scholarly journals Chemical characterisation and in vitro assessment of two mushroom stalks as prebiotics for Clarias gariepinus (Burchell, 1822)

2021 ◽  
Vol 54 (1) ◽  
pp. 104-115
Author(s):  
Oluwakemi Abimbola Adejonwo ◽  
Bamidele Oluwarotimi Omitoyin ◽  
Olugbenga Adeniran Ogunwole ◽  
Emmanuel Kolawole Ajani ◽  
Siyanbola Adewumi Omitoyin

Abstract The potentials of mushroom stalks as supplements in aqua-feeds have been grossly underutilised. Stalk meals of two Pleurotus species were analysed for proximate composition, fibre fractions, mineral and phytochemical constituents. In vitro digestibility and fermentability were assessed using caecal digesta from Clarias gariepinus (n = 108; weight: 138 ± 10.8 g). Stalks of Pleurotus pulmonarius and Pleurotus ostreatus were air-dried at ambient room temperature and milled. Pleurotus ostreatus contained higher (P < 0.05) moisture, crude protein, ether extract and crude fibre than P. pulmonarius stalks which had higher (P < 0.05) nitrogen-free extract. Pleurotus ostreatus had higher (P < 0.05) neutral detergent fibre, acid detergent fibre, acid detergent lignin and cellulose but lower (P > 0.05) hemicellulose than P. pulmonarius. Except in manganese and iron content, P. ostreatus contained higher (P < 0.05) sodium, calcium, magnesium, potassium, phosphorus, copper and zinc than P. pulmonarius. Both stalks contained flavonoids, phlobatannin, terpenoid, cardiac glycosides, steroids and antraquinone. Substrate loss was higher (P < 0.05) in P. pulmonarius (0.20 g) than P. ostreatus (0.15 g). Maximum rate of gas production was more (P < 0.05) for P. pulmonarius (0.16 mL/h) at 4.96 hours compared to 0.04 mL/h at 6.04 hours for P. ostreatus. Both stalk meals were partially resistant to in vitro digestibility and were fermentable. Hence, they possess favourable prebiotics characteristics and can be used as supplement in aqua feed.

2020 ◽  
Vol 36 (2) ◽  
pp. 313-324
Author(s):  
O. J. Babayemi ◽  
F. K. Otukoya ◽  
F. O. Familade ◽  
M. O. Daodu

Corn-straw (CS) and corn-cob (CC) were treated with bovine liquor (BL) to enhance their nutritive values. Eight preparations consisting of CC, CS, CC + BL, CS + BL, CC + urea, CS+ urea, CC+ BL+urea and CS + BL + urea were made to ferment for five days under anaerobic condition. The final products were assessed for their chemical composition, in vitro gas production and feed preference by sheep and goats. Crude protein contents in CC and CS significantly (P <0.05) increased from 3.5% and 2.7% to 8.6% and 3.4% respectively without urea but addition of urea increased the contents to 14.6% and 6.8% respectively. Crude fibre, neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose and hemicellulose of the treated CS and CC were significantly (P < 0.05) reduced. Similar trends were observed for gas production characteristics as the potentially degradable fraction ‘b’ and the potential degradability ‘a + b’ of treated straw and cobs were better (P < 0.05) than the untreated. The rate ‘c’ of gas production was slower for the treated than the untreated CC and CS. Both sheep and goats separately preferred the treated CC and CS to the untreated after three days and subsequent days of consumption. Since the bovine liquor showed the tendency of enhancing the nutrient contents of corn-cobs and corn-straw and that small ruminant preferred the treated materials than the untreated, sheep and goats can be sustained during the dry season.


2020 ◽  
Vol 50 (1) ◽  
pp. 161-169
Author(s):  
İ Ülger ◽  
S.B. Beyzi ◽  
M Kaliber ◽  
Y Konca

Quality attributes of citrus pulp silages that were ensiled alone and combined with maize silage were determined. Fresh samples of lemon, orange and tangerine pulps, maize plants and their combinations were fermented in glass jars for 90 days at about 20 - 25 °C. Treatments included i) 100% maize silage as control (MS); ii) 100% lemon pulp silage (LPS); iii) 100% orange pulp silage (OPS); iv) 100% tangerine pulp silage (TPS); v) 50% LPS and 50% maize silage (LPS + MS); vi) 50% OPS and 50% MS; and vii) 50% TPS and 50% maize silage (TPS + MS). The pH differed among treatment groups. The highest and lowest pH values were recorded for MS group and the OPS + MS group, respectively (3.84 vs. 3.51). The highest dry matter (DM), crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF) and hemicellulose (HEM) were observed for MS (P <0.01). Citrus pulp silages alone had significantly greater total digestible nutrients (TDN), organic matter (OM) and non-fibre carbohydrate (NFC) values than MS and the combined silages (P <0.01). Maize silage had higher lactic acid (LA) (101.2 g/kg DM) and acetic acid (AA) (49.3 g/kg DM) concentrations than the citrus groups (P <0.05). In vitro gas production (TG), methane (CH4), metabolizable energy (ME) and organic matter digestibility (OMD) of the silages were similar (P >0.05). Thus, citrus pulps can be ensiled in spite of their moisture content. However, to increased DM and nutrient content, the citrus pulps should be ensiled with 50% maize. Keywords: fermentation metabolites, methane production, organic matter digestibility


Author(s):  
D. T. Q. Carvalho ◽  
A. R. F. Lucena ◽  
T. V. C. Nascimento ◽  
L. M. L. Moura ◽  
P. D. R. Marcelino ◽  
...  

