scholarly journals Bioaktivitas Campuran Ekstrak Biji Barringtonia asiatica L. (Kurz.) (Lecythidaceae) dan Getah Azadirachta indica A. Juss. (Meliaceae) terhadap Larva Spodoptera litura F. (Lepidoptera: Noctuidae)

Agrikultura ◽  
2015 ◽  
Vol 26 (1) ◽  
Author(s):  
Indah Meutia Arisanti ◽  
Danar Dono
Keyword(s):  
Azadirachta Indica ◽  
Spodoptera Litura ◽  
Toxicity Testing ◽  
Seed Extract ◽  
Feeding Method ◽  
Pests And Diseases ◽  
Lc50 Value ◽  
Low Toxicity ◽  
Insecticidal Toxicity ◽  
Lepidoptera Noctuidae

ABSTRACTBioactivity of mixed seed extract of Barringtonia asiatica l. (kurz.) (lecythidaceae) and sap of Azadirachtaindica A. Juss. (meliaceae) against larvae of Spodoptera litura f. (lepidoptera: noctuidae)Barringtonia asiatica and Azadirachta indica are known to have variety of chemical compounds that haspotency to be developed as botanical pesticides. The study aimed to determine insecticidal toxicity of B.asiatica seed extract and A. indica sap and their mixture. The experiment was carried out in the Laboratoryof Pesticides and Application Technology, Department of Plant Pests and Diseases, Faculty of Agriculture,Universitas Padjadjaran. The experiments were conducted either on toxicity test of the seed extract of B.asiatica and the sap of A. indica singly or their mixture based on the comparison of LC95. Toxicity testing wasperformed using a leaf-residue feeding method on the instar I of Spodoptera litura larvae. The resultsshowed that seed extractof B.asiatica has a moderate toxicity to S.liturawith the LC50 value of 0.491% andaffected body weight of the larvae. The sap of A.indicahas had slightly low toxicity with the LC50 value of1.310%. The mixture of B. asiatica seed extract and A. Indica sap (based on ratio of 4.1%of B. asiaticacompare to 4.9% of A. Indica) has LC50 value of 0.970% (slightly low toxicity) and the LC95 value of 15.99%that was antagonistic on the LC95 level with Cotoxicity Ratio value of 0.23% on 12 days after treatment.Keywords: Cotoxicity ratio, independent joint action, Barringtonia asiatica, Azadirachta indica, SpodopteralituraABSTRAKBarringtonia asiatica dan Azadirachta indica diketahui memiliki berbagai senyawa kimia yang berpotensidikembangkan sebagai pestisida nabati. Penelitian yang bertujuan untuk mengetahui toksisitas ekstrak biji B.asiatica dan getah A. indica serta campurannya telah dilakukan di Laboraturium Pestisida dan TeknikAplikasi, Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran.Penelitian meliputi uji toksisitas ekstrak biji B. asiatica dan getah A.indica secara tunggal, dan pengujiancampuran ekstrak biji B. asiatica dan getah A.indica berdasarkan pada perbandingan LC95. Pengujiantoksisitas dilakukan dengan metode celup pakan pada larva instar I Spodoptera litura. Hasil penelitianmenunjukkan bahwa ekstrak biji B. asiatica bersifat toksik sedang terhadap S. litura dengan nilai LC50sebesar 0,491% dan berpengaruh terhadap bobot basah larva. Getah A. indica bersifat toksik ringan dengannilai LC50 1,310%. Campuran ekstrak biji B. asiatica dan getah A. indica berdasarkan rasio 4,1% B. asiaticaberbanding 4,9% A. indica memiliki LC50 sebesar 0,970% (toksisitas ringan) dengan LC95 sebesar 15,99% dandinyatakan bersifat antagonis pada LC95 dengan nilai Nisbah Kotoksisitas 0,23% pada 12 hari setelahaplikasi.Kata kunci: Nisbah Kotoksisitas, Kerja bersama bebas, Barringtonia asiatica, Azadirachta indica, Spodopteralitura

scholarly journals Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae)

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
I Made Indra Agastya ◽  
Aminudin Afandhi ◽  
Luqman Qurata Aini
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Megaterium ◽  
Spodoptera Litura ◽  
Laboratory Studies ◽  
Pests And Diseases ◽  
Control Effectiveness ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Lepidoptera Noctuidae

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura

scholarly journals AKTIVITAS EKSTRAK BIJI TANAMAN MINDI MELIA AZEDARACH (L.) TERHADAP SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE)

2005 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Hamdani Hamdani
Keyword(s):  
Biological Activity ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Developmental Time ◽  
Seed Extract ◽  
Melia Azedarach ◽  
The Third ◽  
First Instar ◽  
Lepidoptera Noctuidae

