Effects of GA3 and ABA on the Germination of Dormant Oat Seeds

Abstract Oat seed with dormancy characteristics, which can germinate after one season or one year, are used to build and maintain vegetation to protect soils from been damaged by desertification in Northern China. The aim of this study was to estimate the effects of endogenous and exogenous GA3 and ABA on oat seed (var. Baiyan 7) germination. The results showed that seeds without peel hull had lower endogenous ABA content and the ratio of ABA/GA3 than seeds with peel hull. The best GA3 treatment duration for milky ripe, wax ripe, full ripe seeds were 60 min or 120 min, 60 min and 30 min, respectively. Seed germination rate, germination potential and germination index increased before they declined with the increasing of GA3 concentrations. The best GA3 concentration treatment was 100 mg/l, while the turning point was 200 mg/l. The dormancy rate of low temperature storage seeds were higher than those of room temperature storage seeds at each storage time, and both decreased with the increase of the storage time. For the seeds which were new or stored for 1-2 months, the germination rates were enhanced significantly by exogenous GA3. For the seeds that had been stored for over three months, GA3 treatment had no effect on germination rate. Germination rate decreased with the increase of ABA concentrations. The most inhibitive effect, which leaded to a seed germination reduction by 37.7% and 4.0%, appeared, when the concentration of ABA was 500 mg/L and 1000 mg/l, respectively. GA3 could abate the effect which ABA inhibited seed germination.

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Storage of strawberry pollen

Acta Agrobotanica ◽

10.5586/aa.2001.028 ◽

2013 ◽

Vol 54 (2) ◽

pp. 117-121

Author(s):

Rafet Aslantaş ◽

Lütfi Pirlak

Keyword(s):

Pollen Germination ◽

Room Temperature ◽

Sucrose Solution ◽

Germination Rate ◽

Storage Period ◽

Low Temperature Storage ◽

Different Temperatures ◽

One Year ◽

Hanging Drop Method ◽

Temperature Storage

This study was carried out to determine storage ability of strawberry pollen at different temperatures for three different strawberry cultivars 'Aliso', 'Brio', and 'Cruz'. Strawberry pollen was stored at room temperature (22 ±2°C), +4°C, -4°C and -18°C in stabile humidity conditions. Strawberry poIlen was germinated using the hanging drop method in a 20% sucrose solution. Pollen germination rate increased because of low temperature storage. Pollen stored at room temperature and +4°C, -4°C, and -18°C was kept for 8 months, about one year, and 20 months, respectively. Pollen germination rates decreased as the length of storage period increased. The reaction of all cultivars tested on the duration and temperature of storage was similar.

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Study on Changes of Textural and Biochemical Properties of Tuna during Ultra-Low Temperature Storage

Environment and Natural Resources Research ◽

10.5539/enrr.v6n2p51 ◽

2016 ◽

Vol 6 (2) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Yu-ying PAN ◽

Xiao-hua QIU ◽

Jin-sheng YANG

Keyword(s):

Soluble Protein ◽

Storage Time ◽

Storage Temperature ◽

Frozen Storage ◽

Biochemical Properties ◽

Texture Profile ◽

Low Temperature Storage ◽

Different Temperatures ◽

Temperature Storage

The effect of TPA and biochemical properties of Yellow Tuna during frozen storage at different temperatures(-18°C, -25°C, -35°C, -45°C, -55°C, -65°C) were studied by measuring the textural characteristics (the hardness, Springiness) salt-solubility of myofibrillar proteins, Ca2+ATPase activities. The results indicated that the hardness, springiness, actomyosin salt-solubility, Ca2+ATPase activities decreased during the process of frozen storage. Meanwhile, the frozen stored temperature showed great effect on the freezing denaturation of protein (P < 0.05). For the same longer of the storage time, the lower frozen temperature, the less extent of freeze denaturation; Stored in -18°C for three months, the content of Salt soluble protein reduced to zero; Stored in -25°C for 120 days, the content of salt soluble protein also reduced to zero; But stored in -55°C and -65°C, the change is very little. Ca2+ATPase activities also reduced to zero after stored in -18°C and -25°C for three months. But stored in -55°C and -65°C, there is no obvious change. Moreover, there is a Positive relationship between the change of texture profile and the content of Salt soluble protein, the lower the storage temperature, the less of the change of texture profile. Therefore, when it is stored in -55°C, the quality of Yellow Tuna can be maintained to the maximum extent within six months.

