scholarly journals ACTIVITY ETHANOL EXTRACT, ETHYLE ACETATE FRACTION, N-HEXAN FRACTION OF SOFO-SOFO LEAVES (Acmella cf) Against Propionibacterium acnes AND Staphylococcus epidermidis AS ANTIBACTERIES

2021 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Eva Diansari Marbun ◽  
Alfi Sapitri ◽  
Vivi Asfianti
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ash Content ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Propionibacterium Acne ◽  
Extract Content

Sofo-sofo leaves are traditional medicinal plants that have been known by the Nias people to cure fever, coughs, diarrhea and antimicrobial infections on the skin surface. The  purpose  of  this  study  was  to determine the antibacterial activity of ethanol extract, n-hexane fraction and ethylacetate fraction Sofo-sofo leaf (Acmella cf) against Propionibacterium acnes and Staphylococcus epidermidis. Simplicia powder was characterized and phytochemical screening was performed. Furthermore, it was isolated using 96% ethanol solvent and fractionated with  n-hexane and ethylacetate to obtain extracts. Then test the antibacterial activity of each extract against Propionibacterium acne and Staphylococcus epidermidis by using a diffusion method disc paper. The research results obtained water content 5.66%, water soluble extract content 27.33%, ethanol soluble extract content 13.61%, total ash  content 14.39%, and  acid insoluble ash content 6.25%. The highest antibacterial activity was given by ethylacetate extract at a concentration of 2% (23.4 ± 0.2) compared to ethanol extract with a concentration of 2% (21.06 ± 0.85) and 2% concentrated n-hexane extract (19.36 ± 0.16) against the Propionibacterium acne bacteria. The highest antibacterial activity was ethyl acetate extract at a concentration of 2% (23.24 ± 0.23), ethanol extract with a concentration of 2% (16.36 ± 1.76) and n-hexane extract at a concentration of 2% (15.36 ± 0 , 11) against Staphylococcus epidermidis bacteria. The  antibacterial activity results were analyzed by the one way ANOVA test method. Based on these results it can be concluded that there are differences between treatment groups, which is indicated by a significant value <0.05.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MELINJO (Gnetum gnemon L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus dan Streptococcus mutans TAHUN 2020

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Firdaus Fahdi ◽  
Herviani Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Ash Content ◽  
Water Soluble ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Gnetum Gnemon

The Gnetum gnemon can be used as a medicine that has the function of anti-bacterial. The chemical content inside which is anti-bacterial is flavonoids and tannins. This study aims to find out the antibacterial from the ethanol extract of melinjo leaves can inhibit the growth of Staphylococcus aureus and Streptococcus mutans with a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml. The test of anti-bacterial activity for the growth Staphylococcus aureus and Streptococcus mutans is done by using the disc diffusion method. The results showed that melinjo leaf contains an alkaloid, flavonoid, tannin, steroid and saponin compounds. The results of the simplicia powder characteristics obtained a total ash content of 7.495%, ash content insoluble in acid 0.785%, ethanol-soluble extract 4.324%, water-soluble extract 17.690%. The results of the antibacterial activity test of the ethanol extract of melinjo leaves obtained can inhibit the growth of Staphylococcus aureus and Streptococcus mutans namely at a concentration of 300 mg/ml, 400 mg/ml, and 500 mg/ml into the medium inhibition zone category. Ethanol extract of melinjo leaves has antibacterial activity against the growth of Staphylococcus aureus and Streptococcus mutans with an average diameter of inhibition zone of 9.7 mm (medium category) against Staphylococcus aureus and 6.7 mm (medium category) against bacteria Streptococcus mutans.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK MIKROALGA Thalassiosira sp TERHADAP BAKTERI Staphylococcus aureus, Staphylococcus epidermidis dan Propionibacterium Acne

2019 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Vina Juliana Anggraeni ◽  
Titis Setyaning Wahyu ◽  
Herni Kusriani ◽  
Dewi Kurnia
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Propionibacterium Acne ◽  
X 24

