scholarly journals STUDY OF BIOCHEMICAL COMPOUNDS AND ANTIMICROBIAL ACTIVITY OF PLEUROTUS INDICA MUSHROOM

2015 ◽  
Vol 2 (2) ◽  
pp. 100-103
Author(s):  
S Pria ◽  
O. N. Shanmugapriya
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Value Added ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Total Carotenoids ◽  
Nutritional Values ◽  
Biochemical Compounds ◽  
Inhibition Zones ◽  
Cultivated Mushrooms

In this study, the nutritional values of dietary mushrooms-Pleurotus indica that are very popular among the cultivated mushrooms in India have been determined. P.indica is widely cultivated as it is rich in nutrients and its component. P.indica is useful in biomass production in commercial level as it contains proteins and minerals. In this study the value added biochemical compounds in P.indica were analyzed from cultures. The amount of total carotenoids, protein, vitamin, pigments and phenolic compounds were examined. P.indica extracts possessed great potential antibacterial activities against four bacterial strains with inhibition zones ranged 8-15mm and MICs values ranges from 25-40É¥g/mL. Thus, it could be suggested that the P.indica is useful biosystem for production of biochemical compounds possess an antimicrobial principles.

scholarly journals The Potential Applications of Bacillus sp. and Pseudomonas sp. Strains with Antimicrobial Activity against Phytopathogens, in Waste Oils and the Bioremediation of Hydrocarbons

Catalysts ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 959 ◽  
Author(s):  
Mariana-Gratiela Soare (Vladu) ◽  
Elena Simina Lakatos ◽  
Nicoleta Ene ◽  
Nereida Malo (Dalanaj) ◽  
Ovidiu Popa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Xanthomonas Campestris ◽  
Selection Process ◽  
Erwinia Carotovora ◽  
Luria Bertani ◽  
Bacterial Strains ◽  
Plant Materials ◽  
Waste Oils ◽  
Potential Applications ◽  
Inhibition Zones

Biodegradation is one of the primary mechanisms for the elimination of petroleum and other hydrocarbon pollutants from the environment. This study presents the results obtained with two newly isolated microorganisms and their potential applications in bioremediation, agriculture, and industrial fields. Twenty-five strains of microorganisms were isolated from plant materials and were subject to a selection process on the basis of antimicrobial activity. Two bacterial strains, respectively Bacillus mycoides (Bm) and Pseudomonas putida (B1), were selected for further experiments, based on the largest inhibition zones against the phytopathogens Erwinia carotovora and Xanthomonas campestris. The production of biosurfactants and enzymes was evaluated in specific media. In order to assess the production of biosurfactants, submerged bioprocesses were carried out on Yeast Malt Peptone Glucose (YMPG), M44, Luria-Bertani (LB), and King B media (KB); the supernatants were used to form emulsions with heptane, octane, and sunflower oil, and the emulsifying indices were determined.

scholarly journals Preparation of Pectin Films from Coffee Cherry and Its Antibacterial Activity

2021 ◽  
Vol 18 (21) ◽  
pp. 34
Author(s):  
Sunita Chamyuang ◽  
Sitthi Duangphet ◽  
Amorn Owatworakit ◽  
Uraiwan Intatha ◽  
Jutamat Nacha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Value Added ◽  
Antibacterial Activities ◽  
Coffee Bean ◽  
Apple Pectin ◽  
Degree Of Esterification ◽  
Coffee Pulp ◽  
Minimum Inhibition Concentration ◽  
Inhibition Concentration

