scholarly journals Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA

2019 ◽  
Vol 7 (9) ◽  
pp. 1465-1468
Author(s):  
Elif Canpolat ◽  
Burcu Biterge-Süt
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Local Source ◽  
Local Alignment ◽  
Sequencing Analysis ◽  
Cobalt Chromium ◽  
Isolation And Characterization ◽  
Rdna Sequencing

Microbial and chemical contamination is one of the biggest environmental problems since exposure to pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, are often associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultative anaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, we isolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area and therefore presents a great source of contamination. The bacterial isolate was grown, colony morphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencing analysis, which was performed using DNA samples isolated from pure cultures and amplified by polymerase chain reaction (PCR). Sequencing results were compared against known bacterial strains using BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against 20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper, iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method. Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our results indicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive against others. Similarly, the bacteria were resistant against high concentrations of most metals tested. In summary, this study provided the first data regarding the isolation and characterization of Paenibacillus lautus strain from a local source in Turkey, which will account as preliminary data and guide our future efforts to fight against microbial contaminations.

scholarly journals Isolation and Molecular Identification of Virulence, Antimicrobial and Heavy Metal Resistance Genes in Livestock-Associated Methicillin-Resistant Staphylococcus aureus

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 79 ◽  
Author(s):  
Chumisa C. Dweba ◽  
Oliver T. Zishiri ◽  
Mohamed E. El Zowalaty
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Staphylococcus Aureus ◽  
South African ◽  
Resistance Genes ◽  
Heavy Metal Resistance ◽  
Livestock Production ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Methicillin Resistant

Staphylococcus aureus is one of the most important pathogens of humans and animals. Livestock production contributes a significant proportion to the South African Gross Domestic Product. Consequently, the aim of this study was to determine for the first time the prevalence, virulence, antibiotic and heavy metal resistance in livestock-associated S. aureus isolated from South African livestock production systems. Microbial phenotypic methods were used to detect the presence of antibiotic and heavy metal resistance. Furthermore, molecular DNA based methods were used to genetically determine virulence as well as antibiotic and heavy metal resistance determinants. Polymerase chain reaction (PCR) confirmed 217 out of 403 (53.8%) isolates to be S. aureus. Kirby-Bauer disc diffusion method was conducted to evaluate antibiotic resistance and 90.8% of S. aureus isolates were found to be resistant to at least three antibiotics, and therefore, classified as multidrug resistant. Of the antibiotics tested, 98% of the isolates demonstrated resistance towards penicillin G. High resistance was shown against different heavy metals, with 90% (196/217), 88% (192/217), 86% (188/217) and 84% (183/217) of the isolates resistant to 1500 µg/mL concentration of Cadmium (Cd), Zinc (Zn), Lead (Pb) and Copper (Cu) respectively. A total of 10 antimicrobial resistance and virulence genetic determinants were screened for all livestock associated S. aureus isolates. Methicillin-resistant S. aureus (MRSA) isolates were identified, by the presence of mecC, in 27% of the isolates with a significant relationship (p < 0.001)) with the host animal. This is the first report of mecC positive LA-MRSA in South Africa and the African continent. The gene for tetracycline resistance (tetK) was the most frequently detected of the screened genes with an overall prevalence of 35% and the highest prevalence percentage was observed for goats (56.76%) followed by avian species (chicken, duck and wild birds) (42.5%). Virulence-associated genes were observed across all animal host species. The study reports the presence of luks/pv, a gene encoding the PVL toxin previously described to be a marker for community acquired-MRSA, suggesting the crossing of species between human and livestock. The high prevalence of S. aureus from the livestock indicates a major food security and healthcare threat. This threat is further compounded by the virulence of the pathogen, which causes numerous clinical manifestations. The phenomenon of co-selection is observed in this study as isolates exhibited resistance to both antibiotics and heavy metals. Further, all the screened antibiotic and heavy metal resistance genes did not correspond with the phenotypic resistance.

