scholarly journals Recent progress for capillary electrophoresis with electrochemical detection

2012 ◽  
Vol 10 (3) ◽  
pp. 639-651 ◽  
Author(s):  
Huan Yu ◽  
Xiaoyu Xu ◽  
Jinying Sun ◽  
Tianyan You
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
Recent Progress ◽  
Low Cost ◽  
High Sensitivity ◽  
Separation Performance ◽  
Separation Science ◽  
Short Analysis Time ◽  
High Separation ◽  
Important Progress

AbstractCapillary electrophoresis (CE) is an attractive technique in separation science because of its high separation performance, short analysis time and low cost. Electrochemical detection (EC) is a powerful tool for CE because of its high sensitivity. In this review, developments of CE-EC from 2008 to August, 2011 are reviewed. We choose papers of innovative and novel results to demonstrate the newest and most important progress in CE-EC.

scholarly journals Optimization of Mordenite Membranes Using Sucrose Precursor for Pervaporation of Water-Ethanol Mixtures

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 160
Author(s):  
Abdulaziz A. Alomair ◽  
Yousef Alqaheem
Keyword(s):  
Pyrolysis Temperature ◽  
Operating Temperature ◽  
Selective Adsorption ◽  
Sucrose Concentration ◽  
Low Cost ◽  
Water Flux ◽  
Separation Performance ◽  
Carbon Membranes ◽  
Adsorption Of Water ◽  
High Separation

Post-treated mordenite membranes were prepared using sucrose (C12H22O11) as a carbon precursor to block any pinholes and defects in the zeolite layer. The pervaporation (PV) process was used to separate ethanol from the water. The effects of the sucrose concentration and the pyrolysis temperature (650–850 °C) were investigated, and the resulting high separation performance compared to those post/pre-treated membranes was reported in the literature. In this study, mordenite carbon membranes yielded a water/ethanol separation factor of 990.37 at a water flux of 9.10 g/m2h. The influence of the operating temperature on the performance of the membrane also was considered. It was concluded that the selective adsorption of water through zeolite pores was achieved. The entire preparation procedure was achieved using a rapid, low-cost preparation process.

Simultaneous determination of whitening agents and parabens in cosmetic products by capillary electrophoresis with on-line sweeping enhancement

2014 ◽  
Vol 6 (19) ◽  
pp. 7615-7620 ◽  
Author(s):  
I-Chi Tsai ◽  
Chia-Yu Su ◽  
Cho-Chun Hu ◽  
Tai-Chia Chiu
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Separation Efficiency ◽  
Analysis Time ◽  
Cosmetic Products ◽  
Short Analysis Time ◽  
On Line ◽  
High Separation

An on-line sweeping-MEKC method for simultaneous determination of whitening agents and parabens in commercial cosmetic products was demonstrated. This approach was shown to offer high separation efficiency, short analysis time, and convenience of analysis. The LODs of the analytes were in the range from 8 to 162 nM (1.1 to 21.0 ng mL−1) and a 46 to 279-fold enhancement was achieved.

scholarly journals Review of recent developments of on-line sample stacking techniques and their application in capillary electrophoresis

2012 ◽  
Vol 10 (3) ◽  
pp. 611-638 ◽  
Author(s):  
Yonglei Chen ◽  
Wenjuan Lü ◽  
Xingguo Chen ◽  
Min Teng
Keyword(s):  
Capillary Electrophoresis ◽  
Separation Efficiency ◽  
Solid Phase ◽  
Low Cost ◽  
Optical Path Length ◽  
Separation Science ◽  
Sample Stacking ◽  
Recent Developments ◽  
On Line ◽  
Line Sample

AbstractCapillary electrophoresis (CE) has become one of the most useful tools in separation science because of its high separation efficiency, low cost, versatility, ease of sample preparation and automation. However, some limitations of CE, such as poor concentration sensitivity due to its lower sample loading and shorter optical path length, limits its further applications in separation science. In order to solve this problem, various on-line sample preconcentration techniques such as transient isotachophoresis preconcentration, field-enhanced sample stacking, micelle to solvent stacking, micelle collapse, dynamic pH junction, sweeping, solid phase extraction, single drop microextraction and liquid phase microextraction have been combined with CE. Recent developments, applications and some variants together with different combinations of these techniques integrating in CE are reviewed here and our discussions will be confined to the past three years (2008–2011).

scholarly journals Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020 ◽  
Author(s):  
Brae Petersen ◽  
Luke Gallion ◽  
Nancy Allbritton
Keyword(s):  
Capillary Electrophoresis ◽  
Open Source ◽  
Dynamic Range ◽  
Low Cost ◽  
Single Cells ◽  
Detection Limits ◽  
High Sensitivity ◽  
Small Sample ◽  
Fluorescence Detector ◽  
Silicon Photomultipliers

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.<br>

scholarly journals Recent Progress in Fiber Optofluidic Lasing and Sensing

2021 ◽  
Vol 11 (2) ◽  
pp. 262-278
Author(s):  
Xi Yang ◽  
Chaoyang Gong ◽  
Yiling Liu ◽  
Yunjiang Rao ◽  
Mateusz Smietana ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Recent Progress ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Gain Medium ◽  
High Signal ◽  
Biomedical Analysis ◽  
Sensing Applications

