that clear-cell change is seen in 14% of ESCs and is discernible at low magnification in only 5%; these areas show an immunophenotype that is essentially identical to the associated background conventional ESC and are phenotypically dissimilar to CCC.

scholarly journals Intramedullary clear cell ependymoma of the lower thoracic spinal cord: report of a new case

2020 ◽  
Vol 11 ◽  
pp. 423
Author(s):  
Jesus Rocha-Maguey ◽  
Jesus Salvador Velarde-Felix ◽  
Myriam Cabrera-Lopez ◽  
Jaime Moya-Nuñez ◽  
Edgar Fragosa-Sanchez
Keyword(s):  
Differential Diagnosis ◽  
Clear Cell ◽  
Case Reports ◽  
Immunohistochemical Analysis ◽  
Gross Total Resection ◽  
Neurophysiological Monitoring ◽  
Total Resection ◽  
Thoracic Spinal Cord ◽  
Thoracic Spinal ◽  
Immunohistochemical Techniques

Background: Clear cell ependymomas (CCEs) are a rare variant of tumors of the nervous system, the main location is the intracranial compartment. Special differential diagnosis should be done with oligodendrogliomas, neurocytoma, glioneurocytoma, astrocytoma, or metastatic renal cell carcinoma, lesions that somehow share cells with clear cytoplasm. Most of these lesions are benign but differential diagnosis is essential to decide further treatment. Few case reports of intramedullary CCEs have being published and there is no strict consensus on the diagnostic criteria. Case Description: We hereby describe a new case of an intramedullary clear CCE with very few neurological symptoms, surgical treatment is satisfactory, histological and immunohistochemical analysis was confirmatory. After gross total resection and 3-year follow-up no recurrence of the lesion is evident. Conclusion: After this case presentation and review of the limited literature, it is evident that methodical clinical suspicion, radiological imaging combined with histological, and modern immunohistochemical techniques are essential for the diagnosis. Surgical options with gross total resection remain the cornerstone of its treatment. Neurophysiological monitoring is extremely useful to avoid postoperative morbidity.

scholarly journals FCER1G Positively Relates To Macrophage Infiltration In Clear Cell Renal Cell Carcinoma And Contributes To Unfavorable Prognosis By Regulating Tumor Immunity

2021 ◽  
Author(s):  
Keqin Dong ◽  
Wenjin Chen ◽  
Xiuwu Pan ◽  
Hongru Wang ◽  
Ye Sun ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
T Cell ◽  
Differentially Expressed Genes ◽  
Renal Cell ◽  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Differentially Expressed ◽  
High Expression ◽  
Cell Renal Cell Carcinoma ◽  
Tumor Tissues

Abstract Background: Tumor-associated macrophages (TAMs) are closely related to unfavorable prognosis of patients with clear cell renal cell carcinoma (ccRCC). However, the important molecules in the interaction between ccRCC and TAMs are unclear.Methods: TCGA-KIRC gene expression data of tumor tissues and normal tissues adjacent to tumor were compared to identify differentially expressed genes in ccRCC. TAMs related genes were discovered by analyzing the correlation between these differentially expressed genes and common macrophage biomarkers. Gene set enrichment analysis was performed to predict functions of TAMs related gene. The findings were further validated using RNA sequencing data obtained from the CheckMate 025 study and immunohistochemical analysis of samples from 350 patients with ccRCC. Kaplan–Meier survival curve, Cox regression analysis and Harrell’s concordance index analysis were used to determine the prognostic significance.Results: In this study, we applied bioinformatic analysis to explore TAMs related differentially expressed genes in ccRCC and identified 5 genes strongly correlated with all selected macrophage biomarkers: STAC3, LGALS9, TREM2, FCER1G, and PILRA. Among them, FCER1G was abundantly expressed in tumor tissues and showed prognostic importance in patients with ccRCC who received treatment with Nivolumab; however, it did not exhibit prognostic value in those treated with Everolimus. We also discovered that high expression levels of FCER1G are related to T cell suppression. Moreover, combination of FCER1G and macrophage biomarker CD68 can improve the prognostic stratification of patients with ccRCC from TCGA-KIRC. Based on the immunohistochemical analysis of samples from patients with ccRCC, we further validated that FCER1G and CD68 are both highly expressed in tumor tissue and correlate with each other. Higher expression of CD68 or FCER1G in ccRCC tissue indicates shorter overall survival and progression-free survival; patients with high expression of both CD68 and FCER1G have the worst outcome. Combining CD68 and FCER1G facilitates the screening of patients with a worse prognosis from the same TNM stage group.Conclusions: High expression of FCER1G in ccRCC is closely related to TAMs infiltration and suppression of T cell function. Combining the expression levels of FCER1G and macrophage biomarker CD68 may be a promising postoperative prognostic index for patients with ccRCC.