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GERMINATION OF STORED SEEDS OF FOUR TREE SPECIES FROM THE TROPICAL DRY FOREST OF MORELOS, MEXICO

Botanical Sciences ◽

10.17129/botsci.96 ◽

2014 ◽

Vol 92 (2) ◽

pp. 281 ◽

Cited By ~ 3

Author(s):

Martha Cervantes ◽

Eliane Ceccon ◽

Consuelo Bonfil

Keyword(s):

Seed Germination ◽

Seed Size ◽

Storage Time ◽

Germination Rate ◽

Tropical Dry Forest ◽

Dry Forest ◽

Tropical Dry Forests ◽

Germination Capacity ◽

Dry Forests ◽

And Storage

Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in Acacia bilimekii, Haematoxylum brasiletto, Lysiloma acapulcense, and L. divaricatum. Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two Lysiloma species, but in A. bilimekii there were large differences among provenances. Mean germination capacity was low in H. brasiletto after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests.

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Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211013 ◽

2020 ◽

Vol 21 (10) ◽

Author(s):

ISKANDAR Z. SIREGAR ◽

KARIMA FAUZIAH MUHARAM ◽

Y. ARIS PURWANTO ◽

DEDE J. SUDRAJAT

Keyword(s):

Seed Germination ◽

Storage Time ◽

Physiological Activity ◽

Germination Rate ◽

Seed Viability ◽

Seed Priming ◽

Germination Capacity ◽

Gmelina Arborea ◽

Randomized Design ◽

Germination Characteristics

Abstract. Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564. Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year. On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.

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Effects of temperature, light and other treatments on seed germination of Leymus chinensis

Canadian Journal of Plant Science ◽

10.4141/p04-125 ◽

2006 ◽

Vol 86 (1) ◽

pp. 143-148 ◽

Cited By ~ 13

Author(s):

W. D. Zhang ◽

J. J. Bi ◽

T. Y. Ning ◽

X. L. Liu ◽

M. R. He

Keyword(s):

Seed Germination ◽

Sand Dune ◽

Germination Rate ◽

Seed Priming ◽

Northern China ◽

Leymus Chinensis ◽

Cool Season ◽

Effects Of Temperature ◽

Cool Season Grass ◽

Improve Seed Germination

Leymus chinensis (Trin.) Tzvel., a perennial cool-season grass distributed widely in eastern Asia, is an ideal species for the rangeland industry and soil conservation, but the seed has a long dormant period and low germination rates. This study examined the effects of temperature, light and various treatments including stratification, H2SO4, NaOCl, gibberellic acid (GA3) and 6-benzyl adenine (BA), on the germination of Leymus chinensis seed. Seed stored over 1 yr and germinated at a temperature regime of 25/15°C with a 12/12-h photoperiod germinated best, and had a germination rate of 30.3%, with a minimum germination time of 12 d. In these germination conditions, germinated seeds were counted after 15 d to determine the effects of other treatments upon freshly harvested seed germination. The final germination rate for freshly harvested seeds stratified at 4°C for 0 to 10 wk ranged from 8.5 to 37.3%. Germination rates were 14.0 and 14.8% when seeds were treated with 70% H2SO4 for 3 and 6 min, respectively. Seed treated with 5.0% NaOCl germinated 17.5 and 16.0% when treated for 10 and 20 min, respectively. When seeds were soaked in 1000 mg L-1 GA3 solution for 24 h, 42.3% germinated, while treatment with 50 mg L-1 of BA resulted in 50.3% germination. The results indicate that various dormancy mechanisms may exist in the species. These findings will be helpful to farmers and breeders in selecting suitable treatments to improve seed germination and establishment. Key words: Seed dormancy, grassland and sand-dune reclamation, photoperiod, Northern China, Poaceae, seed priming

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STORAGE OF ISOLATED CHLOROPLASTS WITHOUT LOSS OF PHOTOCHEMICAL ACTIVITY

