scholarly journals Phenolic Composition, Antioxidant and Antimicrobial Activity of Cotoneaster Medik. Species from Bosnia and Herzegovina

2020 ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Acetylcholinesterase Inhibition ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Microbial Strains ◽  
Inhibition Zones ◽  
Antioxidant And Antimicrobial Activity ◽  
Antibacterial And Antifungal ◽  
Ornamental Species

Although the genus Cotoneaster Medik. includes mainly ornamental species, there are some data regarding its biological activity. The purpose of this study was to analyze the content of phenolic compounds, acetylcholinesterase inhibition, antioxidant and antimicrobial activity of methanolic extracts of leaf and bark of C. integerrimus Medik., C. tomentosus (Aiton) Lindl. and C. horizontalis Decne. The C. tomentosus leaf extract exhibited the highest content of total phenols (135.86 mg GAE/g) and flavonoids (18.17 mgQE/g), and also the most potent antioxidant activity against nonbiogenic free radicals, while the highest inhibition of acetylcholinesterase had the leaf extract of C. horizontalis (IC50 0.34 mg/mL). All extracts showed a significant level of antibacterial and antifungal activity against tested microbial strains. The largest inhibition zones were observed against Candida albicans treated with C. integerrimus leaf extract (30.50±0.50 mm). Furthermore, C. integerrimus extract was the most effective in the majority of bacterial strains tested. The results indicated that methanolic extracts of the investigated Cotoneaster species have promising bioactive and therapeutic potentials

scholarly journals COMPARISON OF ANTIMICROBIAL EFFICACY OF THREE TYPES OF MOUTHWASH, CONTAINING CHLORHEXIDINE-CHLORBUTANOL, ALCOHOL-ESSENTIAL OILS AND PROPOLIS-MENTHA OIL COMBINATIONS

2020 ◽  
Vol 26 (4) ◽  
pp. 3398-3402
Author(s):  
Neli Ermenlieva ◽  
◽  
Emilia Georgieva ◽  
Minko Milev ◽  
Nadya Agova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Antiseptic Solution ◽  
Agar Diffusion ◽  
Microbial Strains ◽  
Inhibition Zones ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Mouthwash is an antiseptic solution intended to reduce the microbial load in the oral cavity, although other mouthwash might be given for other reasons such as for their analgesic, anti-inflammatory or anti-fungal action. A study was carried out to compare the antibacterial and antifungal properties of three mouthrinse preparations - both containing commercial used antiseptic combinations (chlorhexidine 0,100% + chlorbutanol 0,500% and alcohol 21,60% + essential oils) and one with natural active ingredients (propolis 2,00% + mentha oil 0, 042%). The antibacterial and antifungal activity of three types of mouthwash were tested on three microbial strains - Staphylococcus aureus, Escherichia coli and Candida albicans by two alternatives of agar diffusion tests – “cup plate” technique and disc-diffusion test. The results showed the highest antimicrobial activity of the chlorhexidine-chlorbutanol combination. Mouthwash containing propolis with mint oil demonstrated activity only against S. aureus strain. It turned out that testing the antimicrobial activity of mouthwash with alcohol with essential oils with the agar diffusion method is not representative. For 24 hours with this mouthwash, no inhibition zones were observed in none of the strains at any concentration. Our assumption is that alcoholic solutions are exuding and bacteria starts to grow.

scholarly journals Phytochemical investigation and in vitro antimicrobial activity of Richardia scabra

2016 ◽  
Vol 11 (2) ◽  
pp. 248 ◽  
Author(s):  
Kathirvel Poonkodi ◽  
Subban Ravi
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Weed Species ◽  
Antibacterial And Antifungal Activities ◽  
Agar Plug ◽  
Phytochemical Investigation ◽  
Antibacterial And Antifungal ◽  
Simple Sugar

