Effects of temperature, light and other treatments on seed germination of Leymus chinensis

2006 ◽  
Vol 86 (1) ◽  
pp. 143-148 ◽  
Author(s):  
W. D. Zhang ◽  
J. J. Bi ◽  
T. Y. Ning ◽  
X. L. Liu ◽  
M. R. He
Keyword(s):  
Seed Germination ◽  
Sand Dune ◽  
Germination Rate ◽  
Seed Priming ◽  
Northern China ◽  
Leymus Chinensis ◽  
Cool Season ◽  
Effects Of Temperature ◽  
Cool Season Grass ◽  
Improve Seed Germination

Leymus chinensis (Trin.) Tzvel., a perennial cool-season grass distributed widely in eastern Asia, is an ideal species for the rangeland industry and soil conservation, but the seed has a long dormant period and low germination rates. This study examined the effects of temperature, light and various treatments including stratification, H2SO4, NaOCl, gibberellic acid (GA3) and 6-benzyl adenine (BA), on the germination of Leymus chinensis seed. Seed stored over 1 yr and germinated at a temperature regime of 25/15°C with a 12/12-h photoperiod germinated best, and had a germination rate of 30.3%, with a minimum germination time of 12 d. In these germination conditions, germinated seeds were counted after 15 d to determine the effects of other treatments upon freshly harvested seed germination. The final germination rate for freshly harvested seeds stratified at 4°C for 0 to 10 wk ranged from 8.5 to 37.3%. Germination rates were 14.0 and 14.8% when seeds were treated with 70% H2SO4 for 3 and 6 min, respectively. Seed treated with 5.0% NaOCl germinated 17.5 and 16.0% when treated for 10 and 20 min, respectively. When seeds were soaked in 1000 mg L-1 GA3 solution for 24 h, 42.3% germinated, while treatment with 50 mg L-1 of BA resulted in 50.3% germination. The results indicate that various dormancy mechanisms may exist in the species. These findings will be helpful to farmers and breeders in selecting suitable treatments to improve seed germination and establishment. Key words: Seed dormancy, grassland and sand-dune reclamation, photoperiod, Northern China, Poaceae, seed priming

scholarly journals Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh
Keyword(s):  
Seed Germination ◽  
Climate Variability ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Seedling Development ◽  
Hot Water ◽  
Crop Failure ◽  
Simple Method ◽  
Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

scholarly journals Muskmelon seed germination and seedling development in response to seed priming

2003 ◽  
Vol 60 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Germination Rate ◽  
Fungal Growth ◽  
Seed Priming ◽  
Seedling Development ◽  
Factors Affecting ◽  
Total Seed ◽  
Cucumis Melo L ◽  
Osmotic Potentials

Important factors affecting seed priming have not been extensively reported in muskmelon (Cucumis melo L.) studies. The optimization of the seed priming technique becomes very important at the commercial scale. Little information has been reported on seedling development of muskmelon subsequent to seed priming. Seeds of muskmelon were primed in darkness at 25°C in different solutions and three osmotic potentials. Seeds were also primed with and without aeration during different periods. In relation to osmotic solutions, an osmotic potential around -1.30 MPa is most adequate for muskmelon priming. Salt solutions gave better germination rate but were deleterious for seed germination, especially at higher osmotic potentials. Aeration of the soaking salt solution gave faster germination at 17°C, and because of the early germination, these treatments probably presented a better seedling development. Deleterious effect on total seed germination was observed for long soaking periods with aeration. Fungal growth increased on seeds primed in aerated solutions. Seeds from priming treatments had a better germination rate and seedling development under 17 and 25°C.

scholarly journals The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Principal Component ◽  
Dry Weight ◽  
Seed Priming ◽  
Nacl Stress ◽  
Future Research ◽  
Fresh Weight ◽  
Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea maysL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Tian ◽  
Bo Guan ◽  
Daowei Zhou ◽  
Junbao Yu ◽  
Guangdi Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Yield ◽  
Seedling Growth ◽  
Germination Rate ◽  
Seed Priming ◽  
Grain Weight ◽  
Shoot Biomass ◽  
Control Group ◽  
Yield Traits ◽  
Seed Pretreatment

A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea maysL.). Results indicated that the seeds primed by gibberellins (GA), NaCl, and polyethylene glycol (PEG) reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P<0.05). The recommended prime reagents were GA at 10 mg/L, NaCl at 50 mM, and PEG at 15% on account of germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM), or PEG (15%) significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

scholarly journals Seed Enhancements to Improve Spinach Germination

HortScience ◽  
2001 ◽  
Vol 36 (5) ◽  
pp. 979-981 ◽  
Author(s):  
Leslie S. Katzman ◽  
Alan G. Taylor ◽  
Robert W. Langhans
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Spinacia Oleracea ◽  
Germination Rate ◽  
Component Analysis ◽  
Seed Treatments ◽  
Germination Time ◽  
High Uniformity ◽  
Germination Temperature ◽  
Improve Seed Germination

