scholarly journals Mapping of Echinacea-based food supplements on the Romania market and qualitative evaluation of the most commonly used products

2020 ◽  
Vol 93 (2) ◽  
pp. 111-123
Author(s):  
Ferencz Elek ◽  
Demeter Eszter ◽  
Király Rebeka ◽  
Vancea Szende ◽  
Urkon Melinda ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Caffeic Acid ◽  
Acid Content ◽  
Qualitative Evaluation ◽  
Hplc Method ◽  
Chromatographic Fingerprint ◽  
Common Component ◽  
Chicoric Acid ◽  
Tlc Analysis ◽  
Pharmaceutical Practice

Abstract The aim of the study was to explore dietary supplements containing Echinacea on the Romanian market and their qualitative characterization. The products available on the market were aggregated in 2018, through an electronic search based on the register of the Romanian Medicine Agency (Agenția Națională a Medicamentului şi a Dispozitivelor Medicale din România – ANMDMR) and the list of dietary supplements registered by the Ministry of Agriculture and Rural Development (Institutul Național de Cercetare-Dezvoltare pentru Bioresurse Alimentare – IBA București, Serviciul național pentru plante medicinale, aromatice și produse ale stupului). There are no Echinacea containing medicines registered in Romania. However, there are 58 dietary supplements in the register, 52% of which are mono-components, 29% contain other herbs, plant extract or vitamins, while 19% are registered as tea. The label of 80% of monocomponent products and 76% of multicomponent supplements contains insufficient information: the plant name, its used part and processing methods (grist, extract, quantity) are not clearly identified. Among the listed dietary supplements, the 12 most commonly used formulations in pharmaceutical practice were subjected to phytochemical chromatographic evaluation: TLC and/or HPLC analysis were used. Three of seven monocomponent products showed proper chromatographic fingerprint, by TLC analysis. One monocomponent sample did not have an adequate chromatographic fingerprint. The labelling of multicomponent products was not appropriate. The TLC test suggests that based on the resulting fingerprint they contain E. purpureae herba. However, due to the presence of other components, the TLC does not allow a clear conclusion regarding the exact composition of the products. The developed HPLC method enables quantification of the concentration of caffeic acid, chicoric acid, echinacoside, chlorogenic and caftaric acids mixture in dietary supplements. None of the tested products contained echinacoside, which is a specific component of E. angustifolia and E. pallida root. In our method, the quantification of caftaric acid is approximate, because it partially overlaps the chlorogenic acid, which is a common component of plant samples, but negligible in Echinacea sp. The tested dietary supplements have a caffeic acid content of 20-140 µg/g, a chicoric acid content of 0.19-2.64 mg/g; the mixture of chlorogenic and caftartic acid is about 0.23-2.07 mg/g.

scholarly journals Anti-diabetes Constituents in Leaves of Smallanthus sonchifolius

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Xiang Zheng ◽  
He Fan ◽  
Kang Ting-Guo ◽  
Dou De-Qiang ◽  
Gai Kuo ◽  
...  
Keyword(s):  
Quality Control ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Analytical Method ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Smallanthus Sonchifolius ◽  
Rp Hplc

The inhibitory effect of smallanthaditerpenic acids A, B, C and D previously isolated from leaves of Smallanthus sonchifolius (yacon) on α-glucosidase were examined and their IC50 were determined to be 0.48 mg/mL, 0.59 mg/mL, 1.00 mg/mL, and 1.17 mg/mL respectively. In addition, a rapid, reliable RP-HPLC method for the analysis of chlorogenic acid, caffeic acid, and smallanthaditerpenic acids A and C in yacon leaves was established, and the variation in their contents in leaves from plants cultivated in different places and collected at different times of the year were compared. The established analytical method for determining smallanthaditerpenic acids A and C, chlorogenic acid and caffeic acid presented good results and could be used as a method for the quality control of S. sonchifolius leaves.

Study on Active Ingredient of Blueberry Pomace in Fermentation by Lentinus edodes Hypha

2014 ◽  
Vol 926-930 ◽  
pp. 238-241
Author(s):  
Li Guo ◽  
Peng Wang
Keyword(s):  
Gallic Acid ◽  
Active Ingredient ◽  
Ellagic Acid ◽  
Acid Content ◽  
Hplc Method ◽  
Spectrophotometric Analysis ◽  
Anthocyanin Content ◽  
Lentinus Edodes ◽  
Flavonoid Content ◽  
Fermentation Products

