scholarly journals Effect of Fertility Blend® Administration on the Epididymal Sperm Function Parameters of Vasectomized Mice: Physiological and Genetical Study as Model for Obstructive Azoospermic Men

2018 ◽  
Vol 12 (1) ◽  
pp. 132-136
Author(s):  
Saad S. Al-Dujaily ◽  
Hazim I. Al-Ahmed ◽  
Jasim Al-Ethawi ◽  
Haidar A. Al-Ebrahimi
Keyword(s):  
Male Fertility ◽  
Treated Group ◽  
Sperm Function ◽  
Obstructive Azoospermia ◽  
Epididymal Sperm ◽  
Nutrient Supplements ◽  
Sperm Characters ◽  
Different Sources

Male Fertility Blend® is  a new  nutrient supplements containing many constituents especially a plant called  Dong quai(Angelia  Sinensis). The plant extract has been used to facilitate the sperm function parameters. However, the studies concern on its effects on epididymal sperms of vasectomized males and obstructive azoospermia are very rare. Thus, this investigation was designed to elucidate the role of Male Fertility Blend® (MFB) formula on the in vivo epididymal sperm characters and DNA fragmentations of vasectomized male mice as a model for man. In this study, the orally administration of 3.4 µg/ml MFB was used for vasectomized and non-vasectomized mice along 35 days. The results revealed a significant (p≤0.05) increment in certain sperm function parameters of vasectomized mice by using fertility Blend® than that of not treated by this supplement. The percentage of progressive and unprogressive active sperm motility using MFB was significantly (P≤0.05) increased compared with non-treated group. It was concluded that the MFB formula containing different sources of energy and variety of factors that sustain the epididymal sperm of healthy and vasectomized mice. Therefore this supplement can be utilized for males complaining from obstructive azoospermia and other factors of infertility.

scholarly journals Staphylococcus aureus Infection Induced Oxidative Imbalance in Neutrophils: Possible Protective Role of Nanoconjugated Vancomycin

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Panchanan Pramanik ◽  
Somenath Roy
Keyword(s):  
Oxidative Stress ◽  
Staphylococcus Aureus ◽  
Cell Damage ◽  
Treated Group ◽  
Protective Role ◽  
Control Group ◽  
Similar Dose ◽  
Cellular Components

Staphylococcus aureus infection causes oxidative stress in neutrophils. The immune cells use reactive oxygen species (ROS) for carrying out their normal functions while an excess amount of ROS can attack cellular components that lead to cell damage. The present study was aimed to test the protective role of nanoconjugated vancomycin against vancomycin-sensitive Staphylococcus aureus (VSSA) and vancomycin-resistant Staphylococcus aureus (VRSA) infection induced oxidative stress in neutrophils. VSSA- and VRSA-infection were developed in Swiss mice by intraperitoneal injection of 5×106 CFU/mL bacterial solutions. Nanoconjugated vancomycin was treated to VSSA- and VRSA-infected mice at its effective dose for 10 days. Vancomycin was treated to VSSA and VRSA infected mice at similar dose, respectively, for 10 days. The result reveals that in vivo VSSA and VRSA infection significantly increases the level of lipid peroxidation, protein oxidation, oxidized glutathione level, and nitrite generation and decreases the level of reduced glutathione, antioxidant enzyme status, and glutathione-dependent enzymes as compared to control group; which were increased or decreased significantly near to normal in nanoconjugated vancomycin-treated group. These finding suggests the potential use and beneficial protective role of nanoconjugated vancomycin against VSSA and VRSA infection induced oxidative imbalance in neutrophils.

scholarly journals Reduced Fertility in Male Mice Deficient in the Zinc Metallopeptidase NL1

2004 ◽  
Vol 24 (10) ◽  
pp. 4428-4437 ◽  
Author(s):  
Mélanie Carpentier ◽  
Christine Guillemette ◽  
Janice L. Bailey ◽  
Guy Boileau ◽  
Lucie Jeannotte ◽  
...  
Keyword(s):  
Male Fertility ◽  
Protein A ◽  
Physiological Role ◽  
Sperm Function ◽  
Secreted Protein ◽  
Wild Type ◽  
The Family ◽  
Fertility Problems ◽  
Targeting Strategy

ABSTRACT Members of the M13 family of zinc metalloendopeptidases have been shown to play critical roles in the metabolism of various neuropeptides and peptide hormones, and they have been identified as important therapeutic targets. Recently, a mouse NL1 protein, a novel member of the family, was identified and shown to be expressed mainly in the testis as a secreted protein. To define its physiological role(s), we used a gene targeting strategy to disrupt the endogenous murine Nl1 gene by homologous recombination and generate Nl1 mutant mice. The Nl1−/− mice were viable and developed normally, suggesting that zygotic expression of Nl1 is not required for development. However, Nl1−/− males produced smaller litters than their wild-type siblings, indicating specific male fertility problems. Reduced fertility may be explained by two impaired processes, decreased egg fertilization and perturbed early development of fertilized eggs. These two phenotypes did not result from gross anatomical modifications of the testis or from impaired spermatogenesis. Basic sperm parameters were also normal. Thus, our findings suggest that one of the roles of NL1 in mice is related to sperm function and that NL1 modulates the processes of fertilization and early embryonic development in vivo.

