scholarly journals ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta

2017 ◽  
Vol 1 (1) ◽  
pp. 16
Author(s):  
I Made Dira Swantara ◽  
Wiwik Susanah Rita ◽  
Rr Anisa Hernindy
Keyword(s):  
Stationary Phase ◽  
Anticancer Activity ◽  
Silica Gel ◽  
Screening Test ◽  
Mobile Phase ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Toxicity Screening ◽  
Polyphenol Compounds ◽  
Thousand Islands

AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The anticancer isolate contained alkaloid, steroid, and polyphenol compounds

scholarly journals UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Ni Made Dwi Desiyanti ◽  
I Made Dira Swantara ◽  
I Putu Sudiarta
Keyword(s):  
Ir Spectra ◽  
Silica Gel ◽  
Column Chromatography ◽  
Myzus Persicae ◽  
Mobile Phase ◽  
Ethanol Extract ◽  
Annona Muricata ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Metabolite Extraction

The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.

scholarly journals Chemical Constituents of the Insecticidal Active Extract of Tinospora crispa

2019 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Nor Aziyah Bakhari ◽  
Siti Nur Amirah Diana Fadzillah ◽  
Norain Isa
Keyword(s):  
Blood Pressure ◽  
Petroleum Ether ◽  
Silica Gel ◽  
1H Nmr ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
First Time

Tinospora crispa Miers (Menispermaceae) is a climbing vine with stems rich in warts. The plant is called Akar Seruntum or Patawali in Malaysia and is widely used for treating skin complaints, malaria, bacterial abscess, high blood pressure and diabetes. In the present study, the stems of T. crispa were collected from the locality and succesively extracted with petroleum ether, followed by chloroform and ethanol. The insecticidal active extract (ethanol extract) was  subjected to column chromatography of silica gel eluted with a gradient mobile phase containing hexane, chloroform and ethanol. Among the chemical constituents isolated are n-tetracosyl trans-ferulate and n-octacosyl alcohol, along with three known aporphine alkaloids; N-formylnornuciferine, N-acetylnornuciferine and lysicamine. All compounds were identified by comparing their spectroscopic data (UV, IR, 1H NMR, MS) with data from corresponding values in the literature. Isolation of n-tetracosyl trans-ferulate and n-octacosyl alcohol is reported the first time for T. crispa.

Cellulose Tris(3,5-Dimethylphenylcarbamate) Regioselectively Bonded to Small Pore Silica Gel as Chiral Stationary Phase for HPLC

2011 ◽  
Vol 117-119 ◽  
pp. 1361-1364
Author(s):  
Yi Jun Zhang ◽  
Cai Xia Dong ◽  
Jun Chen ◽  
Run Qiang Liu
Keyword(s):  
Stationary Phase ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
High Performance Liquid Chromatographic ◽  
Hydroxyl Group ◽  
Cellulose Derivatives ◽  
Terephthaloyl Chloride ◽  
Small Pore

A bifunctional reagent of terephthaloyl chloride was initially adopted as a spacer reagent to prepare the bonded types of chiral stationary phase (CSP) with cellulose derivatives. (3,5-dimethylphenyl)carbamates of cellulose (CDMPC) regioselectively bonded to small pore (3-aminopropyl)silica gel (APS) were prepared with terephthaloyl chloride as a spacer at the 6-position of the primary hydroxyl group on the glucose unit of cellulose. Enantioseparations of five racemic samples are evaluated on the prepared CSP under normal-phase high-performance liquid chromatographic mode with hexane- isopropylalcohol as the mobile phase. The influence of flow rates and mobile phase compositions on the resolution were investigated. The prepared stationary phase was exhibited an effective chiral recognition.

scholarly journals Aktivitas Antelmintik Subfraksi dariFraksi Etanol Daun Pugun Tanoh [Picria fel-teraae (Lour.)]