AbstractFiber optofluidic laser (FOFL) integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications. FOFLs not only inherit the advantages of lasers such as high sensitivity, high signal-to-noise ratio, and narrow linewidth, but also hold the unique features of optical fiber, including ease of integration, high repeatability, and low cost. With the development of new fiber structures and fabrication technologies, FOFLs become an important branch of optical fiber sensors, especially for application in biochemical detection. In this paper, the recent progress on FOFL is reviewed. We focuse mainly on the optical fiber resonators, gain medium, and the emerging sensing applications. The prospects for FOFL are also discussed. We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

2020 ◽  
Author(s):  
Brae Petersen ◽  
Luke Gallion ◽  
Nancy Allbritton
Keyword(s):  
Capillary Electrophoresis ◽  
Open Source ◽  
Dynamic Range ◽  
Low Cost ◽  
Single Cells ◽  
Detection Limits ◽  
High Sensitivity ◽  
Small Sample ◽  
Fluorescence Detector ◽  
Silicon Photomultipliers

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.<br>

Validation of the denaturing capillary electrophoresis (DCE) assay for non-invasive liquid biopsy in lung carcinoma: A study of concordance with the cobas EGFR mutation test v2.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23040-e23040
Author(s):  
Marek Minarik ◽  
Barbora Belsanova ◽  
Ondrej Fiala ◽  
Milos Pesek ◽  
Lucie Benesova
Keyword(s):  
Capillary Electrophoresis ◽  
Liquid Biopsy ◽  
Egfr Mutation ◽  
Low Cost ◽  
Stage Iv ◽  
High Sensitivity ◽  
Circulating Tumor Dna ◽  
Plasma Samples ◽  
Iso 15189 ◽  
Lung Cancer Patients

e23040 Background: The detection of circulating tumor DNA (ctDNA), referred to as “liquid biopsy” is becoming a preferred option for some patients with lung adenocarcinomas. The key for a suitable technology is high sensitivity enabling to detect small fractions of mutated alleles, the method has to be solid against false-positives. The aim of this work was to compare our method based on capillary electrophoresis under denaturing conditions (DCE) to FDA and CE IVD approved commercial kit - cobas EGFR Mutation Test v2. Methods: A total of 44 plasma samples were acquired from patients with clinicaly confirmed stage IV lung adenocarcinoma. There were samples from patients prior to receiving therapy (with a known tissue EGFR genotype) as well as several ones showing progression on a previously administered antiEGFR-therapy (with no tissue info available at the time of plasma sampling). DNA was isolated from multiple 2 mL aliquots of each plasma. The first aliquot was run on cobas z480 real-time instrument using unmodified manufacturer's protocol. Another 2ml aliquot was run by DCE assay (accredited method under ISO 15189:2012), which is based on a PCR followed by a fragment analysis on an ABI capillary analyzer (Sanger sequencer). Results: The mutual concordance was 84.1% (37/44). cobas assay identified 11 EGFR-positive plasma samples (6 x Ex19del, 3 x L858R, 1x T790M combined with Ex19del and 1x T790M combined with L858R). DCE identified 8 EGFR-positive samples (6 x Ex19del, 1 x L858R, 1xT790M combined with L858R). DCE missed 5 of cobas-positive samples (2x Ex19del, 2x L858R and 1x T790M) while in turn identifying 2x Ex19del plasma samples that were concordant with tissue, but missed by cobas. Overall concordance with tissue was 73.3% (22/30) for cobas, 70.0% (21/30) for DCE assay and 80.0% (24/30) for both combined. Conclusions: DCE approach is reliable for low levels of mutations in plasma of lung cancer patients and comparable (and complementary) to cobas. Its advantage is simplicity, low cost and a universal, straighforward adaptability to virtually any mutation at any site allowing to detect oncogenic as well as tumor supressor mutations in ctDNA. Supported by grant 17-30748A.

scholarly journals A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Thoriq Salafi ◽  
Yi Zhang ◽  
Yong Zhang
Keyword(s):  
Recent Progress ◽  
Lateral Displacement ◽  
Wide Spectrum ◽  
Point Of Care ◽  
Low Cost ◽  
Particle Separation ◽  
Medical Diagnostics ◽  
Separation Performance ◽  
Deterministic Lateral Displacement ◽  
Biomolecules Detection

Abstract The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics. In this field, microfluidic deterministic lateral displacement (DLD) holds a promise due to the ability of continuous separation of particles by size, shape, deformability, and electrical properties with high resolution. DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes. DLD techniques have been previously reviewed in 2014. Since then, the field has matured as several physics of DLD have been updated, new phenomena have been discovered, and various designs have been presented to achieve a higher separation performance and throughput. Furthermore, some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection. This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection. Furthermore, current challenges and potential solutions of DLD are also discussed. We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications. In particular, the rapid, low-cost, and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.

scholarly journals A review of biomass materials for advanced lithium–sulfur batteries

2019 ◽  
Vol 10 (32) ◽  
pp. 7484-7495 ◽  
Author(s):  
Huadong Yuan ◽  
Tiefeng Liu ◽  
Yujing Liu ◽  
Jianwei Nai ◽  
Yao Wang ◽  
...  
Keyword(s):  
Recent Progress ◽  
Low Cost ◽  
High Abundance ◽  
Chemical Adsorption ◽  
Environmental Friendliness ◽  
Lithium Sulfur Batteries ◽  
Biomass Materials ◽  
Physical And Chemical ◽  
Lithium Sulfur

This review summarizes recent progress of biomass-derived materials in Li–S batteries. These materials are promising due to their advantages including strong physical and chemical adsorption, high abundance, low cost, and environmental friendliness.

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