The effect of fermentation by Lentinus edodes hypha on active ingredient of blueberry pomace was studied, and the changes of fermentation products with protein, flavonoid and anthocyanin content were evaluated by spectrophotometric analysis. The protein content decreased slowly, and the flavonoid content in the first 66 h increased the highest level 0.09 mg/g, the anthocyanin content decreased during the first fermentation process, then increased to 0.568 mg/g at 30 h. The fermentation product of ellagic acid and gallic acid content were analyzed by using HPLC Method, the ellagic acid content decreased, and the gallic acid content increased first and then decreased, reached the highest value for the 0.310 mg/g in the first 54 h.

scholarly journals Analysis and prognosis of bitter acids content in Czech hop varieties - year 2018 and long-term comparisons and trends

2019 ◽  
Vol 65 (1) ◽  
pp. 23-31
Author(s):  
Alexandr Mikyška ◽  
Marie Jurková
Keyword(s):  
Acid Content ◽  
Dry Matter ◽  
Hplc Method ◽  
Relative Content ◽  
Final Evaluation ◽  
Bitter Acids ◽  
Bitter Acid ◽  
The Stability ◽  
Good Agreement

This article presents the results of the harvest forecast of the alpha- and beta-bitter acids of the Czech hop varieties in the 2018 harvest, the results are discussed in relation to the long-term averages, the composition of the bitter acid analogs and the stability of the varietal characteristics. 180 hop samples were analyzed using EBC 7.7 (HPLC) method. The alpha-acid content of the most important Saaz variety (2.9% in dry matter) was identical to the 2017 harvest and 18% rel. lower than the 26-year average. The alpha-acid content of Sladek (5.0%), Premiant (4.8%), and Saaz Late (1.6%) varieties was significantly lower than the long-term average. The harvest of the hops of the Kazbek and Agnus varieties was close to the long-term average. The ratio of alpha- and beta-acids, the relative content of cohumulone and the relative content of colupulone were consistent with long-term averages for the tested varieties. It was confirmed, that the harvest forecast gives timely information on the alpha-acids content of the harvest, the results were in good agreement with the values of the final evaluation of all harvested hops samples.

Caffeoyl Quinic and Tartaric Acids and Flavonoids from Lapsana communis L. subsp. communis (Asteraceae)

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1090-1092 ◽  
Author(s):  
D. Fontanel ◽  
C. Galtier ◽  
C. Viel ◽  
A. Gueiffier
Keyword(s):  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Hydroxycinnamic Acids ◽  
First Report ◽  
Chicoric Acid ◽  
Caftaric Acid ◽  
Aerial Parts ◽  
Tartaric Acids ◽  
Dicaffeoylquinic Acid

Abstract Six hydroxycinnamic acids : caffeic acid, chlorogenic acid, 3,5-O-dicaffeoylquinic acid 2-O-caffeoyltartaric acid (caftaric acid) and 2,3-O-dicaffeoyltartaric acid (chicoric acid) have been isolated from Lapsana communis L. subsp. communis aerial parts. Among flavonoids, only isoquercitrin, luteolin and luteolin-7-O-β-glucuronide were identified. Except for chlorogenic acid, these com­ pounds represent the first report in Lapsana communis. Chicoric acid is the major phenylpropanoic constituent in this plant.

scholarly journals Quantitative Determination of Pregnanes from Aerial Parts of Caralluma Species Using HPLC-UV and Identification by LC-ESI-TOF

2011 ◽  
Vol 94 (5) ◽  
pp. 1383-1390
Author(s):  
Bharathi Avula ◽  
Yatin J Shukla ◽  
Yan-Hong Wang ◽  
Ikhlas A Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Plant Materials ◽  
Aerial Parts ◽  
Photodiode Array ◽  
Array Detection ◽  
Na Ions

Abstract An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1–5 and 3–15 μg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LC/MS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.

scholarly journals The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

2020 ◽  
Vol 142 (2) ◽  
pp. 379-399
Author(s):  
Agnieszka Szopa ◽  
Paweł Kubica ◽  
Łukasz Komsta ◽  
Aleksandra Walkowicz-Bożek ◽  
Halina Ekiert
Keyword(s):  
Caffeic Acid ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Culture Media ◽  
Hplc Method ◽  
Shoot Cultures ◽  
Growth Cycles ◽  
Methanolic Extracts ◽  
Aronia Melanocarpa ◽  
High Production