<p class="Abstract">The present study was aimed to evaluate the phytochemical screening and antimicrobial activity of the petroleum ether and methanol extracts from the mature leaves of <em>Richardia scabra</em> from India. Disc diffusion method was used to determine the zone inhibition of the tested samples for antibacterial and agar plug method was used to determine the antifungal activity, while the microtube-dilution technique was used to determine the minimum inhibitory concentration. Both extracts showed significant antibacterial and antifungal activities when tested against 10 bacterial and four fungal strains. The minimum inhibitory concentrations of the methanol extract of<em> R. scabra</em> ranged between 12.5–100 μg/mL for bacterial strains. Alkaloids, steroids, flavonoids, fatty acids, terpenoids and simple sugar were detected as phytoconstituents of extracts. To the best of our knowledge, this is the first report against antimicrobial activity of common weed species <em>R. scabra</em> found in India.</p><p> </p>

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Pharmacognostical, Phytochemical, Antioxidant and Antimicrobial Activity of Costus woodsonii

2019 ◽  
pp. 53-59
Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Concentration Test ◽  
Antioxidant And Antimicrobial Activity

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.

scholarly journals Synthesis of Cinnamanilide Derivatives and Their Antioxidant and Antimicrobial Activity

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Satish Balasaheb Nimse ◽  
Dilipkumar Pal ◽  
Avijit Mazumder ◽  
Rupa Mazumder
Keyword(s):  
Antimicrobial Activity ◽  
Cinnamic Acid ◽  
Antioxidant Activities ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Strong Activity ◽  
Amide Derivatives ◽  
Antioxidant And Antimicrobial Activity ◽  
Derivatives Of ◽  
Strong Antimicrobial Activity

The amide derivatives of cinnamic acid were synthesized and their antimicrobial and antioxidant activities were investigated. The investigation of antimicrobial potentials of the compounds demonstrated a strong activity against 21 bacterial strains comprising Gram-positive and Gram-negative bacteria. Compounds2a,2b, and3bshowed strong antimicrobial activity against all microorganisms with the pMIC value ranging from 2.45 to 3.68. Compounds2a,3a, and3bdemonstrated strong antioxidant activity with % inhibition of the DPPH radical of 51% (±1.14), 41% (±1.01), and 50% (±1.23), respectively. These findings indicate that the amide derivatives of the cinnamic acid possess strong antibacterial and antioxidant activities.

An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers

2008 ◽  
Vol 9 (5) ◽  
pp. 60-67
Author(s):  
Leena Smadi ◽  
Azmi Mahafzah ◽  
Ameen Khraisat
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Bacterial Strains ◽  
A Value ◽  
Agar Diffusion Test ◽  
Ah Plus ◽  
Microbial Strains ◽  
And Control ◽  
Root Canal Sealers

Abstract Aim The purpose of this in vitro study was to analyze the antimicrobial activity of root canal sealers by using the agar diffusion test (ADT). Methods and Materials Three categories of root canal sealers were included in the study: resin-based sealers (4), zinc oxide-based sealers (3), and calcium hydroxide-based sealers (2). The microbial strains used were: S. aureus (2 strains), C. albicans (2 strains), and E. faecalis (1 strain). Statistical analysis was conducted using a one-way analysis of variance (ANOVA). Tests of differences were analyzed using the Tukey's test with a value of p < 0.05 considered statistically significant. Results The antimicrobial activity of root canal sealers was ranked in descending order as follows: Sealite Regular, Cortisemol, Dentalis KEZ, AH26, Sealapex, Acroseal/Topseal, and Endorez/AH plus. Conclusion Root canal sealers showed different inhibitory effects depending on their types and the bacterial strains tested. Root canal sealers containing formaldehyde and eugenol proved to be effective against the microorganisms studied. Clinical Significance The incorporation of antimicrobial components into root canal sealers may become an essential factor in preventing the re-growth of residual bacteria and control of bacteria re-entry into the root canal system. Citation Smadi L, Mahafzah A, Khraisat A. An In vitro Evaluation of the Antimicrobial Activity of Nine Root Canal Sealers. J Contemp Dent Pract 2008 July; (9)5:060-067.