This study aimed to utilize the waste from the coffee bean production process as high value-added material. Pectin extracted from the coffee pulp was classified as low methoxyl pectin (LMP) with the Degree of Esterification (DE) at 33.33 % For further application or upscaling production, the coffee pulp was boiled to retrieve the highest yield at 15.68 g/100 g DW. The antimicrobial assays of crude pectin via minimum inhibition concentration (MIC) showed that coffee pectin could inhibit the growth of Staphylococcus aureus TISTR 1466 in the range of 2.5 - 0.62 mg, while the commercial apple pectin exhibited the inhibition only slightly. The pectin film from the coffee pectin blended with the commercial apple pectin also exhibited antimicrobial activity against S. aureus TISTR 1466. This study shows that coffee pectin could be an alternative material for antibacterial film and other applications. HIGHLIGHTS Pectin extracted from the coffee pulp by this extraction method is classified as low methoxyl pectin with the degree of esterification (DE) at 33.33 % which retrieve the highest yield at 15.68 g/100 g DW of coffee pulp The minimum inhibition concentration (MIC) of the coffee pectin against Staphylococcus aureus TISTR 1466 in the range of 2.5 - 0.62 mg, while the commercial apple pectin did not exhibit antimicrobial activity The pectin film from the coffee pectin 5 % blended with the commercial apple pectin also exhibited antimicrobial activity against aureus TISTR 1466 Given its antibacterial activities, as well as the ability to form film, coffee pectin could be potentially used for application in biomedical or pharmaceutical fields. Importantly, pectin from the coffee pulp could add more value to coffee by-products GRAPHICAL ABSTRACT

scholarly journals Phenolic Composition, Antioxidant and Antimicrobial Activity of Cotoneaster Medik. Species from Bosnia and Herzegovina

2020 ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Acetylcholinesterase Inhibition ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Microbial Strains ◽  
Inhibition Zones ◽  
Antioxidant And Antimicrobial Activity ◽  
Antibacterial And Antifungal ◽  
Ornamental Species

Although the genus Cotoneaster Medik. includes mainly ornamental species, there are some data regarding its biological activity. The purpose of this study was to analyze the content of phenolic compounds, acetylcholinesterase inhibition, antioxidant and antimicrobial activity of methanolic extracts of leaf and bark of C. integerrimus Medik., C. tomentosus (Aiton) Lindl. and C. horizontalis Decne. The C. tomentosus leaf extract exhibited the highest content of total phenols (135.86 mg GAE/g) and flavonoids (18.17 mgQE/g), and also the most potent antioxidant activity against nonbiogenic free radicals, while the highest inhibition of acetylcholinesterase had the leaf extract of C. horizontalis (IC50 0.34 mg/mL). All extracts showed a significant level of antibacterial and antifungal activity against tested microbial strains. The largest inhibition zones were observed against Candida albicans treated with C. integerrimus leaf extract (30.50±0.50 mm). Furthermore, C. integerrimus extract was the most effective in the majority of bacterial strains tested. The results indicated that methanolic extracts of the investigated Cotoneaster species have promising bioactive and therapeutic potentials

Biosynthesis of silver nanoparticles using Bacillus subitilis and its antibacterial activity

2021 ◽  
Vol 30 (2) ◽  
pp. 67-71
Author(s):  
Ghada S.M. Abd el wahab
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Standard Strain ◽  
Physical Chemical ◽  
Inhibition Zones ◽  
Reference Strains ◽  
And Staphylococcus Aureus

Background: Silver nanoparticles (AgNPs) have recently been extensively investigated because of their superior physical, chemical, and biological characteristics, and their superiority is primarily due to the size, shape, composition, crystallinity, and structure of AgNPs compared to their bulk forms. Objective: The current study aimed to investigate the antibacterial activity of silver nanoparticles (AgNPs) synthesized intracellular by using standard strain Bacillus subitilis ATCC 6633 against reference strains Escherichia coli ATCC 2592 and Staphylococcus aureus ATCC 29737. Results: The synthesized AgNPs showed potent antibacterial activities against the two tested bacterial strains with inhibition zones ranged from 42 -52mm and MIC 27.2 µg / ml. The silver nanoparticles were characterized with particle size ≃ 100 nm and zeta potential -19. There was deformation in both tested strains upon treatment with AgNPs which was observed by Scanning Electron Microscopy (SEM). Conclusion: The results indicated that AgNPs could be used as an effective antibacterial agent.

scholarly journals A PRELIMINARY OBSERVATION ON AN EXPLICIT ANTIMICROBIAL ACTION OF MANGROVE PLANTS ON PSEUDOMONAS AERUGINOSA

2019 ◽  
pp. 226-230
Author(s):  
TAMANNA SULTANA ◽  
ARUP KUMAR MITRA ◽  
SATADAL DAS
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Microtiter Plate ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Mangrove Plant ◽  
E Coli ◽  
Mangrove Plants