Abstract Agitated shoot cultures of two aronias, Aronia melanocarpa (Michx.) Elliott and Aronia arbutifolia (L.) Pers., were maintained on Murashige & Skoog medium (1 mg/l BA and 1 mg/l NAA), both with and without the addition of various biosynthetic precursors of phenolic acids and depsides (phenylalanine, cinnamic acid, benzoic acid and caffeic acid). Each substance was added in 5 concentrations (0.1–10 mmol/l), each concentration at two time points (at the beginning and on the 10th day of cultures). Twenty-four phenolic acids were determined in methanolic extracts of the biomasses collected after 20 days of growth cycles by means of HPLC method with DAD detection. The presence of seven compounds was confirmed in all the extracts—five depsides (neochlorogenic, chlorogenic, cryptochlorogenic, isochlorogenic and rosmarinic acids), and syringic and caffeic acids. The main metabolites in A. melanocarpa shoot extracts were isochlorogenic, chlorogenic and neochlorogenic acids (max. 249.88, 450.35, 192.16 mg/100 g DW). The main metabolites in A. arbutifolia shoot extracts were: chlorogenic, isochlorogenic and cryptochlorogenic acids (max. 361.60, 224.5, 526.2 mg/100 g DW). The largest total amounts of the compounds were confirmed in the cultures of both aronias after the addition of cinnamic acid (989.79 and 661.77 mg/100 g DW, respectively) and caffeic acid (854.99 and 1098.46 mg/100 g DW, respectively) at concentrations of 5 mmol/l on 10th day of growth cycles. These maximum amounts were 3.41, 3.42, 2.95 and 5.67 times higher, respectively, than in the control cultures. This is the first report documenting the high production of depsides in shoot cultures of black and red aronias after feeding with their biosynthetic precursors.

Improving biomass and chicoric acid content in hairy roots of Echinacea purpurea L.

Biologia ◽  
2019 ◽  
Vol 74 (8) ◽  
pp. 941-951
Author(s):  
Reza Sajadi Tabar ◽  
Ahmad Moieni ◽  
Sajad Rashidi Monfared
Keyword(s):  
Hairy Roots ◽  
Acid Content ◽  
Echinacea Purpurea ◽  
Chicoric Acid

scholarly journals Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

2011 ◽  
Vol 41 (No. 3) ◽  
pp. 105-111 ◽  
Author(s):  
R. Koprna ◽  
V. Kučera ◽  
O. Kolovrat ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Erucic Acid ◽  
Doubled Haploid ◽  
Acid Content ◽  
Seed Quality ◽  
Quality Parameters ◽  
Hplc Method ◽  
Hybrid Breeding ◽  
Erucic Acid Content ◽  
Glucosinolate Content ◽  
Brassica Napus L

Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different “00” quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.  

scholarly journals Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

2014 ◽  
Vol 27 (2) ◽  
pp. 108-110
Author(s):  
Karolina Zapala ◽  
Grazyna Szymczak ◽  
Magdalena Wojciak-Kosior
Keyword(s):  
Caffeic Acid ◽  
Bioactive Compound ◽  
Reversed Phase ◽  
Hplc Method ◽  
Black Cohosh ◽  
Cimicifuga Racemosa ◽  
Medicinal Use ◽  
Relative Standard ◽  
Validation Parameters ◽  
History Of

Abstract Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 μg·g-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 μg·g-1).

scholarly journals Identification and Determination of Seven Phenolic Acids in Brazilian Green Propolis by UPLC-ESI-QTOF-MS and HPLC

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1791 ◽  
Author(s):  
Shengwei Sun ◽  
Meijuan Liu ◽  
Jian He ◽  
Kunping Li ◽  
Xuguang Zhang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Hplc Method ◽  
Operating Conditions ◽  
Total Phenolic ◽  
Artepillin C ◽  
Brazilian Green Propolis

Brazilian green propolis is a complex mixture of natural compounds that is difficult to analyze and standardize; as a result, controlling its quality is challenging. In this study, we used the positive and negative modes of ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time of flight mass spectrometry in conjunction with high-performance liquid chromatography for the identification and characterization of seven phenolic acid compounds in Brazilian green propolis. The optimal operating conditions for the electrospray ionization source were capillary voltage of 3500 V and drying and sheath gas temperatures of 320 °C and 350 °C, respectively. Drying and sheath gas flows were set to 8 L/min and 11 L/min, respectively. Brazilian green propolis was separated using the HPLC method, with chromatograms for samples and standards measured at 310 nm. UPLC-ESI-QTOF-MS was used to identify the following phenolic compounds: Chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, caffeic acid phenethyl ester (CAPE), and artepillin C. Using a methodologically validated HPLC method, the seven identified phenolic acids were then quantified among different Brazilian green propolis. Results indicated that there were no significant differences in the content of a given phenolic acid across different Brazilian green propolis samples, owing to the same plant resin sources for each sample. Isochlorogenic acid B had the lowest content (0.08 ± 0.04) across all tested Brazilian green propolis samples, while the artepillin C levels were the highest (2.48 ± 0.94). The total phenolic acid content across Brazilian green propolis samples ranged from 2.14–9.32%. Notably, artepillin C quantification is an important factor in determining the quality index of Brazilian green propolis; importantly, it has potential as a chemical marker for the development of better quality control methods for Brazilian green propolis.

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