scholarly journals PENGARUH EKSTRAK RIMPANG JAHE (ZINGIBER OFFICINALE ROSC.) TERHADAP PERTUMBUHAN PYTHIUM SP. PENYEBAB PENYAKIT REBAH KECAMBAH MENTIMUN SECARA IN VITRO

2010 ◽  
Vol 10 (1) ◽  
pp. 59-63
Author(s):  
Subli Mujim Mujim
Keyword(s):  
Mycelial Growth ◽  
Fungal Growth ◽  
Zingiber Officinale ◽  
Spore Production ◽  
Damping Off ◽  
Ginger Extract ◽  
Randomized Design ◽  
Ginger Rhizome ◽  
Completely Randomized Design

Effect of  the  ginger extract (Zingiber officinale Rosc.) on the growth of Pythium sp., the causal agent of cucumber damping off disease.  The Pythium sp. often damaged cucumber seedling in seedbed.  The objective of this study was to evaluate the effectiveness of ginger extract on the growth and spore production of Pythium sp.   The study was conducted in 2005 at the laboratory of plant pathology, University of Lampung.  The treatments were arranged in a completely randomized design with four replicates.  The treatments were consisted of five level of concentrations of ginger rhizome extract i.e, 0; 2.5; 5.0; 7.5 and 10 %.    The results show that all concentrations of the rhizome ginger extract inhibited mycelial growth and spore production of Pythium sp.  There was a significant linear relationship between the rhizome  ginger extract and both of fungal growth and spore production.

scholarly journals In vitro antimicrobial activity and alternative control of anthracnose in papaya

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Héber Ferreira dos Reis ◽  
Lilian Maria Arruda Bacchi ◽  
Silvana de Paula Quintão Scalon ◽  
Jasna Karoliny Pereira Flores
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Syzygium Aromaticum ◽  
Alternative Control ◽  
Ginger Extract ◽  
Cinnamomum Zeylanicum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
And Control

ABSTRACT: We evaluated the efficacy of natural products in the control of papaya anthracnose, in vitro and in vivo. The in vitro experiments for evaluation of mycelial growth used a completely randomized 10 × 4 factorial design (treatments × evaluation periods) with eight replicates, with sporulation evaluated at the end of the experiment. The treatments involved the use of aqueous extract at concentrations of 5 and 15% for Syzygium aromaticum (L.) Merr. & Perry (clove), Cinnamomum zeylanicum Breym (cinnamon), and Zingiber officinalis Rox. (ginger); 1 and 3% chitosan; the fungicide Prochloraz at 100 µg.mL-1; and a control (no treatment). For evaluating conidia germination, we used six treatments with five replicates. The treatments included 7.5% of each extract (clove, cinnamon, and ginger), 1.5% chitosan, and 50 µg.mL-1 of Prochloraz. For the in vivo experiment, “Formosa” papaya “Tainung 1” was used in a completely randomized design, with six treatments and four replicates to evaluate the severity of anthracnose caused by Colletotrichum gloeosporioides. The fruits were treated by immersion for 5 min with 15% clove, cinnamon, and ginger extracts, 8% chitosan, and control with distilled water, and immersion for 2 min in Prochloraz (33.75 g a.i./100 L). The treatments with 15% clove extract and 8% chitosan were effective in all evaluations, resulting in a viable alternative to the fungicide Prochloraz. The treatments with ginger extract were less effective and those with cinnamon offered intermediate control.

scholarly journals In vitro fungitoxic potential of Lippia gracilis (Schauer) essential oil against phytopathogens

2020 ◽  
pp. 667-674
Author(s):  
Kevison Romulo da Silva França ◽  
Flavia Mota de Figueredo Alves ◽  
Tiago Silva Lima ◽  
Alda Leaby dos Santos Xavier ◽  
Plínio Tércio Medeiros de Azevedo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Fungal Species ◽  
Phytopathogenic Fungi ◽  
Negative Control ◽  
Growth Speed ◽  
Randomized Design