scholarly journals Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Güllük Bay, Turkey : Quantifying the resistance of identified bacteria species with potential for environmental remediation applications

2020 ◽  
Vol 64 (4) ◽  
pp. 507-525 ◽  
Author(s):  
Gülşen Altuğ ◽  
Mine Çardak ◽  
Pelin Saliha Çiftçi Türetken ◽  
Samet Kalkan ◽  
Sevan Gürün
Keyword(s):  
Heavy Metal ◽  
Aegean Sea ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Heavy Metal Resistant ◽  
Seawater Samples

Heavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK® 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.

scholarly journals Surface water isolates of hemolytic and non-hemolytic Acinetobacter with multiple drug and heavy metal resistance ability

2013 ◽  
Vol 12 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Sevilay Akbulut ◽  
Fadime Yilmaz ◽  
Bulent Icgen
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Surface Water ◽  
Pathogenic Bacteria ◽  
Rapid Development ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Rdna Sequencing ◽  
Wide Range ◽  
Multiple Drugs

Acinetobacter in surface waters are a major concern because of their rapid development of resistance to a wide range of antimicrobials and their ability to persist in these waters for a very long time. Four surface water isolates of Acinetobacter having both multidrug- and multimetal-resistant ability were isolated and identified through biochemical tests and 16S rDNA sequencing. Based on these analyses, two hemolytic isolates were affiliated with Acinetobacter haemolyticus with an accession number of X81662. The other two non-hemolytic isolates were identified as Acinetobacter johnsonii and Acinetobacter calcoaceticus and affiliated with accession numbers of Z93440 and AJ888983, respectively. The antibiotic and heavy metal resistance profiles of the isolates were determined by using 26 antibiotics and 17 heavy metals. Acinetobacter isolates displayed resistance to β-lactams, cephalosporins, aminoglycosides, and sulfonamides. The hemolytic isolates were found to show resistance to higher numbers of heavy metals than the non-hemolytic ones. Due to a possible health risk of these pathogenic bacteria, a need exists for an accurate assessment of their acquired resistance to multiple drugs and metals.

scholarly journals Characterisation of surfactant-expressing bacteria and their potential bioremediation properties from hydrocarbon-contaminated and uncontaminated soils

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Raji Bamanga ◽  
Yusuf Y. Deeni ◽  
Andrew Spiers ◽  
Scott Cameron
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Phenotypic Diversity ◽  
Draft Genome ◽  
Hierarchical Cluster ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Ex Situ ◽  
Rdna Sequencing ◽  
High Concentrations

We are investigating characteristics associated with oil degradation amongst bacteria isolated from clean and hydrocarbon contaminated soils from Nigeria and the UK.Our focus has been to identify bacteria expressing surfactants following isolation on Pseudomonas selective (PSA-CFC) and non-selective nutrient media and investigate the nature of surfactants, heavy metal resistance and hydrocarbon-degrading enzymes expressed by the bacteria. Of five sites sampled, a total of 1460 colonies were tested using the drop collapse assay, and 110 were found to express surfactants reducing liquid surface tensions as assessed by quantitative tensiometry to between 24.7 and 26.7 mN.m-1 (Tukey-Kramer HSD, α=0.05). We undertook a range of growth and behaviour-based assays on 60 selected strain which, when investigated by Hierarchical cluster analysis (HCA) demonstrated that this collection showed considerable phenotypic diversity. Eight out of the 60 strains could grow at a high temperature (50 °C), 35 of the 60 strains utilized diesel as a sole carbon source, and most of the strains could tolerate high concentrations (up to 20 mM) of heavy metals. Identification by 16S rDNA sequencing revealed that some of the strains belong to Pseudomonas, Bacillus, and Stenotrophomonas genera. We found using bioinformatics analysis of eight-selected draft genome sequences (AntiSMASH and RAST) NRPS-like (probable surfactants), cytochrome P450, catechol-1,2/2,3-dioxygenase, lipase, and heavy metal resistance gene sequences. We intend to use the information provided in this research to select strains for potential applications in in-situor ex-situ bioremediation of hydrocarbon-contaminated soils.