Canadian Journal of Research ◽

10.1139/cjr50c-030 ◽

1950 ◽

Vol 28c (5) ◽

pp. 513-524 ◽

Cited By ~ 20

Author(s):

P. R. Gorham ◽

K. A. Clendenning

Keyword(s):

Low Temperature ◽

Plant Species ◽

Storage Temperature ◽

Photochemical Activity ◽

Low Temperature Storage ◽

Spinach Chloroplasts ◽

One Year ◽

The Stability ◽

Isolated Chloroplasts ◽

Temperature Storage

Fragmented spinach chloroplasts were stored for one year in 0.5 M sucrose at −40 °C. with almost negligible losses of photochemical activity. Thawed chloroplasts and freshly isolated chloroplasts showed similar rates of deterioration at 10 °C. Washed chloroplasts did not show as high retention of activity during low temperature storage as crude chloroplast suspensions. High retentions of activity were observed with chloroplasts from different plant species, as well as with different oxidants. The activities of subsamples that were frozen and thawed in a standard way agreed within 5%. Deterioration during lyophilization of chloroplasts occurred chiefly during the early stages of drying, and was lessened by sucrose. The stability of lyophilized chloroplasts in storage varied directly with the degree of desiccation and inversely with the storage temperature; little deterioration occurred in thoroughly dried chloroplasts during lengthy storage at or below 5 °C.

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LOW-TEMPERATURE STORAGE OF BEDDING PLANT PLUGS

HortScience ◽

10.21273/hortsci.27.6.603b ◽

1992 ◽

Vol 27 (6) ◽

pp. 603b-603

Author(s):

Royal D. Heins ◽

Nathan Lange ◽

Thomas F. Wallace

Keyword(s):

Storage Time ◽

Chilling Injury ◽

Photon Flux ◽

Low Temperature Storage ◽

Bedding Plant ◽

Effects Of Temperature ◽

Delayed Flowering ◽

And Storage ◽

Storage Temperatures ◽

Temperature Storage

Ageratum, begonia, marigold, and salvia seedlings in plug cells were stored in coolers to determine the effects of temperature, light, and storage time on growth and forcing time of seedlings after transplanting, and to determine the optimum storage temperatures for each crop. Photosynthetic photon flux densities of 0, 1, and 5 μmol·m-2.s-1 were combined with temperatures of 0.0, 2.5, 5.0, 7.5, 10.0, and 12.5C to create 18 storage environments. Sample plants were removed from each treatment at 1-week intervals for 6 weeks, and were forced into flower. In all four species, temperatures of 0.0 and 2.5C caused chilling injury and then death as plants were stored for progressively longer periods. Storage at 0.0 and 2.5C also delayed flowering when chilling injury was not severe enough to cause death. In general, plants stored better in the light than in darkness. Darkness tended to limit the time seedlings could be stored, but for each crop, the addition of just 1 μmol·m-2.s-1 extended the storage durations to 6 weeks at one or more temperatures. Storage of all four species was possible for 6 weeks, but there were significant variations between the temperatures and storage durations each species could tolerate. Optimal temperatures were 5-7.5C for begonia, 5C for marigold, and 7.5C for salvia and ageratum.

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CHANGE OF SEED GERMINATION RATE WITH STORAGE TIME OF Santalum album l. (INDIAN SANDALWOOD) SEEDS

Proceedings of International Forestry and Environment Symposium ◽

10.31357/fesympo.v15i0.209 ◽

2012 ◽

Vol 15 (0) ◽

Author(s):

Y.MY.M.M. Gamage ◽

S.M.C.U.P. Subasinghe ◽

D.S. Hettiarachchi

Keyword(s):

Seed Germination ◽

Storage Time ◽

Germination Rate ◽

Santalum Album ◽

Santalum Album L

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Exogenous Melatonin accelerates seed germination in cotton(Gossypium hirsutum L.)

10.1101/618959 ◽

2019 ◽

Author(s):

Shuang Xiao ◽

Liantao Liu ◽

Hao Wang ◽

Dongxiao Li ◽

Zhiying Bai ◽

...

