scholarly journals A preliminary study on semen collection, its evaluation, and testicular and sperm morphometries in the wild proboscis monkey (Nasalis larvatus)

2021 ◽  
Vol 65 (3) ◽  
pp. 375-381
Author(s):  
Zainal Zahari Zainuddin ◽  
Mohamed Reza Mohamed Tarmizi ◽  
Yap Keng Chee ◽  
Alvin Erut ◽  
Wan Nor Fitri ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Tail Length ◽  
Proboscis Monkey ◽  
Nasalis Larvatus ◽  
Semen Collection ◽  
Semen Volume ◽  
The Social ◽  
Spherical Head ◽  
In The Wild ◽  
Digital Manipulation

Abstract Introduction The proboscis monkey (Nasalis larvatus) is an endangered species with a declining population. This article describes the first successful attempt at sperm collection and evaluation, and the testicular and sperm morphometries of the wild proboscis monkey in Sabah, Malaysia. Material and Methods Eight semen collection procedures using electro-ejaculation and digital manipulation were conducted in three wild adult male proboscis monkeys. A total of 21 ejaculates were collected. The testicular biometry was measured with the aid of ultrasonography. Sample evaluation included semen volume and pH and sperm concentration, viability, and abnormality. The sperm morphometry was undertaken using phase contrast microscopy. Results The mean (±SD) total testicular volume of these animals was 5.77 cm3 (±1.58). Semen collection by electro-ejaculation resulted in an 84% success rate, while digital manipulation did not result in any ejaculation. Each animal showed different semen characteristics, where the volume was 5–540 μL, pH 8–9, and sperm concentration 0.041–83.00 ×106/mL. The percentage of abnormal sperm was high at 76.8% (±89.60), largely due to midpiece abnormality. Normal sperm had a spherical head and long tail with a head : midpiece : tail length ratio of 1 : 2: 8. Conclusion The social status of these animals may contribute to the generally low quality of the semen. The techniques and data from this study are useful for future conservation and application of assisted reproductive technology in this species.

scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

scholarly journals Evaluation of Native Rooster Based on Breeding Value of trait of Macroscopic and Microscopic Semen Quality

2021 ◽  
Vol 16 (3) ◽  
pp. 228-232
Author(s):  
Sutiyono Sutiyono ◽  
E. Kurnianto ◽  
Sutopo Sutopo ◽  
D. K. Ariyanto ◽  
D. Samsudewa
Keyword(s):  
Data Analysis ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Breeding Value ◽  
Semen Collection ◽  
Native Chicken ◽  
Semen Volume ◽  
Individual Cage ◽  
Yellowish White

The aim of this research is to a determined the breeding value of native roosters based on macroscopic and microscopic semen. Ten native roosters were used in this research. Complete individual cage, semen collection tools and substance, macroscopic and microscopic tools, and substance were used in this research. The native rooster was rested for 2 weeks. Massage techniques were used for semen collection. Parameters observed were volume, pH, color, viscosity, smell, sperm concentration, and sperm livability. The semen collection was repeated 5 times. One-way analysis of variances was used for data analysis with the tools SPSS 16. The breeding value was estimated based on primary traits of semen and calculated for each rooster to make a rank. The results showed that significant parameters (P<0,05) are semen volume and sperm concentration, while the non-significant parameters (P?0,05) are pH semen and sperm livability. Semen color is yellowish-white, semen viscosity is medium-viscous and smell is spermin. The results of breeding value rank are rooster numbers 6, 7, 5, 3, 2, 4, 8, 9, 10, and 1. The conclusion is the breeding value of Native roosters is varied. The recommendation is Native chicken breeding need to apply selection used breeding value based on semen quality.

Effect of genetic and environmental factors on semen productionand quality of Madura cattle in Indonesia

2016 ◽  
Author(s):  
Koko Wisnu Prihatin ◽  
Luqman Hakim ◽  
Sucik Maylinda ◽  
V.M. Ani Nurgiartiningsih
Keyword(s):  
Environmental Factors ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Climatic Conditions ◽  
Bull Semen ◽  
Semen Collection ◽  
Semen Volume ◽  
Ejaculation Frequency ◽  
Genetic And Environmental Factors

The indigenous Madura cattle semen production and quality were evaluated. Total of 2275 semen collection records from 11 bulls were analyzed to evaluate the genetic potency of semen productions. The genetic evaluation was described as an estimation of bull semen volume, sperm concentration and sperm individual motility. Data were analyzed by HGLM-REML with environmental factors of age, seasons, interval of semen collections and frequency of ejaculations as fixed effect. The result showed that the age and the ejaculation frequency affected to all variables; an interval of semen collections affected to both semen volume and sperm concentrations; and seasons only affected to sperm concentrations. Repeatability estimate of semen volume, sperm concentration and sperm individual motility were 0.376, 0.445 and 0.567 respectively. It can be concluded that the quality of ejaculate was less affected by environmental factors, supported by the good adaptation of indigenous Madura cattle under the local climatic conditions and management in the artificial insemination station.

scholarly journals Establishment of an anesthetic protocol for semen collection by electroejaculation in six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758)

2016 ◽  
Vol 68 (6) ◽  
pp. 1595-1601 ◽  
Author(s):  
P.C. Sousa ◽  
R.N.L. Amorim ◽  
G.L. Lima ◽  
A.L.C. Paiva ◽  
V.V. Paula ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Membrane Integrity ◽  
Control Group ◽  
Rapid Induction ◽  
Semen Collection ◽  
Semen Volume ◽  
Functional Membrane ◽  
Euphractus Sexcinctus