2018 ◽  
Vol 1 (3) ◽  
pp. 111-113
Author(s):  
Popi Patilaya ◽  
Dadang Irfan Husori ◽  
Henny Sri Wahyuni
Keyword(s):  
Stationary Phase ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Ascaris Lumbricoides ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Vacuum Liquid Chromatography ◽  
Parasitic Worms ◽  
Layer Chromatography ◽  
Ethanol Fraction

Pugun tanoh [Picria fel-terrae (Lour.)] merupakan salah satu tumbuhan obat Indonesia yang memiliki potensi sebagai antelmintik. Ekstrak etanol daun pugun tanoh dan fraksi-fraksinya mampu membunuh cacing parasit Ascaris lumbricoides dan Ascaridia gali. Pengujian terhadap aktivitas antelmintik subfraksi dari ekstrak etanol tersebut perlu dilakukan sebagai upaya untuk memperoleh senyawa bioaktifnya. Penelitian ini bertujuan untuk mengetahui aktivitas antelmintik subfraksi dari fraksi etanol daun pugun tanoh. Penelitian dilakukan dengan memfraksinasi 20g fraksi etanol daun pugun tanoh secara kromatografi cair vakum dengan fase gerak landaian n-heksana-etil asetat dan etil asetat-metanol menggunakan fase diam silika gel 60H. Setiap 250 ml cuplikan ditampung dan ditentukan pola kromatogramnya dengan kromatografi lapis tipis menggunakan fase gerak n-heksana-etilasetat (70:30) dan fase diam silika gel GF254. Cuplikan dengan pola kromatografi yang sama dikumpulkan sebagai satu subfraksi. Setelah diuapkan, subfraksi diuji aktivitas antelmintiknya terhadap Pheretima posthuma.Hasil penelitian menunjukkan bahwa fraksi etanol daun pugun tanoh menghasilkan 4 subfraksi yaitu SF1 (0,10%), SF2 (4,00%), SF3 (4,05%), dan SF4 (73,55%). Waktu kematian P. posthuma akibat paparan SF1, SF2, SF3, dan SF4 masing-masing adalah 77,00 ± 1,00 menit; 56,33 ± 1,76 menit; 79,33 ± 1,33 menit; dan 112,33 ± 0,67 menit. Subfraksi dari fraksi etanol daun pugun tanoh memiliki aktivitas antelmintik dimana SF2 lebih kuat dibandingkan SF3, SF1, dan SF4.   Pugun tanoh [Picria fel-terrae (Lour.)] is one of the Indonesian medicinal plants which has the potential as an anthelmintic. Ethanol extract of pugun tanoh leaves and their fractions were able to destroy the parasitic worms Ascaris lumbricoides and Ascaridia. The evaluation of antelmintic activity of subfraction of ethanol extract needs to be done in an effort to obtain its bioactive compounds. This study aimed to determine the anthelmintic activity of subfraction of  pugun tanoh leavesethanol fraction. The study was carried out by fractionating 20g ethanol fraction of pugun tanoh leaves by vacuum liquid chromatography with a mobile phase of n-hexane-ethyl acetate and ethyl acetate-methanol using the stationary phase of silica gel 60H.Each 250 ml aliquot was collected and the chromatogram pattern was determined by thin layer chromatography using the n-hexane-ethylacetate(70:30)  asmobile phase and silica gel GF254 as stationary phase. The Aliquots with same chromatographic pattern were collected as one subfraction. After being evaporated, the subfraction was tested for its anthelmintic activity against Pheretima posthuma. The results showed that ethanol fraction of pugun tanoh leaves produced 4 subfractions namely SF1 (0.10%), SF2 (4.00%), SF3 (4.05%), and SF4 (73.55%). The time of P. posthuma's death due to exposure to SF1, SF2, SF3, and SF4 was 77.00 ± 1.00; 56.33 ± 1.76; 79.33 ± 1.33; and 112.33 ± 0.67 minutes, respectively. Subfraction of ethanol fraction of pugun tanoh leaves has anthelmintic activity in which SF2 was stronger than SF3, SF1, and SF4

scholarly journals Molluscicidal Potential of Column Purified Fractions of Allium cepa against Intermediate Host of Urinary Schistosomiasis (Bulinus globosus) in Sokoto, Nigeria