scholarly journals Heavy Metal Tolerance Trend in Extended-Spectrum β-Lactamase Encoding Strains Recovered from Food Samples

2021 ◽  
Vol 18 (9) ◽  
pp. 4718
Author(s):  
Kashaf Junaid ◽  
Hasan Ejaz ◽  
Iram Asim ◽  
Sonia Younas ◽  
Humaira Yasmeen ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Food Samples ◽  
Esbl Production ◽  
Extended Spectrum ◽  
Retail Food ◽  
Esbl Producers

This study evaluates bacteriological profiles in ready-to-eat (RTE) foods and assesses antibiotic resistance, extended-spectrum β-lactamase (ESBL) production by gram-negative bacteria, and heavy metal tolerance. In total, 436 retail food samples were collected and cultured. The isolates were screened for ESBL production and molecular detection of ESBL-encoding genes. Furthermore, all isolates were evaluated for heavy metal tolerance. From 352 culture-positive samples, 406 g-negative bacteria were identified. Raw food samples were more often contaminated than refined food (84.71% vs. 76.32%). The predominant isolates were Klebsiella pneumoniae (n = 76), Enterobacter cloacae (n = 58), and Escherichia coli (n = 56). Overall, the percentage of ESBL producers was higher in raw food samples, although higher occurrences of ESBL-producing E. coli (p = 0.01) and Pseudomonas aeruginosa (p = 0.02) were observed in processed food samples. However, the prevalence of ESBL-producing Citrobacter freundii in raw food samples was high (p = 0.03). Among the isolates, 55% were blaCTX-M, 26% were blaSHV, and 19% were blaTEM. Notably, heavy metal resistance was highly prevalent in ESBL producers. These findings demonstrate that retail food samples are exposed to contaminants including antibiotics and heavy metals, endangering consumers.

scholarly journals Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus

2021 ◽  
Vol 9 (3) ◽  
pp. 499
Author(s):  
Majid Rasool Kamli ◽  
Nada A. Y. Alzahrani ◽  
Nahid H. Hajrah ◽  
Jamal S. M. Sabir ◽  
Adeel Malik
Keyword(s):  
Heavy Metal ◽  
Enzyme Activities ◽  
Heavy Metal Resistance ◽  
Industrial Applications ◽  
Metal Resistance ◽  
The Family ◽  
Antiviral Activities ◽  
Genome Level ◽  
Genome Analyses ◽  
Unique Species

Bacteria belonging to the genus Aneurinibacillus within the family Paenibacillaceae are Gram-positive, endospore-forming, and rod-shaped bacteria inhabiting diverse environments. Currently, there are eight validly described species of Aneurinibacillus; however, several unclassified species have also been reported. Aneurinibacillus spp. have shown the potential for producing secondary metabolites (SMs) and demonstrated diverse types of enzyme activities. These features make them promising candidates with industrial implications. At present, genomes of 9 unique species from the genus Aneurinibacillus are available, which can be utilized to decipher invaluable information on their biosynthetic potential as well as enzyme activities. In this work, we performed the comparative genome analyses of nine Aneurinibacillus species representing the first such comprehensive study of this genus at the genome level. We focused on discovering the biosynthetic, biodegradation, and heavy metal resistance potential of this under-investigated genus. The results indicate that the genomes of Aneurinibacillus contain SM-producing regions with diverse bioactivities, including antimicrobial and antiviral activities. Several carbohydrate-active enzymes (CAZymes) and genes involved in heavy metal resistance were also identified. Additionally, a broad range of enzyme classes were also identified in the Aneurinibacillus pan-genomes, making this group of bacteria potential candidates for future investigations with industrial applications.

scholarly journals Retromobilization of heavy metal resistance genes in unpolluted and heavy metal polluted soil

