scholarly journals Pre-biopsy modelling of condition of germinogenic epithelium in azoospermia using endocrine and genetic predictors

2017 ◽  
Vol 176 (5) ◽  
pp. 50-53
Author(s):  
S. Yu. Borovets ◽  
V. A. Toropov ◽  
S. Kh. Al'-Shukri
Keyword(s):  
Follicle Stimulating Hormone ◽  
Testicular Biopsy ◽  
Prognostic Models ◽  
Germinal Epithelium ◽  
Laboratory Studies ◽  
Nonobstructive Azoospermia ◽  
Prognostic Predictors ◽  
Genetic Predictors ◽  
Wide Range ◽  
Spermatogenic Epithelium

OBJECTIVE. The study revealed clinical, endocrine and genetical predictors of safety of spermatogenic epithelium in patients with nonobstructive azoospermia. MATERIAL AND METHODS. The open testicular biopsy was performed in 38 patients with secretory azoospermia. The wide range of instrumental, laboratory studies were conducted before biopsy. RESULTS. Two prognostic models were developed by taking into account the revealed predictors of damage of germinogenic epithelium. CONCLUSIONS. The age, concentration of follicle-stimulating hormone, presence or absence of microdeletion AZFc are independent prognostic predictors of germinal epithelium condition.

scholarly journals Differential Diagnosis of Azoospermia in Men with Infertility

2021 ◽  
Vol 10 (14) ◽  
pp. 3144
Author(s):  
Danilo L. Andrade ◽  
Marina C. Viana ◽  
Sandro C. Esteves
Keyword(s):  
Differential Diagnosis ◽  
Physical Examination ◽  
Detailed Analysis ◽  
Clinical Management ◽  
Semen Analysis ◽  
Testicular Biopsy ◽  
Nonobstructive Azoospermia ◽  
Sperm Cryopreservation ◽  
Imaging Studies ◽  
Detailed Medical History

The differential diagnosis between obstructive and nonobstructive azoospermia is the first step in the clinical management of azoospermic patients with infertility. It includes a detailed medical history and physical examination, semen analysis, hormonal assessment, genetic tests, and imaging studies. A testicular biopsy is reserved for the cases of doubt, mainly in patients whose history, physical examination, and endocrine analysis are inconclusive. The latter should be combined with sperm extraction for possible sperm cryopreservation. We present a detailed analysis on how to make the azoospermia differential diagnosis and discuss three clinical cases where the differential diagnosis was challenging. A coordinated effort involving reproductive urologists/andrologists, geneticists, pathologists, and embryologists will offer the best diagnostic path for men with azoospermia.

Male gonadal function after chemotherapy for solid tumors in childhood.

1989 ◽  
Vol 7 (3) ◽  
pp. 304-309 ◽  
Author(s):  
F Aubier ◽  
F Flamant ◽  
R Brauner ◽  
J M Caillaud ◽  
J M Chaussain ◽  
...  
Keyword(s):  
Toxic Effect ◽  
Alkylating Agents ◽  
Follicle Stimulating Hormone ◽  
Normal Growth ◽  
Testicular Biopsy ◽  
Testicular Function ◽  
Adult Males ◽  
Gonadal Function ◽  
Testicular Damage ◽  
Rule Out

The testicular function of 30 adolescent or adult males having undergone polychemotherapy in childhood was assessed by means of a spermogram or testicular biopsy. At the time of examination, the patients were pubertal and had completed chemotherapy between 1 and 20 years previously (mean, 9 years). All patients who were prepubertal or intrapubertal at the time of treatment achieved normal puberty with normal growth. Twenty patients presented with azoospermia and/or severe disturbances in the germinal line on biopsy. This series confirms the toxicity of alkylating agents, in particular that of the mechlorethamine, vincristine, procarbazine, and prednisone combination (MOPP) and that of cyclophosphamide (CPM). However, dactinomycin, vinblastine, and vincristine did not appear to have a toxic effect on spermatogenesis. The prepubertal state did not protect the gonads of 19 patients who were prepubertal at diagnosis: 12 are now sterile as a result of the treatment. An increase in basal follicle-stimulating hormone (FSH) levels gives a good indication of testicular damage, although normal levels do not rule out the possibility of azoospermia.