AbstrakPengembangan obat dan kosmetik dari biota laut kini tengah terjadi di dunia farmasi. Mikroalga Thalassiosira sp merupakan jenis mikroalga yang memiliki kandungan senyawa-senyawa bioaktif. Beberapa penelitian sebelumnya menunjukkan bawa mikroalga memiliki aktivitas antibakteri.  Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak mikroalga Thalassiosira sp  terhadap 3 bakteri  yaitu staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne di fasa n-heksan, etil asetat dan etanol. Ketiga bakteri ini dapat menyebabkan infeksi kulit. Mikroalga Thalassiosira sp dikultivasi menggunakan medium walne dan di panen pada hari ke-6 setelah penanaman. Pemanen mikroalga dilakukan dengan teknik sentrifuga. Ektrak dilakukan dengan cara  maserasi bertingkat selama 3 x 24 jam. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstrak mikroalga Thalassiosira sp diperoleh paling banyak pada ekstrak etanol sebanyak 24,24%(b/b), ektrak etil asetat sebanyak 19,75%(b/b) dan paling sedikit adalah ekstrak heksan sebanyak 8,64% (b/b). Hasil uji difusi menunjukkan ekstrak n-heksan, etil asetat dan etanol mikroalga Thalassiosira sp memiliki aktivitas terhadap bakteri staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne yang ditunjukan dengan adanya zona bening. Kata kunci: mikroalga, Thalassiosira sp, antibakteri, infeksi kulit, metode difusi Abstract Development drugs and cosmetics from marine biota is now being happened in pharmacy word. Microalgae Thalassiosira sp is a type of microalgae that has a bioactive compounds. Several previous studies have shown the existence of microalgae which have antibacterial activity. This study aimed to study the antibacterial activity of extracts of microalgae Thalassiosira sp against 3 bacteria which is staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne in the n-hexane, ethyl acetate and ethanol phases. These three bacteria can cause skin infections. Microalgae Thalassiosira sp was cultivated using walne medium and harvested on the 7th day after planting. Microalgae harvesters are carried out by centrifuge techniques. The extract is done by multilevel maceration for 3 x 24 hours. Antibacterial testing was carried out by the paper diffusion method or Kirby-Bauer's disc diffusion method. The results of the crude extract of microalgae Thalassiosira sp were obtained at most in ethanol extract as much as 24.24% (w/w), extract of ethyl acetate at 19.75% (w / w) and at least hexane extract at 8.64% (w/w). The diffusion test results for n-hexane, ethyl acetate, and ethanol microalgae Thalassiosira sp extract have activity on  staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne which are supported by clear zones. Keywords: mikroalge, Thalassiosira sp, antibacteria, skin infection, diffusion method

scholarly journals The Antifungal Activity of n-Hexane Extract of Eleutherine palmifolia (L). Merr Bulbs Against Candida albicans and Trichophyton mentagrophytes

2019 ◽  
Vol 7 (22) ◽  
pp. 3777-3780
Author(s):  
Masfria Masfria ◽  
Mitra S. A. Tampubolon
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Mentagrophytes ◽  
Ash Content ◽  
Hexane Extract ◽  
Water Soluble ◽  
Diffusion Method ◽  
Soluble Extract ◽  
Phytochemical Content ◽  
Extract Content

AIM: This research aimed to determine the characteristics of dried bulbs of Eleutherine palmifolia, the group of active chemical compounds contained in n-hexane extract of Eleutherine palmifolia bulbs and the antifungal activity of n-hexane extract of Eleutherine palmifolia bulbs against Candida albicans and Trichophyton mentagrophytes. METHODS: The Eleutherine palmifolia bulbs were extracted by percolation method using n-hexane solvent. The extract was tested for its antifungal activity against Candida albicans and Trichophyton mentagrophytes by diffusion method using paper discs. RESULTS: The characterisation of dried Eleutherine palmifolia bulbs were obtained water content 9.38%, water-soluble extract content 12.15%, ethanol-soluble extract content 14.48%, total ash content 0.91%, and acid insoluble ash content 0.70%. Determination of Phytochemical content showed alkaloid, flavonoid, tannin, saponin, glycoside, and steroid/triterpenoid compounds. The antifungal activity of Eleutherine palmifolia bulbs n-hexane extract (EPBHE) by concentration 200 mg/ml demonstrated the inhibition diameter of 19.48 and 42.20 mm for Candida albicans and Trichophyton metagrophytes, respectively. CONCLUSION: The antifungal test indicates that n-hexane extract of Eleutherine palmifolia bulbs provides inhibitory power to Candida albicans and Trichophyton mentagrophytes.

scholarly journals Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes

2021 ◽  
Vol 6 (1) ◽  
pp. 58528
Author(s):  
Vilya Syafriana ◽  
Renita Noviani Purba ◽  
Yayah Siti Djuhariah
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Etlingera Elatior

This study aimed to determine the antibacterial activity from the ethanol extract of Kecombrang flower (Etlingera elatior (Jack) R.M. Smith) against Staphylococcus epidermidis and Propionibacterium acnes. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the disk diffusion method with a concentration of 10%, 20%, 40%, and80%. Meanwhile, theMinimum InhibitoryConcentration(MIC)was done atconcentrations of 10%, 8%, 6%, 4%, and 2%. The results showed that the Kecombrang flower (Etlingera elatior (Jack) R.M.Smith) extract hadantibacterialactivityagainstS.epidermidisand P. acnes. The MIC for S. epidermidis is at a concentration of 4%, while in P.acnes cannot determine yet.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

scholarly journals THE ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE BULBOSA (MILL.) URB.) FROM CENTRAL KALIMANTAN AGAINST ACNE-CAUSING BACTERIA