Activity of Melia azedarach (L.) seed extract against armyworm Spodoptera litura (F.) (Lepidoptera: Noctuidae). This study was conducted to evaluate the effectivenes and biological activity of Melia azedarach L. (Meliaceae) seed extract against armyworm, Spodoptera litura F. The first instar larvae were fed extract-treated cotton leaves for 2 days, then were maintained on untreated leaves until the third instar stage. Records were kept in regard to the larvae mortality and developmental time of surviving larvae from first instar to third instar. The result showed that Melia azedarach L. seed extract at consentration of 50 g of seeds/l of water (5%) exhibited moderate insecticidal activity against S. litura larvae (43.33 - 68.33% mortality). Addition of detergen at 0.2% to extract did not increase insecticidal activity of the extract. However, boiling seed extract at consentration of 50 g of seeds/l of water (5%) during 10 until 20 minutes increased insecticidal activity of extract (66.67 - 68.33% mortality). Generally, M. azedarach seed extract treatment did not affect  developmental time of  S. litura larvae.

scholarly journals Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae

2019 ◽  
Vol 1 (1) ◽  
pp. 37
Author(s):  
Raden Arif Malik Ramadhan ◽  
Neneng Sri Widayani ◽  
Lindung Tri Puspasari ◽  
Yusup Hidayat ◽  
Danar Dono
Keyword(s):  
Mortality Rate ◽  
Food Consumption ◽  
Azadirachta Indica ◽  
Dry Weight ◽  
Laboratory Evaluation ◽  
Feeding Method ◽  
Lc50 Value ◽  
And Control

This study aims to evaluate the bioactivity of the formulation of Neem 50 EC  against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of the formulation of neem 50 EC at concentrations of 0.3%, 0.5%, 0.8%, 1.3%, 2.3%, and control. The treatment was done by leaf feeding method and tested on C. pavonana instar II. The results of the research showed that the formulation of neem 50 EC at concentration of 2.3% caused mortality of C. pavonana larvae with mortality rate of 95% and has LC50 value aqual to 0.83%. Neem formulation can inhibit the development of C. pavonana larvae from instar I to instar IV, decrease food consumption, and decrease the dry weight of C. pavonana larvae instar IV.Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation

scholarly journals Bioaktivitas Formulasi Minyak Biji Azadirachta indica (A. Juss) terhadap Spodoptera litura F.

Agrikultura ◽  
2016 ◽  
Vol 27 (1) ◽  
Author(s):  
Raden Arif Malik Ramadhan ◽  
Lindung Tri Puspasari ◽  
Rika Meliansyah ◽  
Rani Maharani ◽  
Yusup Hidayat ◽  
...  
Keyword(s):  
Food Consumption ◽  
Azadirachta Indica ◽  
Spodoptera Litura ◽  
Development Time ◽  
Probit Analysis ◽  
Neem Seed ◽  
Lc50 Value ◽  
Oil Formulation ◽  
Neem Seed Oil ◽  
Test Insect

ABSTRACTBioactivity Formulation of Seed Neem Oil Azadirachta indica (A.Juss) against Spodoptera litura (F)The purpose of this research was to know the influence of neem seed extract formulation (Azadin 50 EC) on the mortality, larvae development, larvae weight, and food consumption of Armyworm (Spodoptera litura). This research used randomized complete design with 6 treatments and 5 replications, i.e. control, formula at concentration of 0.2%, 0.4%; 0.8%; 1.6%; and 3.2%. Correlation of concentration neem seed oil formulation and mortality of test insect was analysed using probit analysis, weight of test larvae presented in mean and standard deviation, and development time and food consumption of test larvae analysed with analysis of varians. Formula Azadin 50 EC had LC50 value of 0.659% (0.550-0.781%) at 12 days after treatment. The mortality increased significantly in pupae stage that caused LC50 value become 0.152%. The formula prolonged development time, decrease the weight of test insect and decrease food consumption by the larvae.Keywords: Lethal concentration, Mortality, Growth derangement, ExtractABSTRAKPenelitian ini bertujuan untuk mempelajari perlakuan formulasi minyak biji mimba Azadin 50 EC terhadap mortalitas, perkembangan larva, bobot larva dan konsumsi pakan ulat grayak (Spodoptera litura F.). Metode Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dengan 5 kali ulangan. Perlakuan tersebut yaitu : Kontrol, konsentrasi formula minyak mimba Azadin 50 EC 0,2%; 0,4%; 0,8%; 1,6% dan 3,2%. Hubungan mortalitas dengan konsentrasi formula dianalisis menggunakan analisis probit, sedangkan data bobot larva, konsumsi pakan dan waktu perkembangan larva dianalisis dengan sidik ragam. Hasil penelitian menunjukkan bahwa formula minyak biji A. Indica memiliki nilai LC50 sebesar 0,659% (0,550-0,781%) terhadap larva instar 2 hingga instar 4. Pada fase pupa kematian kembali meningkat tajam sehingga nilai LC50 menjadi 0,152%. Perlakuan formula tersebut mengakibatkan perpanjangan waktu perkembangan, menurunkan bobot, dan menurunkan konsumsi pakan larva uji.Kata Kunci: Konsentrasi letal, Mortalitas, Gangguan perkembangan, Ekstrak

