scholarly journals УЛЬТРАСТУКТУРНА ОРГАНІЗАЦІЯ ГЕМОМІКРОЦИРКУЛЯТОРНОГО РУСЛА ШКІРИ БІЛОГО ЩУРА НА РАННІХ ЕТАПАХ ПЕРЕБІГУ ЕКСПЕРИМЕНТАЛЬНОГО СТРЕПТОЗОТОЦИН- ІНДУКОВАНОГО ЦУКРОВОГО ДІАБЕТУ

2019 ◽  
Vol 1 (11(41)) ◽  
pp. 17-22
Author(s):  
Борис Р. Я. ◽  
Блищак Н. Б. ◽  
Михалевич М. М. ◽  
Блищак Ю. З. ◽  
Покотило П. Б.
Keyword(s):  
Diabetes Mellitus ◽  
Endothelial Cells ◽  
Body Weight ◽  
Blood Vessels ◽  
Male Rats ◽  
European Convention ◽  
Standard Diet ◽  
Type I ◽  
White Rats ◽  
Streptozotocin Induced Diabetes

This article is presented information about electron microscopic characteristic the angioarchitectonics of hemomicrocirculatory white rat skin net in experimental streptozotocin-induced diabetes mellitus. Was used in the experiment 30 adult white male rats weighing 120-130 grams, which were maintained on a standard diet, had free access to food and water under normal conditions. The insulin-dependent form of type I diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin from Sigma at a rate of 7mg per 100g of body weight of the animal (prepared on 0.1M citrate buffer, pH = 4.5). The development of experimental diabetes mellitus during 4 weeks was monitored by observing an increase in blood glucose, which was measured by the glucose oxidase method. Studies were performed on rats with glucose levels of 12.00 mmol/l and above. The experiment was carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), Council of Europe Directive 86/609 / EEC (1986). Rats were removed from the experiment by an overdose of intraperitoneal anesthesia using sodium thiopental (calculated at 25 mg / kg body weight of the animal). Applying the method of electron microscopy (the study and photographing of the material was carried out on an electron microscope EM-100 AK at an accelerating voltage of 75 kV and magnification 6000-8000 times). As a material for this examination, the skin intact from the internal surface of the thigh and the back of the white rats was used. The study showed that after 2 weeks of diabetes occure gradually and are progressing alterations of the skin's hemomicrocirculatory bed of white rats, which characterised mainly by small spasm of some vessels. The wall of the most vessels still had normal structure but revealed a narrowing of the capillaries due to the protrusion of the nuclear zone of the endothelial cells. After 4 weeks of the experimental streptozotocin-induced diabetes mellitus were already observed reconstruction practicaly all skin's microvessels of white rats. The lumen of the capillaries became irregular. The contours of the nuclei of endotheliocytes were elongated with an uniform homogen chromatyn, which is concentrated in lumps, thickened bazal membrane, observed proliferation of endothelial cells, which leads to narrowing of the lumen of blood vessels. At the end of the experiment, we observe the inclusion of compensatory properties of the organism - the wall of blood vessels of the hemomicrocirculatory bed of the skin thickens, while the lumen of the microvessels become narrows. The results of investigation can be used in the practice medicine for diagnosis and treatment of skin's diseases in diabetes mellitus.

scholarly journals Features of angioarchitectonics and structure of the aortic wall of white rats in the early stages of experimental diabetes mellitus

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 167-174
Author(s):  
M.N. Tsytovskyi ◽  
M.V. Logash ◽  
I.I. Savka ◽  
G.M. Dmytriv
Keyword(s):  
Diabetes Mellitus ◽  
Aortic Wall ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes ◽  
Atherosclerotic Cardiovascular Diseases ◽  
Imagej Software

Background. Atherosclerotic cardiovascular diseases, as well as coronary heart disease, cerebrovascular disease, and peripheral artery disease (probably caused by atherosclerosis), are the leading cause of disability and mortality in people with diabetes. Objective. The purpose of our study was to determine the histostructural features and morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed after 2 and 4 weeks of streptozotocin-induced diabetes mellitus. Methods. The samples for the histology were the sections of the wall of the ascending part, the aortic arch, and the descending part of the aorta of 26 mature white male rats weighing 100 - 160 g. For morphometric examination, a series of images of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 eyepiece. Results and conclusion. The measurements were carried out using the ImageJ software. The development of micro- and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically proved. Statistical analysis revealed a significant difference of all morphometric parameters of both - the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 4 weeks of experimental diabetes in comparison with the norm, control, and the 2-nd week of the experiment. An explicit dependency of the severity of destructive changes in the wall of the aorta and links of its hemomicrocirculatory bed of vessels on the duration of the experiment was determined.

