scholarly journals Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

2021 ◽  
pp. 3156-3163
Author(s):  
Bongot Huaso Mulia ◽  
Ardyta Widianti ◽  
Jansen Manansang ◽  
Dedi Rahmat Setiadi ◽  
Vincentia Trisna Yoelinda ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Total Concentration ◽  
Sperm Concentration ◽  
Panthera Pardus ◽  
Semen Collection ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

2016 ◽  
Vol 28 (10) ◽  
pp. 1518 ◽  
Author(s):  
Urszula Marzec-Wróblewska ◽  
Piotr Kamiński ◽  
Paweł Łakota ◽  
Marek Szymański ◽  
Karolina Wasilow ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Human Semen ◽  
Glutathione Peroxidase Activity ◽  
Normal Sperm ◽  
Group I ◽  
Gpx Activity

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

scholarly journals Repeatability Estimates For Seminal Traits And Their Phenotypic Relationships With Testes Measurements And Performance Traits In Black Bengal Buck

1970 ◽  
Vol 37 (2) ◽  
pp. 34-41 ◽  
Author(s):  
AK Rajuana ◽  
MR Tayabur ◽  
MA Hoque ◽  
SS Husain ◽  
Z Sultana
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Body Condition ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Performance Traits ◽  
Mass Activity ◽  
And Performance

Repeatability for seminal traits and their phenotypic relationships with testes measurements and performance traits in Black Bengal bucks were estimated from a total of 116 repeated observations on 15 young Black Bengal bucks. Performance traits included age, body condition and body weight of bucks. Testes measurements were testes length, breadth and volume, and scrotal circumference, while seminal traits were ejaculate volume, semen density, mass activity, sperm motility, sperm concentration, total sperm per ejaculation and percent of normal sperm. High positive correlations (ranging from 0.81 to 0.90) were found between body weight and testes measurements. Semen volume and percent of normal sperm were positively correlated with age, body condition and body weight of bucks (ranging from 0.24 to 0.60). The testes measurements were strongly and positively correlated with semen volume and total sperm per ejaculation (ranging from 0.53 to 0.61), while the correlations between testes measurement and percent of normal sperm were moderate (ranging from 0.34 to 0.44). Among seminal traits, strong correlations were found between semen volume and total sperm per ejaculation (0.81) and, between mass activity and sperm motility (0.82). Repeatability of ejaculate volume was higher (r = 0.78) which indicated that selection or culling for semen ejaculate volume could be practiced from single or few observations. It could be concluded that bucks’ age, body weight and body condition along with testes volume should be considered as selection criteria for improving semen quality and semen production of breeding bucks. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9879 BJAS 2008; 37(2): 34-41

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

2020 ◽  
Vol 13 (5) ◽  
pp. 840-846
Author(s):  
R. Indriastuti ◽  
M. F. Ulum ◽  
R. I. Arifiantini ◽  
B. Purwantara
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Individual Variation ◽  
Sperm Concentration ◽  
Individual Factors ◽  
Abnormal Morphology ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls. Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen. Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.

scholarly journals Physical and morphological characteristics of the first three jets of Pêga jackasses sperm-rich fraction

2016 ◽  
Vol 68 (4) ◽  
pp. 845-852
Author(s):  
L.E. Carvalho ◽  
J.M. Silva Filho ◽  
M.S. Palhares ◽  
A.L.R. Sales ◽  
A.T. Gonczarowska ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Morphological Characteristics ◽  
Open Model ◽  
Cytoplasmic Droplet ◽  
The Third ◽  
Semen Collection ◽  
Normal Sperm ◽  
Artificial Vagina

ABSTRACT The first three jets of the sperm-rich fraction of Pêga jackasses were collected and assessed separately. Five fertile Pêga jackasses were used as semen donors and underwent fractionated semen collection, using an open model artificial vagina. The first three jets of the semen were collected separately and assessed for volume, sperm motility, vigor, concentration/mL of semen, and sperm morphology. These characteristics were compared between first, second and third jets and between jackasses. It was observed that the jet volume differed (P<0.05) between jackasses, although it was similar (P>0.05) between first, second and third jets. Sperm motility did not differ (P>0.05) between jets and jackasses. Vigor was similar (P>0.05) between jets of the same jackass, and only the first jet differed (P<0.05) between jackasses. The first, second and third jets of the sperm-rich fraction had decreased sperm concentrations (P<0.05) of 955.56, 725.56 and 280.56x 106 sperm/mL of semen, respectively. Sperm morphology differed between the first three jets only for the incidence of mid-piece defect, higher in the third one (4.26%), compared to the first (3.36%) and second (3.38%) ones. When comparing the morphological characteristics of the sperm-rich fraction between five jackasses, regardless of the jet, there were differences in the percentage of normal sperm, proximal cytoplasmic droplet, mid-piece and head defects.

