scholarly journals Preparation of Pectin Films from Coffee Cherry and Its Antibacterial Activity

2021 ◽  
Vol 18 (21) ◽  
pp. 34
Author(s):  
Sunita Chamyuang ◽  
Sitthi Duangphet ◽  
Amorn Owatworakit ◽  
Uraiwan Intatha ◽  
Jutamat Nacha ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Value Added ◽  
Antibacterial Activities ◽  
Coffee Bean ◽  
Apple Pectin ◽  
Degree Of Esterification ◽  
Coffee Pulp ◽  
Minimum Inhibition Concentration ◽  
Inhibition Concentration

This study aimed to utilize the waste from the coffee bean production process as high value-added material. Pectin extracted from the coffee pulp was classified as low methoxyl pectin (LMP) with the Degree of Esterification (DE) at 33.33 % For further application or upscaling production, the coffee pulp was boiled to retrieve the highest yield at 15.68 g/100 g DW. The antimicrobial assays of crude pectin via minimum inhibition concentration (MIC) showed that coffee pectin could inhibit the growth of Staphylococcus aureus TISTR 1466 in the range of 2.5 - 0.62 mg, while the commercial apple pectin exhibited the inhibition only slightly. The pectin film from the coffee pectin blended with the commercial apple pectin also exhibited antimicrobial activity against S. aureus TISTR 1466. This study shows that coffee pectin could be an alternative material for antibacterial film and other applications. HIGHLIGHTS Pectin extracted from the coffee pulp by this extraction method is classified as low methoxyl pectin with the degree of esterification (DE) at 33.33 % which retrieve the highest yield at 15.68 g/100 g DW of coffee pulp The minimum inhibition concentration (MIC) of the coffee pectin against Staphylococcus aureus TISTR 1466 in the range of 2.5 - 0.62 mg, while the commercial apple pectin did not exhibit antimicrobial activity The pectin film from the coffee pectin 5 % blended with the commercial apple pectin also exhibited antimicrobial activity against aureus TISTR 1466 Given its antibacterial activities, as well as the ability to form film, coffee pectin could be potentially used for application in biomedical or pharmaceutical fields. Importantly, pectin from the coffee pulp could add more value to coffee by-products GRAPHICAL ABSTRACT

Synthesis and antimicrobial activity of 4-trifluoromethylpyridine nucleosides

2017 ◽  
Vol 23 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Shaikha S. Alneyadi ◽  
Anas A. Abdulqader ◽  
Alaa A. Salem ◽  
Ibrahim M. Abdou
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Stenotrophomonas Maltophilia ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Nucleoside Analogues ◽  
Reference Drug ◽  
Minimum Inhibitory Concentrations

Abstract4-Trifluoromethylpyridine derivatives 4–8 represent good candidates for the discovery of new antibacterial agents. Fluorinated pyridine nucleosides 4–7 and non-nucleoside analogues 8a,b were synthesized and evaluated for their antibacterial activities against Staphylococcus aureus, Bacillus infantis, Escherichia coli and Stenotrophomonas maltophilia. The minimum inhibitory concentrations (MICs) of the new nucleosides 4–7 range from 1.3 to 4.9 μg/mL and MICs of fluoroaryl derivatives 8a,b are in the range of 1.8–5.5 μg/mL. Activity of amoxicillin, the reference drug, is 1.0–2.0 μg/mL under similar conditions.

scholarly journals Ultrastructural Study of Elaeis guineensis (Oil Palm) Leaf and Antimicrobial Activity of its Methanol Extract against Staphylococcus Aureus

2016 ◽  
Vol 12 (3) ◽  
pp. 419-423 ◽  
Author(s):  
Amala Rajoo ◽  
Sreenivasan Sasidharan ◽  
Subramanion L Jothy ◽  
Surash Ramanathan ◽  
Sharif M Mansor
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Transmission Electron Microscopy ◽  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Elaeis Guineensis ◽  
Transmission Electron ◽  
Inhibition Concentration ◽  
Scanning Electron

Purpose: To evaluate the antimicrobial activity of the methanol extract of Elaeis guineensis leaf against Staphylococcus aureus and to determine the effect of extract treatment on the microstructure of the microbeMethods: The antimicrobial activity of the methanol leaf extract of the plant against S. aureus was examined using disc diffusion and broth dilution methods. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were carried out to determine the major alterations in the microstructure of S. aureus after treatment with the extract.Results: The extract showed a good antimicrobial activity against S. aureus with a minimum inhibition concentration (MIC) of 6.25 mg/mL and for Chloramphenicol was 30.00 ìg/mL. The main changes observed under SEM and TEM were structural disorganization of the cell membrane which occurred after 12 h and total collapse of the cell 36 h after exposure to the extract.Conclusion: We concluded that the methanolic extract of E. guineensis leaf exhibited good antimicrobial activity against S. aureus and this is supported by SEM and TEM.Keywords: Antimicrobial activity, Elaeis guineensis, Staphylococcus aureus, Scanning electron microscopy, Transmission electron microscopy

scholarly journals Antioxidant and Antibacterial Activities of the Essential Oil of Moroccan Tetraclinis articulata (Vahl) Masters