2018 ◽  
pp. 1-6
Author(s):  
J. Suleiman ◽  
K. Singh ◽  
A. Y. Bala ◽  
M. T. Muhammad ◽  
M. S. Yakubu
Keyword(s):  
Stationary Phase ◽  
Ethyl Acetate ◽  
Intermediate Host ◽  
Laboratory Condition ◽  
Silica Gel ◽  
Allium Cepa ◽  
Mobile Phase ◽  
Intermediate Hosts ◽  
Urinary Schistosomiasis ◽  
Bulinus Globosus

Potential of column purified fractions of Allium cepa bulb against intermediate hosts of urinary schistosomiasis (Bulinus globosus) was conducted in laboratory condition. The fresh bulbs of A. cepa were purchased from Ramin Kura market of Sokoto, identified and authenticated by a taxonomist. The bulbs were sliced into pieces, air dried and powdered. Extracts were obtained using methanol as polar then purified with silica gel as a stationary phase while N-hexane and ethyl acetate (1:1) as the mobile phase. Thirteen fractions each fraction containing 10 ml of the effluent was collected, the collected extracts were left open for evaporation for 48 hours. Ten adult B. globosus were immersed in 3liters of water containing different concentrations of the fraction and each treatment was replicated three times with control in the same condition without treatment, observations were recorded after 24 hours up to 96 hours. The toxicity experiment showed that fractions (F7, F8, F6 and F9) were most toxic fractions, LC50 after 96 hours was 19.371 mg/l. based on findings from this research it can be concluded that, A. Cepa was very potent and can be used for control of B. globosus in order to prevent urinary schistosomiasis infection in endemic areas and drugs industries may use the extracts of these plants for production of molluscicides.

scholarly journals Polyaniline modified silica gel coupled with green solvent as eco favourable mobile phase in thin layer chromatographic analysis of organic dyes

2021 ◽  
Vol 4 (1) ◽  
pp. 41
Author(s):  
Mahfoozurrahman Khan ◽  
Ali Mohammad ◽  
Qasim Ullah ◽  
Faiz Mohammad
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Silica Gel ◽  
Mobile Phase ◽  
Organic Dyes ◽  
Thymol Blue ◽  
Transmission Electron Micrograph ◽  
Chromatographic System ◽  
Modified Silica ◽  
Modified Silica Gel

This article studies a new green eco-friendly TLC (thin layer chromatography) using silica gel and polyaniline modified silica gel as stationary phase in combination with ethyl acetate (EA), n-butyl acetate (BA) and butane-1-ol (BO) solutions as mobile phase for the comparative study of migration behaviour of organic dyes to identify the most suitable thin layer chromatographic system for the resolution of co-existing dyes. Better separation efficiency was observed by modifying silica gel with polyaniline as compared to pure silica stationary phase. Densitogrpahic presentation of separations achieved on polyaniline modified silica gel Pani@SG-EB1 was also presented. The thin layer chromatographic system comprising of polyaniline modified silica gel Pani@SG-EB1 as stationary phase and n-butyl acetate:DDW, 5:5 as green mobile phase was observed to be the most favourable for the separation of various combinations of three or four-component mixtures of organic dyes viz. methyl thymol blue, tartrazine, carmoisine, rose bengal, amidoblack 10B, bromopyrogallol red and 4-nitrobenzene dizonium tetrafluoroborate. The effect of presence of cations and anions on separation trend was also examined and the limits of detection of the separated organic dyes were estimated. Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron micrograph (TEM) studies were undertaken to characterize silica gel and modified silica gel (stationary phase). The developed method has been successfully applied for the identification of carmoisine in Solvin cold DS syrup and tartrazine in MefastTM syrup.

scholarly journals ISOLATION AND IDENTIFICATION OF MAIN AND SIDE PRODUCTS FROM ADDITION REACTION OF CARYOPHYLLENE OXIDE BY FORMIC ACID

2010 ◽  
Vol 2 (3) ◽  
pp. 155-160
Author(s):  
M Muchalal ◽  
Trisulistyaningsih Rahayu
Keyword(s):  
Gas Chromatography ◽  
Stationary Phase ◽  
Formic Acid ◽  
Silica Gel ◽  
Mobile Phase ◽  
Main Product ◽  
Addition Reaction ◽  
Caryophyllene Oxide ◽  
Isolation And Identification ◽  
Infrared Spectrophotometer

