scholarly journals Deteksi Asam Mefenamat pada Jamu Pegel Linu yang beredar di Wilayah Pekalongan

2020 ◽  
pp. 51-60
Author(s):  
Siska Rusmalina ◽  
Kharismatul Khasanah ◽  
Denny Kurniawan Nugroho
Keyword(s):  
Mefenamic Acid ◽  
Mobile Phase ◽  
Uv Light ◽  
Descriptive Study ◽  
Comparison Standard ◽  
Exclusion Criteria ◽  
Three Samples ◽  
Rf Values ◽  
Layer Chromatography ◽  
Positive Results

Jamu Pegel Linu is one of the most popular herbs in Indonesia and oftenly the producers added medicinal chemicals (BKO) so that the resulting therapeutic effect is more effective. One of the BKO added is mefenamic acid. The added content of mefenamic acid can cause harmful side effects such as seizures and can even cause coma. This study aimed to detect the BKO content of mefenamic acid in Jamu Pegel Linu distributed in Pekalongan region. This research was a descriptive study with purposive sampling. The sample used was all Jamu pegel linu that were distributing in Pekalongan region that were included the inclusion and exclusion criteria. The analytical method used is Thin Layer Chromatography (TLC) with a mobile phase in the form of ethyl acetate: methanol: ammonia (80:10:10). Spotting detection was carried out under UV light at λ 254 nm. The results of the spots obtained were compared with a comparison standard. To ensure the results obtained are confirmed using the color reaction method. The results of testing of 27 samples by TLC method obtained there are 3 (three) samples of herbal medicine giving positive results indicated by the similarity of rf values with comparative standards. The results obtained were tested qualitatively with color reagents to strengthen the results.

Improved one-dimensional thin-layer chromatography of phospholipids in amniotic fluid.

1982 ◽  
Vol 28 (1) ◽  
pp. 209-211 ◽  
Author(s):  
A A Pappas ◽  
R E Mullins ◽  
R H Gadsden
Keyword(s):  
Amniotic Fluid ◽  
Mobile Phase ◽  
Distress Syndrome ◽  
Extraction Procedure ◽  
Fetal Lung ◽  
One Dimensional ◽  
Cupric Acetate ◽  
Rf Values ◽  
Layer Chromatography ◽  
Lung Maturity

Abstract With this extraction procedure and chromatographic method, six amniotic fluid phospholipids [phosphatidylcholine (lecithin), sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol] are completely resolved in less than 70 min from the time the specimen is received. The mobile phase (chloroform/petroleum ether/methanol/acetic acid, 5/3/1.6/1, by vol) is used with commercially available 7.5 X 10 cm silica-gel plates. The phospholipids are made visible by immersing the plate in cupric acetate/phosphoric acid. Lecithin/sphingomyelin (L/S) ratios are determined densitometrically. Rf values are well reproducible for both samples and standards, as are L/S ratios, and the method is simple and inexpensive. The relation between L/S ratios (for 100 specimens of amniotic fluid measured by this method) and fetal status is consistent with other reports. We confirm phosphatidylglycerol to be a sensitive indicator of fetal lung maturity: when it was detectable in amniotic fluid, the newborn invariably showed no symptoms of the respiratory distress syndrome.

scholarly journals Antibacterial Activity of Leaves and Rhizome of Curcuma xanthorrhiza Essential Oils on Different Distillation Time

2018 ◽  
Vol 3 (3) ◽  
pp. 116-120
Author(s):  
Nur Syafiqah Nadiah Mohammad Rafi ◽  
Irmanida Batubara ◽  
Anthony Nyangson Steven
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Mobile Phase ◽  
Clear Zone ◽  
Rf Values ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography ◽  
And Staphylococcus Aureus

This research aims to determine the antibacterial activity of Curcuma xanthorrhiza leaves and rhizomes essential oil with different distillation time of 2 hours, 4 hours and 6 hours against Escherichia coli and Staphylococcus aureus. The essential oils produced then separated by thin layer chromatography (TLC) with n-hexane: toluene: dichloromethane 1:8:1 as the mobile phase. The result shows that on the first two hours distillation gave the highest yield compared to the next two hours or four hours. Based on TLC chromatograms, the highest content of compounds found at 4-6 hours distillation time. TLC bioautography contact was used for antibacterial activity tested on chromatogram and the clear zone appears on it indicates the spots were active as antibacterial activity against both bacteria with Rf values of 0.35 and 0.49 for rhizomes, rhizome essential oils 4-6 hours distillation time as the most significance active.

