PENGARUH PEMBERIAN KONSENTRASI BIOINSEKTISIDA DAUN DAN BIJI MIMBA (Azadirachta indica) TERHADAP KEMATIAN ULAT GRAYAK (Spodoptera litura)

Author(s):  
P. S. Ajiningrum ◽  
I. A. K. Pramushinta

Red spinach (Alternanthera amoena) is one of the vegetable commodities that many people in demand for consumption because of its high nutritional content. Efforts to increase the production of red spinach often experience various obstacles. The main obstacle that limits the productivity of red spinach in the tropics is the number of Plant Disturbing Organisms  in the form of pests and potential diseases that can attack and decrease the production of spinach. One of the pests that often attack spinach is the existence of army worm (Spodoptera litura F.). Therefore, farmers need a way to eradicate the pest. This research is experimental using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with LSD (Least Significant Different) test. Provision of botanical insecticide done by spraying the extract of leaves and seeds mimba with concentration dose 0%, 5%, 10% and 15%. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba leaf extract obtained significant value of 76.263 at a significant level of 0.05 so it can be concluded that there is influence of mimba leaf extract on mortality of army worm. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba seed extract obtained significant value of 78.625 at a significant level of 0.05 so it can be concluded that there is an effect of mimba seeds extract on mortality army worm.   Keywords: mimba leaf extract,  mimba seed extract, red spinach (Alternanthera amoena), army worm (Spodoptera litura F.), botanical insecticide

2019 ◽  
Vol 4 (1) ◽  
pp. 28
Author(s):  
Kartina Kartina ◽  
Shulkipli Shulkipli ◽  
Mardhiana Mardhiana ◽  
Saat Egra

<h1>Spodoptera litura F. is one of the important pests in Horticulture commodities which can cause damage 80% and up to 100%. Karamunting (Melastome malabathricum) is a wild plant that grows in Tarakan City. This plant is known to contain secondary metabolites which are thought to have the potential to control pests. This study aims to determine the potential of Karamunting extract as a botanical insecticide material to control armyworms. This study uses a completely randomized design (CRD) method with 1 treatment factor, namely the concentration of Karamunting leaf extract. Potential extracts as botanical insecticides were tested by dye method using 4 concentrations, namely 0 (control); 500; 1000; 2000; 4000; and 8000 ppm. The results showed that Karamunting leaf extract was able to control armyworm pests. Giving extracts of 8000 ppm (P5) in feed caused the highest decrease in eating activity up to 41,2%, larval mortality up to 85%, and the highest mortality rate after 7 days of observation. Decreased feeding activity and larval mortality were thought to be influenced by the presence of phenol compounds, fatty acids, terpenoids, sterols and alkaloids identified in the extract. Thus, Karamunting leaf extract has the potential to be developed as an active ingredient in botanical insecticides to control armyworm.<strong></strong></h1>


Jurnal Agro ◽  
10.15575/7409 ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 92-101
Author(s):  
Efrin Firmansyah ◽  
Selvy Isnaeni

Spodoptera litura merupakan serangga polifag yang menyerang banyak komoditas tanaman budidaya. Pengendalian dengan menggunakan insektisida nabati merupakan upaya yang dilakukan untuk meminimalkan dampak negatif penggunaan insektisida kimia sintetik. Tumbuhan S. trilobata diketahui memiliki potensi untuk dijadikan sebagai insektisida nabati untuk mengendalikan S. litura. Aktivitas insektisida tidak hanya ditunjukkan oleh tingkat mortalitas dan penghambatan makan, suatu ekstrak tumbuhan dapat pula memengaruhi pertumbuhan dan perkembangan dari serangga sasaran sehingga dapat dikembangkan menjadi insektisida nabati. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi ekstrak kasar daun S. trilobata terhadap pertumbuhan dan perkembangan larva S. litura. Penelitian terdiri dari tahap pemeliharaan serangga, ekstraksi bagian tumbuhan, dan uji hayati serta analisis fitokimia. Hasil penelitian menunjukkan ekstrak kasar daun S. trilobata yang diaplikasikan memengaruhi pertumbuhan dan perkembangan larva S. litura namun tidak memengaruhi ukuran panjang dan berat pupa yang terbentuk. Hasil analisis fitokimia menunjukkan ekstrak daun S. trilobata mengandung beberapa senyawa metabolit sekunder diantaranya alkaloid, triterpenoid, steroid, flavonoid, tanin dan saponin.                                                                                                    ABSTRACTSpodoptera litura is a polyphagous insect that attacks many commodities of crops. Control using plant-based insecticides is an effort made to minimize the negative effects of using synthetic insecticides. S. trilobata plant is known to have potential as plant-based insecticides to control S. litura. The effectiveness of an insecticide is not only demonstrated by the level of mortality and feeding inhibition activity, a plant extract can also affect the growth and development of the target insect so that it can be developed into a botanical insecticide. The purpose of this study was to determine the effect of S. trilobata leaf crude extract application on growth and development of S. litura larva.  The study stages consisted of insect rearing, extraction of plant parts, bioassay and phytochemical screening. The results showed that S. trilobata leaf extract which was applied affected the growth and development of S. litura larvae but did not affect the length and weight of the pupae formed. The results of phytochemical analysis showed that S. trilobata leaf extract contained several secondary metabolite compounds including alkaloids, triterpenoids, steroids, flavonoids, tannins and saponins.