scholarly journals Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura

2019 ◽  
Vol 1 (1) ◽  
pp. 20
Author(s):  
Hedi Paramita ◽  
Lindung Tri Puspasari ◽  
Yusup Hidayat ◽  
Rika Meliansyah ◽  
Danar Dono ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Development Time ◽  
Feeding Activity ◽  
Seed Extract ◽  
Control Treatment ◽  
Pests And Diseases ◽  
Randomized Design ◽  
Kalanchoe Pinnata ◽  
And Control

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.

scholarly journals PENGARUH FRAKSI AKTIF AGLAIA HARMSIANA PERKINS (MELIACEAE) TERHADAP FISIOLOGI LARVA SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE)

2006 ◽  
Vol 6 (1) ◽  
pp. 1-8
Author(s):  
Eka Candra Lina ◽  
Djoko Prijono ◽  
Dadang Dadang
Keyword(s):  
Growth Rate ◽  
Relative Growth Rate ◽  
Topical Application ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Preparation Method ◽  
Seed Extract ◽  
Active Fraction ◽  
Relative Growth ◽  
Lepidoptera Noctuidae

The effect of active fraction Aglalia harmsiana Perkins (Meliaceae) to physiology of Spodoptera litura (F.) (Lepidoptera : Noctuidae) larvae. This study was conducted to evaluate the physiological interferences in the soybean armyworm Spodoptera litura caused by active fractions of Aglaia harmsiana seed extract.  The activity of the test materials was assessed by dietary preparation and topical application method. Repeated fractionations of A. harmsiana extract by chromatographic methods yielded two active fractions, designated as fraction 2-7 (0.0184%) and 2-8 (0.3773%). The results showed that fraction 2-8 had strong insecticidal activity against S. litura larvae, with LC50 by topical application and dietary preparation were 0.49% and 0.0044%, respectively.  A sublethal treatment with the active fraction (LC25) reduced the relative growth rate, efficiency of conversion of ingested food, and efficiency of conversion of digested food by 52.9%, 42.9%, 49.6% with topical application and 66.7%, 50%, 63.8% with dietary preparation method.  The treatments with that fraction at LC10 and LC25 to the fourth instars reduced the activity of invertase enzyme by 34% and 47%, but increased the activity of trehalase by 6.2% and 12.5% as compared with controls.

PENGARUH PEMBERIAN KONSENTRASI BIOINSEKTISIDA DAUN DAN BIJI MIMBA (Azadirachta indica) TERHADAP KEMATIAN ULAT GRAYAK (Spodoptera litura)

2018 ◽  
Vol 10 (02) ◽  
Author(s):  
P. S. Ajiningrum ◽  
I. A. K. Pramushinta
Keyword(s):  
Spodoptera Litura ◽  
Leaf Extract ◽  
Final Analysis ◽  
Seed Extract ◽  
Mortality Data ◽  
Botanical Insecticide ◽  
Nutritional Content ◽  
Variant Analysis ◽  
The Tropics ◽  
Army Worm

Red spinach (Alternanthera amoena) is one of the vegetable commodities that many people in demand for consumption because of its high nutritional content. Efforts to increase the production of red spinach often experience various obstacles. The main obstacle that limits the productivity of red spinach in the tropics is the number of Plant Disturbing Organisms  in the form of pests and potential diseases that can attack and decrease the production of spinach. One of the pests that often attack spinach is the existence of army worm (Spodoptera litura F.). Therefore, farmers need a way to eradicate the pest. This research is experimental using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with LSD (Least Significant Different) test. Provision of botanical insecticide done by spraying the extract of leaves and seeds mimba with concentration dose 0%, 5%, 10% and 15%. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba leaf extract obtained significant value of 76.263 at a significant level of 0.05 so it can be concluded that there is influence of mimba leaf extract on mortality of army worm. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba seed extract obtained significant value of 78.625 at a significant level of 0.05 so it can be concluded that there is an effect of mimba seeds extract on mortality army worm.   Keywords: mimba leaf extract,  mimba seed extract, red spinach (Alternanthera amoena), army worm (Spodoptera litura F.), botanical insecticide

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