1995 ◽  
Vol 18 (3) ◽  
pp. 191-203 ◽  
Author(s):  
Eva M. Top ◽  
Helene Rore ◽  
Jean-Marc Collard ◽  
Veerle Gellens ◽  
Galina Slobodkina ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Polluted Soil

Screening of heavy metal and antibiotic resistance among Proteus vulgaris isolates from hospital wastewater of Northern India

2021 ◽  
Vol 19 ◽  
Author(s):  
Manzar Alam ◽  
Mohd Imran ◽  
Syed Sayeed Ahmad
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Multiple Drug Resistance ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Proteus Vulgaris ◽  
Resistance To Antibiotics ◽  
Transfer Frequency ◽  
Resistance Patterns

Background: Microbial resistance to antibiotics and heavy metals is a rising problem in the world today. All the Proteus vulgaris isolates showed their MIC in between 50-1600 µg/ml. Of 70% and 46% of the isolates showed their MIC at 800-1200 µg/ml against Zn2+ and Cu2+ while 80% of the isolates showed their MIC at 100-200 µg/ml against Ni2+, respectively. All Proteus vulgaris isolates also exhibited multiple resistance patterns (2-7 heavy metals) in different combination of metals. The Multi metal resistance Index (MHMR) ranges were found (0.04-0.5). Methods: A high level of antibiotics resistance was observed against Methicillin (100%) and least to Oflaxicin (6%), Gentamycine and Neomycin (10%). All Proteus vulgaris isolates also showed multiple drug resistance patterns (2-12 antibiotics) in different combination of antibiotics. The MAR index ranges were found (0.02-0.7). Of 98%, 84% and 80% of the total isolates showed urease, gelatinase and amylase activity. Results: The Proteus vulgaris isolates contained plasmid of size ranging from 42.5 to 57.0kb and molecular weight of plasmids ranged from 27.2 to 37.0 MD. Incidences of resistance transfer, 7 pairs of isolates were assessed for the transfer of the antibiotic/ heavy metal resistance markers. The higher (4.4x10-1 and 3.4x10-1) transfer frequency was observed among antibiotic and heavy metal while lower transfer frequency were (5.0x10-2 and 1.0x10-2) showed against antibiotic and heavy metal in both the medium from the entire site tested, respectively. Conclusion: Indicating the high threat of environmental pollution and appearance of heavy metal resistance which may support the enlargement of resistance to antibiotics among the pathogens.

scholarly journals A Review on the Resistance and Accumulation of Heavy Metals by Different Microbial Strains

2022 ◽  
Author(s):  
Madhuri Girdhar ◽  
Zeba Tabassum ◽  
Kopal Singh ◽  
Anand Mohan
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Volcanic Eruptions ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Contaminated Sites ◽  
Bacillus Species ◽  
Polluted Sites ◽  
Pollution Accumulation ◽  
Microbial Strains

Heavy metals accumulated the earth crust and causes extreme pollution. Accumulation of rich concentrations of heavy metals in environments can cause various human diseases which risks health and high ecological issues. Mercury, arsenic, lead, silver, cadmium, chromium, etc. are some heavy metals harmful to organisms at even very low concentration. Heavy metal pollution is increasing day by day due to industrialization, urbanization, mining, volcanic eruptions, weathering of rocks, etc. Different microbial strains have developed very efficient and unique mechanisms for tolerating heavy metals in polluted sites with eco-friendly techniques. Heavy metals are group of metals with density more than 5 g/cm3. Microorganisms are generally present in contaminated sites of heavy metals and they develop new strategies which are metabolism dependent or independent to tackle with the adverse effects of heavy metals. Bacteria, Algae, Fungi, Cyanobacteria uses in bioremediation technique and acts a biosorbent. Removal of heavy metal from contaminated sites using microbial strains is cheaper alternative. Mostly species involved in bioremediation include Enterobacter and Pseudomonas species and some of bacillus species too in bacteria. Aspergillus and Penicillin species used in heavy metal resistance in fungi. Various species of the brown algae and Cyanobacteria shows resistance in algae.

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