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Halima Rabib ◽  
Chaimaa Elagdi ◽  
Mohammed Hsaine ◽  
Hassan Fougrach ◽  
Tayeb Koussa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Bornyl Acetate ◽  
Radical Trap ◽  
Tetraclinis Articulata

The purpose of this study is to evaluate and compare the antioxidant and antibacterial activities of essential oil isolated from Tetraclinis articulata (Vahl) leaves, Masters originating in Morocco (Benslimane Region, Atlantic-influenced plain). The analysis of the major compounds of essential oil was performed by gas chromatography and mass spectrometry, and this oil is dominated by bornyl acetate (35.05%), camphor (11.17%), and α-pinene (10.84%). The antioxidant properties were evaluated by the test of the radical trap 2,2-diphényl-1-picrylhydrazyl (DPPH), and the antimicrobial activity of T. articulata essential oil was tested against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli which have been inhibited from the 25 μg/mL.

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

2003 ◽  
Vol 9 (1) ◽  
pp. 49-51
Author(s):  
Farida Lanawati Darsono ◽  
Stephanie Devi Artemisia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Tween 80 ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Gram Positive Bacteria ◽  
Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

scholarly journals THE ANTIBACTERIAL EFFICACY OF ETHANOL EXTRACTS OBTAINED FROM LEAVES OF SOME THYMUS SPECIES (LAMIACEAE) AGAINST STAPHYLOCOCCUS AUREUS SUBSP. AUREUS STRAIN

2021 ◽  
pp. 18-29
Author(s):  
N. Kurhaluk ◽  
H. Tkachenko ◽  
V. Honcharenko ◽  
V. Nachychko ◽  
A. Prokopiv ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activities ◽  
Aureus Strain ◽  
Future Research ◽  
Antibacterial Efficacy ◽  
Disk Diffusion Assay ◽  
The Difference ◽  
Plant Families ◽  
Ethanol Extracts

A convincing number of investigations that indicate that thymol with other metabolites exhibited potent antimicrobial, antifungal, antibacterial, and antiparasitic properties prompted us to verify the antibacterial efficacy of four species and one interspecific hybrid of the Thymus genus collected in the western part of Ukraine against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Considering the points highlighted above and based on previous results obtained in our laboratory, in the current study, we decided to evaluate the antimicrobial efficacy of five ethanol extracts obtained from leaves of Thymus representatives against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. Fresh leaves were washed, weighed, crushed, and homogenized in 96 % ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. Antimicrobial activity was determined using the agar disk diffusion assay. The ethanol extracts obtained from leaves of Thymus plants showed different antibacterial activities against Staphylococcus aureus subsp. aureus (ATCC® 29213™) strain. The antibacterial activity of extracts was greatest for Th. serpyllum followed by Th. pannonicus followed by Th. pulegioides, Th. alpestris, and then by Th. x porcii. Since the antibacterial effectiveness of medicinal plants varies dramatically depending on the phytochemical characteristics of plant families and subfamilies, it is not surprising to note the difference in this efficacy even when using samples taken from the same plant, but from two different regions. The antimicrobial activity of the crude ethanol extracts obtained from leaves of Thymus plants may be attributed to specific compounds or a combination of compounds. The present study lays the basis for future research, to validate the possible use of Thymus species as a candidate in the treatment of infections caused by Staphylococcus aureusin medicine and veterinary.

scholarly journals Cytotoxicity and antimicrobial activity of Dialium ovoideum thwaites, an endemic plant in Sri Lanka

2020 ◽  
Vol 6 (2) ◽  
pp. 316-320
Author(s):  
Vajira P Bulugahapitiya ◽  
Rathnaweera TN ◽  
Wijayaratne WMDGB ◽  
Manawadu HC
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Sri Lanka ◽  
Value Added ◽  
Endemic Plant ◽  
E Coli ◽  
Methanolic Extracts ◽  
Test Organisms ◽  
Agar Well Diffusion Assay

This study was aimed exploring the cytotoxicity and antimicrobial activity of the leaves D. thwaites is an endemic plant to Sri Lanka. The plant is known for its nutritional and medicinal applications where especially the leaf decoctions are being used to wash skin wounds in indigenous medicine. The brine shrimp lethality assays was performed to evaluate normal toxicity and it gave LC50 value greater than 1000 μg/ mL showing that the plant extracts are non-toxic to the normal cells. The agar-well diffusion assay was performed to assess the antimicrobial activity, and strains of bacteria; E. coli, Staphylococcus aureus, Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus (MRSA) and fungus; Candida albicans were used as test organisms. The results indicated that all the extracts are active against Staphylococcus aureus (Gram Positive) with maximum inhibition shown for methanolic and aqueous extracts. When it was tested against MRSA both aqueous and methanolic extracts gave similar inhibitions. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as 6.25 mg/mL and 100 mg/mL respectively with S. aureus whereas no inhibition observed by any of the extract against Gram negative bacteria and Candida albicans. Thus, this study revealed the leaves of D. thwaites possesses strong anti-bacterial activity against S. aureus and MRSA. The results confirmed the efficacy of using D. thwaites as the natural antimicrobial agent and suggested to develop the leaves into value added products to be used in topical applications as antiseptic solutions or ointments. Asian J. Med. Biol. Res. June 2020, 6(2): 316-320

scholarly journals Oxidative Stress and Antimicrobial Activity of Chromium(III) and Ruthenium(II) Complexes onStaphylococcus aureusandEscherichia coli