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.


1972 ◽  
Vol 25 (6) ◽  
pp. 1377 ◽  
Author(s):  
RFH Dekker ◽  
GN Richards

The dry matter digestibility (DMD) of poor quality roughages such as spear grass (Heteropogon contortus) by sheep and cattle has been shown to increase on addition of sulphur to the diet. The sulphur is added most frequently as inorganic sulphate, either alone (Playne 1969) or supplemented with urea (Bray and Hemsley 1969; Kennedy and Siebert 1972; Siebert and Kennedy 1972) or gluten (Playne 1969). These supplements have also increased feed intake in sheep (Playne 1969; Kennedy and Siebert 1972; Siebert and Kennedy 1972) and cattle (Siebert and Kennedy 1972), and have improved the nitrogen and sulphur balance (Moir, Somers, and Bray 1967; Bray and Hemsley 1969; Playne 1969; Kennedy and Siebert 1972) within the animal. Sulphates have been shown to increase the in vitro rumen digestion of starch (Kennedy, Mitchell, and Little 1971), crude fibre (Bray and Hemsley 1969), and acid-detergent fibre and cellulose (Barton, Bull, and Hemken 1971).


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 393-394
Author(s):  
Adebayo O Oni ◽  
Bobby-Joe Ogadu ◽  
Azeez O Yusuf ◽  
A Adebowale ◽  
Oluwakemi Oni ◽  
...  

Abstract Recently, bioactive component of plant and plant parts have been used as rumen modifier to reduce methane gas production in ruminant livestock so as to reduce their contribution to the implicated greenhouse effect. This study therefore, evaluated the potential of Siam weed (Chromolaena odorata) leaves as a modifier of rumen fermentation in West African dwarf bucks. Fresh C. odorata leaves were harvested, air dried (3 weeks), milled using a 2mm sieve size and bagged for both proximate and phytochemical analysis. Concentrate diets were formulated with C. odorata leaf meal included in the diet at 0, 2, 4 and 6% of the whole diet. Rumen fluids were collected from West African dwarf (WAD) bucks (averaged 25kg) using suction tube and randomly allotted to the 4 experimental diets in a Completely Randomized Design. Incubation of inoculums was done for 96hrs with 12 replicates per treatment in a single run. Data obtained were analyzed using a One-way Analysis of Variance and means compared using Tukey’s Test. Results indicated that C. odorata had 969.0mg/kg dry matter, 175.1mg/kg crude protein, 204.3mg/kg crude fibre, 521.6mg/kg nitrogen free extract, 19.9 mg/kg saponin, 25.7 mg/kg tannin, 10.8mg/kg flavonoid and 12.6 mg/kg alkaloid. The addition of 2 and 4% C. odorata to the diets resulted in increased (P &lt; 0.05) in vitro gas production while C. odorata at 2 % reduced (P &lt; 0.05) the methane gas (%) estimate. In vitro organic and dry matter digestibilities, total digestible substrates and short chain fatty acids were increased (P &lt; 0.05) with C. odorata addition to the diets. This study concluded that the use of C. odorata as an additive at 2 and 4% inclusion increased total gas output; however, 2% inclusion will be beneficial as it reduced the methane output while maintaining higher gas production and digestibility.


2014 ◽  
Vol 65 (5) ◽  
pp. 479 ◽  
Author(s):  
Belete Shenkute Gemeda ◽  
Abubeker Hassen

This study characterised 16 tropical perennial grass species in terms of in vitro methane output and related their digestibility and rumen fermentation with methane output. The grass samples were collected, dried in a forced oven, and ground and analysed for nutrient composition. In vitro gas production and organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure gas production through in vitro incubation at 39°C. Anthephora argentea and Stipagrostis ciliate produced the highest concentration of methane in terms of g kg–1 digestible dry matter (DDM) and g kg–1 digestible organic matter (IVOMD). Cenchrus ciliaris, Setaria verticillata and Panicum coloratum produced the lowest (P < 0.05) methane when expressed in terms of g kg–1 DDM and g kg–1 IVOMD. Ash, ether extract, non-fibrous carbohydrate, neutral and acid detergent insoluble nitrogen, and crude protein were negatively correlated with methane production. Methane production positively correlated with neutral and acid detergent fibre, cellulose and hemicellulose. It is important to focus on screening and selecting perennial grass with higher nitrogen content and low methane production to mitigate methane production under tropical conditions.