scholarly journals Clear cell chondrosarcoma in Von Hippel-Lindau disease

2019 ◽  
Vol 19 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Koen M. A. Dreijerink ◽  
Rachel S. van Leeuwaarde ◽  
Wenzel M. Hackeng ◽  
Rachel H. Giles ◽  
Wendy W. J. de Leng ◽  
...  
Keyword(s):  
Loss Of Heterozygosity ◽  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Tumor Grade ◽  
Suppressor Gene ◽  
Clear Cell Chondrosarcoma ◽  
Von Hippel Lindau ◽  
Vhl Protein ◽  
Von Hippel Lindau Disease ◽  
A Cell

Abstract A diagnosis of clear cell chondrosarcoma of the ulna was made in a patient with Von Hippel-Lindau disease (VHL). After surgery, genetic analysis of the tumor tissue showed loss of heterozygosity at the VHL gene locus. Immunohistochemical analysis confirmed loss of expression of the VHL protein in the tumor cells. In addition, abundant Cyclin D1 expression in the tumor was observed. Chondrosarcoma has been described before in a VHL patient and VHL protein expression has been correlated to tumor grade in a series of sporadic chondrosarcomas. In this report, we show that clear cell chondrosarcoma may be a rare but canonical VHL manifestation through a cell-autonomous mechanism involving somatic loss-of-heterozygosity of the VHL tumor suppressor gene. We discuss the relevance of this observation with regard to the pathogenesis of clear cell chondrosarcoma in the context of VHL.

scholarly journals Quantitative Immunohistochemical Analysis of Matrilysin 1 (MMP-7) in Various Renal Cell Carcinoma Subtypes

2007 ◽  
Vol 20 (4) ◽  
pp. 697-705 ◽  
Author(s):  
E. Patraki ◽  
M.R. Cardillo
Keyword(s):  
Differential Expression ◽  
Spindle Cell ◽  
Clear Cell ◽  
Collecting Duct ◽  
Immunohistochemical Analysis ◽  
Tumor Grade ◽  
Renal Tissue ◽  
High Stage ◽  
Matrix Metalloproteinase 7

The role of matrilysin 1 or matrix metalloproteinase-7 (MMP-7) in cancer is extremely complex and poorly understood. In this study we investigated differential expression of MMP-7 in the epithelium and stroma of 95 paraffin-embedded renal tumor samples by immunohistochemistry and compared tumoral with normal peritumoral renal tissue. We also determined a possible correlation of the immunohistochemical findings with histological subtype, tumor grade and stage of RCC. In all areas examined MMP-7 protein expression was significantly higher in epithelium than in stroma (P<.01). MMP-7 was more less expressed in peritumoral normal areas than in benign epithelial neoplasias (renal papillary and oncocytomas) and RCC carcinomas, reaching the highest immunopositive reaction in chromophobe RCC subtypes, followed by conventional clear-cell and chromophilic-papillary RCC histological subtypes and the lowest levels in more aggressive RCC histotypes (spindle-cell and collecting-duct RCCs). MMP-7 reached their highest levels in high-grade and high-stage RCCs. Our observation suggests an important role of MMP-7 in the development and progression of renal cancer. The differential expression of MMP-7 in the various histological RCC subtypes may reflect the malignant phenotype and more aggressive behavior of RCCs.

Immunohistochemical Analysis of Clear Cell Carcinoma of the Gynecologic Tract

2001 ◽  
Vol 20 (3) ◽  
pp. 252-259 ◽  
Author(s):  
Russell Vang ◽  
Bonnie P. Whitaker ◽  
Anwar I. Farhood ◽  
Elvio G. Silva ◽  
Jae Y. Ro ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Clear Cell ◽  
Clear Cell Carcinoma ◽  
Immunohistochemical Analysis

scholarly journals Is there any potential link among caspase-8, p-p38 MAPK and bcl-2 in clear cell renal cell carcinomas? A comparative immunohistochemical analysis with clinical connotations

2009 ◽  
Vol 4 (1) ◽  
pp. 7 ◽  
Author(s):  
Vassilis Samaras ◽  
Maria Tsopanomichalou ◽  
Angeliki Stamatelli ◽  
Christos Arnaoutoglou ◽  
Efstathios Samaras ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Renal Cell ◽  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Caspase 8 ◽  
Renal Cell Carcinomas ◽  
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