scholarly journals Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

2010 ◽  
Vol 75 (6) ◽  
pp. 739-747 ◽  
Author(s):  
Dinesh Bisht ◽  
Rajendra Padalia ◽  
Lalit Singh ◽  
Veena Pande ◽  
Priyanka Lal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antagonistic Activity ◽  
Significant Activity ◽  
Variable Degree ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

scholarly journals Antimicrobial Activity ofLippiaSpecies from the Brazilian Semiarid Region Traditionally Used as Antiseptic and Anti-Infective Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Cristiana da Purificação Pinto ◽  
Velize Dias Rodrigues ◽  
Fernanda da Purificação Pinto ◽  
Renata da Purificação Pinto ◽  
Ana Paula Trovatti Uetanabaro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Infections ◽  
Active Species ◽  
Semiarid Region ◽  
Antimicrobial Drugs ◽  
Promising Alternative ◽  
Popular Medicine ◽  
Antibacterial And Antifungal Activities ◽  
Methanolic Extracts ◽  
Antibacterial And Antifungal

Lippia origanoidesKunth,Lippia alnifoliaSchauer, andLippia thymoidesMartius and Schauer are shrubs used in the traditional Brazilian medicine as antiseptics, as well as in the treatment of infectious diseases. This study was designed to investigate the antibacterial and antifungal activities of the methanolic extracts of these species, as new potential sources of antimicrobial drugs. The antimicrobial activity of methanolic extracts was investigated against resistant yeasts and bacteria by agar disk diffusion. Then, the MIC determination of the most active species and its fractions in hexane, dichloromethane, ethyl acetate, and water was performed. By the agar diffusion assay, all species were active against at least two microorganisms, giving evidence to support their use in the popular medicine.L. origanoidesleaves exhibited the widest antimicrobial action, inhibiting the growth of two Gram-positive bacteria and two yeasts; this activity was also confirmed by the MIC evaluation. The fractionation ofL. origanoidescrude extracts improved the activity in spectrum and intensity. The results obtained in this study indicate thatL. origanoidesmay be a promising alternative in the treatment of bacterial and fungal infections and in the seeking of new antimicrobial drugs.

scholarly journals The Potential Applications of Bacillus sp. and Pseudomonas sp. Strains with Antimicrobial Activity against Phytopathogens, in Waste Oils and the Bioremediation of Hydrocarbons

Catalysts ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 959 ◽  
Author(s):  
Mariana-Gratiela Soare (Vladu) ◽  
Elena Simina Lakatos ◽  
Nicoleta Ene ◽  
Nereida Malo (Dalanaj) ◽  
Ovidiu Popa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Xanthomonas Campestris ◽  
Selection Process ◽  
Erwinia Carotovora ◽  
Luria Bertani ◽  
Bacterial Strains ◽  
Plant Materials ◽  
Waste Oils ◽  
Potential Applications ◽  
Inhibition Zones

Biodegradation is one of the primary mechanisms for the elimination of petroleum and other hydrocarbon pollutants from the environment. This study presents the results obtained with two newly isolated microorganisms and their potential applications in bioremediation, agriculture, and industrial fields. Twenty-five strains of microorganisms were isolated from plant materials and were subject to a selection process on the basis of antimicrobial activity. Two bacterial strains, respectively Bacillus mycoides (Bm) and Pseudomonas putida (B1), were selected for further experiments, based on the largest inhibition zones against the phytopathogens Erwinia carotovora and Xanthomonas campestris. The production of biosurfactants and enzymes was evaluated in specific media. In order to assess the production of biosurfactants, submerged bioprocesses were carried out on Yeast Malt Peptone Glucose (YMPG), M44, Luria-Bertani (LB), and King B media (KB); the supernatants were used to form emulsions with heptane, octane, and sunflower oil, and the emulsifying indices were determined.

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