scholarly journals Hydrolysis of αs2-casein in solution by chymosin, plasmin, trypsin and Lactobacillus-proteinases

1993 ◽  
Vol 2 (2) ◽  
pp. 133-139
Author(s):  
Anne Pihlanto-Leppälä ◽  
Eero Pahkala ◽  
Minna Kahala ◽  
Veijo Antila

The aim of this study was to examine the enzymic hydrolysis of αs2-casein by isolating and identifying the released peptides. The enzymes applied in the study were chymosin, plasmin and trypsin as well as cell free extracts from three strains of Lactobacillus helveticus and nine strains of L. casei. The findings showed that chymosin had weak proteolytic activity on αs2-casein. Plasmin, on the other hand, released numerous peptides under the used conditions. The majority of the identified fragments were released from the C terminal end of the substrate. Plasmin hydrolysed mainly Lys-X bonds. The third enzyme, trypsin, hydrolysed several bonds of αs2-casein. Peptides were released from almost all regions of the protein. Trypsin acted on the carboxyl sides of arginyl and lysyl residues. Cell free extract of lactobacilli had little activity on αs2-casein.

1993 ◽  
Vol 2 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Anne Pihlanto-Leppälä ◽  
Eero Pahkala ◽  
Veijo Antila

The aim of this study was to examine the enzymatic hydrolysis of κ-casein by isolating and identifying the released peptides. The enzymes employed in the study were chymosin, plasmin and trypsin, as well as a cell-free extract from three Lactobacillus helveticus and nine Lactobacillus casei strains. The findings showed that the bond most sensitive to the proteolytic activity of chymosin was the Phe 105-Met 106. After 24 hours of hydrolysis a few other bonds in the casein macropeptide were also cleaved. Plasmin was found to have weak proteolytic activity under the conditions of this study. When the enzyme-substrate ratio was raised from 1:200 to 1:50, a few peptides were released from the N-terminal region. Trypsin was found to hydrolyze several κ-casein bonds, and peptides were released from almost all regions of the protein. The proteases of Lactobacillus had less effect than chymosin, plasmin or trypsin. The strains could be divided into three categories. L. helveticus strains had activity on bonds in the mid-section and C-terminal region, L. casei strains EB, P3, P8 and A 1 had activity on bonds in the N- and C-terminal regions, while L. casei A5 and M9 had activity only on bonds in the C-terminal region.


2020 ◽  
pp. 13-61
Author(s):  
Natalia Małecka-Drozd

The 3rd millennium BC appears to be a key period of development of the historical settlement landscape in ancient Egypt. After the unification of the country, the process of disappearance of the predynastic socio-political structures and settlement patterns associated with them significantly accelerated. Old chiefdoms, along with their centres and elites, declined and vanished. On the other hand, new settlements emerging in various parts of the country were often strictly related to the central authorities and formation of the new territorial administration. Not negligible were climatic changes, which influenced the shifting of the ecumene. Although these changes were evolutionary in their nature, some important stages may be recognized. According to data obtained during surveys and excavations, there are a number of sites that were considerably impoverished and/or abandoned before and at the beginning of the Old Kingdom. On the other hand, during the Third and Fourth Dynasties some important Egyptian settlements have emerged in the sources and begun their prosperity. Architectural remains as well as written sources indicate the growing interest of the state in the hierarchy of landscape elements and territorial structure of the country.


Vox Patrum ◽  
2011 ◽  
Vol 56 ◽  
pp. 339-348
Author(s):  
Bogdan Czyżewski

Although St. Basil did not live 50 years, the topic of the old age appears in his works quite often. On the other hand, it is clear that Basil does not discuss this issue in one par­ticular work or in the longer argumentation. The fragmentary statements about old age can be found in almost all his works, but most of them can be found in the correspondence of Basil. In this paper we present the most important ad the most interesting aspect of teach­ing of Basil the Great. As these certificates show that the bishop of Caesarea looked at the old age maturely, rationally estimated passage of time, which very often makes a man different. He experienced it, for example as a spiritual and physical suffering, which often were connected with his person. He saw a lot of aspect of the old age, especially its advan­tages – spiritual maturity and wisdom. What is more, he pointed also to passage of time, which leads a man to eternity, which should be prepared to, regardless how old he is. In his opinion fear is not seen opinions of St. Basil present really Christian way of thinking, well-balanced and calm.


