scholarly journals MORPHOLOGICAL REORGANIZATION OF THE RATS SUBMANDIBULAR GLAND AFTER 2 AND 4 WEEKS OF EXPERIMENTAL STREPTOZOTOCIN-INDUCED DIABETES MELLITUS

2018 ◽  
Vol 17 (3) ◽  
pp. 29-37
Author(s):  
N. B. Blyshchak ◽  
R. Ya. Borys ◽  
U. M. Halyuk
Keyword(s):  
Diabetes Mellitus ◽  
Submandibular Gland ◽  
Packing Density ◽  
Structural Changes ◽  
Male Rats ◽  
White Rats ◽  
Trophic Activity ◽  
Gland Tissue ◽  
Streptozotocin Induced Diabetes ◽  
And Control

This article represents the results of studying the morphological features of the submandibular gland in 40 male rats during experimental streptozotocin-induced diabetes mellitus at the end of 2 and 4 weeks. Destructive changes of parenchymal and stromal elements in the submaxillary salivary glands of white rats and the vessels of the microvasculature were determined beginning from 2 weeks with the increase to 4 weeks of experimental diabetes mellitus. There is a statistically reliable decrease in the diameter of the organ artery, an increase in the index of the trophic activity of the submandibular gland tissue, and decrease in the packing density index of the capillaries. Reliable decrease of the diameter of the interlobular arterioles, the diameter of the intralobular (front capillary) arterioles and capillaries is observed. These changes are confirmed by morphometric parameters: a statistically evidenced narrowing of the diameter of the organ artery was observed to (62.50±3.30) μm, an increase, in comparison with the norm and control, to the index of the trophic activity of the submandibular gland to (58.27±0.71) μm, and decrease in the packing density of the capillaries to (72.00±6.33) μm. A reliable narrowed diameter of the interlobular arterioles to (31.60±1.61) μm, the diameter of the intralobular (per-capillary) arterioles to (18.04±0.28) μm and capillaries to (5.14±0.09) μm was observed. A little dilatation of post-capillary venules to (23.52±0.43) μm was observed. After 4 weeks of the experiment, the rate of disrupt of the angiographic relief of the submandibular gland arise, and the arteries and arterioles remain narrowed. In some places, the formation of shaped elements beyond the border of capillaries is present. Compared with the previous stage of the research, a mild dilatation of the diameter of the organ artery up to (70.06±2.43) μm, dilatation of the diameter of the interlobular arterioles to (36.06±1.01) μm and the diameter of the intralobular (front capillary) arterioles up to (18 26±0.64 μm, the dilatation of the diameter of the capillaries to (5.71±0.13) μm, the control (5.91±0.19) μm and the dilatation, compared with the norm and control, post-capillar venules to (26, 35 ± 0.50) µm were determined. The index of the trophic activity of the gland tissue decreases as compared with the indication for 2 weeks during the experiment to (58.27±0.71) μm and the capillary packing density gradually decreases to (65.8±1.84) μm. Thus, under conditions of streptozotocin-induced diabetes mellitus, the first structural changes in the bloodstream occur after 2 weeks are observed: a statistically significant decrease in the diameter of the organ artery, an increase trophic activity of the submandibular gland tissue and decrease in the capillary packing density in comparison with the norm. A reliable decrease in the diameter of the interlobular arterioles, the diameter of the intralobular (front capillary) arterioles and capillaries is observed. At the same time, a mild dilatation of post-capillary venules occurs. Interlobular arterioles become anfractuous, have an uneven color. After 4 weeks of the experiment, the temp of disrupt of the angiographic relief of the submandibular gland arise, and the arteries and arterioles remain narrowed. In some places, the formation of shaped elements beyond the border of capillaries is observed.

scholarly journals Features of angioarchitectonics and structure of the aortic wall of white rats in the early stages of experimental diabetes mellitus

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 167-174
Author(s):  
M.N. Tsytovskyi ◽  
M.V. Logash ◽  
I.I. Savka ◽  
G.M. Dmytriv
Keyword(s):  
Diabetes Mellitus ◽  
Aortic Wall ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes ◽  
Atherosclerotic Cardiovascular Diseases ◽  
Imagej Software

Background. Atherosclerotic cardiovascular diseases, as well as coronary heart disease, cerebrovascular disease, and peripheral artery disease (probably caused by atherosclerosis), are the leading cause of disability and mortality in people with diabetes. Objective. The purpose of our study was to determine the histostructural features and morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed after 2 and 4 weeks of streptozotocin-induced diabetes mellitus. Methods. The samples for the histology were the sections of the wall of the ascending part, the aortic arch, and the descending part of the aorta of 26 mature white male rats weighing 100 - 160 g. For morphometric examination, a series of images of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 eyepiece. Results and conclusion. The measurements were carried out using the ImageJ software. The development of micro- and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically proved. Statistical analysis revealed a significant difference of all morphometric parameters of both - the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 4 weeks of experimental diabetes in comparison with the norm, control, and the 2-nd week of the experiment. An explicit dependency of the severity of destructive changes in the wall of the aorta and links of its hemomicrocirculatory bed of vessels on the duration of the experiment was determined.