This study evaluates the in vitro effects of Lippia gracilis essential oil on the mycelial growth of phytopathogenic fungi. Experiments were carried out using a completely randomized design to assess the effects of eight treatments. Five replicates were evaluated for each experimental group. The essential oil was incorporated into the potato dextrose culture medium and poured into Petri dishes. Treatments were comprised of different concentrations of the oil (0.0125, 0.025, 0.05, 0.1, and 0.2%), a negative control (0.0%), and two positive controls (commercial fungicides). The plates were inoculated with fungi including Colletotrichum gloeosporioides, C. musae, C. fructicola, C. asianum, Alternaria alternata, A. brassicicola, Fusarium solani, F. oxysporum f. sp. cubense, and Lasiodiplodia theobromae and were incubated for seven days at 27 ± 2°C. The following variables were measured to verify the differences observed among treatments: percentage of mycelial growth inhibition and index of mycelial growth speed. All concentrations of L. gracilis oil inhibited the mycelial growth of the fungal species evaluated. The complete inhibition was observed between concentrations of 0.0125 and 0.1%. Treatment with oil inhibited fungal growth with similar, or even greater, efficiency than commercial fungicides.. We recommend the development of in vivo tests to verify whether L. gracilis essential oil can protect against fungal disease in live plants.

scholarly journals Sensitivity of Colletotrichum gloeosporioides Isolates from Diseased Avocado Fruits to Selected Fungicides in Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Stanley Kirugo Kimaru ◽  
E. Monda ◽  
R. C. Cheruiyot ◽  
J. Mbaka ◽  
A. Alakonya
Keyword(s):  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Field Tests ◽  
Inhibitory Effect ◽  
Copper Oxychloride ◽  
Randomized Design ◽  
Agroecological Zones ◽  
Completely Randomized Design ◽  
Growing Region

Colletotrichum gloeosporioides is a serious postharvest pathogen of avocado fruits worldwide. Kenya lacks any registered fungicides for the management of the disease. Nevertheless, farmers commonly use commercially available fungicides such as Bayleton 25WP (Triadimefon 250 g/Kg), Milraz 76WP (Propineb 70% and Cymoxanil 6%), and Copper oxychloride 500WP for disease management. The efficacy of these fungicides against C. gloeosporioides is not known. The purpose of this study was therefore to test the inhibitory effect of these fungicides against 46 C. gloeosporioides isolates from avocado fruits collected from varieties grown at different agroecological zones in Murang’a County, a popular avocado-growing region in Kenya. Mycelial growth rate and sporulation for each isolate were measured in vitro on PDA plates amended with different concentrations of the fungicides. Plates were arranged in a completely randomized design with three replications per treatment. All fungicides were effective in vitro but there were significant differences in sensitivity among isolates. Bayleton had the highest mycelial inhibition followed by Milraz, while copper oxychloride had the lowest mycelial inhibition rates, ranging from 81% to 88%. However, copper oxychloride was more effective in inhibiting sporulation. The inhibitory effect of each fungicide was concentration-dependent, where twice the recommended concentration had the highest inhibitory effect, followed by the recommended concentration. Our results show that the fungicides used by farmers against C. gloeosporioides, the causal agent for anthracnose, are effective. We, however, recommend further field tests in different avocado-growing areas so as to validate their efficacy against various isolates and under different environments.

scholarly journals Responses of pubertal rabbits to dietary supplementation of ginger (Zingiber officinale rosc) rhizome powder

2021 ◽  
Vol 41 (2) ◽  
pp. 53-60
Author(s):  
I. P. Ogbuewu ◽  
T. C. Iwuji ◽  
I. F. Etuk ◽  
C. T. Ezeokeke ◽  
I. C. Okoli ◽  
...  
Keyword(s):  
Crude Protein ◽  
Zingiber Officinale ◽  
Reproductive Organ ◽  
Serum Progesterone ◽  
Treatment Groups ◽  
Randomized Design ◽  
Ginger Rhizome ◽  
Guinea Grass ◽  
Completely Randomized Design ◽  
Dietary Treatments