Keyword(s):

Seed Germination ◽

Plant Hormones ◽

Cotton Seed ◽

Germination Rate ◽

Melatonin Treatment ◽

Low Concentrations ◽

Vigor Index ◽

Cotton Seeds ◽

High Concentrations ◽

Aba Content

AbstractSeed germination is considered as the beginning of the spermatophyte lifecycle, as it is a crucial stage in determining subsequent plant growth and development. Although many previous studies have found that melatonin can promote seed germination, the role of melatonin in cotton germination remains unexamined. The main objective of this study is the characterization of potential promotional effects of melatonin (at doses of 0, 10, 20, 50, 100 and 200 μM) on cotton seed germination. This experiment demonstrated that low concentrations of melatonin can promote germination, while high concentrations failed to promote germination and even inhibited germination. Together, these results indicate that a 20 μM melatonin treatment optimally promotes cotton seed germination. Compared with the control, germination potential (GP), germination rate (GR) and final fresh weight (FW) increased by 16.67%, 12.30% and 4.81% respectively. Although low concentrations of melatonin showed some improvement in vigor index (VI), germination index (GI) and mean germination time (MGT), these effects did not reach significant levels. Antioxidant enzyme activity during seed germination was most prominent under the 20 μM melatonin treatment. Superoxide dismutase (SOD) and peroxidase (POD) activities were significantly increased by 10.37–59.73% and 17.79–47.68% compared to the melatonin-free control. Malondialdehyde (MDA) content was reduced by 16.73–40.33%. Two important plant hormones in seed germination were also studied. As melatonin concentration increased, ABA content in seeds decreased first and then increased, and GA3 content showed a diametrically opposite trend, in which the 20 μM melatonin treatment was optimal. The 20 μM melatonin treatment reduced ABA content in seeds by 42.13–51.68%, while the 20 μM melatonin treatment increased GA3 content in seeds to about 1.7–2.5 times that of seeds germinated without melatonin. This study provides new evidence suggesting that low concentrations of melatonin can promote cotton seed germination by increasing the activity of antioxidant enzymes, thereby reducing the accumulation of MDA and regulating plant hormones. This has clear applications for improving the germination rate of cotton seeds using melatonin.

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Melatonin improves the germination rate of cotton seeds under drought stress by opening pores in the seed coat

PeerJ ◽

10.7717/peerj.9450 ◽

2020 ◽

Vol 8 ◽

pp. e9450

Author(s):

Yandan Bai ◽

Shuang Xiao ◽

Zichen Zhang ◽

Yongjiang Zhang ◽

Hongchun Sun ◽

...

Keyword(s):

Drought Stress ◽

Seed Germination ◽

Germination Rate ◽

Soluble Sugars ◽

Physiological Mechanism ◽

Absorption Capacity ◽

Positive Role ◽

Cotton Seeds ◽

Aba Content ◽

Germination And Growth

The germination of cotton (Gossypium hirsutum L.) seeds is affected by drought stress; however, little is known about the physiological mechanism affecting germination and the effect of melatonin (MT) on cotton seed germination under drought stress. Therefore, we studied the effects of exogenous MT on the antioxidant capacity and epidermal microstructure of cotton under drought stress. The results demonstrated a retarded water absorption capacity of testa under drought stress, significantly inhibiting germination and growth in cotton seeds. Drought stress led to the accumulation of reactive oxygen species (ROS), malondialdehyde (MDA), and osmoregulatory substances (e.g., proline, soluble protein, and soluble sugars); it also decreased the activity of antioxidant enzymes and α-amylase. Drought stress inhibited gibberellin acid (GA3) synthesis and increased abscisic acid (ABA) content, seriously affecting seed germination. However, seeds pre-soaked with MT (100 µM) showed a positive regulation in the number and opening of stomata in cotton testa. The exogenous application of MT increased the germination rate, germination potential, radical length, and fresh weight, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), and α-amylase. In addition, MT application increased the contents of organic osmotic substances by decreasing the hydrogen peroxide (H2O2), superoxide anion (O2-), and MDA levels under drought stress. Further analysis demonstrated that seeds pre-soaked with MT alleviated drought stress by affecting the ABA and GA3 contents. Our findings show that MT plays a positive role in protecting cotton seeds from drought stress.

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