scholarly journals Some notes on a historical perspective of panic disorder

2006 ◽  
Vol 55 (2) ◽  
pp. 154-160 ◽  
Author(s):  
Antonio Egidio Nardi
Keyword(s):  
Panic Disorder ◽  
20Th Century ◽  
19Th Century ◽  
Greek Mythology ◽  
Laboratory Studies ◽  
Biochemical Tests ◽  
Wide Range ◽  
Therapeutic Development ◽  
History Of ◽  
The 19Th Century

This article aims to describe important points in the history of panic disorder concept, as well as to highlight the importance of its diagnosis for clinical and research developments. Panic disorder has been described in several literary reports and folklore. One of the oldest examples lies in Greek mythology - the god Pan, responsible for the term panic. The first half of the 19th century witnessed the culmination of medical approach. During the second half of the 19th century came the psychological approach of anxiety. The 20th century associated panic disorder to hereditary, organic and psychological factors, dividing anxiety into simple and phobic anxious states. Therapeutic development was also observed in psychopharmacological and psychotherapeutic fields. Official classifications began to include panic disorder as a category since the third edition of the American Classification Manual (1980). Some biological theories dealing with etiology were widely discussed during the last decades of the 20th century. They were based on laboratory studies of physiological, cognitive and biochemical tests, as the false suffocation alarm theory and the fear network. Such theories were important in creating new diagnostic paradigms to modern psychiatry. That suggests the need to consider a wide range of historical variables to understand how particular features for panic disorder diagnosis have been developed and how treatment has emerged.

Concluding Speculations

2019 ◽  
pp. 334-344
Author(s):  
Mari Riess Jones
Keyword(s):  
Natural Environments ◽  
Laboratory Studies ◽  
Control Stimulus ◽  
Wide Range ◽  
Stimulus Timing

This final chapter is speculative. It addresses the entrainment assumptions of universality and resonance by reviewing the natural environments of a range of different species, from crickets to whales. The idea is that sensitivity to both rate and rhythm across different species is support for assuming natural driving rhythms exist that facilitate entrainment in a wide range of other species. Also discussed are two artifactual environments experienced by humans: namely, that of laboratory studies that artificially control stimulus timing and the contemporary machine-driven environment of computers and iPhones. The latter are speculated to lead to a sporadic habitat based on fast irregular (artifactual) driving rhythms.

scholarly journals Testosterone and Gonadotropins in Infertile Men with Sertoli Cell Only Syndrome from Gaza Strip

2017 ◽  
Vol 18 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Maged M Yassin ◽  
Abdel Monem H Lubbad ◽  
Ahmed Z Taha ◽  
Mohammed M Laqqan ◽  
Samar M Abu Jamiea
Keyword(s):  
Sertoli Cell ◽  
Gaza Strip ◽  
Follicle Stimulating Hormone ◽  
Reference Value ◽  
Serum Testosterone ◽  
Testicular Biopsy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hypogonadotrophic Hypogonadism ◽  
Infertile Men ◽  
Normal Reference Value

