Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)The aim of this study was to investigate the effect of clomazone herbicide on oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes inin vitroconditions. The activity of catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE), as well as the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in human erythrocytes exposed (in vitro) to clomazone at varying concentrations in the range of 0, 100, 250 and 500 μg/L for 1 h at 37°C. TBARS levels were significantly higher in erythrocytes incubated with clomazone at 100, 250 and 500 μg/L. However, erythrocyte CAT and AChE activities were decreased at all concentrations tested. SOD activity was increased only at 100 μg/L of clomazone. GSH levels did not change with clomazone exposure. These results clearly showed clomazone to induce oxidative stress and AChE inhibition in human erythrocytes (in vitro). We, thus, suggest a possible role of ROS on toxicity mechanism induced by clomazone in humans.

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Acrylamide-induced oxidative stress in human erythrocytes

Human & Experimental Toxicology ◽

10.1177/0960327109350664 ◽

2009 ◽

Vol 28 (10) ◽

pp. 611-617 ◽

Cited By ~ 30

Author(s):

Betul Catalgol ◽

Gül Özhan ◽

Buket Alpertunga

Keyword(s):

Oxidative Stress ◽

Reduced Glutathione ◽

Human Erythrocytes ◽

Antioxidant Enzyme Activities ◽

Total Glutathione ◽

Sod Activity ◽

Spectrophotometric Methods ◽

Low Concentrations ◽

Different Doses

Acrylamide (AA), a widely used industrial chemical, is shown to be neurotoxic, mutagenic and carcinogenic. This study was carried out to investigate the effects of different doses of AA on lipid peroxidation (LPO), haemolysis, methaemoglobin (MetHb) and antioxidant system in human erythrocytes in vitro. Erythrocyte solutions were incubated with 0.10, 0.25, 0.50 and 1.00 mM of AA at 37°C for 1 hour. At the end of the incubation, malondialdehyde (MDA), an end product of LPO, was determined by liquid chromatography (LC) while total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes and the rates of haemolysis and MetHb were determined by spectrophotometric methods. All of the studied concentrations of AA increased MetHb formation and SOD activity, and induced MDA formation and haemolysis due to the destruction of erythrocyte cell membrane. AA caused a decrease in the activities of GSH-Px, CAT and GSH levels. However, these effects of AA were seen only at higher concentrations than AA intake estimated for populations in many countries. We suggest that LPO process may not be involved in the toxic effects of AA in low concentrations, although the present results showed that the studied concentrations of AA exert deteriorating effects on antioxidant enzyme activities, LPO process and haemolysis.

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Moderate physical activity reduces 10-year diabetes incidence: the mediating role of oxidative stress biomarkers

International Journal of Public Health ◽

10.1007/s00038-017-1052-8 ◽

2017 ◽

Vol 63 (2) ◽

pp. 297-305 ◽

Cited By ~ 3

Author(s):

Efi Koloverou ◽

◽

Konstantinos Tambalis ◽

Demosthenes B. Panagiotakos ◽

Ekavi Georgousopoulou ◽

...

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Moderate Physical Activity ◽

Diabetes Incidence ◽

Oxidative Stress Biomarkers ◽

Mediating Role ◽

Stress Biomarkers

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Corrigendum to ‘Predictors of Outcome in Spontaneous Intracerebral Hemorrhage—Role of Oxidative Stress Biomarkers’ [World Neurosurgery 120 (2018) 601]

World Neurosurgery ◽

10.1016/j.wneu.2019.02.024 ◽

2019 ◽

Vol 125 ◽

pp. 559

Author(s):

Ajay Hegde ◽

G. Lakshmi Prasad ◽

Shwethapriya Rao

Keyword(s):

Oxidative Stress ◽

Intracerebral Hemorrhage ◽

Spontaneous Intracerebral Hemorrhage ◽

Predictors Of Outcome ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers

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Predictive Role of F2-Isoprostanes as Biomarkers for Brain Damage after Neonatal Surgery

Disease Markers ◽

10.1155/2017/2728103 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

L. J. Stolwijk ◽

P. M. A. Lemmers ◽

M. Y. A. van Herwaarden ◽

D. C. van der Zee ◽

F. van Bel ◽

...

Keyword(s):

Oxidative Stress ◽

Risk Factors ◽

Brain Injury ◽

Multivariable Analysis ◽

Major Surgery ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Significant Difference ◽

Predictive Role

Objective. Neonates have a high risk of oxidative stress during anesthetic procedures. The predictive role of oxidative stress biomarkers on the occurrence of brain injury in the perioperative period has not been reported before. Methods. A prospective cohort study of patients requiring major surgery in the neonatal period was conducted. Biomarker levels of nonprotein-bound iron (NPBI) in plasma and F2-isoprostane in plasma and urine before and after surgical intervention were determined. Brain injury was assessed using postoperative MRI. Results. In total, 61 neonates were included, median gestational age at 39 weeks (range 31–42) and weight at 3000 grams (1400–4400). Mild to moderate brain lesions were found in 66%. Logistic regression analysis showed a significant difference between plasma NPBI in patients with nonparenchymal injury versus no brain injury: 1.34 umol/L was identified as correlation threshold for nonparenchymal injury (sensitivity 67%, specificity 91%). In the multivariable analysis, correcting for GA, no other significant relation was found with the oxidative stress biomarkers and risk factors. Conclusion. Oxidative stress seems to occur during anaesthesia in this cohort of neonates. Plasma nonprotein-bound iron showed to be associated with nonparenchymal injury after surgery, with values of 1.34 umol/L or higher. Risk factors should be elucidated in a more homogeneous patient group.

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Modulation ofN-methyl-d-aspartate receptors in isolated rat heart

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2017-0056 ◽

2017 ◽

Vol 95 (11) ◽

pp. 1327-1334 ◽

Cited By ~ 5

Author(s):

Ivan Srejovic ◽

Vladimir Zivkovic ◽

Tamara Nikolic ◽

Nevena Jeremic ◽

Isidora Stojic ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Heart ◽

Isolated Rat Heart ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Combined Application ◽

Wistar Albino Rats ◽

Isolated Rat

Considering the limited data on the role of NMDA-Rs in the cardiovascular system, the aim of the present study was to examine the effects of NMDA and DL-Hcy TLHC, alone and in combination with glycine, memantine, and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance for 5 min, followed by a washout period of 10 min. Using a sensor placed in the left ventricle, we registered the following parameters of myocardial function: dp/dtmax, dp/dtmin, SLVP, DVLP, HR; CF was measured using flowmetry). We estimated the following oxidative stress biomarkers in the coronary venous effluent using spectrophotometry: TBARS, NO2−, O2−, and H2O2. NMDA alone did not induce any change in any of the observed parameters, while DL-Hcy TLHC alone, as well as a combined application of NMDA and DL-Hcy TLHC with glycine, induced a reduction of most cardiodynamic parameters. Memantine and ifenprodil induced a reduction of cardiodynamic parameters and CF, as well as some oxidative stress biomarkers.

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