scholarly journals MORPHOLOGICAL REORGANIZATION OF THE RATS SUBMANDIBULAR GLAND AFTER 2 AND 4 WEEKS OF EXPERIMENTAL STREPTOZOTOCIN-INDUCED DIABETES MELLITUS

2018 ◽  
Vol 17 (3) ◽  
pp. 29-37
Author(s):  
N. B. Blyshchak ◽  
R. Ya. Borys ◽  
U. M. Halyuk
Keyword(s):  
Diabetes Mellitus ◽  
Submandibular Gland ◽  
Packing Density ◽  
Structural Changes ◽  
Male Rats ◽  
White Rats ◽  
Trophic Activity ◽  
Gland Tissue ◽  
Streptozotocin Induced Diabetes ◽  
And Control

This article represents the results of studying the morphological features of the submandibular gland in 40 male rats during experimental streptozotocin-induced diabetes mellitus at the end of 2 and 4 weeks. Destructive changes of parenchymal and stromal elements in the submaxillary salivary glands of white rats and the vessels of the microvasculature were determined beginning from 2 weeks with the increase to 4 weeks of experimental diabetes mellitus. There is a statistically reliable decrease in the diameter of the organ artery, an increase in the index of the trophic activity of the submandibular gland tissue, and decrease in the packing density index of the capillaries. Reliable decrease of the diameter of the interlobular arterioles, the diameter of the intralobular (front capillary) arterioles and capillaries is observed. These changes are confirmed by morphometric parameters: a statistically evidenced narrowing of the diameter of the organ artery was observed to (62.50±3.30) μm, an increase, in comparison with the norm and control, to the index of the trophic activity of the submandibular gland to (58.27±0.71) μm, and decrease in the packing density of the capillaries to (72.00±6.33) μm. A reliable narrowed diameter of the interlobular arterioles to (31.60±1.61) μm, the diameter of the intralobular (per-capillary) arterioles to (18.04±0.28) μm and capillaries to (5.14±0.09) μm was observed. A little dilatation of post-capillary venules to (23.52±0.43) μm was observed. After 4 weeks of the experiment, the rate of disrupt of the angiographic relief of the submandibular gland arise, and the arteries and arterioles remain narrowed. In some places, the formation of shaped elements beyond the border of capillaries is present. Compared with the previous stage of the research, a mild dilatation of the diameter of the organ artery up to (70.06±2.43) μm, dilatation of the diameter of the interlobular arterioles to (36.06±1.01) μm and the diameter of the intralobular (front capillary) arterioles up to (18 26±0.64 μm, the dilatation of the diameter of the capillaries to (5.71±0.13) μm, the control (5.91±0.19) μm and the dilatation, compared with the norm and control, post-capillar venules to (26, 35 ± 0.50) µm were determined. The index of the trophic activity of the gland tissue decreases as compared with the indication for 2 weeks during the experiment to (58.27±0.71) μm and the capillary packing density gradually decreases to (65.8±1.84) μm. Thus, under conditions of streptozotocin-induced diabetes mellitus, the first structural changes in the bloodstream occur after 2 weeks are observed: a statistically significant decrease in the diameter of the organ artery, an increase trophic activity of the submandibular gland tissue and decrease in the capillary packing density in comparison with the norm. A reliable decrease in the diameter of the interlobular arterioles, the diameter of the intralobular (front capillary) arterioles and capillaries is observed. At the same time, a mild dilatation of post-capillary venules occurs. Interlobular arterioles become anfractuous, have an uneven color. After 4 weeks of the experiment, the temp of disrupt of the angiographic relief of the submandibular gland arise, and the arteries and arterioles remain narrowed. In some places, the formation of shaped elements beyond the border of capillaries is observed.

scholarly journals Determination of acute toxicity parameters of the drug ‘MEGASTOP for dogs’ on white rats and mice