scholarly journals УЛЬТРАСТУКТУРНА ОРГАНІЗАЦІЯ ГЕМОМІКРОЦИРКУЛЯТОРНОГО РУСЛА ШКІРИ БІЛОГО ЩУРА НА РАННІХ ЕТАПАХ ПЕРЕБІГУ ЕКСПЕРИМЕНТАЛЬНОГО СТРЕПТОЗОТОЦИН- ІНДУКОВАНОГО ЦУКРОВОГО ДІАБЕТУ

2019 ◽  
Vol 1 (11(41)) ◽  
pp. 17-22
Author(s):  
Борис Р. Я. ◽  
Блищак Н. Б. ◽  
Михалевич М. М. ◽  
Блищак Ю. З. ◽  
Покотило П. Б.
Keyword(s):  
Diabetes Mellitus ◽  
Endothelial Cells ◽  
Body Weight ◽  
Blood Vessels ◽  
Male Rats ◽  
European Convention ◽  
Standard Diet ◽  
Type I ◽  
White Rats ◽  
Streptozotocin Induced Diabetes

This article is presented information about electron microscopic characteristic the angioarchitectonics of hemomicrocirculatory white rat skin net in experimental streptozotocin-induced diabetes mellitus. Was used in the experiment 30 adult white male rats weighing 120-130 grams, which were maintained on a standard diet, had free access to food and water under normal conditions. The insulin-dependent form of type I diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin from Sigma at a rate of 7mg per 100g of body weight of the animal (prepared on 0.1M citrate buffer, pH = 4.5). The development of experimental diabetes mellitus during 4 weeks was monitored by observing an increase in blood glucose, which was measured by the glucose oxidase method. Studies were performed on rats with glucose levels of 12.00 mmol/l and above. The experiment was carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), Council of Europe Directive 86/609 / EEC (1986). Rats were removed from the experiment by an overdose of intraperitoneal anesthesia using sodium thiopental (calculated at 25 mg / kg body weight of the animal). Applying the method of electron microscopy (the study and photographing of the material was carried out on an electron microscope EM-100 AK at an accelerating voltage of 75 kV and magnification 6000-8000 times). As a material for this examination, the skin intact from the internal surface of the thigh and the back of the white rats was used. The study showed that after 2 weeks of diabetes occure gradually and are progressing alterations of the skin's hemomicrocirculatory bed of white rats, which characterised mainly by small spasm of some vessels. The wall of the most vessels still had normal structure but revealed a narrowing of the capillaries due to the protrusion of the nuclear zone of the endothelial cells. After 4 weeks of the experimental streptozotocin-induced diabetes mellitus were already observed reconstruction practicaly all skin's microvessels of white rats. The lumen of the capillaries became irregular. The contours of the nuclei of endotheliocytes were elongated with an uniform homogen chromatyn, which is concentrated in lumps, thickened bazal membrane, observed proliferation of endothelial cells, which leads to narrowing of the lumen of blood vessels. At the end of the experiment, we observe the inclusion of compensatory properties of the organism - the wall of blood vessels of the hemomicrocirculatory bed of the skin thickens, while the lumen of the microvessels become narrows. The results of investigation can be used in the practice medicine for diagnosis and treatment of skin's diseases in diabetes mellitus.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

scholarly journals Comparative characteristics of the manifestations of damage and reparative processes in the mucous membrane of the duodenum of rats under the conditions of skin burns and skin burns associated with diabetes

2020 ◽  
Vol 26 (2) ◽  
pp. 39-44
Author(s):  
I.A. Tymoshenko ◽  
L.M. Sokurenko ◽  
A.Ya. Yanchyshyn ◽  
V.A. Pastukhova
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Structural Changes ◽  
Burn Injury ◽  
Experimental Diabetes ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Buffer Solution ◽  
Solution Ph

Currently, severe thermal injury is becoming one of the most important problems of practical medicine. Diabetes is also recognized as another global medical and social challenge of our century. The emergency situation for the treatment and prevention of the consequences of these pathologies is a consequence of the lack of a reliable theoretical basis for solving specific clinical problems regarding the course of burns, diabetes and their complications. The aim of the study is to establish the patterns of structural changes in the mucous membrane of the duodenum after burn injury of the skin of rats under conditions of experimental diabetes mellitus. The study was performed on 63 laboratory white adult male rats weighing 180-210 g, which were divided into 3 groups: intact animals, rats with skin burns and rats with skin burns on the background of diabetes. The model of experimental diabetes mellitus was reproduced by administering Streptozotocin to rats intraperitoneally once at a dose of 50 mg/kg, pre-dissolved in 0.1 M citrate buffer solution (pH=4.5). The control of the development of hyperglycemia in the experimental groups was the level of glucose in the blood 24.24±0.79 mmol/l. In the control group this index was 8.03±0.4 mmol/l. Rats with skin burns revealed destructive manifestations, which are accompanied by an active inflammatory reaction and corresponding necrotic changes, while rats with skin burns on the background of diabetes mellitus pathological processes are not just “summed up”, but in some way adaptively modified with the involvement of stress mechanisms of the endoplasmic reticulum and associated autophagy.