2019 ◽  
pp. 22-25
Author(s):  
SUSI NOVARYATIIN ◽  
SYAHRIDA DIAN ARDHANY
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Diffusion Technique ◽  
Inhibition Zones ◽  
Phytochemical Constituents

Objective: The objective of this research was to investigate the antibacterial activity of bawang dayak from Central Kalimantan against acne-causingbacteria namely Propionibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus.Methods: The preliminary phytochemical constituents were qualitatively analyzed. Antibacterial activity of bawang dayak ethanol extract wasperformed using disc-diffusion technique, with five variations of concentration of 1.25%, 2.5%, 5%, 10%, and 20%.Results: Bawang dayak extract contained flavonoids, alkaloids, saponins, and tannins. The inhibition zones of bawang dayak ethanol extract in fivevarious concentrations (1.25%, 2.5%, 5%, 10%, and 20%) were 7.0±1.3 mm, 8.5±0.6 mm, 9.9±0.3 mm, 11.2±0.1 mm, and 11.9±0.3 mm against P. acnes;18.8±3.3 mm, 21.9±3.3 mm, 20.8±0.6 mm, 22.0±0.2 mm, and 23.1±0.6 mm against S. epidermidis; and 14.3±3.1 mm, 13.5±0.9 mm, 14.7±1.5 mm,16.1±1.0 mm, and 20.1±0.6 mm against S. aureus, respectively.Conclusion: This present study showed that bawang dayak ethanol extract was active against all the tested acne-causing bacteria. The highestantibacterial activity was produced by 20% of bawang dayak ethanol extract against S. epidermidis.

scholarly journals Anti Acne Vulgaris Topical Cream From Combination Extract of Gymnanthemum Amygdalinum Del. and Elaeis Guineensis Jacq. Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 42-50
Author(s):  
Wahyudi ◽  
Ayu Syahfitri ◽  
Syukur Berkat Waruwu ◽  
Dian Ika Perbina
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Acne Vulgaris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Propionibacterium Acne ◽  
Self Confidence ◽  
Adolescents And Adults

Acne is one problem in adolescents and adults who can reduce self-confidence. This study aimed to formulate the combination cream of African leaf extract and palm oil leaves to determine the antibacterial activity of African leaf extract against several acne-causing bacteria (Propionibacterium acne and Staphylococcus epidermidis). Test antibacterial activity of extracts and cream with disc diffusion method. The cream is made with concentration of 5, 10 and 20% w/w. The evaluation results of cream with concentration of 5, 10 and 20% were physically stable for 4 weeks of storage at temperature room, homogeneous, pH and it did not cause irritation. Based on the test results, a combination of African leaf extract with concentration of 5% effectively inhibited bacterial growth with an average inhibition zone diameter of 12.06 mm for Propionibacterium acne bacteria and 13.63 mm for Staphylococcus epidermidis bacteria. The result of this study suggest that combination of African leaf extract and palm leaves posses potent anti-bacterial activity against Propionibacterium acne and Staphylococcus epidermidis.

scholarly journals Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan

2020 ◽  
Vol 3 (4) ◽  
pp. 209-215
Author(s):  
Sutomo Sutomo ◽  
Dina Noor Kamali ◽  
Arnida Arnida ◽  
Normaidah Normaidah ◽  
Agung Sriyono
Keyword(s):  
Antioxidant Activity ◽  
Drying Shrinkage ◽  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Extract Content ◽  
Dpph Method ◽  
Specific Parameter ◽  
Parameter Test

Mundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52�0.1%; acid insoluble ash content 1.06�0.08%; drying shrinkage 6.43�0.38%; water-soluble extracts 34.3�0.3%; and ethanol-soluble extracts 23.47�0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity.

scholarly journals ANTIBACTERIAL ACTIVITY OF FRUIT BANANA STONE AND MECHANISM

2018 ◽  
Vol 11 (13) ◽  
pp. 167
Author(s):  
Panal Sitorus ◽  
Dwi Suryanto, Hepni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Clear Zone ◽  
Activity Test ◽  
Cellular Metabolite ◽  
Cell Leakage

Objective: The purpose of this study was to determine the strength of antibacterial activity of the fraction of banana stone and its mechanism.Methods: The antibacterial activity test was performed using the diffusion method by measuring the diameter of the clear zone around the disc paper.Results: The results of antibacterial activity test showed that ethyl acetate fraction was more effective against bacterium Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes compared to n-hexane fraction.Conclusion: The resulting cellular metabolite leak showed that cell leakage in the three bacteria due to ethyl acetate fraction had leaked more protein than nucleic acid, while the leakage of more dissolved K+ ion than Ca2 + ions.

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