scholarly journals PERBANDINGAN KOMPONEN MINYAK ATSIRI ANTARA DAUN MUDA DAN DAUN DEWASA PADA HYPTIS SUAVEOLENS (L.)POIT

2017 ◽  
Vol 18 (02) ◽  
pp. 1-12
Author(s):  
Moralita Chatri ◽  
Mansyurdin Mansyurdin ◽  
Amri Bakhtiar ◽  
Perri Adnadi

Hyptis suaveolens (L.) Poit is one of the species Lamiaceae family (Labiatae), is an aromatic plant and can produce essential oils. Essential oils of these plants can be utilized as antimicrobials. The chemical components contained in the essential oils in the same plant may vary as they are influenced by several factors, including the growth stage. Essential oils can be obtained by hydrodestilation and their components are analyzed using gas chromatography-mass spectrometry (GC-MS). The essential oil components are then compared between those found in young leaves and adult leaves. The results of analysis and identification showed that in young leaves there are 50 components while in adult leaves there are only 37 components. The main components of young leaves were ?-caryophyllene (34.65%), germacrene-D (10.32%), ?-bergamotene (6.56%), ?-copaene (5.94%) and rimuene (6.46%), Are ?-caryophyllene (23.53%), germacrene-D (9.52%), ?-elemente (6.53%) and rimuene (13.19%). The components of ?-bergamotene and ?-copaene are not found in adult leaves.

2004 ◽  
Vol 1 (3) ◽  
pp. 301-303 ◽  
Author(s):  
Betül Demirci ◽  
Dietrich H. Paper ◽  
Fatih Demirci ◽  
K. Hüsnü Can Başer ◽  
Gerhard Franz

The essential oil ofBetula pendulaRoth. buds was obtained using both hydrodistillation and microdistillation techniques and their chemical compositions were analyzed using both gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS). Overall, more than 50 compounds were identified representing 80% and 92% for hydrodistillation and microdistillation, respectively. The main components (by hydrodistillation and microdistillation, respectively) found were α-copaene (12% and 10%), germacrene D (11% and 18%) and δ-cadinene (11% and 15%) in the analyzed essential oils. The microdistillation technique proved to be a useful tool and compliant alternative when compared to hydrodistillation.


2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Sarin Tadtong ◽  
Narisa Kamkaen ◽  
Rith Watthanachaiyingcharoen ◽  
Nijsiri Ruangrungsi

This study focused on characterization of the chemical components of an aromatherapy recipe. The formulation consisted of four blended essential oils; rosemary oil, eucalyptus oil, pine oil and lime oil (volume ratio 6: 2: 1: 1). The single and combination essential oils were identified by gas chromatography-mass spectrometry (GC-MS). The analysis of GC-MS data revealed that several components exist in the mixture. The five most important components of the blended essential oils were 1,8-cineole (35.6 %), α-pinene (11.1 %), limonene (9.6 %), camphor (8.4 %), and camphene (6.6 %). The main components of rosemary oil were 1,8-cineole (37.3 %), α-pinene (19.3 %), camphor (14.7 %), camphene (8.8 %), and β-pinene (5.5 %); of eucalyptus oil 1,8-cineole (82.6 %) followed by limonene (7.4 %), o-cymene (4.3 %), γ-terpinene (2.7 %), and α-pinene (1.5 %); of pine oil terpinolene (26.7 %), α-terpineol (20.50 %), 1-terpineol (10.8 %), α-pinene (6.0 %), and γ-terpineol (5.3 %); and of lime oil limonene (62.9 %), γ-terpinene (11.5 %), α-terpineol (7.6 %), terpinolene (6.0 %), and α-terpinene (2.8 %). The present study provided a theoretical basis for the potential application of blended essential oils to be used as an aromatherapy essential oil recipe. GC-MS serves as a suitable and reliable method for the quality control of the chemical markers.


2021 ◽  
Vol 10 (3) ◽  
pp. 191-195

In this study, the antitrypanosomal action of Lavandula angustifolia Mill. (Lavender), Matricaria chamomilla L. (chamomile) and Ocimum basilicum L. (Basil) essential oils was investigated against Trypanosoma evansi. The Gas Chromatography/Mass Spectrometry (GC/MS) was used to analyze these essential oils and identifying their chemical components. The main components present in lavender oil were 1,8-cineole (88.02%) and camphor (8.09%). In chamomile oil, bisabolol oxide (43.81%), cis-α-farnesene (17.79%) and bisabolone oxide (7.71%) were found to be the most predominant components. Basil oil is principally composed of benzene, 1-methoxy-4-(2-propenyl) (52.68%), L-linalool (29.46%) and 1, 8-cineole (9.46%). In vitro results revealed that all the tested oils exhibited notable trypanocidal action and inhibited the growth of T. evansi. The minimum antitrypanosomal activity was determined by chamomile oil and the strongest was determined by lavender oil. These findings suggest that the tested essential oils could be investigated in future studies for the treatment of T. evansi.