Objectives: Due to emerging drug-resistant microorganisms throughout the world, newer antimicrobial agents should be looked for. Plants are enriched with different bioactive chemicals. In this study, we searched antibacterial activities of some mangrove plant extracts against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Methods: In the present study, the antimicrobial activity of the leaves of Bruguiera gymnorhiza, Excoecaria agallocha, Avicennia alba, and Aegialitis rotundifolia was evaluated against a few reference pathogenic bacterial strains, namely, P. aeruginosa ATCC 27853, E. coli ATCC 25922, S. aureus ATCC 29213, and multidrug-resistant bacterial strains E. coli extended-spectrum beta-lactamases strain. Aqueous, ethanolic, methanolic, and dimethyl sulfoxide (DMSO) extracts were studied. The antimicrobial activities of the organic solvent extracts on the various test microorganisms were investigated using agar well diffusion technique followed by determination of minimum inhibitory concentration values by serial dilution in a microtiter plate. Results: Ethanol and DMSO extracts of B. gymnorhiza exhibited promising antimicrobial activity followed by extracts of A. alba and E. agallocha. Among all microorganisms studied, P. aeruginosa ATCC 27853 showed significant growth inhibition with ethanol and DMSO extracts. Conclusion: Extracts of some mangrove plants, particularly, B. gymnorhiza showed very good antimicrobial activities against common microbial agents causing human infections and in general mangrove plants appear to act better on P. aeruginosa.

scholarly journals Phytochemical Profiles, Antioxidant and Antibacterial Activities of Grape (Vitis vinifera L.) Seeds and Skin from Organic and Conventional Vineyards

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1470 ◽  
Author(s):  
Cristiana Radulescu ◽  
Lavinia Claudia Buruleanu ◽  
Cristina Mihaela Nicolescu ◽  
Radu Lucian Olteanu ◽  
Marius Bumbac ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Vitis Vinifera L ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Therapeutic Benefits ◽  
Inhibition Concentration ◽  
Red Grape

The therapeutic benefits of extracts obtained from different red grape fractions were thoroughly studied, however, data regarding the comparison of phytochemical extracts prepared from the same varieties coming from organic versus conventional management systems are rather lacking. The present study aimed at comparing some of the phytochemical characteristics and antimicrobial activity of hydroalcoholic (50% v/v) extracts obtained from four varieties of red grapes cultivated respectively in organic and conventional vineyards. Total flavonoid content, total phenolic compounds, and antioxidant activity were determined by molecular absorption spectroscopy. Antimicrobial activity of the studied extracts was evaluated against common bacterial strains isolated from different habitats according to specific lab procedures. The analyses were performed in solid broths by applying the disk diffusion method, which allowed for the simultaneous determination of the spectrum of the sensitivity of the tested bacteria as well as the values of the minimum inhibition concentration (MIC). It was found that favorable antagonistic activities against the tested bacteria strains were exhibited by the hydroalcoholic extracts from the seeds of the organic varieties, respectively the skin of the conventional varieties.

scholarly journals Chemical Characterisation and Biological Effects of Essential Oils of Four Gabonese Medicinal Plants

2021 ◽  
pp. 52-60
Author(s):  
Elvis Jolinom Mbot ◽  
Cédric Sima Obiang ◽  
Maximilienne Ascenssion Nyegue ◽  
Bill Raphaël Bikanga ◽  
Huguette Agnaniet ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oils ◽  
Biological Effects ◽  
Antibacterial Activities ◽  
Chemical Compositions ◽  
Aromatic Plants ◽  
Bacterial Strains

Aims: The objective of this work is to determine the chemical composition and in vitro evaluation of the antibacterial activities of essential oils of four species of aromatic plants of Gabonese origin obtained by hydrodistillation. Methods: All the samples were examined by chromatographic analyzes and by GC / MS coupling. Antimicrobial activity was assessed by diffusion and microdilution methods. Results: The most common compounds found in essential oils were terpene hydrocarbons and oxygenates. The major constituents are β-phellandrene (56.3%), β-pinene (11%) and myrcene (10.4%). The three bacterial strains used are sensitive to essential oils. However, some essential oils stood out with greater spectra of action compared to others, such as Maranthes gabunensis and Mammea africana which showed activity against all strains. The inhibition parameters (MIC and CMB) are between 0.78 and 25 mg / mL. Some oils have shown bacteriostatic and / or bactericidal activity on the targeted strains. Conclusion: The essential oils studied present a diversity of chemical compositions and good antibacterial activities.