Author(s):  
Ganiyu Oboh ◽  
Veronica O. Odubanjo ◽  
Fatai Bello ◽  
Ayokunle O. Ademosun ◽  
Sunday I. Oyeleye ◽  
...  

AbstractAvocado pear (The inhibitory effects of extracts on AChE and BChE activities and antioxidant potentials (inhibition of FeThe extracts inhibited AChE and BChE activities and prooxidant-induced TBARS production in a dose-dependent manner, with the seed extract having the highest inhibitory effect and the leaf extract exhibiting higher phenolic content and radical scavenging abilities, but lower Fe chelation ability compared with that of the seed. The phytochemical screening revealed the presence of saponins, alkaloids, and terpenoids in both extracts, whereas the total alkaloid profile was higher in the seed extract than in the leaf extract, as revealed by GC-FID.The anti-cholinesterase and antioxidant activities of avocado leaf and seed could be linked to their phytoconstituents and might be the possible mechanisms underlying their use as a cheap and natural treatment/management of AD. However, these extracts should be further investigated in vivo.


Agrikultura ◽  
2015 ◽  
Vol 26 (1) ◽  
Author(s):  
Indah Meutia Arisanti ◽  
Danar Dono

ABSTRACTBioactivity of mixed seed extract of Barringtonia asiatica l. (kurz.) (lecythidaceae) and sap of Azadirachtaindica A. Juss. (meliaceae) against larvae of Spodoptera litura f. (lepidoptera: noctuidae)Barringtonia asiatica and Azadirachta indica are known to have variety of chemical compounds that haspotency to be developed as botanical pesticides. The study aimed to determine insecticidal toxicity of B.asiatica seed extract and A. indica sap and their mixture. The experiment was carried out in the Laboratoryof Pesticides and Application Technology, Department of Plant Pests and Diseases, Faculty of Agriculture,Universitas Padjadjaran. The experiments were conducted either on toxicity test of the seed extract of B.asiatica and the sap of A. indica singly or their mixture based on the comparison of LC95. Toxicity testing wasperformed using a leaf-residue feeding method on the instar I of Spodoptera litura larvae. The resultsshowed that seed extractof B.asiatica has a moderate toxicity to S.liturawith the LC50 value of 0.491% andaffected body weight of the larvae. The sap of A.indicahas had slightly low toxicity with the LC50 value of1.310%. The mixture of B. asiatica seed extract and A. Indica sap (based on ratio of 4.1%of B. asiaticacompare to 4.9% of A. Indica) has LC50 value of 0.970% (slightly low toxicity) and the LC95 value of 15.99%that was antagonistic on the LC95 level with Cotoxicity Ratio value of 0.23% on 12 days after treatment.Keywords: Cotoxicity ratio, independent joint action, Barringtonia asiatica, Azadirachta indica, SpodopteralituraABSTRAKBarringtonia asiatica dan Azadirachta indica diketahui memiliki berbagai senyawa kimia yang berpotensidikembangkan sebagai pestisida nabati. Penelitian yang bertujuan untuk mengetahui toksisitas ekstrak biji B.asiatica dan getah A. indica serta campurannya telah dilakukan di Laboraturium Pestisida dan TeknikAplikasi, Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran.Penelitian meliputi uji toksisitas ekstrak biji B. asiatica dan getah A.indica secara tunggal, dan pengujiancampuran ekstrak biji B. asiatica dan getah A.indica berdasarkan pada perbandingan LC95. Pengujiantoksisitas dilakukan dengan metode celup pakan pada larva instar I Spodoptera litura. Hasil penelitianmenunjukkan bahwa ekstrak biji B. asiatica bersifat toksik sedang terhadap S. litura dengan nilai LC50sebesar 0,491% dan berpengaruh terhadap bobot basah larva. Getah A. indica bersifat toksik ringan dengannilai LC50 1,310%. Campuran ekstrak biji B. asiatica dan getah A. indica berdasarkan rasio 4,1% B. asiaticaberbanding 4,9% A. indica memiliki LC50 sebesar 0,970% (toksisitas ringan) dengan LC95 sebesar 15,99% dandinyatakan bersifat antagonis pada LC95 dengan nilai Nisbah Kotoksisitas 0,23% pada 12 hari setelahaplikasi.Kata kunci: Nisbah Kotoksisitas, Kerja bersama bebas, Barringtonia asiatica, Azadirachta indica, Spodopteralitura