HortScience ◽  
2017 ◽  
Vol 52 (6) ◽  
pp. 827-830 ◽  
Author(s):  
Mostafa Farajpour ◽  
Mohsen Ebrahimi ◽  
Amin Baghizadeh ◽  
Mostafa Aalifar

Chemical composition and essential oil yields from aerial parts of 31 Iranian Achillea millefolium accessions, each collected from their natural habitats of Iran and grown together in field conditions, were investigated. The concentrations of the hydro-distilled essential oils ranged from 0.03% to 0.39%. Gas chromatography–mass spectrometry (GC–MS) analysis revealed 50 compounds in the accessions. The main components of the essential oils in Iranian A. millefolium accessions varied in the following ranges: 1,8-Cineole, 1.2–19.8%; β-thujone, 0.4–55.3%; camphor, 0.6–25.5%; germacrene-D, 2–20.6%; trans-nerolidol, 0.4–48.1%; isospathulenol, 0.5–36%; and cubenol, 0.1–42.9%. According to cluster analysis, five chemotypes were obtained as 1,8-Cineole/trans-nerolidol, high cubenol, high germacrene-D/isospathulenol, high camphor/cubenol, and high 1,8-Cineole/ β-thujone/cubenol. The result of principal component analysis (PCA) indicated that germacrene-D and isospathulenol components were under more genetic control than the other main components. Results revealed a high level of variation of composition and yield of essential oils among the Iranian A. millefolium accessions.


2016 ◽  
Vol 16 ◽  
pp. 34-38 ◽  
Author(s):  
N. Javzmaa ◽  
Sh. Altantsetseg ◽  
S. Shatar ◽  
T. Enkhjargal ◽  
Z. Anu

The essential oil compositions of four Artemisia species in Mongolian Trans-Altai Gobi were studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The oil from A.macrocephala Jacq and A.dracunculus Ledeb. were characterized by the presence of monoterpene hydrocarbons and oxygenated monoterpeneoids predominately. E-nerolidol (26.95%), methyleugenol (23.29%) and sabinene (13.21%) were found as main components in the essential oils of A.dracunculus. A.macrocephalla was characterized by the presence of chamazulene (13.8%), cineol (11.7%), myrcene (9.0%), germacrene-D (7.1%). A.anethifolia Web was characterized by the presence of fragrant compounds as camphor (26.05%), α-thujone (10.1%), borneol (5.1%). Davanone and davanone derivatives were also detected in the sample in amount of 7.7% in total. A.scoparia Waldst differed by domination of monoterpene hydrocarbons (78.9%) with (Z)- β-ocimene (29.24%), α-pinene (15.19%), limonene (10.27%) and myrcene (9.61%).Mongolian Journal of Chemistry 16 (42), 2015, 34-38


2020 ◽  
Vol 10 ◽  
Author(s):  
Ionara I. Dalcol ◽  
Alessandra O. Pereira ◽  
Luisa H. Paz ◽  
Gabriela Benetti ◽  
Fallon S. Siqueira ◽  
...  

Background: Aristolochia triangularis Cham., popularly known as the "cipó-mil-homens", "angelicó" and "ypê-mi", is applied for the treatment of wounds, skin diseases (6,7), digestive and circulatory system diseases as an antipyretic and for malaria fever. Objective: In this work we investigated the chemical composition, the antimicrobial and antimycobacterial activities of the essential oils (EOs) extracted from A. triangularis fresh stems and leaves collected in Southern Brazil. Methods: Fresh stems and fresh leaves of Aristolochia triangularis Cham. were separately subjected to hydrodistillation using a Clevenger-type apparatus. The chemical composition of the essential oils (EOs) were analyzed by gas chromatography/mass spectrometry (GC/MS). The oil samples were evaluated for their antimycobacterial, antibacterial and antifungal activities against twenty-four microorganisms. Results: Hydrodistillation of fresh stems and leaves of A. triangularis resulted in 0.16% (w/w) and 0.37% (w/w) respectively of light-yellow oils. Germacrene D is found in 13.2 - 13.5% in both EOs. The constituent most abundant in the stems EO (19.18%) was the oxygenated diterpene ent-Kaur-16-en-19-al (10), along with E-nerolidol (17.89%). The main constituents of the leaves EO were bicyclogermacrene (24.79%), β-elemene (11.30%), E-caryophyllene (10.40%) and germacrene A (9.42%), in addition to the previously mentioned germacrene D. The stems and leaves EOs showed capacity to inhibit the Gram-negative Enterobacter aerogenes and the stems EO capacity to inhibit Staphylococcus aureus, with MIC values of 31.2 µg/mL. S. aureus was moderately sensitive to leaves EO, while stems EO displayed moderate activity against Enterococcus faecalis and Salmonella typhimurium (MIC values of 62.5 µg/mL). Candida glabrata was highly susceptible to both EOs (MIC values < 3.9 µg/mL). The EOs showed moderate potential to inhibit the growth of Cryptococcus gatti and Cryptococcus neoformans (MICs of 62.5 μg/mL). Conclusion: The A. triangularis essential oils from stems and leaves displayed capacity to inhibit Enterobacter aerogenes (MIC values of 31.2 µg/mL) and high antifungal effect against Candida glabrata (MIC values of <3.9 µg/mL). Mycobacterium massiliense and M. abscessus were susceptible to the leaves EO, with MICs of 39.06 μg/mL. These results showed the A. triangularis essential oils potential as antifungal and antimycobacterial to be used in the development of new antibiotic.