Rapid, synchronized, and high percentage of germination is required for commercial spinach (Spinacia oleracea L.) production using hydroponic techniques. Seed treatments examined to improve seed germination were: 1) decoating; 2) leaching in water; and 3) soaking seeds for 4 hours in 0.5% NaOCl, leaching for 15 hours in water, and sowing in 0.3% H2O2 (this treatment will be referred to as NaOCl/H2O2). Germination studies were conducted on four cultivars at a constant 18 °C (optimal) or 30 °C (inhibitory). At 18 °C, germination rate (T50) was maximized by both hydration treatments, but uniformity of germination (Tsd) was greatest for decoated seeds; final germination was ≥89% for all treatments. At 30 °C, decoating resulted in greatest uniformity of germination. The NaOCl/H2O2 treatment resulted in highest germination (94%) at the high temperature, whereas decoating was least effective (69%). Reduced germination of decoated seeds was attributed to atypical germinants. Cultivars differed in response to the treatments at both temperatures. Component analysis of the NaOCl/H2O2 treatment was studied with two slow-to-germinate cultivars. Treatment with H2O2, with or without NaOCl, improved the rate, uniformity, and percentage of germination of seeds of both cultivars, but NaOCl alone did not. Pericarp removal or pericarp removal plus NaOCl/H2O2 treatments reduced variability in germination time and enhanced speed of germination at 30 °C, but decoating produced a higher percentage of atypical seedlings than did other treatments. Therefore, the NaOCl/H2O2 treatment is recommended for growers who are unable to maintain cool germination temperatures and/or cannot afford the costs associated with cooling. If growers can maintain a germination temperature of ≈18 °C, decoated seeds are preferable, based on the high uniformity of germination.

Effects of GA3 and ABA on the Germination of Dormant Oat Seeds

2018 ◽  
Vol 51 (3) ◽  
pp. 25-41 ◽  
Author(s):  
J. Ge ◽  
Y Hu ◽  
C Ren ◽  
L Guo ◽  
C Wang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Turning Point ◽  
Germination Rate ◽  
Northern China ◽  
Low Temperature Storage ◽  
Oat Seeds ◽  
One Year ◽  
Aba Content ◽  
Temperature Storage

Abstract Oat seed with dormancy characteristics, which can germinate after one season or one year, are used to build and maintain vegetation to protect soils from been damaged by desertification in Northern China. The aim of this study was to estimate the effects of endogenous and exogenous GA3 and ABA on oat seed (var. Baiyan 7) germination. The results showed that seeds without peel hull had lower endogenous ABA content and the ratio of ABA/GA3 than seeds with peel hull. The best GA3 treatment duration for milky ripe, wax ripe, full ripe seeds were 60 min or 120 min, 60 min and 30 min, respectively. Seed germination rate, germination potential and germination index increased before they declined with the increasing of GA3 concentrations. The best GA3 concentration treatment was 100 mg/l, while the turning point was 200 mg/l. The dormancy rate of low temperature storage seeds were higher than those of room temperature storage seeds at each storage time, and both decreased with the increase of the storage time. For the seeds which were new or stored for 1-2 months, the germination rates were enhanced significantly by exogenous GA3. For the seeds that had been stored for over three months, GA3 treatment had no effect on germination rate. Germination rate decreased with the increase of ABA concentrations. The most inhibitive effect, which leaded to a seed germination reduction by 37.7% and 4.0%, appeared, when the concentration of ABA was 500 mg/L and 1000 mg/l, respectively. GA3 could abate the effect which ABA inhibited seed germination.

scholarly journals Seed Priming with Melatonin Improves the Seed Germination of Waxy Maize under Chilling Stress via Promoting the Antioxidant System and Starch Metabolism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Qingjun Cao ◽  
Gang Li ◽  
Zhengguo Cui ◽  
Fentuan Yang ◽  
Xiaoli Jiang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Antioxidant System ◽  
Germination Rate ◽  
Chilling Stress ◽  
Seed Priming ◽  
Seed Vigor ◽  
Antioxidant Enzyme Activities ◽  
Starch Metabolism ◽  
Waxy Maize ◽  
Significant Difference

Abstract Chilling stress is one of the major abiotic stresses affecting waxy maize plant growth. Melatonin (MT) is able to improve tolerance to abiotic stress in plants. To investigate the effects of seed priming with MT on tolerance to chilling stress in waxy maize, the seed germination characteristics and physiological parameters were tested with varied MT concentrations (0, 50, 100 µM) and treatment times (12, 24 h) at ambient (25 °C) and chilling (13 °C) temperature. MT primed seeds significantly enhanced the germination potential (by 20.29% and 50.71%, respectively), germination rate (by 20.88% and 33.72%), and increased the radicle length (by 90.73% and 217.14%), hypocotyl length (by 60.28% and 136.14%), root length (by 74.59% and 108.70%), and seed vigor index (46.13%, 63.81%), compared with the non-priming seeds under chilling stress. No significant difference was found in priming time between primed and non-primed seeds. In addition, lower H2O2 and malondialdehyde concentrations, increased antioxidant enzyme activities (superoxide dismutase, peroxidase, catalase and ascorbateperoxidase), and promoted starch metabolism were found in primed seeds compared to non-primed ones. It was suggested that seed priming with MT improved waxy maize seed germination under chilling stress through improving antioxidant system and starch metabolism, which protected from oxidative damage.

scholarly journals Effect of Various Seed Priming Methods on Germination Characteristics of Black Gram

2020 ◽  
pp. 1-5
Author(s):  
Shrestha A ◽  
Keyword(s):  
Seed Germination ◽  
Seed Priming ◽  
Grain Legumes ◽  
Black Gram ◽  
Germination Characteristics ◽  
Improve Seed Germination

Black gram despite being one of the major grain legumes in the mid-hills of Nepal, poor germination is a problem in its cultivation. Seed priming is an accepted technique to improve seed germination. An experiment was therefore, conducted to study effect of priming using various priming agents on germination characteristics of Black gram.

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