2020 ◽  
Vol 6 (2) ◽  
pp. 20-26
Author(s):  
O. L. Orobchenko ◽  
M. Ye. Romanko ◽  
M. O. Yaroshenko ◽  
I. O. Gerilovych ◽  
N. A. Zhukova ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Clinical Symptoms ◽  
Liver Volume ◽  
Male Rats ◽  
Toxic Substances ◽  
Main Experiment ◽  
Polyethylene Glycol 400 ◽  
White Rats ◽  
Rats And Mice

The experiments were performed on 58 males of nonlinear white rats 3–4 months old and weighing 180–200 g and 64 females of nonlinear white mice 2.5–3 months old and weighing 18–22 g. In the main experiment on rats, six experimental groups were formed, the animals of which were injected intragastrically with the drug ‘MEGASTOP for dogs’ (by absolute weight) in doses of 1,000.0, 2,000.0, 3,000.0, 4,000.0, 5,000.0, and 6,000.0 mg/kg body weight; in the main experiment on mice, seven experimental groups were formed, the animals of which were administered the drug in doses of 100.0, 500.0, 1,000.0, 1,500.0, 2,000.0, 2,500.0, and 3,000.0 mg/kg body weight. Control rats and mice were injected with 2.0 cm3 and 0.2 cm3 of polyethylene glycol-400, respectively. Clinical symptoms of poisoning with the drug ‘MEGASTOP for dogs’ of white rats (at doses of 2,000.0–6,000.0 mg/kg body weight) and mice (at doses of 1,000.0–3,000.0 mg/kg body weight) were refusals of food and water, loss of coordination, sitting in one place, a dose-dependent increase in depression with subsequent complete depression, lack of response to external stimuli and death on the first or fourth day after administration. During autopsy in rats and mice that died as a result of poisoning with the drug ‘MEGASTOP for dogs’, we recorded pallor of the mucous membranes of the mouth, trachea, pharynx, and esophagus; increase in heart volume, atrial blood supply; pulmonary hyperemia; uncoagulated blood; increase in liver volume, dark cherry color, flabby consistency; catarrhal inflammation of the mucous membrane of the small intestine. According to the results of determining the parameters of acute toxicity of the drug ‘MEGASTOP for dogs’ in the case of a single intragastric injection, LD50 for male rats is 3,384.98 ± 444.94 mg/kg, and for female mice — 2,025.88 ± 279.46 mg/kg body weight, which allows to classify it to class IV by the toxicity — low-toxic substances (LD50 — 501–5,000 mg/kg) and by the degree of danger to class III— moderately dangerous substances (LD50 — 151–5,000 mg/kg)

scholarly journals Toxicity assessment of a technical product of the triazole class

2020 ◽  
Vol 99 (11) ◽  
pp. 1276-1279
Author(s):  
Valery N. Rakitskii ◽  
Tatiana M. Epishina ◽  
Elena G. Chkhvirkiya
Keyword(s):  
Body Weight ◽  
The Body ◽  
Male Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
High Biological Activity ◽  
Experimental Animals ◽  
Technical Product ◽  
White Rats ◽  
Toxicological Studies

Introduction. Historically, pesticides are evaluated more strictly from a medical point of view than other chemicals. Since their features, such as deliberate introduction into the environment, the possibility of contact with them by large masses of the population, and the high biological activity determine their potential danger to humans. Purpose of research - study of the biological effect of a technical product derived from triazoles when it is repeatedly ingested orally in mammals (rats), establishment of inactive and active doses, justification of the permissible daily dose (DSD) for humans. Material and methods. In acute experiments, white rats were used, including 6 animals in the group. Tested dose: 500-4000 mg/kg of body weight. A chronic (12 months) experiment was performed on 80 male rats with a bodyweight of 180-190 g at the beginning of the study. Tested doses: 5.0; 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental animals, 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water, and food consumption, recorded the timing of death, changes in body weight, physiological, biochemical, and hematological indices. Results. Indices of the acute oral toxicity on the studied product LD50 male rats were 2250 ± 483 mg/kg body weight. The dose of 5.0 mg / kg of body weight was not found to cause significant changes in all studied indices. The doses of 16.0 and 55.0 mg/kg of body weight had a polytropic effect on the body in experimental animals. Discussion. The studied product for the acute oral toxicity refers to low-hazard compounds, the doses of 16.0 and 55.0 mg/kg of body weight has a polytropic effect on the mammalian body, causing changes in carbohydrate, lipid, and lipoprotein metabolism in the body of rats - was accepted as acting. The dose of 5.0 mg / kg of body weight, when administered in rats, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. Based on the inactive dose-5.0 mg/kg of body weight and taking into account the reserve factor of 100, we have scientifically justified DSD for a person at the level of 0.05 mg/kg. Summary. The conducted sanitary and Toxicological studies indicate the need to assess the toxicity of new technical products to the mammalian body, to increase the reliability of the developed hygiene standards in environmental objects and food products.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