Aim: To assess serum testosterone and gonadotropins in Sertoli cell only syndrome patients from Gaza Strip.Methods: Based on testicular biopsy, a cross section of 74 Sertoli cell only syndrome patients were enrolled in the study. Age matched 44 fertile men were served as controls. Patients and controls were questioned for their medical history. Blood samples were drawn and serum testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) were determined by enzyme-linked immunosorbent assay. Data were computer analyzed using SPSS/PC, version 18.0.Results: Varicocele and hormonal problems were significantly more frequent among patients than controls (P<0.05). Serum testosterone was significantly lower in patients compared to controls (1.7±1.3 versus 5.0±2.2 ng/ml, P=0.000). In contrast, LH and FSH were significantly higher in patients than controls (12.8±9.7 and 20.8±14.8 mlU/ml versus 6.3±3.1 and 7.7±3.9 mlU/ml, P=0.000, respectively). Hypergonadotrophic hypogonadism and hypogonadotrophic hypogonadism patients showed lower levels of testosterone compared to the normal reference value (0.9±0.5 and 0.5±0.4 ng/ml versus 2.0-7.0 ng/ml). Higher levels of LH and FSH were recorded in hypergonadotrophic hypogonadism (24.5±2.6 and 37.4±6.7 mlU/ml) compared to the reference values of 2.0-13.0 and 2.5-10.0 mlU/ml, respectively whereas LH and FSH levels were lower in hypogonadotrophic hypogonadism (0.6±0.4 and 0.6±0.5 mlU/ml, respectively). In this context, all hypergonadotrophic hypogonadism and hypogonadotrophic hypogonadism patients showed abnormal levels of testosterone, LH and FSH.Conclusions: Abnormal levels of serum testosterone, LH and FSH, particularly in hypergonadotrophic hypogonadism and hypogonadotrophic hypogonadism were identified in infertile men with Sertoli cell only syndrome from Gaza Strip.J MEDICINE January 2017; 18 (1) : 21-26

scholarly journals Transfer of a Multiclass Method for over 60 Antibiotics in Food from High Resolution to Low Resolution Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2935 ◽  
Author(s):  
Giusepponi ◽  
Paoletti ◽  
Barola ◽  
Moretti ◽  
Saluti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Development Process ◽  
Tandem Mass ◽  
Laboratory Studies ◽  
Low Resolution ◽  
Method Transfer ◽  
Wide Range ◽  
Microbial Residues

A multiclass method has been developed to screen and confirm a wide range of anti-microbial residues in muscle and milk, and validated using liquid-chromatography coupled to (low-resolution, LR) tandem mass spectrometry (LC-QqQ). Over sixty antibiotics, belonging to ten distinct families, were included in the method scope. The development process was rapidly concluded as a result of two previously implemented methods. This consisted of identical sample treatments, followed by liquid chromatography, and coupled with high-resolution (HR) mass spectrometry (LC-Q-Orbitrap). The validation study was performed in the range between 10–1500 μg·kg−1 for muscles and 2–333 μg·kg−1 for milk. The main performance characteristics were estimated and, then, compared to those previously obtained with HR technique. The validity of the method transfer was ascertained also through inter-laboratory studies.

scholarly journals Varicocele Repair Improves Testicular Histology in Men with Nonobstructive Azoospermia

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Murat Ustuner ◽  
Hasan Yilmaz ◽  
Ufuk Yavuz ◽  
Seyfettin Ciftci ◽  
Ali Saribacak ◽  
...  
Keyword(s):  
Extraction Procedure ◽  
Testicular Biopsy ◽  
Testicular Tissue ◽  
High Serum ◽  
Histopathological Analysis ◽  
Nonobstructive Azoospermia ◽  
Biopsy Specimens ◽  
Testicular Histology ◽  
Late Maturation ◽  
Varicocele Repair

Objective. To determine the histopathological differences after varicocele repair in testicular tissue in males with nonobstructive azoospermia.Methods. Between 2009 and 2014, 45 men with complete azoospermia and palpable varicocele, presenting with primary infertility of at least 1 year, undergoing varicocele repair at our institution were selected for the study. A standard systematic testicular 6-core Tru-Cut biopsy was performed during varicocele repair. Other biopsies were obtained from each testicle of all patients at the time of microscopic sperm extraction procedure.Results. Nineteen patients were selected for the study. Testicular biopsy specimens were classified as Sertoli cell only on preoperative histopathological analysis in 14 patients. After varicocele repair, focal spermatogenesisn=3and late maturation arrestn=2were found in these patients. Average Johnsen score was significantly increased after varicocelectomyP=0.003. Motile sperm was found in one patient on postoperative semen analyses and in 10 more patients in the microscopic sperm extraction procedure. Preoperative high serum follicle stimulating hormone level and venous reflux were significantly and negatively correlated with the increase in average Johnsen scoreP<0.05.Conclusions. Our findings suggest significant improvement in testicular histology after varicocele repair.