Seventy two (72) sexed crossbred rabbits aged 6 months were used to determine the effect of ginger rhizome powder (GRP) supplementation on reproductive organ and serum sex hormonal characteristics of pubertal rabbits. The experimental rabbits were divided into 2 treatment groups comprising 36 males and 36 females. Each group was divided into 4 dietary treatments (BT1, BT2, BT3 and BT4 ) of 9 animals and replicated thrice with 3 animals per replicate in a completely randomized design (CRD). The animals were fed formulated diet containing 2900 Kcal ME kg-1 and 18% crude protein supplemented with GRP at the rate of 0, 5, 10 and 15 g kg-1 feed respectively. Rabbits in each dietary group were fed 50gms of Guinea grass leaf meal containing 27.6% CF (dry matter basis) occasionally as additional fibre sources. Feed and water were offered ad libitum and the study lasted for 10 weeks. Testosterone values of BT (5.83 ng mL-1) and BT (6.28 ng mL-1) and follicle stimulating hormone (FSH) levels of BT (1.15 mIU mL-1) were significantly influenced (p<0.05) by ginger rhizome powder. Bucks on BT3 and BT4 had lower luteinizing hormone (LH) level than the BT1 bucks. For the females, FSH and LH values ranged significantly (p<0.05) from 0.90 to 2.05 mIU mL-1 and 0.90 to 3.15 mIU mL-1 respectively. The 17â - estradiol and progesterone levels also ranged significantly (p<0.05) from 166.35 to 175.05 pg mL-1 and 15.45 to 16.80 ng mL-1 respectively. Ovarian weight of rabbit on BT was significantly (p<0.05) higher than those on BT1 but similar to those on BT2 and BT4 treatments. It was therefore concluded that the use of ginger rhizome powder in rabbit diets improved the weight of the ovary as well as enhances serum progesterone, estrogen and testosterone levels.

scholarly journals Efektivitas Limbah Cengkih dalam Menekan Perkembangan In Vitro Sclerotium rolfsii, Jamur Penyebab Damping Off Kacang Tanah

2021 ◽  
Vol 17 (1) ◽  
pp. 36-42
Author(s):  
Handry R D Amanupunyo ◽  
Nace E Tahitu ◽  
Gratiana N C Tuhumury
Keyword(s):  
Sclerotium Rolfsii ◽  
Plant Diseases ◽  
Damping Off ◽  
Flower Stalk ◽  
Colony Diameter ◽  
Randomized Design ◽  
Leaf Powder ◽  
Peanut Production ◽  
Completely Randomized Design

Various problems have caused the decline in peanut production, among others the low resistance of peanut plants to various diseases. One of the obstacles in peanut production is the attack of plant diseases, such as the fungus Sclerotium rolfsii Sacc. The use of plant-based insecticides can be an alternative to disease control in peanuts. This study aimed to obtain the appropriate and effective dose of clove leaves and flower stalks in suppressing the in vitro growth of the fungus S. rolfsii Sacc causing damping off in peanuts, and to test the effectiveness of clove leaves and flower stalks on the formation of sklerotia of the fungus. This study used ‘tuni’ clove powder from Waai Village, Salahutu District. This experimental study employed a completely randomized design (CRD) with 8 treatments and 3 replications. The results showed that the growth of the fungus S. rolfsii Sacc. was inhibited by clove flower stalk powder at a dose of 1.5 g per100 mL of PDA, which was indicated by the number of sklerotia formed of 13.67% and propagule weight of 0.12 g, followed by a dose of clove leaf powder of 1.5 g per100 mL PDA, with amount of sklerotia formed of 27.33% and propagule weight of 0.17 g. These results were statistically shown on the parameters: colony diameter, Sklerotia formation time, number of Sklerotia, and weight of propagules. Keywords: Clove powder, dosage, in vitro, Sclerotium rolfsii Sacc.   ABSTRAK Berbagai masalah telah menyebabkan turunnya produksi kacang tanah, diantaranya masih rendahnya ketahanan tanaman kacang tanah terhadap berbagai penyakit. Salah satu kendala dalam produksi kacang tanah adalah serangan penyakit tanaman, seperti jamur Sclerotium rolfsii Sacc. Penggunaan insektisida nabati dapat menjadi alternatif pengendalian penyakit pada kacang tanah. Penelitian ini bertujuan untuk memperoleh dosis daun dan tangkai bunga cengkih yang tepat dan efektif dalam menekan pertumbuhan jamur S. rolfsii Sacc penyebab damping off pada kacang tanah secara in vitro, serta menguji efektifitas daun dan tangkai bunga cengkih terhadap pembentukan sklerotia jamur. Penelitian ini menggunakan bubuk cengkih tuni, dari Desa Waai, Kecamatan Salahutu. Penelitian eksperimental ini menggunakan Rancangan Acak Lengkap (RAL) dengan 8 perlakuan dan 3 ulangan. Hasil penelitian menunjukkan bahwa pertumbuhan jamur S. rolfsii Sacc. terhambat oleh bubuk tangkai bunga cengkih pada dosis 1,5 g per 100 mL PDA yang ditunjukan oleh jumlah sklerotia terbentuk 13,67% dan berat propagul 0,12 g dan diikuti oleh dosis bubuk daun cengkih 1,5 g per 100 mL PDA, dengan jumlah sklerotia 27,33% dan berat propagul 0,17 g. Hasil ini secara statistik ditunjukkan pada parameter: diameter koloni, waktu terbentuk sklerotia, jumlah sklerotia, dan berat propagul. Kata kunci: bubuk cengkih, dosis, in vitro, Sclerotium rolfsii Sacc.