AGRICA ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 1-8
Author(s):  
Maria Goreti Firma

This study aims to determine the effect of tobacco plant extracts on mortality of armyworm pests (Spodoptera litura F.) and determine the optimum concentration of tobacco leaf extracts on mortality of armyworm caterpillars on mustard plants in the field. The design used in this study was a Randomized Block Design (RBD) consisting of 5 treatments and 4 replications namely T1: 175 ml/L concentration of tobacco leaf extract, T2: 350 ml/L concentration of tobacco leaf extract T3: Concentration of 525 ml/L tobacco leaf extract, T4: Concentration of 700 ml/L tobacco leaf extract, T5: Concentration of 875 ml/L of tobacco leaf extract. The observation variables in this study were pest mortality (%), damage intensity (%), plant fresh weight, and fresh weight per hectare (ton). The results showed that the concentration of tobacco leaf extracts significantly affected the mortality of Spodoptera litura F. At the concentration of T5 treatment (875 ml) were the lowest damage, pest mortality, fresh weight of the mustard plant, and fresh weight per hectare of was 9.75%, 88%, 109.25 gr, and27.31 tons respectively.


2005 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Hamdani Hamdani

Activity of Melia azedarach (L.) seed extract against armyworm Spodoptera litura (F.) (Lepidoptera: Noctuidae). This study was conducted to evaluate the effectivenes and biological activity of Melia azedarach L. (Meliaceae) seed extract against armyworm, Spodoptera litura F. The first instar larvae were fed extract-treated cotton leaves for 2 days, then were maintained on untreated leaves until the third instar stage. Records were kept in regard to the larvae mortality and developmental time of surviving larvae from first instar to third instar. The result showed that Melia azedarach L. seed extract at consentration of 50 g of seeds/l of water (5%) exhibited moderate insecticidal activity against S. litura larvae (43.33 - 68.33% mortality). Addition of detergen at 0.2% to extract did not increase insecticidal activity of the extract. However, boiling seed extract at consentration of 50 g of seeds/l of water (5%) during 10 until 20 minutes increased insecticidal activity of extract (66.67 - 68.33% mortality). Generally, M. azedarach seed extract treatment did not affect  developmental time of  S. litura larvae.


2020 ◽  
Vol 9 (2) ◽  
pp. 15-21
Author(s):  
Ni'matuljannah Akhsan

ABSTRACT The aim of this research is to control environmentally friendly soybean pod borer by utilizing plants in swamps as a vegetable insecticide, namely Kepayang (Pangiumedule). The study was conducted on tidal land in the Village of Kiri Dalam, Barambai District, Barito Kuala Regency, South Kalimantan Province. The study was designed in a randomized block design with 5 replications. The treatment is; 1. Kepayang leaf extract, 2. Without control, 3.Neem plant extract, 4. Chloriliprol insecticide. The results showed that the Kepayang leaf extract can be used as a botanical insecticide controlling soybean pod borer because it can suppress soybean pod attack by up to 62% and is no different from the Chloriliprol insecticide treatment by 64%. Keywords: Applications, Swamp Plants, Boring Pods Borers, Tidal Swamps


PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184639 ◽  
Author(s):  
Farhan Mahmood Shah ◽  
Muhammad Razaq ◽  
Abid Ali ◽  
Peng Han ◽  
Julian Chen

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