2021 ◽  
Vol 15 ◽  
pp. 161-166
Author(s):  
T. A. ADEGBOLA ◽  
R. C. OGBONNA ◽  
N. E. NWACHUKWU

Dry matter intake, nutrient digestibility and some rumen and blood metabolites were investigated in goats fed five diets composed of cassava peel and brewer's dried grains (BDG) in the ratios of approximately 82:8, 69:20, 57:33, 44:45 and 32:58. Urea and molasses were included in each diet at 2% and 7.5% respectively and the crude protein levels of the diets were approximately 8%, 11%, 14%, 17% and 20%. There were no significant (P > 0.05) differences in Dry matter and Organic matter intake but N intake Increased up to Diet 4. There were dietary effects on the digestibility of DM, N, crude fibre, acid detergent fibre, either extract, nitrogen free extract and energy but not 0M. The highest digestibility values were obtained on diet 4 for most of the nutrients. The concentrations of rumen ammonia and blood plasma urea increased from Diet 1 to Diet 5. There were significant (P < 0.05) dietary effect on rumen fluid ph. Rumen protozoal count increased from Diet 1 to Diet 5. The depression in digestibility of Diet 5 would indicate that BDG should not be included at up to 58% in the diet of goats.


2020 ◽  
Vol 45 (4) ◽  
pp. 203-209
Author(s):  
U. Okpanachi ◽  
C. I. Agu ◽  
L. E. Igoche ◽  
F. A. Oyedapo

The study was aimed at assessing the effects of two varieties of cashew (Anacardium occidentale) apple pulp as feedstuff for ruminants. Samples of the yellow and red varieties of cashew apple pulp were collected, processed and analysed for mineral, crude fiber fraction and pH. Results showed that sodium value in red variety was significantly higher (P<0.05) than that of the yellow variety. Potassium value in red variety was significantly higher (P<0.01) than that of the yellow variety. Values for calcium, magnesium, and phosphorus in the yellow variety were significantly higher (P<0.01) than those of the red variety. Values for cellulose, hemicelluloses and neutral detergent fibre in the red variety were significantly higher (P<0.01) than those of the yellow variety. Acid Detergent Lignin value for the yellow variety was significantly higher (P<0.01) than that of the red variety. Values for acid detergent fibre, crude protein, carbohydrate and pH were not significantly affected (P>0.05). Yellow cashew apple pulp from the results of this study is richer in minerals when compared to the red variety. Results from the crude fibre fraction suggest that the yellow cashew apple pulp variety may have a better digestibility when compared to the red variety. Although the values of the various parameters from the 2 varieties show that they are fit to be used as feeding stuff for ruminants, the yellow variety appears to be better on the general basis.


2020 ◽  
Vol 45 (1) ◽  
pp. 309-316
Author(s):  
O. O. Olufayo ◽  
O. O. Falola

The effect of processing on proximate, mineral, anti-nutritional compositions, gasproduction characteristics, methane production, predicted metabolizable energy, organic matter digestibility and short chain fatty acids production of Delonix regia seeds incubated with Pennisetum purpureum were determined. Five treatments (T1: mixture of soaked Delonix regia seed and Pennisetum purpureum; T2: mixture of raw Delonix regia seed and Pennisetum purpureum; T3: mixture of roasted Delonix regia seed and Pennisetum purpureum; T4: mixture of boiled Delonix regia seed and Pennisetum purpureum and T5:100% Pennisetum purpureum which serves as control) were designed. In vitro gas production techniques for 24 hours were used to determine the nutritive value of processed 50% Delonix regia seed and 50% Pennisetum purpureum. The crude protein ranged from 13.38 to 15.71g/100g DM among the treatments while crude fibre was between 20.10 and 21.40g/100g DM, ether extract ranged from 1.90 to 3.12g/100g DM and ash 7.77 to 12.34g/100g DM. Calcium ranged from (2.34 - 3.61%), magnesium ranged (2.90 – 3.48%), sodium (2.05 – 2.91%) and phosphorus (0.88 – 1.12%). It was observed generally that T2 recorded the highest among the treatments while the values obtained for the heat treated seeds were lower than the raw. Oxalates ranged between (0.23% - 0.32%), phytates (0.29 – 0.43%), tannin (0.03 – 0.08%) and saponnin (0.36 – 0.49%). Methane (ml/200mg DM) production indicated T3 (50% roasted Delonix regia seed and 50% Pennisetum purpureum) was highest. The potential gas production 'a+b' ranged from 12.33 to 28.33mL/200mg DM). The highest potential gas production 'a+b' value of 28.33mL/200mg DM was obtained in T1 compared to other dietary treatment. Dry matter digestibility (DMD) ranged between 70.23 – 91.02% while the rate of fermentation was between 0.51 and 1.18ml/hr. The rate of fermentation was directly proportional to dry matter digestibility (DMD); the soaked recorded the highest value. Processing enhanced the nutritional contents of Delonix regia seeds. The result obtained showed that there were significant differences (p<0.05) among the treatments. In conclusion, soaking the seeds of Delonix regia had beter nutritional contents and has the potential for dry season feeding.  


2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.


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