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina L. Páez ◽  
Claudia M. Bazán ◽  
María E. Bongiovanni ◽  
Judith Toneatto ◽  
Inés Albesa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Diimine Ligands

The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat previously easily treatable infections. The main aim of this work was to establish the potential of the syntheticα-diimine chromium(III) and ruthenium(II) complexes (where theα-diimine ligands are bpy = 2,2-bipyridine, phen = 1,10-phenanthroline, and dppz = dipyrido[3,2-a:2′,3′-c]-phenazine) like [Cr(phen)3]3+, [Cr(phen)2(dppz)]3+, [Ru(phen)3]2+, and [Ru(bpy)3]2+as antibacterial agents by generating oxidative stress. The [Cr(phen)3]3+and [Cr(phen)2(dppz)]3+complexes showed activity against Gram positive and Gram negative bacteria with minimum inhibitory concentrations (MICs) ranging from 0.125 μg/mL to 1 μg/mL, while [Ru(phen)3]2+and [Ru(bpy)3]2+do not exhibit antimicrobial activity against the two bacterial genera studied at the concentration range used. When ciprofloxacin was combined with [Cr(phen)3]3+for the inhibition ofStaphylococcus aureusandEscherichia coli, an important synergistic effect was observed, FIC 0.066 forS. aureusand FIC 0.064 forE. coli. The work described here shows that chromium(III) complexes are bactericidal forS. aureusandE. coli. Our results indicate thatα-diimine chromium(III) complexes may be interesting to open new paths for metallodrug chemotherapy against different bacterial genera since some of these complexes have been found to exhibit remarkable antibacterial activities.

scholarly journals Comparative Antibacterial Efficacy of Vitellaria paradoxa (Shea Butter Tree) Extracts Against Some Clinical Bacterial Isolates

2015 ◽  
Vol 7 (3) ◽  
pp. 264-268 ◽  
Author(s):  
Kamoldeen Abiodun AJIJOLAKEWU ◽  
Fola Jose AWARUN
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Pathogens ◽  
Stem Bark ◽  
Antibacterial Activities ◽  
Shea Butter ◽  
Vitellaria Paradoxa ◽  
Ethanolic Extracts

The antibacterial activities of the ethanolic extracts of seed, leaf and stem bark of Vitellaria paradoxa were investigated. The extracts were tested against three clinical bacterial pathogens, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae using the agar diffusion and the broth dilution techniques. Ethanolic extracts of the plant parts showed activity against all the bacterial pathogens tested. At the highest extract concentration (200 mg/ml), the leaf extract exhibited the highest antimicrobial activity, while no activity was detected at the lowest concentration (3.13 mg/ml) against the tested isolates. Escherichia coli and Staphylococcus aureus were more susceptible to all extracts of V. paradoxa, while Klebsiella pneumoniae showed the least sensitivity. The efficacy of ethanolic extracts of Vitellaria paradoxa was compared to a commercial antibiotic streptomycin. There were differences in the minimum inhibitory concentration (MIC) of all the Vitellaria paradoxa ethanolic extracts with respect to the type of organism. All extracts exhibited bacteriostatic effects against the tested organisms at the experimented concentrations. Qualitative phytochemical screening of the extracts revealed the presence of saponins, tannins and alkaloids as the active principles of Vitellaria paradoxa's antimicrobial activity. V. paradoxa could be used as a potential source of antibiotic substance for a drug development.

scholarly journals Design, Synthesis and Antibacterial Activities of Triazole-pyrimidine Derivatives as SecA Inhibitors

2021 ◽  
Author(s):  
FANTE BAMBA ◽  
Tchambaga Camara ◽  
Coulibali Sioménan ◽  
Sagne Jacques Akpa ◽  
Adjou Ané
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Anthracis ◽  
Structural Modification ◽  
Antibacterial Activities ◽  
Pyrimidine Derivative ◽  
Pyrimidine Derivatives ◽  
Design Synthesis ◽  
Secretion Pathway

Abstract SecA, a key component of bacterial Sec-dependent secretion pathway, is an attractive target for exploring novel antimicrobials. Along this line, we reported optimization of a hit bistriazole (SCA 21) which has been previously identified as a SecA inhibitor. Herein we describe the synthesis of some novel triazole-pyrimidine derivative by structural modification of SCA 21. Some of them have been evaluated for their antimicrobial activity against against Escherichia coli NR698 (a leaky mutant), Staphylococcus aureus and Bacillus anthracis Sterne.

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