Zootaxa ◽  
2021 ◽  
Vol 5051 (1) ◽  
pp. 346-386
Author(s):  
SÜPHAN KARAYTUĞ ◽  
SERDAR SAK ◽  
ALP ALPER ◽  
SERDAR SÖNMEZ

An attempt was made to test if Lourinia armata (Claus, 1866)—as it is currently diagnosed—represents a species complex. Detailed examination and comparisons of several specimens collected from different localities suggest that L. armata indeed represents a complex of four closely related morphospecies that can be differentiated from one another by only detailed observations. One of the four species is identified as Lourinia aff. armata and the other three species are described as new to science and named as Lourinia wellsi sp. nov., L. gocmeni sp. nov., and L. aldabraensis sp. nov. Detailed review of previous species records indicates that the genus Lourinia Wilson, 1924 is distributed worldwide. Ceyloniella nicobarica Sewell, 1940, originally described from Nicobar Island and previously considered a junior subjective synonym of L. armata is reinstated as Lourinia nicobarica (Sewell, 1940) comb. nov. on the basis of the unique paddle-shaped caudal ramus seta V. It is postulated that almost all of these records are unreliable in terms of representing true Lourinia aff. armata described herein. On the other hand, the comparative evaluation of the illustrations and descriptions in the published literature indicates the presence of several new species waiting to be discovered in the genus Lourinia.                 It has been determined that, according to updated modern keys, the recent inclusion of the monotypic genus Archeolourinia Corgosinho & Schizas, 2013 in the Louriniidae is not justified since Archeolourinia shermani Corgosinho & Schizas, 2013 does not belong to this family but should be assigned to the Canthocamptidae. On the other hand, it has been argued that the exact phylogenetic position of the Louriniidae still remains problematic since none of the diagnostic characters supports the monophyly of the family within the Oligoarthra. It has also been argued that the close relationship between Louriniidae and Canthocamptidae is supported since both families share the homologous sexual dimorphism (apophysis) on P3 endopod. The most important characteristic that can possibly be used to define Louriniidae is the reduction of maxilliped.  


De Jure ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Daniel Haman ◽  
◽  
◽  

The difference between intent (dolus) and negligence (culpa) was rarely emphasized in codified medieval laws and regulations. When compared to the legal statements related to intent, negligence was mentioned even more rarely. However, there are some laws that distinguished between the two concepts in terms of some specific crimes, such as arson. This paper draws attention to three medieval Slavic legal documents – the Zakon Sudnyj LJudem (ZSLJ), the Vinodol Law and the Statute of Senj. They are compared with reference to regulations regarding arson, with the focus being on arson as a crime committed intentionally or out of negligence. The ZSLJ as the oldest known Slavic law in the world shows some similarities with other medieval Slavic legal codes, especially in the field of criminal law, since most of the ZSLJ’s articles are related to criminal law. On the other hand, the Vinodol Law is the oldest preserved Croatian law and it is among the oldest Slavic codes in the world. It was written in 1288 in the Croatian Glagolitic script and in the Croatian Chakavian dialect. The third document – the Statute of Senj – regulated legal matters in the Croatian littoral town of Senj. It was written in 1388 – exactly a century after the Vinodol Law was proclaimed. When comparing the Vinodol Law and the Statute of Senj with the Zakon Sudnyj LJudem, there are clear differences and similarities, particularly in the field of criminal law. Within the framework of criminal offenses, the act of arson is important for making a distinction between intent and negligence. While the ZSLJ regulates different levels of guilt, the Vinodol Law makes no difference between dolus and culpa. On the other hand, the Statute of Senj strictly refers to negligence as a punishable crime. Even though the ZSLJ is almost half a millennium older than the Statute of Senj and around 400 years older than the Vinodol Law, this paper proves that the ZSLJ defines the guilt and the punishment for arson much better than the other two laws.


2019 ◽  
Vol 2 (3) ◽  
pp. 319
Author(s):  
Titi Mariah ◽  
Yanti Yusanti ◽  
Ula Nisa El Fauziah

Vocabulary known as an essential skill for learning to read, speak, write and listen. It is important to find the appropriate technique in order to improve students’ vocabulary mastery. The researchers did research with focuses on students’ perception towards using “dictionary pocket” in order to improve their vocabulary mastery. It was conducted using a qualitative descriptive method. To collect the data, the researchers use the instruments of an open-ended questionnaire and interview section. The samples were 36 students of second grade from Broadcasting major at SMK Negeri 1 Cimahi. The result of the questionnaire shows that it was found that almost all of the students feel that with learn vocabulary can be easier when they use the technique of remembering dictionary pocket. They also agree that dictionary pocket is an appropriate technique which is used to learn vocabulary. In the other hand, the result of interview shows, the students concede that they are feel helped and being motivated. It can be sum up that dictionary pocket is suitable to strengthen their vocabulary mastery. Dictionary pocket is useful enough because it can help them when they forget the word.