Fertilizing capacity of epididymal and testicular sperm using intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 281 ◽  
Author(s):  
SJ Silber ◽  
P Devroey ◽  
H Tournaye ◽  
Steirteghem AC Van
Keyword(s):  
Pregnancy Rate ◽  
Intracytoplasmic Sperm Injection ◽  
Pregnancy Rates ◽  
Obstructive Azoospermia ◽  
Motile Sperm ◽  
Epididymal Sperm ◽  
Testicular Sperm ◽  
Cleavage Rate

For men with uncorrectable obstructive azoospermia, their only hope of fathering a child is microsurgical epididymal sperm aspiration (MESA) combined with in vitro fertilization (IVF). In 1988, proximal epididymal sperm were demonstrated to have better motility than senescent sperm in the distal epididymis, and it was thought that retrieval of motile sperm from the proximal epididymis would yield reliable fertilization and pregnancy rates after conventional IVF. However, the results to date have been poor, and although a minority of patients achieved good fertilization rates with IVF, the vast majority (81%) had consistently poor or no fertilization and the pregnancy rate averaged only 9%. Recently, intracytoplasmic sperm injection (ICSI) has been successfully used to achieve fertilization and pregnancies for patients with extreme oligoasthenozoospermia. ICSI has therefore been applied to cases of obstructive azoospermia and, in this report, 67 MESA-IVF cases are compared with 72 MESA-ICSI cases. The principle that motile sperm from the proximal segments of the epididymis should be used for ICSI was followed, although in the most severe cases in which there was an absence of the epididymis (or absence of sperm in the epididymis), testicular sperm were obtained from macerated testicular biopsies. These sperm only exhibited a weak, twitching motion. In 72 consecutive MESA cases, ICSI resulted in fertilization and normal embryos for transfer in 90% of the cases, with an overall fertilization rate of 46%, a cleavage rate of 68%, and ongoing or delivered pregnancy rates of 46% per transfer and 42% per cycle. The pregnancy and take-home baby rates increased from 9% and 4.5% with IVF to 53% and 42% with ICSI. There were no differences between the results for fresh epididymal, frozen epididymal or testicular sperm, and the number of eggs collected did not affect the outcome. The results were also unaffected by the aetiology of the obstruction such as congenital absence of the vas deferens or failed vasoepididymostomy. The only significant factor which affected the pregnancy rate was female age. It is concluded that although complex mechanisms involving epididymal transport may be beneficial for conventional fertilization of human oocytes (in vivo or in vitro), none of these mechanisms are required for fertilization after ICSI. Given the excellent results with epididymal and testicular sperm, ICSI is obligatory for all future MESA patients. Finally, the use of ICSI with testicular sperm from men with non-obstructive azoospermia is also discussed.

scholarly journals Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10662
Author(s):  
Tiranan Buddawong ◽  
Somluk Asuvapongpatana ◽  
Chanyatip Suwannasing ◽  
Valainipha Habuddha ◽  
Chompoonut Sukonset ◽  
...  
Keyword(s):  
Treated Group ◽  
Control Group ◽  
Shell Formation ◽  
Haliotis Diversicolor ◽  
Inner Surface ◽  
Shell Regeneration ◽  
Regeneration Experiment ◽  
Subunit B

Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.

scholarly journals Internalization ofStaphylococcus aureusin Lymphocytes Induces Oxidative Stress and DNA Fragmentation: Possible Ameliorative Role of Nanoconjugated Vancomycin

2011 ◽  
Vol 2011 ◽  
pp. 1-15 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Santanu Kar Mahapatra ◽  
Sumanta Kumar Sahu ◽  
Sabyasachi Das ◽  
Satyajit Tripathy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Staphylococcus Aureus ◽  
Immune Cell ◽  
Bloodstream Infections ◽  
Treated Group ◽  
Control Group ◽  
Similar Dose ◽  
Effective Doses