PENGARUH PENAMBAHAN SARI JAHE (Zingiber Officinale) TERHADAP SIFAT KIMIA DAN ORGANOLEPTIK DODOL SERAT KEDELAI

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Lasran Lasran ◽  
Hermanto Hermanto ◽  
Muhammad Syukri Sadimantara
Keyword(s):  
Chemical Analysis ◽  
Confidence Level ◽  
Zingiber Officinale ◽  
Ash Content ◽  
National Standard ◽  
Crude Fiber ◽  
Ginger Extract ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test

ABSTRACTThis study aimed to determine the effect of adding ginger juice on the organoleptic and chemical values of soy fiber dodol products. This study used a completely randomized design consisting of the addition of ginger extract (F), namely treatment F0 (0%), F1 (1%), F2 (2%), F3 (3%), and F4 (4%). Data were analyzed using Analysis of Variance and continued with Duncan's Multiple Range Test (DMRT) at a 95% confidence level (α=0.05). The addition of ginger juice had a very significant effect on the parameters of aroma, texture, color, and significantly different on taste. Based on the results of the organoleptic assessment, the most preferred treatment was F2 treatment (addition of 2% ginger juice) with color, aroma, and texture rating scores reached 3.89 (like), 3.80 (like), and 3.56 (like), respectively. The chemical analysis results show that the selected treatment had 17.53% water, 1.60% ash, 12.25% protein, 31.37% fat, 10.52% fiber, and 9.79% crude fiber. Among the five parameters, parameters of water, fat, protein, and crude fiber contents met the national standard but the ash content did not.Keywords: lunkhead, soybean dregs, gingerABSTRAKPenelitian ini bertujuan untuk menentukan pengaruh penambahan sari jahe terhadap nilai organoleptik dan nilai kimia produk dodol serat kedelai. Penelitian ini menggunakan Rancangan Acak Lengkap yang terdiri atas penambahan sari jahe (F) yaitu perlakuan F0 (0%), F1 (1%), F2 (2%), F3 (3%) dan F4 (4%). Data dianalisis menggunakan Analisis ragam (Analysis of Varian) dan dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95% (α=0,05). Penambahan sari jahe berpengaruh sangat nyata terhadap parameter aroma, tekstur, warna dan berbeda nyata pada rasa. Berdasarkan hasil penilaian organoleptik diperoleh perlakuan terpilih yaitu perlakuan F2 (penambahan sari jahe 2%) dengan skor penilaian warna 3,89 (suka) aroma 3,80 (suka), tekstur 3,56 (suka).dengan nilai analisis kimia meliputi analisis kadar air 17,53%, kadar abu 1,60%, kadar protein 12,25%, dan kadar lemak 31,37%, kadar serat 10,52%, serat kasar 9,79%. dari 5 parameter ada 4 parameter kadar air, kadar lemak, kadar protein dan kadar serat kasar telah memenuhi SNI dan 1 parameter yaitu kadar abu tidak sesuai standar SNI.Kata kunci: dodol, ampas kedelai, jahe

scholarly journals Pengaruh Uji Antibakteri Ekstrak Rimpang Jahe Terhadap Pertumbuhan Staphylococcus Aureus Dan Escherichia Coli Secara In Vitro

2020 ◽  
Vol 1 (2) ◽  
pp. 71-80
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Randomized Design ◽  
Ginger Rhizome ◽  
Completely Randomized Design