scholarly journals Effects of Natural Antimicrobials on Xanthomonas Strains Growth

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Irena Mačionienė ◽  
Dovilė Čepukoit ◽  
Joana Šalomskienė ◽  
Darius Černauskas ◽  
Daiva Burokienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Lactobacillus Helveticus ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Natural Antimicrobials ◽  
Inhibition Zones

The aim of this work was to investigate the most promising natural antimicrobials effective for the growth suppression of Xanthomonas spp. bacteria. The research objects were Xanthomonas spp. strains isolated from tubers and stem of plants growing in Lithuania: Xanthomonas translucens NRCIB X6, X. arboricola NRCIB X7, NRCIB X8, NRCIB X9, and NRCIB X10; the supernatants of lactic acid bacteria Lactococcus lactis strains 140/2, 57, and 768/5, Lactobacillus helveticus strains 14, 148/3, R, and 3, Lb. reuteri 3 and 7, Streptococcus thermophilus 43, Enterococcus faecium 59-30 and 41-2; endophytic bacterial strains Bacillus, Pseudomonas, and Paenibacillus spp.; and essential oils of lavender (Lavandula angustifolia), grapefruit (Citrus paradisi), pine (Pinus sylvestris), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), peppermint (Mentha piperita), lemon (Citrus limetta), aqueous extracts of blueberries (Vaccinium myrtillus), and cranberries (Vaccinium vitis-idaea). The antimicrobial activity of tested substances was determined by agar diffusion method. Supernatants of Lb. reuteri strain 7 and Lb. helveticus strains 14, R, 3, and 148/3 were found to have a high antimicrobial activity against Xanthomonas spp. bacteria strains when compared to the positive control—1.0% copper sulfate (diameter of inhibition zones was 28.8 ± 0.7 mm). The diameter of inhibition zones of supernatants ranged from 23.3 ± 0.6 mm to 32.0 ± 0.1 mm. Thyme (2.0%) and lavender (2.0%) essential oils inhibited the growth of Xanthomonas spp. strains. The diameter of the inhibition zones was from 14.7 ± 0.8 mm to 22.8 ± 0.9 mm. The aqueous extracts of blueberries had a weak antimicrobial activity. The diameter of inhibition zones ranged from 11.0 ± 0.2 mm to 13.0 ± 0.2 mm.

scholarly journals Antimicrobial Activity of Chloroform and Methanol Extract of Lennea coromandelica Merr

2015 ◽  
Vol 45 ◽  
pp. 67-74 ◽  
Author(s):  
P. Vedhanarayanan ◽  
T. Vaithiyanathan ◽  
P. Sundaramoorthy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition ◽  
Inhibition Zones ◽  
Bacterial Concentrations

The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus,Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.

Antimicrobial Activity of Extracts of Edible Wild and Cultivated Mushrooms against Foodborne Bacterial Strains

2008 ◽  
Vol 71 (8) ◽  
pp. 1701-1706 ◽  
Author(s):  
M. E. VENTURINI ◽  
C. S. RIVERA ◽  
C. GONZALEZ ◽  
D. BLANCO
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Salmonella Enteritidis ◽  
Lentinula Edodes ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Disk Diffusion Assay ◽  
Hexane Extracts ◽  
Cultivated Mushrooms

The antimicrobial activity of aqueous, methanol, hexane, and ethyl acetate extracts from edible wild and cultivated mushrooms against nine foodborne pathogenic bacterial strains (Escherichia coli O157:H7, Salmonella Enteritidis, Shigella sonnei, Vibrio parahaemolyticus, Yersinia enterocolitica, Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, and Staphylococcus aureus) was screened with a disk diffusion assay. Twenty-nine of the 48 species tested had antimicrobial activity. Methanol, ethyl acetate, and aqueous extracts accounted for 92.8% of the positive assays, whereas the hexane extracts accounted for only 7.2%. Gram-positive bacteria were more sensitive than gram-negative bacteria to fungal extracts, and C. perfringens was the most sensitive microorganism. Aqueous extracts from Clitocybe geotropa and Lentinula edodes had the highest antimicrobial activity against all the bacterial strains tested.

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