2021 ◽  
Vol 22 (1) ◽  
pp. 131-142
Author(s):  
Mohammad Ebrahim Ahmed ◽  
Hussain Yawr Hussain

Thirty-six local dose have been used and were divided in to three groups sacording to weight, in each group twelve dose. In the first group the weights of the dose were higher than ( 2.5 kgs) and less than (3 kgs). The second groups was higher than 3 kgs) and less than (3.5 kgs). The third group was higher than (3.5 kgs) and less than (4 kgs). The aim of this study was to obtain the effect of doe weight on her reproductive efficiency (gestation period, litter size, litter weight at birth and weaning, growth rate of offspring preweaning, conception rate and preweaning mortility).  The weight of the doe had no significant effect upon the gestation period and this period was 30.9, 31.2, 31.3 days for the three groups respectively, on the other hand the litter size was affected significantly by doe weight and litter size was at birth and preaweaning (5.1 , 5.8 and 6.2), (4.5, 5.3 and 5.7) for the three groups respectively. The offspring weight at birth and weaning was affected significantly by doe weight and this weight was (40.1 , 48.2 and 53.3 gms), (203.6, 227.5 and 233.8 gms) for the three groups respectively while the conception rate was not affected by doe weight. The mortility percentage in the prewaning period was not significantly by doe weight


2017 ◽  
Vol 56 (1-4) ◽  
Author(s):  
Anikó Polgár

This study is dealing with two Hungarian translations of Euripides’ Medea. The translation made by Grácia Kerényi was produced in the second half of the 20th century, whereas the version by Zsuzsa Rakovszky was published at the beginning of the 21st. The difference between the translations regarding their textual strategies, the professional background of the translators and the final goal of the works is abysmal. Grácia Kerényi was an expert of ancient literatures, her translation was published in the official and renowned collection of Euripides’ work, Zsuzsa Rakovszky on the other hand translates predominantly from English, and her version was inspired by the request of the theatre. The study contains three parts: in the first the author analyses Kerényi’s Medea in the context of the philological reconstruction, in the second, the author examines the same text modified and revised by Fruzsina Magyar, who was the dramatic advisor of the theatre performance in Szolnok, and the third part reflects on the problems of validity, poetical force and immediacy in the translation of Zsuzsa Rakovszky.


2019 ◽  
Vol 21 (1) ◽  
pp. 64-86
Author(s):  
Netanel Nissim ◽  
Aner Sela

We study an elimination tournament with four contestants, each of whom has either a high value of winning (a strong player) or a low value of winning (a weak player) and these values are common knowledge. Each pairwise match is modeled as an all-pay auction. The winners of the first stage (semifinal) compete in the second stage (final) for the first prize, while the losers of the first stage compete for the third prize. We examine whether or not the game for the third prize is profitable for the designer who wishes to maximize the total effort of the players. We demonstrate that if the players are asymmetric and there are at least two strong players, then there is always a seeding of the players such that the third place game is not profitable. On the other hand, if there are at least two weak players, then there is always a seeding of the players such that the third place game is profitable.


1997 ◽  
Vol 39 (3) ◽  
pp. 159-164 ◽  
Author(s):  
Christiane Finardi PANCERA ◽  
Adriana Leal ALVES ◽  
Maria Aparecida PASCHOALOTTI ◽  
Pedro Paulo CHIEFFI

Mebendazole, albendazole, levamisole and thiabendazole are well known as active drugs against several nematode species, and against cestodes as well, when the first two drugs are considered. None of the drugs have proven activity, however, against trematodes. We tested the effect of these drugs on the fecal shedding of schistosome eggs and the recovering of adult schistosomes, after portal perfusion in Schistosoma mansoni experimentally infected mice. Balb/c mice infected with 80 S. mansoni cercariae were divided into three groups, each in turn subdivided into four other groups, for each tested drug. The first group was treated with each one of the studied drugs 25 days after S. mansoni infection; the second group was submitted to treatment with each one of the drugs 60 days after infection. Finally, the third group, considered as control, received no treatment. No effect upon fecal shedding of S. mansoni eggs and recovering of schistosomes after portal perfusion was observed when mice were treated with either mebendazole or albendazole. Mice treated with either levamisole or thiabendazole, on the other hand, showed a significant reduction in the recovering of adult schistosomes after portal perfusion, mainly when both drugs were given during the schistosomula evolution period, i.e., 25 days after cercariae penetration, probably due to unspecific immunomodulation


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