Coatings ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 980 ◽  
Author(s):  
Dorra Dridi ◽  
Aicha Bouaziz ◽  
Sondes Gargoubi ◽  
Abir Zouari ◽  
Fatma B’chir ◽  
...  

We report an analysis of chemical components of essential oils from barks of Ceylon cinnamon and cloves of Syzygium aromaticum and an investigation of their antibacterial activity. The components of oils were determined by using Gas Chromatography/Mass Spectrometry (GC-MS) analysis, and the antimicrobial activity was assessed by the disk diffusion test. The synergic effect of essential oils mixture (cinnamon oil and clove oil) was evaluated. Antimicrobial properties were conferred to cellulosic fibers through microencapsulation using citric acid as a green binding agent. Essential oil mixture was encapsulated by coacervation using chitosan as a wall material and sodium hydroxide as a hardening agent. The diameter of the produced microcapsules varies between 12 and 48 μm. Attachment of the produced microcapsules onto cotton fabrics surface was confirmed by Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FTIR) spectroscopy, optical microscopy and Scanning Electron Microscopy (SEM) analysis. The results show that microcapsules were successfully attached on cotton fabric surfaces, imparting antibacterial activity without significantly affecting their properties. The finished cotton fabrics exhibited good mechanical properties and wettability.


2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.


2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Shiva Masoudi ◽  
Abdolhossein Rustaiyan ◽  
Razieh Mohebat ◽  
Mohammad Hossein Mosslemin

Water-distilled essential oils from leaves of Hymenocrater yazdianus Rech.f., flowers of Stachys obtusicrena Boiss., and stems and flowers of Nepeta asterotricha Rech.f, which are endemic to Iran, were analyzed by GC and GC/MS. Fifty-five components of the leaf oil of H. yazdianus were characterized, representing 95.1% of the total components detected. The major constituents were identified as 1,8-cineole (17.6%), β-caryophyllene (13.9%), α-pinene (10.6%) and caryophyllene oxide (10.4%). Germacrene-D (37.5%) and α-bisabolol (23.5%) were the main components among the twenty constituents characterized in the flower oil of S. obtusicrena, representing 90.8% of the total components detected. Thirty-five compounds representing 93.0% of the stem oil of N. asterotrica were identified among which terpinen-4-ol (22.8%) and γ-terpinene (14.1%) were the major ones. The flower oil of the species was characterized by higher amounts of terpinen-4-ol (24.8%), 4aα, 7aβ-nepetalactone (18.2%) and 1,8-cineole (11.6%) among the thirty-three components comprising 98.5% of the total oil detected. The antibacterial activity of the stem, leaf and flower oils of Hymenocrater yazdianus, Stachys obtusicrena and Nepeta asterotricha against seven Gram-positive and Gram-negative bacteria were determined using the MIC method. The growth inhibitory zone (mm) was also measured.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat ◽  
Hajar Sadeghi ◽  
Khodamali Oji

The water distilled essential oils from leaves, stems and roots of Chrysanthemum parthenium (L.) Bernh. were analyzed by GC and GC/MS methods. The leaf oil was characterized by a high amount of camphor (56.4%), whereas in the stem oil, camphor (26.0%), trans-β-ocimene (23.6%) and germacrene-d (15.0%) were the major constituents. The main components of the root oil were α-pinene (50.0%), trans-β-farnesene (13.8%) and bicyclogermacrene (11.0%). Antibacterial activity of the leaf, stem and root oil were evaluated using the microdilution broth method. The oils showed inhibitory effects on Escherichia coli and Salmonella typhi, but were not active against Staphylococcus aureus.


Sign in / Sign up

Export Citation Format

Share Document