Two different models of streptozotocin-induced diabetes mellitus development in Wistar male rats – increased cardiovascular- and liver risk

2018 ◽  
Vol 32 ◽  
pp. 40
Author(s):  
Slavica Mutavdzin ◽  
Jovana Jakovljevic Uzelac ◽  
Jovan Despotovic ◽  
Sanja Stankovic ◽  
Milica Labudovic Borovic ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Male Rats ◽  
Streptozotocin Induced Diabetes ◽  
Wistar Male Rats

scholarly journals Effect of long-term hyperglycemia on cornea and retina morphology in rats with streptozotocin-induced diabetes mellitus

2021 ◽  
pp. 42-47
Author(s):  
А.А. Панов ◽  
Е.М. Ржавина ◽  
М.П. Морозова ◽  
А.К. Ердяков ◽  
С.А. Гаврилова
Keyword(s):  
Diabetes Mellitus ◽  
Ketone Bodies ◽  
Morphological Changes ◽  
Posterior Part ◽  
Male Rats ◽  
Control Group ◽  
Blood Concentrations ◽  
Central Retina ◽  
Streptozotocin Induced Diabetes

Цель исследования - изучение динамики морфологических изменений роговицы и заднего отдела глаза крыс при длительной гипергликемии. Методика. Исследование выполнено на 36 самцах крыс Wistar. Сахарный диабет индуцировали внутрибрюшинной инъекцией стрептозотоцина (65 мг/кг), после чего ежедневно вводили подкожно малые дозы инсулина (2 ЕД/кг). На 50-е, 58-е и 66-е сут эксперимента производили энуклеацию глаз у глубоко наркотизированных животных. Гистологические срезы фрагментов глаз окрашивали гематоксилин-эозином, проводили морфометрию параметров роговицы и сетчатки. Результаты. Средняя концентрация глюкозы и кетоновых тел крови в группе сахарного диабета составила 29,8 ммоль/л и 0,889 ммоль/л, в контрольной группе - 6,2 ммоль/л и 0,847 ммоль/л соответственно. Анализ гистологических срезов глаз выявил признаки отека роговицы, хориоидеи и наружных слоев центральных отделов сетчатки до появления других качественных и количественных морфологических изменений. Заключение. Оценка толщины роговицы, хориоидеи и наружных слоев центральных отделов сетчатки может служить предиктором развития диабетической ретинопатии. The aim was to study morphological changes in the cornea and the posterior part of rat eye during prolonged hyperglycemia. Methods. The study was performed on 36 Wistar male rats. Diabetes mellitus was induced by an injection of streptozotocin (65 mg/kg, i.p.) followed by daily injections of low doses of insulin (2 U/kg, s.c.). Eyes were enucleated from deeply anesthetized rats on days 50, 58, and 66 of the experiment. Histologic sections were stained with hematoxylin-eosin, and morphometry of the cornea and the retina was performed. Results. Mean blood concentrations of glucose and ketone bodies were 29.8 mmol/L and 0.889 mmol/L, respectively, in the diabetic group and 6.2 mmol/L and 0.847 mmol/L, respectively, in the control group. The histological analysis revealed signs of edema in the cornea, choroid and outer layers of the central retina, which preceded other morphological changes. Conclusion. Evaluating thickness of the cornea, choroid and outer layers of the central retina may serve for prediction of diabetic retinopathy.

scholarly journals HISTOLOGICAL AND MORPHOMETRIC ASPECTS OF STRUCTURE OF AORTIC WALL AND ITS HEMOMICROCIRCULATORY BED IN LATE STAGES OF EXPERIMENTAL DIABETES MELLITUS

2021 ◽  
Vol 21 (3) ◽  
pp. 222-228
Author(s):  
M.N. Tsitovskyi ◽  
M.V. Logash ◽  
I.I. Savka
Keyword(s):  
Diabetes Mellitus ◽  
Cardiovascular Diseases ◽  
Aortic Wall ◽  
Social Issues ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
World Health ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes

According to the World Health Organization, cardiovascular diseases and diabetes mellitus occupy a significant niche in the structure of diseases with high disability and mortality impact and pose major healthcare and social issues. It should be stressed that 50-80% of fatal cases in patients with diabetes are associated with cardiovascular diseases. The purpose of this study is to determine the histostructural characteristics and to perform morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed of the aorta in 6 and 8 weeks of streptozotocin-induced diabetes mellitus. The material for the histological study included the sections of the wall of the ascending aorta, the aortic arch, and the descending aorta taken from 26 sexually mature white male rats weighing 100 - 160 g. For morphometric examination, a series of photos of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 ocular. The measurements were carried out using the Image J software. The development of micro - and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically confirmed. Statistical analysis revealed a significant difference in all morphometric parameters of the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 8 weeks of experimental diabetes as compared with the normal values, control values, and in values obtained at the 6-week period of the experiment. The study has demonstrated clear dependence between the severity of destructive changes in the aortic wall and sections of its hemomicrocirculatory bed and the duration of the experiment.

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