Main and side products from reaction of caryophyllene oxide and formic acid have been isolated using column chromatography with silica gel 60 G as stationary phase and n-hexane : ethyl acetat (9:1, v/v) as mobile phase. The first and second fraction were analyzed using infrared spectrophotometer, gas chromatography, and gas chromatography-mass spectrometer. Caryophyllene formiate as a main product was found in the first fraction. The side product that had been identified were 4,10,10-trimethyl-7-methylenebicyclo[6,2,0]decane-4-carbaldehyde, 2-(9-hydroxy-4,4,8 trimethyltricyclo[6,3,1,01,5]dodecyl)metanoate, clovanediol,4,11,11-trimethyl-8 methylene-3-bicyclo-[7,2,0]-undecen-5-ol,2,5-(6,10,10 imethyltricyclo[7,2,1,01,9]dodecyl)metanedioat, and derivative of caryophylleneformiate.   Keywords: Isolation, identification, caryophyllene oxide

scholarly journals OPTIMATION OF ETHANOL CONCENTRATION IN EXTRACTION OF EUGENOL FROM GALANGAL RHIZOME

2017 ◽  
Vol 10 (14) ◽  
pp. 18
Author(s):  
Adi Yugatama ◽  
Nur Wahida Ardiyati ◽  
Ira Yulianti
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Thin Layer ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Ethanol Concentration ◽  
Ethanol Extract ◽  
Standard Curve ◽  
Layer Chromatography ◽  
Curve Equation

   Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F254 as stationary phase and applying a wavelength of 283 nm.Results: The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.Conclusion: Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.

scholarly journals Identifikasi Isolat Antikanker Spons Hyrtios Erecta

2017 ◽  
Vol 10 (4) ◽  
pp. 123
Author(s):  
I MADE DIRA SWANTARA ◽  
WIWIK SUSANAH RITA ◽  
ANISA HERNINDYA
Keyword(s):  
Gas Chromatography ◽  
Anticancer Activity ◽  
Mass Spectroscopy ◽  
Ethanol Extract ◽  
Toxicity Screening ◽  
Activity Test ◽  
Cell Line Identification ◽  
Gas Chromatography Mass Spectroscopy ◽  
Hexanedioic Acid

ABSTRACTIsolation, anticancer activity test, and identification of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from Pari Island beach (Jakarta) has conducted. Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on BhrineShrimp Lethality Test (BSLT). In vitro anticancer activity test of the isolate was carried out using HeLa cell line. Identification of the compounds was performed by Gas chromatography-mass spectroscopy (GC-MS). Based on theresults, it was found that the toxic isolate of H. erecta sponges has anticancer activity with IC50 of 30,497 ppm. Four compounds was detected from the anticancer isolate i.e: 4-nonylphenol; dibutyl phthalate; hexanedioic acid bis(2-ethylhexyl) ester; and cholesterol. ABSTRAKTelah dilakukan isolasi, uji aktivitas antikanker, dan identifikasi isolat toksik yang berasal dari ekstrak etanol spons Hyrtios erecta yang diambil dari perairan Pulau Pari (Jakarta). Ekstraksi dilakukan dengan cara maserasi menggunakan etanol 70% pada temperatur kamar. Pemisahan dan pemurnian komponen menggunakan kromatografi kolom dengan fase diam silikagel dan fase gerak n-heksana-kloroform (2:8). Skrining toksisitas dilakukan dengan metode Bhrine Shrimp Lethality Test (BSLT). Uji antikanker secara in vitro isolat toksik tersebut menggunakan sel HeLa. Senyawanya diidentifikasi menggunakan Gas chromatography-mass spectroscopy (GC-MS). Berdasarkan hasil penelitian ini diperoleh bahwa isolat toksik spons H. erecta bersifat antikanker dengan IC50 sebesar 30,497 ppm. Pada isolat antikankertersebut terdeteksi empat senyawa, yaitu 4-nonylphenol; dibutil phtalat; ester heksadioat bis(2-etilheksil); dan kolesterol.

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