scholarly journals Effects of lifelong intervention with an oligofructose-enriched inulin in rats on general health and lifespan

2008 ◽  
Vol 100 (6) ◽  
pp. 1192-1199 ◽  
Author(s):  
Pascale Rozan ◽  
Amine Nejdi ◽  
Sophie Hidalgo ◽  
Jean-François Bisson ◽  
Didier Desor ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Biological Markers ◽  
Female Rats ◽  
Male Rats ◽  
Standard Diet ◽  
Male And Female ◽  
Reduced Body ◽  
Male And Female Rats ◽  
And Biological Markers

Ageing is associated with changes in physiology and morphology; nutritional strategies to decrease morbidity and to prolong life are of high interest. The aim of the study was to investigate the effects of lifelong supplementation with an oligofructose-enriched inulin on morphological and biological markers and lifespan in male and female rats. Male and female rats, age 3 months, were randomised into two groups to receive either a diet with 10 % of an oligofructose-enriched inulin (Synergy1) or a standard diet (control) for 27 months. The rats were weighed every 2 weeks and their food intake was evaluated on four successive days every 4–6 weeks. Samples were taken at 12, 18 and 24 months of age. During the whole intervention period, male rats receiving Synergy1 (SYN1-M) displayed lower body weight, cholesterol and plasma triacylglycerolaemia compared with the controls (Cont-M). The survival rate at 24 months of age of SYN1-M rats was 35·3 % greater than that of Cont-M rats. In female rats, the Synergy1 supplementation (SYN1-F) group also reduced body weight, cholesterol and triacylglycerolaemia levels, but results were less consistent over the experiment. The survival rate at 24 months of age in SYN1-F rats was 33·3 % greater compared with that of the control (Cont-F) group. To conclude, lifelong intervention with Synergy1 improved biological markers during ageing and survival rate (lifespan) of rats.

The Effect of Biochanin A as PPAR γ agonist on LDL Particles Diameter and Type 2 Diabetic Dyslipidemia

2019 ◽  
Author(s):  
Darya Ghadimi ◽  
Mohammad Taghi Taghi Goodarzi ◽  
Mahdi Bahmani ◽  
Zohre Khajehahmadi
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Biochanin A ◽  
Diabetic Patients ◽  
Low Density ◽  
Diabetic Dyslipidemia ◽  
Ldl Particles

Background and Aims: Small dense  low-density lipoproteins (sd-LDL) particles are smaller and heavier than typical LDL ones. They can penetrate into the endothelium of coronary arteries more easily because of their small size. Diabetes mellitus is accompanied by dyslipidemia such as increasing concentration of plasma very low density lipoprotein and sd-LDL. Peroxisome proliferator activated receptor γ (PPARγ ) can decrease the level of sd-LDL in plasma. Biochanin A (BCA), a natural compound, is a PPARγ agonist. The present study was designed to investigate the effect of BCA on sd-LDL-Clolesterol level in diabetic animals. Materials and Methods: Adult male rats (Wistar strain) were used as the animal models in this study. Animals were made diabetic by single intraperitoneal injection of Streptozotocin- Nicotinamide and then treated by 1 and 5 mg/kg of BCA for 28 days. Body weight and fasting blood glucose were also tested before and at the end of treatment. Furthermore, the size of LDL particles were measured by nondenaturing polyacrylamide gradient gel electrophoresis assay. Results: Results of the present study indicated that BCA administration at dose of 5mg/kg decreased fasting blood glucose level and increased body weight and diameter of LDL particles in diabetic animals significantly. Conclusions: BCA seems to be an appropriate agent in diabetes mellitus, because it improves the diabetic dyslipidemia, which is the most important complication in diabetic patients.

Sign in / Sign up

Export Citation Format

Share Document