Staphylococcus aureusis the most frequently isolated pathogen causing bloodstream infections, skin and soft tissue infections and pneumonia. Lymphocyte is an important immune cell. The aim of the present paper was to test the ameliorative role of nanoconjugated vancomycin against Vancomycin-sensitiveStaphylococcus aureus(VSSA) and vancomycin-resistantStaphylococcus aureus(VRSA) infection-induced oxidative stress in lymphocytes. VSSA and VRSA infections were developed in Swiss mice by intraperitoneal injection of  CFU/mL bacterial solutions. Nanoconjugated vancomycin was adminstrated to VSSA- and VRSA-infected mice at its effective dose for 10 days. Vancomycin was adminstrated to VSSA- and VRSA-infected mice at a similar dose, respectively, for 10 days. Vancomycin and nanoconjugated vancomycin were adminstrated to normal mice at their effective doses for 10 days. The result of this study reveals that in vivo VSSA and VRSA infection significantly increases the level of lipid peroxidation, protein oxidation, oxidized glutathione level, nitrite generation, nitrite release, and DNA damage and decreases the level of reduced glutathione, antioxidant enzyme status, and glutathione-dependent enzymes as compared to control group, which were increased or decreased significantly near to normal in nanoconjugated vancomycin-treated group. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VSSA and VRSA infection-induced oxidative stress in lymphocytes.

miR-182-5p Attenuates High-Fat -Diet-Induced Nonalcoholic Steatohepatitis in Mice

2018 ◽  
Vol 17 (5) ◽  
pp. 0-10
Author(s):  
Qionghe Liang ◽  
Huan Chen ◽  
Xiaoqun Xu ◽  
Weiwei Jiang
Keyword(s):  
Nonalcoholic Steatohepatitis ◽  
High Fat Diet ◽  
Hepg2 Cells ◽  
Treated Group ◽  
High Fat ◽  
Protein Levels ◽  
Tlr4 Mrna ◽  
Increased Risk

Introduction and Aim: Patients with NASH have increased risk for sepsis or cardiovascular disease after Liver transplantation. An important role of Toll-like receptor (TLR) 4 in the pathogenesis of nonalcoholic steatohepatitis (NASH) was demonstrated. Here, we study the role of miR-182-5p in TLR4 expression and high-fat-diet (HFD)-induced NASH in vitro and in vivo. Methods: Following transfection with a miR-182-5p mimic, the effect of miR-182-5p on TLR4 in RAW264.7 and HepG2 cells was investigated. Following administration of the miR-182-5p mimic into the livers of HFD-induced NASH mice, we determined the in vivo expression of TLR4, TNFα, and IL-6 and assessed the histologic features of the livers. Results: Following lipopolysaccharide (LPS) treatment of RAW264.7 cells, real-time RT-PCR and western blot results indicated decreases levels of TLR4 mRNA and protein in the miR-182-5p group as compared with levels observed in controls, with similar trends were observed in TNFα and IL-6 protein levels. Following oleic acid (OA) treatment of HepG2 cells, TLR4, TNFα, and IL-6 levels were significantly decreased in the miR-182-5p group as compared with levels observed in controls. Following miR-182-5p administration, TLR4 mRNA and protein levels decreased along with those of TNFα and IL-6 proteins, and the liver weight/body weight ratio of treated mice was less than that observed in controls. Furthermore, hematoxylin and eosin staining showed that the miR-182-5p-treated group exhibited low adipose-cell cross-sectional areas, and Oil Red O staining showed decreases in the size of lipid droplets in the miR-182-5p-treated group. Conclusions: miR-182-5p ameliorated HFD-induced NASH by suppressing TLR4.

Protective role of caffeic acid on lambda cyhalothrin-induced changes in sperm characteristics and testicular oxidative damage in rats

2011 ◽  
Vol 28 (7) ◽  
pp. 639-647 ◽  
Author(s):  
Fatma Ben Abdallah ◽  
Hamadi Fetoui ◽  
Nassira Zribi ◽  
Feiza Fakhfakh ◽  
Leila Keskes
Keyword(s):  
Oxidative Damage ◽  
Caffeic Acid ◽  
Reproductive System ◽  
Male Fertility ◽  
Treated Group ◽  
Protective Role ◽  
Male Reproductive System ◽  
Male Rats ◽  
Lambda Cyhalothrin