Pituitary hyperplasia with Sertoli cell-only and 47,XYY syndromes: an uncommon triad

2020 ◽  
Vol 13 (5) ◽  
pp. e233100
Author(s):  
Amelle Geurim Ra ◽  
Paul Jeffrey Evans ◽  
Anshu Awasthi ◽  
Upendram Srinivas-Shankar
Keyword(s):  
Sertoli Cell ◽  
Semen Analysis ◽  
Follicle Stimulating Hormone ◽  
Testicular Biopsy ◽  
In Vitro Fertilisation ◽  
Donor Sperm ◽  
Healthy Baby ◽  
Pituitary Hyperplasia ◽  
History Of

We report the case history of a 32-year-old man with no phenotypical abnormalities who presented with infertility. Semen analysis revealed azoospermia and testicular biopsy confirmed Sertoli cell-only (SCO) syndrome. Karyotyping revealed 47,XYY and pituitary hyperplasia was found on MRI pituitary. In our patient, 47,XYY karyotype is likely to have given rise to SCO syndrome that in turn resulted in pituitary hyperplasia. The patient was evaluated by various members of the multidisciplinary team including the pituitary surgeon, endocrinologist and andrologist. The patient’s partner successfully delivered a healthy baby via in vitro fertilisation with donor sperm. This triad of diagnoses (SCO syndrome, 47,XYY karyotype and pituitary hyperplasia) has not been reported previously. SCO syndrome should be considered in the presence of azoospermia, elevated follicle-stimulating hormone, low inhibin-B and normal testosterone levels. Our case report also highlights the importance of excluding genetic causes of infertility even when the patient has no phenotypical abnormalities.

Relationship between testicular morphology and sperm production following ischaemia in the ram

1995 ◽  
Vol 7 (1) ◽  
pp. 119 ◽  
Author(s):  
CM Markey ◽  
AM Jequier ◽  
GT Meyer ◽  
GB Martin
Keyword(s):  
Morphological Changes ◽  
Sperm Concentration ◽  
Testicular Biopsy ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Infertile Men ◽  
Testicular Morphology ◽  
And Function ◽  
The Relationship ◽  
Spermatogenic Epithelium

Arteriosclerosis was induced in the internal spermatic artery of rams to determine if this condition is implicated in the aetiology of testicular pathology which causes male infertility. Data were collected on sperm concentration and motility for 56 days following surgery to provide an index of testicular function. Testes were then weighed and a testicular biopsy score count was performed on histological sections to assess spermatogenic potential of seminiferous tubules. Vascular disturbance caused focal damage of the seminiferous epithelium, similar to that seen among infertile men, and a reduction in ejaculate volume, sperm concentration and sperm motility. Sperm concentration decreased following ischaemia yet was maintained to some degree by a germ-cell depleted spermatogenic epithelium. Normal testicular morphology was maintained above a testis weight of about 120 g (for an individual testis), but below this threshold spermatogenesis was severely impaired. In conclusion, these data have provided information on the relationship between testicular morphology and function following ischaemia in the ram. Furthermore, the morphological changes induced in the testis were similar to those seen among infertile men and, by their focal nature, could explain the distinction between oligozoospermia and azoospermia in men exhibiting spermatogenic arrest.

EVALUATION OF NONOBSTRUCTIVE AZOOSPERMIA WITH BILATERAL TESTICULAR BIOPSY

1999 ◽  
pp. 342 ◽  
Author(s):  
Murat Samli ◽  
Oguzhan Sariyuce ◽  
Murat Basar ◽  
Tulay Evrenkaya
Keyword(s):  
Testicular Biopsy ◽  
Nonobstructive Azoospermia
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