European Taxa of Saxicolous Lecidella Containing Chloroxanthones:Identification of Patterns Using Thin Layer Chromatography

1992 ◽  
Vol 24 (4) ◽  
pp. 383-397 ◽  
Author(s):  
Ch. Leuckert ◽  
J.-G. Knoph
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glacial Acetic Acid ◽  
Uv Light ◽  
Rf Values ◽  
Layer Chromatography ◽  
Spray Reagents

AbstractA method using TLC to recognize compound patterns consisting almost entirely of chloroxanthones in European saxicolous species of Lecidella is described. These are necessary for diagnosis of the taxa studied and of their chemotypes. Two solvents with complementary separatingqualities were required: C, toluene-glacial acetic acid (100:15); J, dichloromethane-acetone (4:1). Although the Rf values in solvent J strongly depend on the concentration of the substances, it is very suitable for identification because the xanthones in this system reveal distinct and characteristic fluorescence colours in UV light, simplifying interpretation enormously. No spray reagents are used. Identifications are made by co-chromatography with authentic lichencompounds

scholarly journals Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions

2017 ◽  
Vol 2017 ◽  
pp. 1-6
Author(s):  
Katarzyna Bober
Keyword(s):  
Mobile Phase ◽  
Uv Light ◽  
Degradation Products ◽  
Light Exposure ◽  
Accelerated Aging ◽  
External Factors ◽  
The Subject ◽  
The Impact ◽  
Layer Chromatography ◽  
Standard Solutions

The subject of durability of drugs is a very important problem investigated by researches. The method of accelerated aging is very often used for stability testing. It involves the influence of temperature, humidity, and light exposure. The aim of this work is estimation of contents of diphenhydramine in its standard solutions undergoing the impact of external factors, that is, temperature and UV light. The standard solutions of diphenhydramine were prepared in distilled water. The analysis of contents of compound investigated was carried out by use of HPTLC. Adsorption thin layer chromatography was performed on aluminum HPTLC plates precoated with silica gel 60F254 using mixture of ammonia, methanol, and ethyl acetate as mobile phase. The solutions investigated were exposed to UV light as well as being heated in 40°C. The contents of diphenhydramine were measured in initial solution and during experiment. The additional peaks on densitograms as well as changes in color of solution were observed as a result of exposure to UV light which can give information about new substances, degradation products of diphenhydramine, which were created during experiment.

scholarly journals Simultaneous Densitometric Determination of Indomethacin and Its Degradation Products, 4-Chlorobenzoic Acid and 5-Methoxy-2-Methyl-3-Indoleacetic Acid, in Pharmaceutical Preparations

2001 ◽  
Vol 84 (6) ◽  
pp. 1703-1707 ◽  
Author(s):  
Jan Krzek ◽  
Magorzata Starek
Keyword(s):  
Detection Limit ◽  
Thin Layer ◽  
Mobile Phase ◽  
Indoleacetic Acid ◽  
Degradation Products ◽  
Pharmaceutical Preparations ◽  
Chlorobenzoic Acid ◽  
Rf Values ◽  
Layer Chromatography

Abstract A densitometric method was developed for the identification and determination of indomethacin and its degradation products, 4-chlorobenzoic acid and 5-methoxy-2-methyl-3-indoleacetic acid, in pharmaceuticals. To separate these compounds, silica gel-coated thin-layer chromatography plates and the following mobile phase were used: 2-propanol–25% ammonia–water (8 + 1 + 1, v/v). UV densitometric measurements were made by comparing the absorption spectra and Rf values of appropriate standards with the pharmaceutical preparations examined. The conditions for separation were established and a low detection limit was obtained. Average recoveries were 100.69, 90.09, and 91.17% for indomethacin, 4-chlorobeznzoic acid, and 5-methoxy-2-methyl-3-indoleacetic acid, respectively.