The synthetic pyrethroids are expected to cause deleterious effects on most of the organs and especially on the male reproductive system. The current study was performed to assess the adverse effect of lambda cyhalothrin (LC) on reproductive organs and fertility in male rats and to evaluate the protective role of caffeic acid phenethyl ester (CAPE) in alleviating the detrimental effect of LC on male fertility. A total of 48 male rats were divided into 4 groups (12 rats each): control group received distilled water ad libitum and 1 ml of vehicle solution given intraperitoneally (i.p.); CAPE-treated group received a single i.p. dose of CAPE (10 μmol kg−1 day−1); LC-treated group received 668 ppm of LC through drinking water; and CAPE + LC-treated group received an i.p. injection of CAPE (10 μmol kg−1 day−1) 12 h before the LC administration. The experiment was conducted for 10 consecutive weeks. LC caused a significant increase in testicular malondialdehyde, catalase, superoxide dismutase, glutathione- S-transferase activities, and sperm abnormalities and a significant reduction in testicular glutathione concentration, sperm count, sperm motility, and a live sperm percentage. Conversely, treatment with CAPE improved the reduction in the sperm characteristics, LC-induced oxidative damage of testes and the testicular histopathological alterations. Results indicate that LC exerts significant harmful effects on the male reproductive system and that CAPE reduced the deleterious effects of LC on male fertility.

scholarly journals Effect of maca(Lepidium meyenii) aqueous extract on the epididymal sperms quality and the DNA normality of vasectomized mature mice:model forobstructive azoospermia in men

2018 ◽  
Vol 12 (2) ◽  
pp. 73-81
Author(s):  
Saad S. Al-Dujaily ◽  
Jwad K Al-Arak ◽  
Jasim M. Ethawi ◽  
Haider Al-Ebrahimi ◽  
Muna Yosif
Keyword(s):  
Healthy Adult ◽  
Treated Group ◽  
Negative Control ◽  
Sperm Function ◽  
Obstructive Azoospermia ◽  
Lepidium Meyenii ◽  
Adult Mice ◽  
Normal Sperm ◽  
Sperm Dna ◽  
Significant Increment

        This study was aimed to found out the effect of Maca extract on certain sperm function characters and sperm DNA normality in vasectomized and healthy adult mice as a model for men complaining from obstructive azoospermia. Twenty adult male mice were randomly divided into four groups (5males per each group).The first group(GI) was regarded as a negative control that treated with distilled water. Whereas group II  was gavage maca extract daily  with 1mg/100gm.GropIII,and IV were vasectomized . Mice in GIII were vasectomized without treatment while the mice in group GIV were vasectomized and orally administrated maca dose(1mg/100gm).At the last of experiment(35 days), all the mice were scarified for assessment of  certain sperm function parameters . The results revealed that a significant (P<0.05)improvement of  grade (A and B) of active sperm motility in mice treated  orally with  Maca extract was recorded compared with other groups, while grade (C) was significantly increase in vasectomized mice (GIIIgroup) as compared with other healthy mice . DNA fragmentation resulted from fertile mice gavages with maca  were reduced compared to healthy non-treated group and other  treated and non-treated vasectomized mice (GIII and GIVgroup).Orally administration of animals with Maca extract caused  a significant (p<0.05) improvement in  the percentage of  morphologically normal sperm compared with vasectomized  treated and non treated groups. It is concluded that oral administration of  Maca extract caused a  significant increment in  certain sperm function parameters of vasectomized mice in turn this result can be utilized for  obstructive azoospermic men. 

scholarly journals Role of Pistia stratiotes on Radiation Induced Genotoxicity : Analysis of Micronucleus and Chromosome Aberrations in vivo

2013 ◽  
pp. 63-67
Author(s):  
Megha Jha ◽  
Versha Sharma ◽  
Ganesh Narayan
Keyword(s):  
Treated Group ◽  
Pistia Stratiotes ◽  
Micronucleus Induction ◽  
60Co Gamma ◽  
Health Promoting ◽  
Radiation Induced ◽  
60Co Gamma Rays ◽  
60Co Gamma Radiation

The present study aimed to examine the radioprotective potentiality and efficacy of Pistia stratiotes against damage induced by 60Co gamma radiation. Mice were exposed to 4 Gy 60Co gamma rays. MeOH extract (50 and 100 mg/kg body wt) was administered orally with irradiation. Bone marrow protection was evaluated by scoring the different types of individual aberrations, aberrant metaphases and micronuclei formation. Significant reduction in number of aberrant cells and different type of aberrations was observed in treated group compared to irradiated untreated group of animals. The administration of MeOH leaf extract of Pistia stratiotes to the animals showed significant reduction in micronucleus induction. The presence of large amount of two di-Cglycosylflavones of the vicenin and lucenin and lesser amounts of the anthocyanin cyaniding-3-glucoside and a luteolin-7-glycoside, and traces of the mono- C-glycosyl flavones, vitexin and orientin found to be responsible for the observed bioefficacy. Our findings support the use of Pistia stratiotes (ignored weed) as health promoting food and its potential for clinical use.

Sign in / Sign up

Export Citation Format

Share Document