scholarly journals UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Ni Made Dwi Desiyanti ◽  
I Made Dira Swantara ◽  
I Putu Sudiarta
Keyword(s):  
Ir Spectra ◽  
Silica Gel ◽  
Column Chromatography ◽  
Myzus Persicae ◽  
Mobile Phase ◽  
Ethanol Extract ◽  
Annona Muricata ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Metabolite Extraction

The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.

scholarly journals Chemical Constituents of the Insecticidal Active Extract of Tinospora crispa

2019 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Nor Aziyah Bakhari ◽  
Siti Nur Amirah Diana Fadzillah ◽  
Norain Isa
Keyword(s):  
Blood Pressure ◽  
Petroleum Ether ◽  
Silica Gel ◽  
1H Nmr ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
First Time

Tinospora crispa Miers (Menispermaceae) is a climbing vine with stems rich in warts. The plant is called Akar Seruntum or Patawali in Malaysia and is widely used for treating skin complaints, malaria, bacterial abscess, high blood pressure and diabetes. In the present study, the stems of T. crispa were collected from the locality and succesively extracted with petroleum ether, followed by chloroform and ethanol. The insecticidal active extract (ethanol extract) was  subjected to column chromatography of silica gel eluted with a gradient mobile phase containing hexane, chloroform and ethanol. Among the chemical constituents isolated are n-tetracosyl trans-ferulate and n-octacosyl alcohol, along with three known aporphine alkaloids; N-formylnornuciferine, N-acetylnornuciferine and lysicamine. All compounds were identified by comparing their spectroscopic data (UV, IR, 1H NMR, MS) with data from corresponding values in the literature. Isolation of n-tetracosyl trans-ferulate and n-octacosyl alcohol is reported the first time for T. crispa.

scholarly journals Isolation and Identification of Anthralin From the Roots of Rhubarb Plant (Rheum palmatum)

2007 ◽  
Vol 4 (4) ◽  
pp. 546-549 ◽  
Author(s):  
A. Ashnagar ◽  
N. Gharib Naseri ◽  
H. Haidari Nasab
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Isolation And Identification ◽  
Polar Solvents ◽  
Rheum Palmatum

Anthralin is medically and chemically an important compound which can be found in rhubarb roots. Anthralin and anthralin derivatives have been used as antipsoriatic drugs. In this research, pure anthralin was isolated from the rhubarb roots by maceration and Soxhlet extraction methods in various polar and non-polar solvents. Successive TLC and column chromatography on silica gel with chloroform as the mobile phase afforded two distinct fractions with Rf= 0.54 and 0.61. The1HNMR,13CNMR, IR, UV and MS spectra showed that the fraction with Rf= 0.61 was anthralin. In both methods, methanol was found to be the most suitable solvent for extraction.

scholarly journals Karakterisasi Fraksi Aktif Antioksidan dari Ekstrak Etanol Biji Kemangi (Ocimum Basilicum L.)

2015 ◽  
pp. 39-49
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Chitta Putri Novianti
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Solvent Fractionation ◽  
Butanol Extract ◽  
Dpph Method ◽  
Ic50 Values

Characterization of antioxidant compounds from the seeds of basil (Ocimum basilicum L.) has been done. Extraction is done by maceration method using ethanol solvent, fractionation by TLC and column chromatography, antioxidants test using DPPH method, and characterization of antioxidant compound using GCMS. Ethanol extract and results of fractionation ethanol extract of basil seeds using n-hexane, ethyl acetate, n-butanol and methanol-water extracts show that n-butanol extract has the highest antioxidant activity with IC50 values ​​of 41.90 ppm. Results of column chromatography n-butanol extract using n-hexane : ethyl acetate (1:9) as mobile phase yielded 5 fractions with fraction 4 (F4 isolate) has dominant stain of active antioxidants after being sprayed DPPH reagent, it had IC50 values ​​of 39,70 ppm and total phenolic content of 0,003 mg/g. Isolate F4 suspected contains two active compounds as antioxidant which is terpenoid and phenolic compound group, namely squalene and 1,4-di-tert-buthyl-phenol identified by GCMS.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3598

scholarly journals ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta

2017 ◽  
Vol 1 (1) ◽  
pp. 16
Author(s):  
I Made Dira Swantara ◽  
Wiwik Susanah Rita ◽  
Rr Anisa Hernindy
Keyword(s):  
Stationary Phase ◽  
Anticancer Activity ◽  
Silica Gel ◽  
Screening Test ◽  
Mobile Phase ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Toxicity Screening ◽  
Polyphenol Compounds ◽  
Thousand Islands

AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The anticancer isolate contained alkaloid, steroid, and polyphenol compounds

scholarly journals Identification of the AntiListerialConstituents in Partially Purified Column Chromatography Fractions ofGarcinia kolaSeeds and Their Interactions with Standard Antibiotics

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
D. Penduka ◽  
L. Buwa ◽  
B. Mayekiso ◽  
A. K. Basson ◽  
A. I. Okoh
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Silica Gel ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Hexane Extract ◽  
The Other ◽  
Penicillin G ◽  
Garcinia Kola ◽  
Maximum Test

Partially purified fractions of the n-hexane extract ofGarcinia kolaseeds were obtained through column chromatography and their constituents were identified through the use of gas chromatography coupled to mass spectrometry (GC-MS). Three fractions were obtained by elution with benzene as the mobile phase and silica gel 60 as the stationery phase and these were named Benz1, Benz2, and Benz3 in the order of their elution. The antiListerialactivities of these fractions were assessed through MIC determination and only Benz2 and Benz3 were found to be active with MIC’s ranging from 0.625 to 2.5 mg/mL. The results of the GC-MS analysis showed Benz2 to have 9 compounds whilst Benz3 had 7 compounds, with the major compounds in both fractions being 9,19-Cyclolanost-24-en-3-ol, (3.β.) and 9,19-Cyclolanostan-3-ol,24-methylene-, (3.β.). The Benz2 fraction was found to have mainly indifferent interactions with ampicillin and penicillin G whilst mainly additive interactions were observed with ciprofloxacin. The Benz3 fraction’s interactions were found to be 50% synergistic with penicillin G and 25% synergistic with ciprofloxacin and ampicillin. A commercially available 9,19-Cyclolanost-24-en-3-ol, (3.β.) was found not to exhibit any antiListerialactivities at maximum test concentrations of 5 mg/mL, suggesting that the compound could be acting in synergy with the other compounds in the eluted fractions ofGarcinia kolaseeds.

scholarly journals Isolation and identification of chemical compounds from stem and roots of Sesbania sesban

2017 ◽  
Vol 6 (10) ◽  
pp. 1718 ◽  
Author(s):  
Baljinder Singh ◽  
Rajiv Sharma ◽  
Kalia A.N. ◽  
Vijender Kumar
Keyword(s):  
Column Chromatography ◽  
Spectroscopic Analysis ◽  
Chemical Compounds ◽  
Sesbania Sesban ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Rheumatic Conditions ◽  
Identification And Characterization

Sesbania sesban is a small perennial tree with woody stems, yellow flowers which is commonly known as Rawasan, family Leguminosae, is one of the restorative plant for inflammatory rheumatic conditions. This study on identification and characterization of isolated chemical compounds from n-butanol extract of root and stems of the plant S. sesban. This research is continued from previous research which is done by preliminary phytochemical screening and biological evaluations of extracts. The n-butanol extract was selected for compound isolation with column chromatography and identified by UV-VIS Spectrophotometer, FTIR, Mass and NMR Spectroscopic analysis. The results showed the isolated compounds identified as β-Sitosterol and Kaempferol.

scholarly journals PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS

2018 ◽  
Vol 11 (4) ◽  
pp. 133 ◽  
Author(s):  
Boudjada Amina ◽  
Touil Ahmed ◽  
Bendif Hamdi ◽  
Bensouici Chawki ◽  
Rhouati Salah
Keyword(s):  
Antioxidant Activity ◽  
Volatile Compounds ◽  
Silica Gel ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Isolation And Identification ◽  
Phenolic Contents ◽  
Butanol Extract ◽  
Chcl3 Extract ◽  
1H Nuclear Magnetic Resonance

 Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.

scholarly journals IDENTIFIKASI DAN UJI AKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK DAUN TREMBESI (Albizia saman (Jacq.) Merr) SEBAGAI ANTIBAKTERI Escherichia coli