The problem of resistance is increasing along with the need for antimicrobials as an alternative in overcoming resistance problems. One solution is to utilize the content of secondary metabolite compounds in rhizome plants such as ginger. The content of compounds that are usually the most dominant in ginger such as essential oils, flavonoids, terpenoids and phenols. This study aims to determine the effect of ginger rhizome extract as an antibacterial on the growth of Staphylococcus aureus and Escherichia coli in vitro. This study was a laboratory experimental study with a completely randomized design (CRD) consisting of 7 treatments with 3 replications. The results showed that the ethanol extract of ginger rhizome had antibacterial activity against S. aureus and E. coli. At concentrations of 20%, 40% and 80% which are more effective in inhibiting the growth of these two bacteria. The higher the extract concentration level, the larger the diameter of the inhibition zone from bacterial growth. This inhibition of bacterial growth is thought to have an effect on the content of the ginger root.   Keywords: Ginger rhizome, Staphylococcus aureus, Escherichia coli, antibacterial ABSTRAK   Permasalahan tentang resistensi semakin meningkat seiring dengan kebutuhan antimikroba sebagai alternatif dalam mengatasi masalah resistensi. Salah satu solusinya yaitu dengan memanfaatkan kandungan dari senyawa metabolit sekunder pada tanaman rimpang-rimpangan seperti rimpang jahe. Kandungan senyawa yang biasanya paling dominan pada jahe seperti minyak atsiri, flavonoid, terpenoid dan fenol. Penelitian ini bertujuan untuk mengetahui pengaruh dari ekstrak rimpang jahe sebagai antbakteri terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli secara in vitro. Penelitian ini termasuk penelitian eksperimental laboratorium dengan rancangan acak lengkap (RAL) yang terdiri dari 7 perlakuan dengan 3 kali ulangan. Hasil penelitian diperoleh bahwa ekstrak etanol rimpang jahe memiliki aktivitas antibakteri terhadap S. Aureus dan E. coli. Pada konsenrasi 20%, 40% dan 80% yang lebih efktif dalam menghambat pertumbuhan kedua bakteri tersebut. Semakin tinggi tingkatan konsentrasi ekstrak maka diameter zona hambat dari pertumbuhan bakteri juga semakin besar. Terhambatnya pertumbuhan bakteri ini diduga adanya pengaruh dari kandungan pada rimpang jahe.   Kata Kunci: Rimpang Jahe, Staphylococcus aureus, Escherichia coli, antibakteri

scholarly journals Fungitoxicity of the Pyroligneous Extract in the Development in vitro of Colletotrichum gloeosporioides

2020 ◽  
Vol 8 (4) ◽  
pp. 82
Author(s):  
Cleverson Rodrigues ◽  
João Aguilar Massaroto ◽  
Grace Queiroz David ◽  
Dilânia Lopes de Matos ◽  
Walmor Moya Peres ◽  
...  
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Tectona Grandis ◽  
Growth Speed ◽  
Randomized Design ◽  
Agar Media ◽  
Completely Randomized Design ◽  
Development In Vitro

Products derived from renewable sources have been studied for their use in agriculture enabling increments in the production of agricultural culture, such as in the case of the pyroligneous extract. The research aimed at evaluating the fungitoxic potential of the pyroligneous extract of teak (Tectona grandis L.) on mycelial growth, sporulation and spore germination of Colletotrichum gloeosporioides. The experiment was conducted in completely randomized design with five treatments and four replications. Five doses of pyroligneous extract were tested (0, 25, 50, 100, 150 mL L-1) following the pour-plate methodology in 10 mL of potato dextrose agar media and kept in the incubating room maintained at 25 ± 2 °C in a 12 hour-photoperiod for eight days. Mycelial growth variables (cm), mycelial index growth speed, growth inhibition (%), sporulation inhibition (%) and spore germination (%) were evaluated. There was a reduction for the mycelial growth as well as for the rate of mycelial growth, sporulation inhibition and spore germination as the doses were increased. The percentage inhibition of mycelial growth was 56 % for the highest dose (150 mL L-1). Pyroligneous extract of teak has a direct fungitoxic action on growth, sporulation and germination in vitro of the fungus C. gloeosporioides.

scholarly journals In-vitro evaluation of botanicals and fungicides against Bipolaris sorokiniana, causing spot blotch of wheat

2020 ◽  
Vol 3 (2) ◽  
pp. 296-305
Author(s):  
Prem Bahadur Magar ◽  
Suraj Baidya ◽  
Rabina Koju ◽  
Sagar Adhikary
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
In Vitro Experiment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Poison Food Technique ◽  
Concentration Levels