Reverse Phase Thin Layer Chromatography of Some Aroclors, Halowaxes, and Pesticides

1973 ◽  
Vol 56 (2) ◽  
pp. 367-372
Author(s):  
David L Stalling ◽  
James N Huckins
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Gas Liquid Chromatography ◽  
Chlorinated Pesticides ◽  
Reverse Phase ◽  
Mobile Phases ◽  
Rf Values ◽  
Solvent Systems ◽  
Layer Chromatography

Abstract The components of Aroclors® 1232, 1248, 1254, and 1260; Halowaxes® 1099, 1013, and 1014; and several chlorinated pesticides are resolved by reverse phase thin layer chromatography (RPTLC), which permits component separation by partition between a nonpolar stationary phase and a polar mobile phase. Rf values of resulting spots were calculated for 2 of 4 new solvent systems (mobile phases). RPTLC patterns were reproducible and characteristic of each material examined. The spots were recovered from the plates and characterized by gas-liquid chromatography (GLC) and/or GLC-mass spectrometry. In some cases, single GLC peaks of Aroclor standards were resolved into more than one component by RPTLC, whereas some RPTLC spots of Halowaxes were resolved into as many as 4 GLC peaks. The analysis of environmental residues of chlorinated compounds was facilitated by this technique.

scholarly journals Identifikasi Senyawa Metabolit Sekunder Ekstrak Etanol Kulit Buah Pisang Kepok (Musa paradisiaca L) dengan Metode KLT

2019 ◽  
Vol 5 (2) ◽  
pp. 125
Author(s):  
Hasma Hasma ◽  
Winda Winda
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Uv Light ◽  
Banana Peel ◽  
Banana Plant ◽  
Chemical Reagents ◽  
Musa Paradisiaca ◽  
Rf Values ◽  
Layer Chromatography ◽  
Peel Extract

Indonesia is a country that is rich in abundant natural plants by having various types of plants which have medicinal properties and are used by some people as traditional medicines. One use of traditional medicine used by the community is the banana plant. So far the community only uses bananas, limited to the use of their fruit, then the banana peel after consumption is only disposed of as waste that is not used optimally by the community. This study aims to identify the chemical compounds found in the skin of Kepok Banana (Musa paradisiaca L) taken from the pioneer road in the city of Makassar. This identification is carried out using the Thin Layer Chromatography (TLC) method and chemical reagents. Kepok banana peel extract (Musa paradisiaca L.) is made using maceration method, because the method is not only simple, the equipment used is simple, easy to work on, can also avoid damage to compound components due to heat. The yield of Rendamen obtained was 12.06%. Identification was done using Silica Thin Layer Chromatography GF254 with N-hexane eluent: ethyl acetate (6: 4) eluents obtained 5 stains on 366 nm UV light, namely alkaloids with orange blotches with Rf value (0.13), saponins with blemishes. purple Rf value (0.92), flavonoids with yellow spots Rf values ​​(0.33) and tannins with blackish orange patches Rf values ​​(0.65). Based on testing using chemical reagents obtained positive results saponins, flavonoids, tannins and alkaloids. The conclusion of the study showed that the Kepok banana peel extract taken on the pioneering road of Makassar city contained alkaloids, saponins, flavonoids and tannins.

scholarly journals HPTLC analysis of berberine in stem extracts of Fibraurea darshani

2018 ◽  
Vol 7 (2) ◽  
pp. 2021
Author(s):  
Sheema Dharmapal ◽  
Bindu T.K. ◽  
Elyas K.K.
Keyword(s):  
High Performance ◽  
Uv Light ◽  
Solvent System ◽  
Plant Sample ◽  
Phytochemical Analysis ◽  
Methanolic Extracts ◽  
Uv Reflectance ◽  
The Family ◽  
Rf Values ◽  
Layer Chromatography

The present study is a first report on the phytochemical analysis of the plant Fibraurea darshani which is endemic to Western Ghats. The plant is a woody dioecious climber belonging to the family Menispermaceae. Preliminary phytochemical screening of methanolic extracts of the stem of F. darshani revealed the presence of secondary metabolites like alkaloids, carbohydrates, anthraquiones, terpenoids, flavonoids, phenolics, sterols etc. A simple and reproducible high performance thin layer chromatography was developed to evaluate the presence of berberine in methanol extract of stem of F. darshani. This method involves separation of compounds by HPTLC on pre-coated silica gel 60F 254 plates with a solvent system of Chloroform: Ethyl acetate: Methanol: Formic acid (4:5:4:0.3) and scanned using densitometric scanner in UV reflectance photo mode at 254 and 366nm. The Rf values (0.97) for berberine in the plant sample and the reference standard were found comparable under UV light at 366nm. The HPTLC method developed was simple, accurate and specific.

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