ABSTRACT The aim was to verify the effects of different anesthetic protocols used during electroejaculation (EEJ) in six-banded armadillos (Euphractus sexcinctus). Four sexually matured animals were physically restrained and subjected to semen collection by the EEJ following three treatments: The control group consisted of no use of anesthesia; in the others, the anesthetic combinations xylazine/ketamine/propofol or butorphanol/ ketamine/propofol were administered. For each group, twelve procedures were conducted for EEJ. Semen was evaluated for volume, color, aspect, motility, sperm concentration, morphology, viability, and functional membrane integrity. The highest efficiency (100% ejaculates) was achieved when the control group was used; the xylazine/ketamine/propofol association provided only 11 ejaculates from a total of 12 attempts (91.6% efficiency), while only 4 ejaculates (33% efficiency) were obtained with butorphanol/ketamine/propofol (P<0.05). Both protocols provided rapid induction and relaxation enough to perform the EEJ. In the use of butorphanol/ketamine/propofol, the animals recovered at 16.5±1.5min, a time shorter than in the use of xylazine/ketamine/propofol protocol, 20.7±1.0min (P>0.05). The semen volume and sperm concentration obtained in the use of xylazine/ketamine/propofol association were significantly higher than those verified for butorphanol/ketamine/propofol protocol. In conclusion, the xylazine/ketamine/propofol association is indicated for anesthesia of six-banded armadillos submitted to EEJ.

scholarly journals Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

2021 ◽  
pp. 3156-3163
Author(s):  
Bongot Huaso Mulia ◽  
Ardyta Widianti ◽  
Jansen Manansang ◽  
Dedi Rahmat Setiadi ◽  
Vincentia Trisna Yoelinda ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Total Concentration ◽  
Sperm Concentration ◽  
Panthera Pardus ◽  
Semen Collection ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

scholarly journals Production and Quality of Semen in Boars in Insemination Centre

2017 ◽  
Vol 65 (4) ◽  
pp. 1189-1193
Author(s):  
Vendula Kamanová ◽  
Zdeněk Hadaš ◽  
Pavel Nevrkla
Keyword(s):  
Sperm Concentration ◽  
Semen Parameters ◽  
Volume Percentage ◽  
Semen Collection ◽  
Semen Volume ◽  
Concentration Levels ◽  
Age Category

The aim of this study was to analyze production and quality of semen in boars in an insemination centre. Experimental material consisted of 8,052 semen samples collected from 146 boars. During the period of 5 years, an analysis of semen parameters was conducted. The obtained data were divided according to age of boars at the time of semen collection. Results showed that boars between 8 months and 3 years of age reached higher levels of semen volume, percentage of abnormal spermatozoa and number of spermatozoa. Older boars had lower sperm concentration levels. The largest total number of spermatozoa and corrected number of spermatozoa were observed in boars between 13 and 36 months of age. Later, the production of spermatozoa decreased. Based on the results of the presented study, the age category of 13 – 36 months can be recommended to dominate in the herd of boars in the insemination centre.

Hormonal induction and semen characteristics of tambaqui Colossoma macropomum

Zygote ◽  
2011 ◽  
Vol 20 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Alexandre Nizio Maria ◽  
Hymerson Costa Azevedo ◽  
Jadson Pinheiro Santos ◽  
Paulo César Falanghe Carneiro
Keyword(s):  
Sperm Concentration ◽  
Fold Increase ◽  
Colossoma Macropomum ◽  
Morphological Abnormalities ◽  
Qualitative And Quantitative ◽  
Semen Collection ◽  
Hormonal Induction ◽  
Semen Volume ◽  
Abdominal Massage ◽  
Quantitative Properties

SummaryIn the hatchery-bred tambaqui Colossoma macropomum, spontaneous semen release does not occur, and hand-stripping produces reduced semen volume. The goal of this work is to evaluate the effects of hormonal induction with carp pituitary extract (CPE) on both qualitative (visual aspect, pH, motility, viability and morphological abnormalities) and quantitative (volume, concentration and number of spermatozoa per ejaculate) traits of tambaqui semen. Eleven males were treated with CPE (induced), and 11 were left untreated as a control (non-induced). All analysed parameters except motility and percentage of viable spermatozoa presented significant differences (p < 0.05) between the induced and non-induced treatments. CPE induction resulted in a 25-fold increase in semen volume and a 10-fold increase in the number of spermatozoa collected. However, both sperm concentration and the frequency of sperm with morphological abnormalities (commonly detached heads or bent tails) were significantly lower in CPE-induced fish. The hormonal induction of tambaqui males with CPE is efficient and positively influences some qualitative and quantitative properties of semen. Additionally, semen collection via gentle abdominal massage occurs more readily in CPE-induced fish.

scholarly journals The effect of Foot and Mouth disease on reproductive performance of Holstein bulls in Artificial Insemination Center of Iraq

2012 ◽  
Vol 36 (1) ◽  
pp. 1-7
Author(s):  
AL-Badry K. I.
Keyword(s):  
Reproductive Performance ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Foot And Mouth Disease ◽  
Reproductive Efficiency ◽  
Semen Collection ◽  
Semen Volume ◽  
Holstein Bulls ◽  
Disease Effect

This study was carried out in Artificial Insemination Center of Iraq to revealed FMD disease effect on some seminal attributer parameters of 14 imported Holstein bulls divided to three groups according to different reproductive efficiency (four High, five medium and five weak). Results showed that FMD disease had significant (P < 0.05) adverse effect on most seminal attributer parameters, mass, individual motility and sperm concentration / ml during post disease in first of two, four, all months of high, medium and weak semen quality bulls respectively .but semen volume didn’t influenced significantly with this disease. So semen collection should be suspended until resume normal fertility of sperm, after two, four month of high and medium bulls respectively, and must be revealed weak bulls when disease happen to avoid the failure of conception from artificial insemination and there is no economic benefit to use or keep weak bulls

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