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
I Kadek Pater Suteja ◽  
Wiwik Susanah Rita ◽  
I Wayan Gede Gunawan
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Column Chromatography ◽  
Ethanol Extract ◽  
E Coli ◽  
Butanol Extract ◽  
Medium Activity ◽  
Albizia Saman ◽  
Hydroxy Groups ◽  
Partition Process

Identification of flavonoid from the leaves of rain tree (Albizia saman (Jacq.) Merr) and its antibacterial activity test against Escherichia coli (E.coli) has been performed. This research aims to determine the type of flavonoid in rain tree leaves and its antibacterial activity against E. coli. Extraction was done by maceration and partition methods, separation was achieved by column chromatography, antibacterial activity was tested by disk diffussion method, and the identification was done by Ultraviolet-visible (UV-vis) and Infrared spectrophotometry. Extraction of 1 kg of rain tree powder with 5 L ethanol produced 73.38 g of concentrated ethanol extract. The partition process produced 26,34 g of n-hexane, 8,12 g of ethylacetate, 19,37 g n-butanol, and 12,56 g of water extracts. Phytochemical test of the extracts showed that the n-butanol extract contained flavonoids. Antibacterial activity of n-butanol extract towards E.coli showed a medium activity with a diameter inhibition of 6.3 mm. MIC value ??was 2% (w/v) with a diameter inhibition of 1 mm. Separation using column chromatography with the eluent of n-butanol: methanol: chloroform (5: 3: 2) obtained four isolates but only one isolate (isolate B with Rf 0.58) which contained flavonoids. Analysis with infrared spectroscopy showed that the isolate B contained functional groups of OH, C-OH, aliphatic CH, C = O ketones, C = C aromatic, COC ether, and aromatic CH. Analysis with UV-Vis spectrophotometer indicated that isolate B is isoflavon compound with a hydroxy groups at C-5 and C-7. The isolate was relatively pure by TLC. The isolate showed a low antibacterial activity.

scholarly journals Isolation and Identification of Pure Bioactive Compounds from Cuscuta reflexa Grown on Nerium oleander Host Plant by Flash Column Chromatography and GC-MS/MS Analysis

2020 ◽  
Vol 32 (10) ◽  
pp. 2486-2488
Author(s):  
Pooja Saini ◽  
Ekta Menghani ◽  
Rekha Mithal
Keyword(s):  
Host Plant ◽  
Bioactive Compounds ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Ethyl Acetate Extract ◽  
Nerium Oleander ◽  
Isolation And Identification ◽  
Cuscuta Reflexa ◽  
Flash Column Chromatography ◽  
Area Percentage

In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were identified in Cuscuta reflexa using NIST library on the basis of area percentage.

scholarly journals ISOLATION AND IDENTIFICATION OF MAIN AND SIDE PRODUCTS FROM ADDITION REACTION OF CARYOPHYLLENE OXIDE BY FORMIC ACID

2010 ◽  
Vol 2 (3) ◽  
pp. 155-160
Author(s):  
M Muchalal ◽  
Trisulistyaningsih Rahayu
Keyword(s):  
Gas Chromatography ◽  
Stationary Phase ◽  
Formic Acid ◽  
Silica Gel ◽  
Mobile Phase ◽  
Main Product ◽  
Addition Reaction ◽  
Caryophyllene Oxide ◽  
Isolation And Identification ◽  
Infrared Spectrophotometer

Main and side products from reaction of caryophyllene oxide and formic acid have been isolated using column chromatography with silica gel 60 G as stationary phase and n-hexane : ethyl acetat (9:1, v/v) as mobile phase. The first and second fraction were analyzed using infrared spectrophotometer, gas chromatography, and gas chromatography-mass spectrometer. Caryophyllene formiate as a main product was found in the first fraction. The side product that had been identified were 4,10,10-trimethyl-7-methylenebicyclo[6,2,0]decane-4-carbaldehyde, 2-(9-hydroxy-4,4,8 trimethyltricyclo[6,3,1,01,5]dodecyl)metanoate, clovanediol,4,11,11-trimethyl-8 methylene-3-bicyclo-[7,2,0]-undecen-5-ol,2,5-(6,10,10 imethyltricyclo[7,2,1,01,9]dodecyl)metanedioat, and derivative of caryophylleneformiate.   Keywords: Isolation, identification, caryophyllene oxide

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