Spot blotch disease of wheat caused by Bipolaris sorokiniana; is one of the most concerning disease in warm and humid regions of Nepal due to its widespread prevalence and increasing severity. An in-vitro experiment was conducted to evaluate the efficacy of different botanicals and fungicides against Bipolaris sorokiniana, in National Plant Pathology Research Centre’s laboratory at Khumaltar, Lalitpur in 2019. The experiment was done in completely randomized design with three replications by poison food technique. Five botanicals viz. neem, garlic, eucalyptus, bojho and asuro at three different concentrations (5%, 10% and 15%) and eight fungicides viz. sectin, curex, bavistin, vacomil plus, saaf, sajha, criptan and tilt at three concentration levels (25 ppm, 50 ppm and 100 ppm) except curex (50 ppm, 100 ppm and 200 ppm) were used to evaluate the effect on the radial mycelial growth and mycelial growth inhibition percentage of the pathogen. The different botanical extracts and fungicides in different concentrations inhibited the mycelial growth of fungus significantly (p<0.01). The highest mycelial growth inhibition percentage was found by the application of garlic clove extract (52.85%) at 15% which was followed by bojho (52.48%) at 15% concentration. While tilt was found effective fungicide which completely inhibits the mycelial growth at all concentrations. Also, fungicides viz. sajha (79.78%), saaf (73.59%) and sectin (70%), at 100 ppm were found effective in controlling the pathogen as compare to others. Mycelial growth inhibition of the fungus was found to be increased with the increase in concentrations of botanicals and fungicides.

scholarly journals EFIKASI SUHU PROSES PRODUKSI ASAP CAIR DAN KONSENTRASI ASAP CAIR DARI KAYU LABAN (VITEX PUBESCENS) TERHADAP JAMUR OPHIOSTOMA PILIFERUM

Agrin ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 49
Author(s):  
Hasan Oramahi ◽  
Farah Diba ◽  
Nurhaida Nurhaida ◽  
Wahdina Wahdina ◽  
Dina Setyawati ◽  
...  
Keyword(s):  
Pyrolysis Temperature ◽  
Fungal Growth ◽  
Petri Dish ◽  
Potato Dextrose Agar ◽  
Test Results ◽  
Wood Vinegar ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Ophiostoma Piliferum

Efficacy wood vinegar produced from wood laban (Vitex pubescens) against Ophiostoma piliferum fungus was evaluated. The objectives of this research to perform in vitro antifungal of wood vinegar from laban wood against O. piliferum. The source of lignocelluloses biomass was carbonization process used three temperature i.e. 350, 400 and 450 °C.  Efficacy of wood vinegar to the fungus carried on PDA (potato dextrose agar) in a Petri dish that has been mixed with the wood vinegar with a concentration of 0, 0.5, 1.0, 1.5, and 2.0 % (v/v). Antifungal test was a factorial 3 by 5 in a completely randomized design. The means were seperated using using Tukey’s test at p = 0.05. All data were analyzed using the SAS softwere (version 8.2, SAS Institute Inc., NC. USA). The test results showed that the pyrolysis suhue effect and concentration of wood vinegar on the inhibition of fungal growth. The higher pyrolysis temperature and concentration the inhibition of fungal growth was increased.Efikasi asap cair dari kayu laban (Vitex pubescens)terhadap Ophiostoma piliferum telah dilakukan.Penelitian ini bertujuan mengevaluasi kemampuan asap cair sebagai antijamur (O. piliferum) secara in vitro. Asap cair yang digunakan diperoleh dengan cara pirolisis pada suhu produksi asap cair dan konsentrasi asap cair yang berbeda. Efikasi asap cair terhadap jamur dilakukan pada media PDA (potato dextrose agar) dalam cawan Petri dengan campuran asap cair pada konsentrasi 0, 0,5, 1,5, dan 2,0 (v/v).Pengujian aktivitas antijamur dilakukan menggunakan rancangan acak lengkap tipe faktorial.Data yang diperoleh dianalisis dengan sidik ragam.Pengaruh  antarperlakuan dilakukan uji BNJ pada taraf nyata 5%. Analisis data menggunakan SAS versi 9.13. Hasil pengujian menunjukkan bahwa suhu pirolisis produksi asap cair dan konsentrasi asap cair berpengaruh terhadap daya penghambatan pertumbuhan jamur. Makin tinggi suhu pirolisis produksi asap cair dan konsentrasinya dan makin tinggi daya penghambatan terhadap pertumbuhan jamur, O. piliferum.

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