scholarly journals Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol

2005 ◽  
Vol 57 (6) ◽  
pp. 764-771
Author(s):  
A.R. Silva ◽  
R.C.S. Cardoso ◽  
L.D.M. Silva
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Tris Buffer ◽  
Normal Sperm ◽  
Manual Stimulation

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were observed between treatments after thawing regarding sperm motility and vigor, normal sperm morphology rate or longevity. Both dilution rates can be efficiently used for canine semen freezing.

scholarly journals Study on buck evaluation based on semen quality and fertility

2013 ◽  
Vol 42 (2) ◽  
pp. 101-108 ◽  
Author(s):  
F Sultana ◽  
SS Husain ◽  
A Khatun ◽  
AS Apu ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Fertility Data ◽  
Three Stages ◽  
Live Sperm

The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B1, B2, B3, B4 and B5) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility, percentage of live sperm and percentage of normal sperm were ranged from 0.58±0.17 to 1.04±1.1 ml, 2827±0.76 to 3132

STUDY ON BODY WEIGHT AND SCROTAL CIRCUMFERENCE AND ITS CORRELATIONS WITH SEMEN PRODUCTION TRAITS IN CROSSBRED JERSEY BULLS

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
A. Gopinathan ◽  
S.N. Sivaselvam ◽  
S.M.K. Karthickeyan ◽  
R. Venkataramanan ◽  
K. Kulasekar
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Tamil Nadu ◽  
Sperm Concentration ◽  
The Body ◽  
Production Traits ◽  
Scrotal Circumference ◽  
Frozen Semen ◽  
Semen Volume ◽  
Mass Activity

The present study was aimed to investigate the effects of body weight and scrotal circumference on semen production traits of 136 crossbred Jersey bulls, which were maintained at three frozen semen stations of Tamil Nadu, India. Semen production traits such as semen volume, sperm concentration, mass activity, initial sperm motility, post-thaw motility and number of doses per ejaculate were taken for this study. The overall mean values for semen volume (ml), sperm concentration (millions per ml), mass activity (0 to 5 scale), initial sperm motility (per cent), postthaw motility (per cent) and number of doses per ejaculate were 4.18 ± 0.02, 1052.80 ± 7.26, 2.09 ± 0.07, 63.36 ± 0.00, 50.52 ± 0.00 and 215.50 ± 2.01 respectively. From this study, it revealed that the body weight and scrotal circumference of bulls significantly influenced all semen production traits except mass activity. Bulls having a body weight between 350 to 550 kg produced better quality semen and more number of frozen semen doses per ejaculate. When the scrotal circumference was >36 cm, majority of the semen production traits showed better values. The body weight was positively and significantly correlated with scrotal circumference, Semen volume and number of frozen semen doses produced per ejaculate. The scrotal circumference was positively and significantly correlated with semen volume, mass activity and number of doses per ejaculate; negatively and significantly correlated with initial sperm motiltiy.

scholarly journals Semen Production and Semen Quality of Mehsana Buffalo Breed Under Semiarid Climatic Conditions of Organized Semen Station in India

2021 ◽  
Author(s):  
Nikhil Shantilal Dangar ◽  
Balkrishna P Brahmkshtri ◽  
Niteen Deshmukh ◽  
Kamlesh Prajapati
Keyword(s):  
Sperm Motility ◽  
Linear Mixed Model ◽  
Environmental Changes ◽  
Semen Quality ◽  
Random Effect ◽  
Sperm Concentration ◽  
Season Of Birth ◽  
Production Traits ◽  
Semen Collection ◽  
Semen Volume

Abstract Semen production data comprising of 55071 ejaculates of 144 bulls from Mehsana buffalo breed was analysed. The traits under study were semen volume, sperm concentration, initial sperm motility, post-thaw sperm motility and number of semen doses per ejaculate. The objective of the present study was to assess the effect of various factors affecting semen production traits and measure the semen production potential of Mehsana buffalo bulls. Data collected of semen production traits were analysed using linear mixed model, including a random effect of bull along with fixed effect of various non-genetic factors like farm, ejaculate number, season of birth, period of birth, season of semen collection and period of semen collection. First ejaculation had higher semen volume and sperm concentration resulted in to higher number of semen doses but semen quality was better in second ejaculation. Season of birth of the bull was affecting semen quality traits. As the period of birth advances semen volume increases whereas sperm concentration decreases which reflected in persistent production of number of semen doses per ejaculate. Monsoon and summer were favorable seasons for semen collection because of higher sperm concentration which resulted in to higher semen doses per ejaculate. Additionally, Monsoon collected semen had highest volume. Hence, monsoon followed by summer season would be the favorable season for semen collection. Period of semen collection affecting all the semen production traits under study but it did not have specific trend which means managemental and environmental changes over the period have sizable influence on the traits. Results of the study will help to plan future managemental practices and breeding strategies to improve semen production traits.

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